scholarly journals CoNiZn and CoNiFe Nanoparticles: Synthesis, Physical Characterization, and In Vitro Cytotoxicity Evaluations

2021 ◽  
Vol 11 (12) ◽  
pp. 5339
Author(s):  
Sima Alikhanzadeh-Arani ◽  
Mohammad Almasi-Kashi ◽  
Saman Sargazi ◽  
Abbas Rahdar ◽  
Rabia Arshad ◽  
...  
Keyword(s):  
High Performance ◽  
Morphological Changes ◽  
Magnetic Measurement ◽  
In Vitro Cytotoxicity ◽  
Magnetic Characteristics ◽  
Ferromagnetic Behavior ◽  
Normal Human Cell ◽  
Normal Human ◽  
Vibration Sample Magnetometry

The polyol method has been used to synthesize CoNiFe and CoNiZn alloy nanoparticles (NPs). The magnetic characteristics of the products have been measured by vibration sample magnetometry (VSM) analysis. At the same time, the microstructure and morphology were inspected by X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. Magnetic measurement of samples by the VSM indicated that samples have soft ferromagnetic behavior. Spherical-shaped grains for samples were confirmed by the SEM. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and lactate dehydrogenase (LDH) assays were used to determine the cytotoxic effects of the synthesized NPs. Cytotoxic evaluations showed that treatment with 25 to 400 µg/mL of CoNiZn and CoNiFe NPs exerted a significant time- and concentration-dependent toxicity in MCF7 and HUVEC cells and markedly enhanced the LDH leakage after 48 h of exposure (p < 0.05 compared with untreated cells). Furthermore, NPs with concentrations higher than 12.5 µg/mL induced evident morphological changes in the studied cell lines. Treatment with 12.5 µg/mL of CoNiZn and CoNiFe NPs was safe and did not affect normal human cell survival. The results of in vitro cytotoxicity assessments show promise in supporting the suitability of the synthesized NPs to build high-performance theranostic nanoplatforms for simultaneous cancer imaging and therapy without affecting normal human cells.

Chemical carcinogen alteration of SV40 virus induced transformation of normal human cell populations in vitro

1978 ◽  
Vol 22 (2-3) ◽  
pp. 185-197 ◽  
Author(s):  
George E. Milo ◽  
James R. Blakeslee ◽  
Ronald Hart ◽  
David S. Yohn
Keyword(s):  
Human Cell ◽  
Cell Populations ◽  
Chemical Carcinogen ◽  
Sv40 Virus ◽  
Normal Human Cell ◽  
Normal Human

Matrix metalloproteinase-2 inhibition activity of Plukenetia volubilis L. leaves extract for anti-aging application

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 120-126
Author(s):  
N. Wuttisin ◽  
T. Nararatwanchai ◽  
A. Sarikaphuti
Keyword(s):  
High Performance ◽  
Antioxidant Property ◽  
In Vitro Cytotoxicity ◽  
Hot Water ◽  
Matrix Metalloproteinase 2 ◽  
Human Skin Fibroblast ◽  
Inhibition Activity ◽  
Hot Air ◽  
Plukenetia Volubilis

Plukenetia volubilis L. leaves were part of the traditional diets in many countries. P. volubilis leaves were used to make tea and sold as local products in Thailand. There is less information on the composition of P. volubilis leaves. Previous study revealed that roasted leaves extract with hot water showed the highest antioxidant activity and the antioxidant property might be due to the presence of flavonoid. The present study was carried out to determine the quercetin content in P. volubilis leaves extract and evaluate the anti-aging potential activities including MMP-2 inhibition activity and telomerase stimulation activity. P. volubilis leaves were roasted in hot air oven and extracted with hot water. The extract was investigated for quercetin content by high-performance liquid chromatography (HPLC). In vitro cytotoxicity, MMP-2 inhibition activity and telomerase stimulation activity were determined for anti-aging properties. The results revealed that P. volubilis leaves contained quercetin 50.50±4.78 mg/g DW. The extract showed no cytotoxicity on human skin fibroblast with cell viability of 96.76-120.83%. It demonstrated the potential of MMP-2 inhibition (8.74±2.84%) activity but lower than ascorbic acid. P. volubilis leave extract did not have telomerase stimulation activity on the human Hela cell line. However, the results from this study have indicated the possibility of anti-aging potential of P. volubilis leaves extract.

scholarly journals Antithymocyte globulin reacts with many normal human cell types

Blood ◽  
1983 ◽  
Vol 62 (5) ◽  
pp. 1047-1054 ◽  
Author(s):  
B Greco ◽  
L Bielory ◽  
D Stephany ◽  
SM Hsu ◽  
P Gascon ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Antithymocyte Globulin ◽  
Bone Marrow Cells ◽  
Cell Types ◽  
In Vitro Cytotoxicity ◽  
Cell Type ◽  
Circulating Lymphocytes ◽  
Flow Microfluorometry ◽  
Normal Human Cell

Abstract Antithymocyte globulin (ATG) is frequently effective therapy for aplastic anemia. Its mechanism of action is often assumed to be upon a lymphocyte inhibitor of hematopoiesis. However, specificity for T lymphocytes would not be anticipated from consideration of the method of preparing ATG. In fact, using flow microfluorometry and fluorescence immunohistochemistry, we have found that ATG binds to virtually all circulating lymphocytes, granulocytes, and platelets, as well as to bone marrow cells. Extensive absorption of ATG with either granulocytes or lymphocytes does not eliminate reactivity with the opposite cells, indicating that ATG recognizes some distinct antigens on each cell type. Treatment of cells with ATG blocks the binding of monoclonal antibodies directed against either lymphocyte differentiation or histocompatibility antigens. ATG also binds to visceral tissues, including thymus and testis cell membranes and the nuclear and cytoplasmic components of tonsil, kidney, liver, breast, lung, and intestine. In vitro cytotoxicity of ATG was demonstrated for both T and non- T lymphocytes and platelets. Despite its name, ATG is not specific for a particular cell type, and it would be premature to conclude that its effect is mediated through a specific lymphocyte population.

scholarly journals Assessment of environmental condition and drying process of the plants on the concentration of alkaloids and cytotoxicity of traditional Ayahuasca Tea

2021 ◽  
Vol 10 (2) ◽  
pp. 075-089
Author(s):  
Miranda Ordilena Ferreira de ◽  
Almeida Marcílio de ◽  
Sousa Ilza Maria de Oliveira ◽  
Ruiz Ana Lúcia Tasca Gois ◽  
Costa José Luiz da ◽  
...  
Keyword(s):  
High Performance ◽  
Colorimetric Assay ◽  
Human Keratinocytes ◽  
In Vitro Cytotoxicity ◽  
Drying Method ◽  
Drying Process ◽  
Forced Circulation ◽  
Different Temperatures ◽  
Array Detection

Introduction: Ayahuasca is a traditional psychoactive tea of Amazonian indigenous, used medicinal and spiritual purposes. Wide variation in the concentration of N,N-dimethyltryptamine (DMT), Harmaline (HRL), Harmine (HRM) and Tetrahydroharmine (THH) alkaloids in Ayahuasca has been reported worldwide. Objective: To evaluate the causes of variations in alkaloids concentrations of Ayahuasca prepared with fresh and dehydrated plants from different environments and determine the best drying method to plants according to alkaloids content and cytotoxicity of Ayahuasca tea. Material and methods: The environment interference on the alkaloids of the two species was evaluated in samples of Ayahuasca tea prepared with fresh plants. The most suitable drying process to the two species was evaluated in sample Ayahuasca tea prepared with plants submitted to drying under the sun conditions and five different temperatures in forced circulation oven. The concentration of the alkaloids determined by high performance liquid chromatography with UV-vis detector with diode array detection (HPLC-DAD). The in vitro cytotoxicity of Ayahuasca was evaluated in human keratinocytes cells (HaCaT) by colorimetric assay. Results: Environmental characteristics, preparation process and temperature of plants drying interfered on DMT, HRL, HRM and THH concentrations of Ayahuasca. No effect cytotoxicity was detected with relationship to psychoactive alkaloids in samples of Ayahuasca tea prepared with fresh or dried plants. Conclusion: Concentration of DMT, HRL, HRM and THH alkaloids in Ayahuasca are influenced by plants environmental. The most suitable drying process was obtained in forced circulation oven at 43 and 45°C to P. viridis leaves and B. caapi stems respectively. The Ayahuasca prepared with fresh or dry plants no showed cytotoxicity in human keratinocytes cells.

scholarly journals L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

2009 ◽  
Vol 20 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Rosana Belchior Miranda ◽  
Sandra Rivera Fidel ◽  
Maria Aparecida Affonso Boller
Keyword(s):  
Morphological Changes ◽  
Mineral Trioxide Aggregate ◽  
L929 Cell ◽  
Neutral Red ◽  
In Vitro Cytotoxicity ◽  
Mean Values ◽  
Nonviable Cell ◽  
Red Dye ◽  
Positive Controls

This study compared the cytotoxicity of an experimental epoxy-resin and calcium hydroxide-based cement (MBPc), gray mineral trioxide aggregate (MTA) and white mineral trioxide aggregate (WMTA) using the agar overlay method with neutral red dye. L929 cells were seeded into 6-well culture plates where 48-h set test materials were placed on the agar overlay, in triplicate. Teflon and natural rubber served as negative and positive controls. After an incubation period of 24 h at 37ºC in a humidified atmosphere of 5% CO2 in air, a discolored area around the samples and the positive controls could be observed and measured per quadrant. The mean values were compared and converted into grades to classify the results according to the table of cytotoxicity grades according to the Standard Operating Procedures (SOP) of the Oswaldo Cruz Foundation, Brazil. The nonviable cell areas and the morphological changes in the cells were observed with an inverted microscope. The results showed grade 1 (slight) for the two types of MTA (p>0.05) and grade 2 (mild) for the MBPc (p<0.001). All samples met the requirements of the test as none of the cultures showed reactivity higher than grade 2.

Fate of Ochratoxin A during Cooking of Naturally Contaminated Polished Rice

2005 ◽  
Vol 68 (10) ◽  
pp. 2107-2111 ◽  
Author(s):  
JE WON PARK ◽  
SOO-HYUN CHUNG ◽  
CHAN LEE ◽  
YOUNG-BAE KIM
Keyword(s):  
Ochratoxin A ◽  
High Performance ◽  
Glioma Cells ◽  
In Vitro Cytotoxicity ◽  
C6 Glioma Cells ◽  
Cytotoxic Potential ◽  
Cooked Rice ◽  
Polished Rice ◽  
Low Levels

Ochratoxin A (OTA), a mycotoxin widespread in cereals, occurs in polished rice that is consumed as cooked rice after washing and steaming. Cooking decreases OTA levels in food to varying extents, but little is known about how cooking changes the biological activity of this mycotoxin. We therefore evaluated the fate of OTA during rice cooking to determine the OTA residues and cytotoxic potential in vitro. Water-washed rice, ordinary cooked rice, and pressure-cooked rice were prepared from three polished rice lots naturally contaminated with OTA. Residual OTA in each sample was analyzed by high-performance liquid chromatography (HPLC), whereas in vitro cytotoxicity of OTA to C6 glioma cells, susceptible to low levels (nanograms per milliliter) of OTA, was used to confirm the chemical analysis. OTA concentration, as determined by HPLC analysis, in the cooked rice by both types of cookers was significantly lower than (59 to 75%) in the raw polished rice and water-washed rice. The cytotoxicity of the OTA that remained in the pressure-cooked rice from three lots was markedly decreased (approximately 20%, P &lt; 0.05) when compared with other samples in respective lots. This confirms that cooking lowers OTA residues. Although washing polished rice with water had little effect on OTA levels, pressure steaming appeared to be the critical cooking step not only to reduce OTA residues in polished rice before reaching the consumer as the dietary staple of cooked rice, but also to diminish cytotoxicity of OTA.

scholarly journals EVALUATION OF ANTI-CML ACTIVITY OF METHANOL AND AQUEOUS EXTRACTS OF BENKARA MALABARICA (LAM.) TIRVENG PLANT LEAVES

2018 ◽  
Vol 10 (5) ◽  
pp. 112
Author(s):  
Kalubai Vari Khajapeer ◽  
Ranjan Biswal ◽  
Rajasekaran Baskaran
Keyword(s):  
Cell Cycle ◽  
Morphological Changes ◽  
K562 Cells ◽  
In Vitro Cytotoxicity ◽  
Gas Chromatography Mass Spectrometry ◽  
Plant Leaves ◽  
Dapi Staining ◽  
Cycle Arrest ◽  
Diphenyltetrazolium Bromide

Objective: To investigate the phytoconstituents and in vitro cytotoxicity of methanol (MeOH) and aqueous (AQE) extracts of Benkara malabarica (Lam.) Triveng (BM) plant leaves.Methods: Gas chromatography-mass spectrometry (GC MS) was carried out to disclose the principal phytoconstituents present in MeOH and AQE extracts of BM. In vitro cytotoxicity of BM extracts were determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Acridine orange (AO)/ethidium bromide (EB) and 4', 6-diamidino-2-phenylindole (DAPI) staining were performed to visualize morphological changes upon treatment of BM extracts. Fluorescence-activated cell sorting (FACS) was carried out to determine the apoptosis and cell cycle arrestability of BM extracts.Results: GC MS analysis reported the presence of nine phytoconstituents in MeOH and AQE extracts of BM. The IC50 of BM MeOH, AQE extracts treated K562 cells were 49.78±1.697, 15.47±1.19 µg/ml for 48 h and found to be statistically significant (p<0.001). AO/EB and DAPI staining results anticipated the induction of apoptosis and DNA fragmentation upon treatment of BM extracts. FACS analysis revealed the SubG0 cell populations increased in K562 cells treated by BM MeOH (18.15) and AQE (51.26) extracts.Conclusion: The results of the present study uncovered that the BM AQE extract was more potent in inhibiting K562 cell proliferation through cell cycle arrest and apoptosis compared to the MeOH extract of BM. 

Synthesis, Structure, and Selective Cytotoxicity of Organometallic Cp*RuII O-Alkyl-N-phenylcarbamate Sandwich Complexes

2010 ◽  
Vol 63 (2) ◽  
pp. 245 ◽  
Author(s):  
Bradley T. Loughrey ◽  
Michael L. Williams ◽  
Thomas J. Carruthers ◽  
Peter G. Parsons ◽  
Peter C. Healy
Keyword(s):  
Fibroblast Cell ◽  
In Vitro Cytotoxicity ◽  
Sandwich Complexes ◽  
One Pot ◽  
Cytotoxicity Studies ◽  
Structure Determinations ◽  
Normal Human ◽  
Fourier Transform Ir ◽  
Potent Growth

Tetraphenylborate salts of the η6-arene Cp*RuII O-alkyl-N-phenyl carbamate organometallic sandwich complexes, [Cp*Ru(PhNHCO2R)]BPh4 for R = Me (1), Et (2), and n-Pr (3), have been prepared by a facile one-pot reaction between ruthenium trichloride, pentamethylcyclopentadiene, and phenylisocyanate in refluxing alcohol solutions, and have been characterized by Fourier-transform IR and NMR spectroscopy, electrospray mass spectrometry, and single-crystal X-ray structure determinations. In vitro cytotoxicity studies show the complexes to be potent growth inhibitors for a range of tumour cell lines, while expressing significantly lower levels of toxicity towards a normal human fibroblast cell line.

Suramin affects differentiated and undifferentiated human thyroid epithelial cells in vitro

1992 ◽  
Vol 134 (3) ◽  
pp. 505-NP ◽  
Author(s):  
K. Trieb ◽  
K. Dorfinger ◽  
N. Neuhold ◽  
E. Selzer ◽  
A. Wilfing ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Serum Levels ◽  
Surface Expression ◽  
Human Thyroid ◽  
Thyroid Cells ◽  
Thyroid Carcinomas ◽  
Hla Dr ◽  
Normal Human ◽  
In Vitro Effects

ABSTRACT In the last decade, suramin has become known for its antiproliferative, differentiation-inducing effects on cells and has been successfully used in the therapy of cancer patients. The present study was undertaken to investigate the effects of suramin on normal human thyroid cells in primary monolayer culture and to analyse whether it also affected cells from thyroid carcinomas. The results show that suramin, at concentrations similar to serum levels obtainable during therapy, inhibited the proliferation of thyroid cells as well as the secretion of thyroglobulin. It suppressed the activation of adenylyl cyclase in thyroid membranes and decreased the immunogenicity of the cells by reducing their surface expression of HLA-DR and ICAM-1. Although the morphology of differentiated thyroid cells remained unaffected by suramin, morphological changes compatible with differentiation were observed in cells from undifferentiated thyroid carcinomas when suramin was added to the culture medium. In conclusion, the data demonstrate that suramin has pronounced in-vitro effects on normal and neoplastic thyroid cells. It may, therefore, also be effective in patients with thyroid cancer, for whom no other form of treatment is available. Journal of Endocrinology (1992) 134, 505–511

Sign in / Sign up

Export Citation Format

Share Document