scholarly journals Extracellular Matrix Behaviour in Masseter Muscle of Patients Affected by Unilateral Posterior Crossbite: An Immunofluorescence Study

2021 ◽  
Vol 11 (10) ◽  
pp. 4649
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Maria Grazia Piancino ◽  
Maria Chiara Malandrino ◽  
Michele Runci Anastasi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Masseter Muscle ◽  
Muscle Hypertrophy ◽  
Contralateral Side ◽  
Collagen Iv ◽  
Hypertrophic Response ◽  
Posterior Crossbite ◽  
Increased Activity ◽  
Reduced Activity ◽  
Contralateral Muscle

Unilateral posterior crossbite is a malocclusion disease that involves morpho-functional characteristics of masseter muscle; a normal or increased activity of contralateral muscle and a reduced activity of the ipsilateral muscle during unilateral crossbite have been shown. Since the extracellular matrix plays a key role in in mechano-transduction of transmitting forces during muscle contraction, the aim of the present study was to analyse the behaviour of extracellular matrix in this type of malocclusion through immunofluorescence reactions against laminin, collagen IV, MMP-2 and MMP-9. Our results show an increased expression of Laminin, Collagen IV, and MMP-9 in the contralateral side if compared to the ipsilateral side. No differences have been found in MMP-2 expression between contralateral and ipsilateral muscles. Since the increased expression of Laminin, Collagen IV and MMP-9 is associated with muscle hypertrophy and MMP-2 is associated with muscle atrophy, our results support the existence of a hypertrophic response of contralateral muscle during unilateral posterior crossbite that probably aims to compensate the altered function of the ipsilateral one.

scholarly journals Immunofluorescence Evaluation of Myf5 and MyoD in Masseter Muscle of Unilateral Posterior Crossbite Patients

2020 ◽  
Vol 5 (4) ◽  
pp. 80
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Guglielmo Ramieri ◽  
Michele Tepedino ◽  
Michele Runci Anastasi ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Masseter Muscle ◽  
Muscle Hypertrophy ◽  
Contralateral Side ◽  
Surgical Patients ◽  
Posterior Crossbite ◽  
Fibre Formation ◽  
Masseter Muscles ◽  
Low Activity ◽  
Contralateral Muscle

A unilateral posterior crossbite is a malocclusion where the low activity of the affected masseter muscle is compensated by the contralateral muscle hypertrophy. It is still unknown if, in the same condition, myogenesis with new fibre formation takes place. Aim: the aim of the present study was to evaluate the expression of myogenesis markers, such as Myf5 and MyoD, in masseter muscles of unilateral posterior crossbite patients. Materials and methods: biopsies from fifteen surgical patients with unilateral posterior crossbites have been analysed by immunofluorescence reactions. The results show the expression of Myf5 and MyoD in the contralateral muscle but not in the ipsilateral one. Moreover, statistical analysis shows the higher number of satellite cells in the contralateral side if compared to the ipsilateral one. Conclusions: these results suggest that in contralateral muscle, hyperplastic events take place, as well as hypertrophy.

scholarly journals Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study

2021 ◽  
Vol 11 (12) ◽  
pp. 5350
Author(s):  
Giovanna Vermiglio ◽  
Mariagrazia Piancino ◽  
Michele Runci Anastasi ◽  
Giacomo Picciolo ◽  
Antonio Centofanti ◽  
...  
Keyword(s):  
Masseter Muscle ◽  
Muscle Fibers ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Posterior Crossbite ◽  
Affected Side ◽  
The Cross ◽  
And Control ◽  
Contralateral Muscle

Unilateral posterior crossbite is a type of malocclusion that involves morpho-functional characteristics of masticatory muscle, such as the masseter: electrophysiological data have shown that the affected side works less than the contralateral muscle, which shows a normal or increased activity, probably in order to compensate for the affected side. The aim of present work was to measure the diameter and the cross-sectional area of ipsilateral and contralateral muscle fibers to verify if hypertrophy and/or hypotrophy take place in this malocclusion. We used immunofluorescence pictures to measure, using ImageJ software, the diameter and the cross-sectional area of fibers from control and crossbite groups; after that, the data were processed to perform statistical analyses. Results show that the fiber diameters of contralateral muscle are larger than the diameters of ipsilateral and control fibers, and that this difference is statistically significant. No statistically significant difference was found between the fiber diameters of the ipsilateral and control muscles. All these data suggest that, during unilateral posterior crossbite, morphological changes take place in the contralateral masseter muscle, which undergoes hypertrophy, probably to compensate for the low activity of the affected muscle.

scholarly journals Morphofunctional compensation of masseter muscles in unilateral posterior crossbite patients

2016 ◽  
Vol 60 (2) ◽  
Author(s):  
G. Cutroneo ◽  
G. Vermiglio ◽  
A. Centofanti ◽  
G. Rizzo ◽  
M. Runci ◽  
...  
Keyword(s):  
Masticatory Muscles ◽  
Contralateral Side ◽  
Dental Arch ◽  
Ipsilateral Side ◽  
Hypertrophic Response ◽  
Posterior Crossbite ◽  
And Function ◽  
Relationship Of ◽  
Masseter Muscles

Unilateral posterior crossbite is a widespread, asymmetric malocclusion characterized by an inverse relationship of the upper and lower buccal dental cusps, in the molar and premolar regions, on one side only of the dental arch. Patients with unilateral posterior crossbite exhibit an altered chewing cycles and the crossbite side masseter results to be less active with respect to the contralateral one. Few studies about morphological features of masticatory muscle in malocclusion disorders exist and most of these have been performed on animal models. The aim of the present study was to evaluate morphological and protein expression characteristics of masseter muscles in patients affected by unilateral posterior crossbite, by histological and immunofluorescence techniques. We have used antibody against PAX-7, marker of satellite cells, and against α-, β-, γ-, δ-, ε- and ζ-sarcoglycans which are transmembrane glycoproteins involved in sarcolemma stabilization. By statistical analysis we have evaluated differences in amount of myonucley between contralateral and ipsilateral side. Results have shown: i) altered fibers morphology and atrophy of ipsilateral muscle if compared to the contralateral one; ii) higher number of myonuclei and PAX-7 positive cells in contralateral side than ipsilateral one; iii) higher pattern of fluorescence for all tested sarcoglycans in contralateral side than ipsilateral one. Results show that in unilateral posterior crossbite hypertrophic response of contralateral masseter and atrophic events in ipsilateral masseter take place; by that, in unilateral posterior crossbite malocclusion masticatory muscles modify their morphology depending on the function. That could be relevant in understanding and healing of malocclusion disorders; in fact, the altered balance about structure and function between ipsilateral and contralateral muscles could, long-term, lead and/ or worsen skeletal asymmetries.

Cardiac myocytes cultured on a basement membrane extract of the ehs tumor

1986 ◽  
Vol 44 ◽  
pp. 270-271
Author(s):  
L. Terracio ◽  
A. Dewey ◽  
K. Rubin ◽  
T.K. Borg
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Cardiac Myocytes ◽  
Normal Tissue ◽  
Cell Spreading ◽  
Collagen Iv ◽  
Basement Membrane Extract ◽  
Membrane Extract ◽  
Growth Development ◽  
Multicellular Organisms

The recognition and interaction of cells with the extracellular matrix (ECM) effects the normal physiology as well as the pathology of all multicellular organisms. These interactions have been shown to influence the growth, development, and maintenance of normal tissue function. In previous studies, we have shown that neonatal cardiac myocytes specifically interacts with a variety of ECM components including fibronectin, laminin, and collagens I, III and IV. Culturing neonatal myocytes on laminin and collagen IV induces an increased rate of both cell spreading and sarcomerogenesis.

Juvenile hormone signaling promotes ovulation and maintains egg shape by inducing expression of extracellular matrix genes

2021 ◽  
Vol 118 (39) ◽  
pp. e2104461118
Author(s):  
Wei Luo ◽  
Suning Liu ◽  
Wenqiang Zhang ◽  
Liu Yang ◽  
Jianhua Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Muscle Contraction ◽  
Juvenile Hormone ◽  
Fat Body ◽  
Collagen Iv ◽  
Hormone Signaling ◽  
Gonadotropic Hormone ◽  
Egg Shape ◽  
Insect Reproduction

It is well documented that the juvenile hormone (JH) can function as a gonadotropic hormone that stimulates vitellogenesis by activating the production and uptake of vitellogenin in insects. Here, we describe a phenotype associated with mutations in the Drosophila JH receptor genes, Met and Gce: the accumulation of mature eggs with reduced egg length in the ovary. JH signaling is mainly activated in ovarian muscle cells and induces laminin gene expression in these cells. Meanwhile, JH signaling induces collagen IV gene expression in the adult fat body, from which collagen IV is secreted and deposited onto the ovarian muscles. Laminin locally and collagen IV remotely contribute to the assembly of ovarian muscle extracellular matrix (ECM); moreover, the ECM components are indispensable for ovarian muscle contraction. Furthermore, ovarian muscle contraction externally generates a mechanical force to promote ovulation and maintain egg shape. This work reveals an important mechanism for JH-regulated insect reproduction.

Hormone Balance and the Control of Metamorphosis in Rhodnius Prolixus (Hemiptera)

1952 ◽  
Vol 29 (4) ◽  
pp. 620-631
Author(s):  
V. B. WIGGLESWORTH
Keyword(s):  
Juvenile Hormone ◽  
Corpus Allatum ◽  
Corpora Allata ◽  
Stage Larva ◽  
Mature Adults ◽  
Hormone Balance ◽  
Low Concentrations ◽  
Moulting Cycle ◽  
Increased Activity ◽  
Reduced Activity

A technique is described by which the intact larva of Rhodnius can be transfused with blood from another larva without interfering with ecdysis. If the 4th-stage larva receives blood from a 3rd-stage larva it develops characters little different from those of the 4th instar. This is attributed to the 3rd-stage larva producing juvenile hormone at a higher concentration. If the 4th-stage larva at 24 hr. after feeding receives blood from another 4th-stage larva at 8 days after feeding it develops characters intermediate between those of the 4th and 5th instars. This is attributed to the juvenile hormone being introduced too early in the moulting cycle. The hormone balance is upset by abnormal temperatures. The 4th-stage larva will not moult at a temperature of 36° C. although the larvae can survive up to about 40° C. At temperatures a little below 36° C. moulting is somewhat delayed and the characters developed are slightly ‘adultoid’ (prothetely). This is attributed to slightly reduced activity of the corpus allatum. At temperatures below 20° C. moulting is greatly delayed and the characters developed are slightly ‘juvenile’ (metathetely). This is attributed to relatively increased activity of the corpus allatum. Low concentrations of oxygen (less than 5 %) have an effect similar to that of high temperature. If 5th-stage larvae of Rhodnius receive implants of corpora allata from mature adults of Periplaneta they develop into 6th-stage larvae and many of these subsequently into 7th-stage larvae. The ‘juvenile hormone’ appears to be the same in the two insects. No evidence could be obtained for the persistence of juvenile hormone in the blood from one instar of Rhodnius to the next. The hypothesis of an active elimination of juvenile hormone by the corpus allatum at the time of metamorphosis remains therefore unproven.

scholarly journals Bioengineered airway epithelial grafts with mucociliary function based on collagen IV- and laminin-containing extracellular matrix scaffolds

2020 ◽  
Vol 55 (6) ◽  
pp. 1901200 ◽  
Author(s):  
Nick J.I. Hamilton ◽  
Dani Do Hyang Lee ◽  
Kate H.C. Gowers ◽  
Colin R. Butler ◽  
Elizabeth F. Maughan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Airway Epithelium ◽  
Airway Epithelial Cell ◽  
Collagen Iv ◽  
Airway Epithelial ◽  
Cell Adherence ◽  
Bronchial Epithelial ◽  
Mucociliary Function

Current methods to replace damaged upper airway epithelium with exogenous cells are limited. Existing strategies use grafts that lack mucociliary function, leading to infection and the retention of secretions and keratin debris. Strategies that regenerate airway epithelium with mucociliary function are clearly desirable and would enable new treatments for complex airway disease.Here, we investigated the influence of the extracellular matrix (ECM) on airway epithelial cell adherence, proliferation and mucociliary function in the context of bioengineered mucosal grafts. In vitro, primary human bronchial epithelial cells (HBECs) adhered most readily to collagen IV. Biological, biomimetic and synthetic scaffolds were compared in terms of their ECM protein content and airway epithelial cell adherence.Collagen IV and laminin were preserved on the surface of decellularised dermis and epithelial cell attachment to decellularised dermis was greater than to the biomimetic or synthetic alternatives tested. Blocking epithelial integrin α2 led to decreased adherence to collagen IV and to decellularised dermis scaffolds. At air–liquid interface (ALI), bronchial epithelial cells cultured on decellularised dermis scaffolds formed a differentiated respiratory epithelium with mucociliary function. Using in vivo chick chorioallantoic membrane (CAM), rabbit airway and immunocompromised mouse models, we showed short-term preservation of the cell layer following transplantation.Our results demonstrate the feasibility of generating HBEC grafts on clinically applicable decellularised dermis scaffolds and identify matrix proteins and integrins important for this process. The long-term survivability of pre-differentiated epithelia and the relative merits of this approach against transplanting basal cells should be assessed further in pre-clinical airway transplantation models.

Tolbutamide inhibits glucagon-induced phosphorylation of 6PF-2-K/Fru-2,6-P2ase in rat hepatocytes

1995 ◽  
Vol 268 (3) ◽  
pp. E391-E396
Author(s):  
H. Ayame ◽  
A. Matsutani ◽  
H. Inoue ◽  
T. Kaneko ◽  
K. Kaku
Keyword(s):  
Rat Hepatocytes ◽  
Bifunctional Enzyme ◽  
Dependent Manner ◽  
Enzyme Protein ◽  
Dependent Protein ◽  
Sulfonylurea Drugs ◽  
Dose Dependent ◽  
Increased Activity ◽  
Reduced Activity

In previous studies, we demonstrated that tolbutamide inhibits a phosphorylation of hepatic 6-phosphofructo-2-kinase (6PF-2-K)/fructose-2,6-bisphosphatase (Fru-2,6-P2ase) catalyzed by the adenosine 3',5'-cyclic monophosphate-dependent protein kinase in a reconstruction system using the purified enzyme from the rat liver. In the current study, to assess a role of tolbutamide on hepatic 6PF-2-K/Fru-2,6-P2ase physiologically, we used intact rat hepatocytes and examined effects of tolbutamide on a phosphorylation of the bifunctional enzyme in the presence of glucagon. Glucagon induced a rapid phosphorylation of hepatic 6PF-2-K/Fru-2,6-P2ase accompanied by an inhibition of 6PF-2-K activity and a stimulation of Fru-2,6-P2ase activity in a dose-dependent manner. Tolbutamide inhibited glucagon-induced phosphorylation of the bifunctional enzyme protein in a dose-dependent manner. By adding 2 mM tolbutamide, reduced activity of 6PF-2-K and increased activity of Fru-2,6-P2ase in the presence of 10(-9) M glucagon were partially restored. The present results suggest the possibility that tolbutamide modulates the activity of hepatic 6PF-2-K/Fru-2,6-P2ase through inhibiting a phosphorylation of the enzyme protein. The counterregulatory influence of tolbutamide on the effect of glucagon suggests a possible mechanism for the extrapancreatic effect of sulfonylurea drugs.

Hyperoxia increases plasminogen activator activity of cultured endothelial cells

1992 ◽  
Vol 262 (1) ◽  
pp. L21-L31 ◽  
Author(s):  
P. G. Phillips ◽  
L. Birnby ◽  
L. A. Di Bernardo ◽  
T. J. Ryan ◽  
M. F. Tsan
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Collagen Iv ◽  
Matrix Proteins ◽  
Time Dependent ◽  
Focal Contact ◽  
Oxygen Exposure ◽  
Endothelial Monolayers ◽  
Cell Fractions

Confluent calf pulmonary artery endothelial monolayers exposed to 95% oxygen for 1, 2, or 3 days exhibit a time-dependent increase in adherence to substratum, which closely parallels changes in actin cytoarchitecture and the distribution of focal contact proteins vinculin and talin. Oxygen exposure also resulted in elevated plasminogen activator (PA) activity in conditioned media (CM) and in cytoskeletal protein- and focal contact protein-enriched fractions, with highest levels achieved in the latter two fractions at 48 h after oxygen exposure. PAs have been shown to participate in dismantling of extracellular matrix in a number of physiological and pathological situations. Immunocytochemical studies demonstrated extensive restructuring of matrix proteins collagen IV, laminin, and fibronectin, which correlated temporally with elevated PA levels. Further, when protease-containing cell fractions were used to study degradation of isolated matrices, those obtained from hyperoxia-exposed cells were substantially more active than those from normoxia-exposed cells. Our data suggest that hyperoxia-induced production of PA (and perhaps other proteases) may be partly responsible for degradation of the extracellular matrix of endothelial cells.

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