scholarly journals Edible Flowers Extracts as a Source of Bioactive Compounds with Antioxidant Properties—In Vitro Studies

2021 ◽  
Vol 11 (5) ◽  
pp. 2120
Author(s):  
Karolina Jakubczyk ◽  
Agnieszka Łukomska ◽  
Izabela Gutowska ◽  
Joanna Kochman ◽  
Joanna Janił ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Antioxidant Properties ◽  
Red Clover ◽  
Total Content ◽  
Antioxidant Potential ◽  
Antioxidant Power

Edible plants began to play an important role in past decade as a part of therapy, a recovery process or a healthy life style. The availability and relatively low price of the raw material, as well as proven bioactive health benefits, are key to consumers’ choice of nutrients. The red clover (Trifolium pratense) is a popular plant with healthy properties such as antiseptic and analgesic effects. The less known white clover (Trifolium repens), a fodder and honey plant, has anti-rheumatic and anti-diabetic properties. Both species may serve as a potential source of bioactive substances with antioxidant properties as a food additive or supplement. The study material consisted of flower extracts of Trifolium repens and Trifolium pratense. The total content of polyphenols and DPPH (2.2-diphenyl-1-picrylhydrazyl) and ferric reducing antioxidant power (FRAP) were measured using spectrophotometry methods. Oxidative stress in THP1 cells was induced via sodium fluoride. Subsequently, flower extracts were added and their influences on proliferation, antioxidant potential and the activity of antioxidant enzymes were evaluated. The extracts have a high total content of polyphenols as well as high antioxidant potential. We also demonstrated positive extracts impact on cells proliferation, high antioxidant potential and increasing activity of antioxidant enzymes on cell cultures under high oxidative stress induced by fluoride. Both red clover and the less known white clover may serve as valuable sources of antioxidants in the everyday diet.

scholarly journals Bioactive Compounds in Aegopodium podagraria Leaf Extracts and Their Effects against Fluoride-Modulated Oxidative Stress in the THP-1 Cell Line

2021 ◽  
Vol 14 (12) ◽  
pp. 1334
Author(s):  
Karolina Jakubczyk ◽  
Agnieszka Łukomska ◽  
Sylwester Czaplicki ◽  
Anna Wajs-Bonikowska ◽  
Izabela Gutowska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Essential Oil ◽  
Cell Line ◽  
Antioxidant Properties ◽  
Total Content ◽  
Antioxidant Potential ◽  
Leaf Extracts ◽  
Positive Effects

Aegopodium podagraria L. (goutweed), a member of the Apiaceae family, is a common perennial plant found all around the world that has been used in folk medicine since antiquity. Goutweed leaves contain polyacetylenes, essential oils, mono- and sesquiterpenes, vitamins, macro- and microelements, and phenolic compounds. In spite of its many health-promoting properties, including antioxidant effects, the plant has not been thoroughly studied. The aim of this study was to investigate the antioxidant properties of different goutweed leaf extracts and their effects on the THP-1 cell line, and also to describe the chemical characteristics of goutweed. Falcarinol and falcarindiol and essential oil were determined by gas chromatography coupled with mass spectrometry. Spectrophotometry was used to measure the total content of polyphenols and antioxidant activity–by DPPH and FRAP methods. Oxidative stress in THP-1 cells was induced via sodium fluoride. Then, goutweed leaf extracts were added to evaluate their influence on antioxidant potential (ABTS) and the activity of antioxidant enzymes. Confocal microscopy was used to visualise the production of cytoplasmic and mitochondrial reactive oxygen species (ROS) and for in vitro imaging of apoptosis. The ethanol extracts have a high total content of polyphenols, polyacetylenes, and essential oil, as well as high antioxidant potential. The main volatiles represented diverse chemical groups, which are both oxygenated derivatives of sesquiterpenes and monoterpenes. We also demonstrated positive effects of the high antioxidant potential and increased activity of antioxidant enzymes on cell cultures under severe fluoride-induced oxidative stress. Extraction at 80 ℃ and the use of ethanol as a solvent increased the antioxidant capacity of the extract. The leaves of Aegopodium podagraria may serve as a valuable source of antioxidants in the daily diet and assist in the prevention and treatment of oxidative stress-mediated conditions, e.g., inflammatory conditions, cardiovascular diseases, neurodegenerative diseases, and even obesity.

Selective behaviour in cattle grazing pastures of strips of birdsfoot trefoil and red clover. 2. The effects of sward maturity and structure

2006 ◽  
Vol 144 (2) ◽  
pp. 173-181 ◽  
Author(s):  
C. H. E. C. POLI ◽  
J. HODGSON ◽  
G. P. COSGROVE ◽  
G. C. ARNOLD
Keyword(s):  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Birdsfoot Trefoil ◽  
Grazing Cattle ◽  
Biting Rate ◽  
Partial Preference ◽  
Ingestive Behaviour ◽  
Selection Of

The effects of manipulation of sward maturity on ingestive behaviour and diet selection of grazing cattle were tested in two experiments using legumes which are equally preferred by cattle. Yearling heifers, in groups of three, grazed plots formed by alternate 2·4 m wide strips of a mixture of birdsfoot trefoil (Lotus corniculatus L.) and white clover (Trifolium repens L.) (BW) and strips of red clover (Trifolium pratense L.) (RC). The treatments comprised four combinations of each of the two swards at two contrasting levels of maturity/height replicated over four successive 3-day periods, in an order which balanced the effect of previous treatments. Observations of the distribution of grazing activity and biting rate were made over 3-hour periods each evening and in the morning of Day 2.Rate of biting was generally greater on immature than mature swards, and on BW than on RC, with little evidence of interactions between these effects. The animals demonstrated partial preference throughout the two studies, the proportion of grazing time devoted to BW being 0·40±0·010 in Expt 1 and 0·47±0·017 in Expt 2. The distribution of grazing time between sward type and maturity combinations was influenced progressively by herbage mass and height contrasts as strips were grazed down. An overall analysis using the results from these studies and a companion experiment indicated differences between studies in partial preference for BW and RC which appeared to be related to variations in sward morphology.

scholarly journals Ileal amino acid digestibilities in pigs of barley-based diets with inclusion of lucerne (Medicago sativa), white clover (Trifolium repens), red clover (Trifolium pratense) or perennial ryegrass (Lolium perenne)

1999 ◽  
Vol 82 (2) ◽  
pp. 139-147 ◽  
Author(s):  
M. Reverter ◽  
T. Lundh ◽  
J. E. Lindberg
Keyword(s):  
Amino Acid ◽  
Medicago Sativa ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Growing Pigs ◽  
Ileal Digestibility

Two experiments were performed with post-valve T-cannulated growing pigs, using five animals in each experiment in a change-over design to evaluate the effect of inclusion of four different dried forage meals on ileal crude protein (CP) and amino acid (AA) digestibilities. The control diets (C1 and C2) were barley-based and the experimental diets were formulated by replacing the barley with 100 or 200 g/kg of either lucerne (Medicago sativa) or white clover (Trifolium repens) meal in Expt 1 and red clover (Trifolium pratense) or perennial ryegrass (Lolium perenne) meal in Expt 2. A decrease (P< 0·05) in the apparent ileal digestibility of CP and most of the essential and nonessential AA was found with the inclusion of lucerne, white clover and perennial ryegrass meal in the barley-based diets. When red clover meal was included, only the apparent ileal digestibilities of CP, leucine, phenylalanine, tyrosine and glutamic acid were found to decrease (P< 0·05). The estimated apparent ileal digestibilities of most essential AA in the forage meals were lower than in the barley-based diets. The ileal flow of glucosamine and ornithine was found to increase (P< 0·05) with increasing proportion of fibre in the diet, suggesting an increase in endogenous N secretions and small-intestinal microbial activity. With the minor changes found for ileal essential AA digestibilities with forage meal inclusion in the diet the present data confirm the potential of forage meals as a source of protein in pig diets.

Selective behaviour in cattle grazing pastures of strips of birdsfoot trefoil and red clover. 1. The effects of relative sward area

2006 ◽  
Vol 144 (2) ◽  
pp. 165-171 ◽  
Author(s):  
C. H. E. C. POLI ◽  
J. HODGSON ◽  
G. P. COSGROVE ◽  
G. C. ARNOLD
Keyword(s):  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Lotus Corniculatus ◽  
Cattle Grazing ◽  
Strong Tendency ◽  
Birdsfoot Trefoil ◽  
Selection Coefficient ◽  
Biting Rate

Alternate 2·4 m wide strips of a mixture of birdsfoot trefoil (Lotus corniculatus L.) with white clover (Trifolium repens L.) (BW), and of red clover (Trifolium pratense L.) (RC) were offered in the proportions (by area) of 0·80[ratio ]0·20, 0·67[ratio ]0·33, 0·33[ratio ]0·67 and 0·20[ratio ]0·80 to yearling heifers in groups of three for periods of 3 days over four replicates in time, balanced for effects of previous treatments. Observations on the distribution of grazing activity and biting rate were made over 3 h periods each evening, and on the morning of Day 2. Biting rates were consistently higher on BW than RC (52·3 v. 46·3±0·59 bites/min, P<0·001). Initially the animals showed a strong tendency to concentrate grazing on the sward of smaller proportional area, but the selection coefficient (log θ) changed from positive to negative with time as herbage on minority strips was depleted. These results are discussed in the context of concepts of partial grazing preference.

Ewe lamb diet selection on plantain (Plantago lanceolata) and on a herb and legume mix, including plantain, chicory (Cichorium intybus), red clover (Trifolium pratense) and white clover (Trifolium repens)

2015 ◽  
Vol 55 (4) ◽  
pp. 515 ◽  
Author(s):  
L. M. Cave ◽  
P. R. Kenyon ◽  
S. T. Morris ◽  
N. Lopez-Villalobos ◽  
P. D. Kemp
Keyword(s):  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Energy Content ◽  
Plantago Lanceolata ◽  
Red Clover ◽  
Diet Selection ◽  
Cichorium Intybus ◽  
Ewe Lambs ◽  
Ewe Lamb

The objective of Experiment 1 was to examine the diet selection and grazing preference of ewe lambs for plantain (Plantago lanceolata), chicory (Cichorium intybus), red clover (Trifolium pratense) and white clover (Trifolium repens). This was examined in a herb and legume sward mix containing plantain, chicory, red clover and white clover between seasons under both hard- and lax-grazing. During early spring, the sward predominately comprised plantain (63%) and chicory (21%) and ewe lambs grazed a greater proportion (P < 0.05) of these species and displayed greater (P < 0.05) grazing preference for plantain and chicory ahead of red and white clover. From late spring onward, ewe lambs had a greater (P < 0.05) defoliation rate and a greater (P < 0.05) grazing preference for red clover than for all other species. This greater selection of red clover was particularly evident in summer and autumn when red clover made a greater (P < 0.05) contribution to the sward composition (8–9%) and the overall sward nutritive value dropped. This supports previous evidence, suggesting that diet selection is intrinsically affected by availability, access and palatability. The objective of Experiment 2 was to determine whether ewe lamb grazing behaviour during late summer was affected by time since a pure plantain sward was previously grazed. Ewe lambs were observed while grazing plantain of varying weeks since previous grazing (3, 6,10 or 16 weeks). Experiment 2 found no difference (P > 0.05) in the average number of ewe lambs grazing each sward of varying weeks since previous grazing. However, ewe lambs grazed the 3-week-old plantain growth to a lower (P < 0.05) post-grazing sward height than the height of the swards of greater number of weeks since previous grazing. Organic matter digestibility and metabolisable energy content did not differ (P > 0.05) among all swards, regardless of weeks since previous grazing. This suggests that the palatability of plantain is not affected by the time since it was last grazed.

scholarly journals Integration of No Tillage and Overseeded Legume Living Mulches for Transplanted Broccoli Production

HortScience ◽  
1996 ◽  
Vol 31 (3) ◽  
pp. 376-380 ◽  
Author(s):  
Michelle L. Infante ◽  
Ronald D. Morse
Keyword(s):  
Weed Control ◽  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Conventional Tillage ◽  
Weed Suppression ◽  
No Tillage ◽  
Hairy Vetch ◽  
Vicia Villosa

Experiments were conducted with `BigSur' broccoli (Brassica oleracea L. var. italica) at two sites in Fall 1993 and at two sites in Spring 1994 on a Hayter loam in southwestern Virginia. Our objectives were to determine the effects of tillage main plots (conventional tillage = CT and no tillage = NT) and weed control subplots [no overseeding or preemergent herbicide, oxyfluorofen, red clover (Trifolium pratense L.), `Dutch' white clover (Trifolium repens L.), and hairy vetch (Vicia villosa Roth)] on broccoli yield and weed suppression. In all sites, weed suppression and marketable broccoli yield with NT were equal to or higher than with CT. Overseeded legume living mulches did not affect broccoli yield in any site compared to the control plots and suppressed weeds as well as the oxyfluorofen in three of the four sites. Thus, the NT systems used in these experiments can suppress weeds and produce high broccoli yields. Also, overseeded legume living mulches can be established effectively after transplanting to suppress weeds without reducing broccoli yield. Chemical name used: 2-choro-1-(3-ethoxy-4-nitrophenoxy)-4-(trifluoromethyl) benzene (oxyfluorofen).

scholarly journals Weed control in establishing mixed swards of clover plantain and chicory

2012 ◽  
Vol 65 ◽  
pp. 59-63 ◽  
Author(s):  
T.L. Gawn ◽  
K.C. Harrington ◽  
C. Matthew
Keyword(s):  
Weed Control ◽  
White Clover ◽  
Trifolium Repens ◽  
Field Trial ◽  
Trifolium Pratense ◽  
Plantago Lanceolata ◽  
Red Clover ◽  
Control Programme ◽  
Conium Maculatum ◽  
Control Options

A field trial was conducted in Palmerston North to assess weed control options for establishing mixed swards of chicory (Cichorium intybus) narrowleaved plantain (Plantago lanceolata) red clover (Trifolium pratense) and white clover (Trifolium repens) a pasture mixture currently popular for finishing lambs Haloxyfop safely controlled grass weeds and mowing twice during the first few months of establishment helped control some broadleaved weeds Flumetsulam was the safest herbicide for controlling broadleaved weeds although it caused severe suppression of plantain initially All other treatments caused unacceptable levels of damage to at least one of the sown species If chicory was not included in the mix then bentazone paraquat/diquat and diuron could be used Options for an integrated weed control programme for the mixture of four species are discussed For young cloverbased pastures hemlock (Conium maculatum) will be controlled better by flumetsulam or bentazone than 24DB

Abstract MP58: Tissue And Sex Specific Effects Of Gstm1 Deletion In Mouse Models

Hypertension ◽  
2021 ◽  
Vol 78 (Suppl_1) ◽  
Author(s):  
Yves Wang ◽  
Nhu Nguyen ◽  
Keith Nehrke ◽  
Paul S Brookes ◽  
Thu H Le
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Mouse Model ◽  
Molecular Mechanisms ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Antioxidant Properties ◽  
Iri Model ◽  
Increased Risk ◽  
Study Gene Expression

The glutathione S-transferase ( Gst ) gene family encodes antioxidant enzymes. In humans, a common null allele deletion variant of GST μ-1 ( GSTM1 ) is highly prevalent across populations and is associated with increased risk and progression of various diseases. Using a Gstm1 knockout (KO) mouse model, we previously showed that KO mice with angiotensin II-induced hypertension (HTN) have increased kidney injury compared to wild-type (WT) controls, mediated by elevated oxidative stress. In the same mouse model, we have recently reported that in a Langendorff-perfused cardiac ischemia-reperfusion injury (IRI) model, where damage is also mediated by oxidative stress, male KO hearts are protected while females are not. Here, we investigated the molecular mechanisms for this difference in male hearts. WT and KO mice of both sexes were studied at 12-20 weeks of age. Hearts were snap frozen at baseline and after 25 min of global ischemia, and kidneys were collected at baseline and 4 weeks following HTN induction. A panel of 18 Gst genes were probed by qPCR from baseline hearts and kidneys of both sexes. Global metabolites were assayed using Metabolon, Inc. from hearts of both sexes and kidneys of males, at both baseline and diseased states. Analysis by qPCR (n = 3/group) showed that male, but not female, KO hearts had upregulation of other Gst s. In contrast, no significant differences between were found in male kidneys. Metabolomics (n = 6/group) detected 695 metabolites in hearts and 926 in kidneys. There were increases in several metabolites in KO vs. WT hearts including those with antioxidant properties. Notably, increases in carnosine and anserine were observed in KO male hearts but not in female hearts, while that of other antioxidant-related metabolites were observed in hearts of both sexes, but not in kidneys. HTN induced significant increases in metabolites in KO vs. WT kidneys in the pathways related to and linking methionine, cysteine, and glutathione, which were not observed in hearts. In this study, gene expression and metabolites suggest that the mechanisms compensating for the loss of GSTM1 are both tissue and sex specific. The resulting differences in antioxidant enzymes and metabolites may explain the unexpected protection for male Gstm1 KO hearts in IRI.

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