scholarly journals Human Laryngeal Mucus from the Vocal Folds: Rheological Characterization by Particle Tracking Microrheology and Oscillatory Shear Rheology

2021 ◽  
Vol 11 (7) ◽  
pp. 3011
Author(s):  
Gregor Peters ◽  
Olaf Wendler ◽  
David Böhringer ◽  
Antoniu-Oreste Gostian ◽  
Sarina K. Müller ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Network Formation ◽  
Ex Vivo ◽  
Demographic Data ◽  
Smoking Behavior ◽  
Vocal Folds ◽  
Oscillatory Shear ◽  
Rheological Characterization ◽  
Shear Rheology ◽  
Particle Tracking Microrheology

Mucus consistency affects voice physiology and is connected to voice disorders. Nevertheless, the rheological characteristics of human laryngeal mucus from the vocal folds remain unknown. Knowledge about mucus viscoelasticity enables fabrication of artificial mucus with natural properties, more realistic ex-vivo experiments and promotes a better understanding and improved treatment of dysphonia with regard to mucus consistency. We studied human laryngeal mucus samples from the vocal folds with two complementary approaches: 19 samples were successfully applied to particle tracking microrheology (PTM) and five additional samples to oscillatory shear rheology (OSR). Mucus was collected by experienced laryngologists from patients together with demographic data. The analysis of the viscoelasticity revealed diversity among the investigated mucus samples according to their rigidity (absolute G’ and G”). Moreover some samples revealed throughout solid-like character (G’ > G”), whereas some underwent a change from solid-like to liquid-like (G’ < G”). This led to a subdivision into three groups. We assume that the reason for the differences is a variation in the hydration level of the mucus, which affects the mucin concentration and network formation factors of the mucin mesh. The demographic data could not be correlated to the differences, except for the smoking behavior. Mucus of predominant liquid-like character was associated with current smokers.

Lubricin Interaction With Hyaluronate Promotes Interchain Association Leading to Network Formation

2005 ◽  
Author(s):  
J. R. Torres ◽  
G. D. Jay ◽  
M. L. Warman ◽  
R. M. Laxer ◽  
M. Laderer ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Synovial Fluid ◽  
Particle Tracking ◽  
Network Formation ◽  
Molecular Layering ◽  
Cartilage Surface ◽  
Particle Tracking Microrheology ◽  
Boundary Lubricant ◽  
Multiple Particle Tracking ◽  
Multiple Particle

The present work is the first instance where a novel multiple-particle tracking microrheology technique has been applied to study molecular interactions of clinical significance. Herein we describe the molecular changes due to lubricin-hyaluronate interaction and their effect on mechanical properties of synovial fluid. Along with bulk rheology studies it was found that lubricin, a boundary lubricant, increases the HA network formation conducive to the enhanced molecular layering of HA under stress which results in increased shear thinning. This interaction may also allow HA molecules to bind to the cartilage surface, providing boundary lubrication, by virtue of its interaction with lubricin.

Multiple particle tracking

2018 ◽  
Author(s):  
Eric M. Furst ◽  
Todd M. Squires
Keyword(s):  
Brownian Motion ◽  
Best Practices ◽  
Particle Tracking ◽  
Heterogeneous Material ◽  
Quantitative Measurements ◽  
Particle Tracking Microrheology ◽  
Shell Models ◽  
Multiple Particle Tracking ◽  
Microscopy Data ◽  
Multiple Particle

The fundamentals and best practices of multiple particle tracking microrheology are discussed, including methods for producing video microscopy data, analyzing data to obtain mean-squared displacements and displacement correlations, and, critically, the accuracy and errors (static and dynamic) associated with particle tracking. Applications presented include two-point microrheology, methods for characterizing heterogeneous material rheology, and shell models of local (non-continuum) heterogeneity. Particle tracking has a long history. The earliest descriptions of Brownian motion relied on precise observations, and later quantitative measurements, using light microscopy.

scholarly journals Plate reader microrheology

2020 ◽  
Author(s):  
Robert F. Hawkins ◽  
Gregg A. Duncan
Keyword(s):  
Particle Tracking ◽  
Fluorescence Polarization ◽  
Rotational Diffusion ◽  
Polymeric Materials ◽  
Soft Materials ◽  
Clinical Samples ◽  
Clinical Settings ◽  
Particle Tracking Microrheology ◽  
Plate Reader ◽  
Potential Applications

AbstractIn this work, we report the development of a simplified microrheological method that can be used to rapidly study soft materials. This approach uses fluorescence polarization and a plate reader format to measure the rotational diffusion of nanoparticles within a sample of interest. We show that this measurement is sensitive to viscosity-dependent changes in polymeric soft materials and is correlated with particle tracking microrheology, a previously validated measure of microrheology. Using these fluorescence polarization-based measurements, we describe formalism that enables reasonable estimation of viscosity in polymeric materials after accounting for length-scale dependent effects of the polymer environment on the nanoparticle rotational diffusion. The use of a plate reader format allows this approach to be higher throughput, less technically challenging, and more widely accessible than standard macro- and microrheological methods, making it available to non-experts. This approach has potential applications in academic and industry settings where conventional rheological equipment may not be available, as well as in clinical settings to rapidly characterize human clinical samples.

scholarly journals Particle tracking microrheology of purified gastrointestinal mucins

Biopolymers ◽  
2014 ◽  
Vol 101 (4) ◽  
pp. 366-377 ◽  
Author(s):  
Pantelis Georgiades ◽  
Paul D. A. Pudney ◽  
David J. Thornton ◽  
Thomas A. Waigh
Keyword(s):  
Particle Tracking ◽  
Particle Tracking Microrheology

Particle tracking microrheology of cancer cells in living subjects

2020 ◽  
Vol 39 ◽  
pp. 98-109 ◽  
Author(s):  
Pei-Hsun Wu ◽  
Sanjiv Sam Gambhir ◽  
Christopher M. Hale ◽  
Wei-Chiang Chen ◽  
Denis Wirtz ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Particle Tracking ◽  
Particle Tracking Microrheology

Particle Tracking Microrheology of Lyotropic Liquid Crystals

Langmuir ◽  
2011 ◽  
Vol 27 (10) ◽  
pp. 6171-6178 ◽  
Author(s):  
Mohammad Mydul Alam ◽  
Raffaele Mezzenga
Keyword(s):  
Liquid Crystals ◽  
Particle Tracking ◽  
Lyotropic Liquid Crystals ◽  
Particle Tracking Microrheology
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