scholarly journals Non-Thermal Atmospheric Pressure Argon-Sourced Plasma Flux Promotes Wound Healing of Burn Wounds and Burn Wounds with Infection in Mice through the Anti-Inflammatory Macrophages

2021 ◽  
Vol 11 (12) ◽  
pp. 5343
Author(s):  
Cong Phi Dang ◽  
Sirapong Weawseetong ◽  
Awirut Charoensappakit ◽  
Kritsanawan Sae-Khow ◽  
Decho Thong-Aram ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Plasma Flux ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Anti Inflammatory ◽  
Inflammatory Macrophages ◽  
The Impact

Plasma medicine is the utilization of gas ionization that might be beneficial for the treatment of burn wounds, a healthcare problem with a significant mortality rate. Due to a lack of information on the impact of plasma flux in immune cells and a high prevalence of bacterial infection in burn wounds, non-thermal argon-based plasma flux was tested on macrophages (RAW246.7) and in mouse models of burn wounds with or without Staphylococcus aureus infection. Accordingly, plasma flux enhanced reactive oxygen species (ROS), using dihydroethidium assay, and decreased abundance of NF-κB-p65 (Western blot analysis) in non-stimulating macrophages. In parallel, plasma flux upregulated IL-10 gene expression (an anti-inflammatory cytokine) in lipopolysaccharide (LPS)-induced inflammatory macrophages, while downregulating the pro-inflammatory cytokines (IL-1β and IL-6). Additionally, plasma flux improved the migratory function of fibroblasts (L929) (fibroblast scratch assay) but not fibroblast proliferation. Moreover, once daily plasma flux administration for 7 days promoted the healing process in burn wounds with or without infection (wound area and wound rank score). Additionally, plasma flux reduced tissue cytokines (TNF-α and IL-6) in burn wounds with infection and promoted collagen in burn wounds without infection. In conclusion, plasma flux induced anti-inflammatory macrophages and promoted the burn-wound healing process partly through the decrease in macrophage NF-κB. Hence, plasma flux treatment should be tested in patients with burn wounds.

An Overview of the Most Important Medicinal Plants Native to Iran by Property of Burn Wound Healing: A Systematic Review

2017 ◽  
Vol 8 (02) ◽  
Author(s):  
Mehrdad Karimi ◽  
Mahnaz Mardani ◽  
Leila Mahmoodnia
Keyword(s):  
Wound Healing ◽  
Medicinal Plants ◽  
Cinnamic Acid ◽  
Healing Process ◽  
Review Article ◽  
The Body ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing

Burn is an injury where the skin is destroyedby various factors such as heat, cold, electricity, etc.When skin injuryoccurs, pathogens can invade the body and burn wounds get infected shortly after the damage. The wound healing process is a process done by the coordination of tissues, cells and various factors. In the acute phase, inflammatory mechanism of burns has negative effects due to capillary leak. On the other hand, in the later stages of burn,inflammation is necessary for wound healing. Silver nitrate, silver sulfadiazine, and Mafenide acetate are also used for wound care. These compounds cause delayed wound healing and have cytotoxic effects. Iran is among the countries that have rich traditional medicine and herbal treatment of burn wounds in history. Therefore, in this review article, we tried to report the medicinal plants native to Iran which are effective for burn wounds. In this review article, by searching databases such as Scopus, Google Scholar, Spy DVD, mag Iran and… the articles were searched by searching keywords including burn wounds, herbs, extracts, essences and Iran. After finally searching, it was determined that medicinal plants below are the most important medicinal plants native to Iran that affect burn wounds. 11 Medicinal herbs 11 Abukhalsa plant, chamomile, oak, hungry for love, green tea, purslane, cinnamon, Hypericum, and Commiphora are the most important Medicinal Plants which are effective for burn wound healing in Iran. Alkanin, Naphthoquinone and Shikonin, tannins, pectin, alkaloids, saponins and tannins fixed, resin and vitamin C, flavonoids rutin, aucubin and catalpolCatechin, Epicatechin, EpicatechinGallate, Epigallocatechin and Epigallocatechin-gallate, oxalic acid, Cinnamic acid, caffeic acid , maleic acid, Citric acid, coumarin, flavonoids, alanine, tannins, alpha-linolenic acid, cinnamic acid, phenolic compounds such as eugenol, and safrolephellandrene, terpene compounds such as limonene and linalool, trans-cinnamaldehyde (MDA), tannins, coumarin, resin, Cinema-hydroxy phenyl propane compounds such as formaldehyde and mannitol, curcumin, terpenoids, steroids, Flavonoids, lignans are active compounds of plant bioactive substances that are effective on burn wound healing.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

scholarly journals Bee Pollen: Chemical Composition and Therapeutic Application

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Katarzyna Komosinska-Vassev ◽  
Pawel Olczyk ◽  
Justyna Kaźmierczak ◽  
Lukasz Mencner ◽  
Krystyna Olczyk
Keyword(s):  
Wound Healing ◽  
Pathological Condition ◽  
Healing Process ◽  
Radical Scavenging ◽  
Wound Healing Process ◽  
Therapeutic Application ◽  
Burn Wound ◽  
Bee Pollen ◽  
Burn Wound Healing ◽  
Natural Medicine

Bee pollen is a valuable apitherapeutic product greatly appreciated by the natural medicine because of its potential medical and nutritional applications. It demonstrates a series of actions such as antifungal, antimicrobial, antiviral, anti-inflammatory, hepatoprotective, anticancer immunostimulating, and local analgesic. Its radical scavenging potential has also been reported. Beneficial properties of bee pollen and the validity for their therapeutic use in various pathological condition have been discussed in this study and with the currently known mechanisms, by which bee pollen modulates burn wound healing process.

scholarly journals Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Pawel Olczyk ◽  
Katarzyna Komosinska-Vassev ◽  
Grzegorz Wisowski ◽  
Lukasz Mencner ◽  
Jerzy Stojko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Burn Treatment ◽  
Burn Wound Healing ◽  
Fibronectin Expression ◽  
The Matrix ◽  
Healing Wounds

The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.

scholarly journals Topical treatment of standardised burns with herbal remedies in model rats

2005 ◽  
Vol 5 (4) ◽  
pp. 50-57 ◽  
Author(s):  
Fahir Bečić ◽  
Nedžad Mulabegović ◽  
Zakira Mornjaković ◽  
Elvedina Kapić ◽  
Senad Prašović ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Herbal Remedies ◽  
Control Group ◽  
Therapeutic Effects ◽  
Skin Area ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

Topical Insulin Modulates Inflammatory and Proliferative Phases of Burn-Wound Healing in Diabetes-Induced Rats

2019 ◽  
Vol 21 (5) ◽  
pp. 473-484 ◽  
Author(s):  
Flávia Figueiredo Azevedo ◽  
Gabriela Virgínia Moreira ◽  
Caio Jordão Teixeira ◽  
Ana Flávia Marçal Pessoa ◽  
Michele Joana Alves ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Healing Process ◽  
Elastic Fibers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Inflammatory Phase ◽  
Burn Wound Healing

The healing time of burn wounds depends on surface area and depth of the burn and associated comorbidities. Diabetes mellitus (DM) causes delays in the healing process by extending the inflammatory phase. Treatment with topical insulin can improve the inflammatory phase, restore metabolic dysregulation, and modulate impaired cellular signaling in burn wounds. The objective of this study was to evaluate markers of the inflammatory and proliferative phases of second-degree burns after topical insulin treatment in diabetic rats. Type I DM was induced with streptozotocin in male Wistar rats. The animals’ backs were shaved and subjected to thermal burning. Rats were randomized into two groups: control diabetic (DC) and insulin diabetic (DI). At Days 7 and 14 postburn, rats were euthanized, and wound-tissue sections were evaluated by hematoxylin and eosin, Weigert, and Verhöeff staining, immunohistochemistry-paraffin, and enzyme-linked immunosorbent assay. A significant increase in reepithelialization was seen on Days 7 and 14 in DI versus DC rats. On Day 7, interleukin (IL)-1β, IL-6, monocyte chemotactic protein (MCP)-1, and F4/80 expression were increased in DI versus DC rats. On Day 14, MCP-1 expression was decreased and F4/80 increased in DI versus DC rats. On Days 7 and 14, Ki-67, transforming growth factor-β1, vascular endothelial growth factor expression, and formation of elastic fibers were increased in DI versus DC rats. Topical insulin modulates burn-wound healing in diabetic animals by balancing inflammation and promoting angiogenesis and formation of elastic fibers.

scholarly journals Role of topical Sucralfate in healing of burn wounds

2018 ◽  
Vol 5 (9) ◽  
pp. 2995
Author(s):  
Mahim Koshariya ◽  
Abhishek Shitole ◽  
Vibhore Agarwal ◽  
S. Dave
Keyword(s):  
Wound Healing ◽  
Secondary Infection ◽  
Healing Process ◽  
Gastrointestinal Diseases ◽  
Wound Healing Process ◽  
Burn Patients ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Group 2 ◽  
Group 1

Background: Sucralfate is a basic aluminum salt of sucrose octasulphate which was orally taken for prevention and treatment of several gastrointestinal diseases. This study primarily aims to analyze whether sucralfate accelerates wound healing process in burn patients. The incidence of infection & relieve in pain in burn patients was also compared.Methods: This is an observational study carried out in the Department of General Surgery, Hamidia Hospital Bhopal on 50 patients divided into group 1 (sucralfate)and group 2 [a-sucralfate; b-silver sulfadiazine (SSD)]. Demographics, history, physical, and systemic examinations of the patients were recorded.Results: It was observed that sucralfate augments the formation of granulation tissue (in 6-17 days) as compared to SSD (14-22 days). It was noticed that faster healing by re-epithelialization was present in sucralfate group (11-22 days) as compared with SSD group (15-30 days). By the end of 3rd week 50-75% of wound was healed in sucralfate group as compared with 35-50% in SSD group. Incidence of secondary infection was less when topical sucralfate was used (group 1 = 25%; group 2a = 16.6%; group 2b = 66.66%). There was a marked relief in pain and discomfort after sucralfate application as compared to SSD.Conclusions: Using topical sucralfate expedite the burn wound healing process, significantly decreases pain with no local or systemic adverse reactions to the topical application therefore it can be used as an adjunctive or alternative agent in the future. However, multicentric trials with larger sample size are needed to insure the concept.

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