scholarly journals A Comparison between FTIR Spectra from HUKE and SH-SY5Y Cell Lines Grown on Different Substrates

2020 ◽  
Vol 10 (24) ◽  
pp. 8825
Author(s):  
Giuseppe Perna ◽  
Vito Capozzi ◽  
Maria Lasalvia
Keyword(s):  
Principal Component ◽  
Medical Diagnostics ◽  
Cellular Level ◽  
Ftir Spectra ◽  
Pathological State ◽  
Absorption Bands ◽  
Ir Radiation ◽  
Glass Slides ◽  
Fingerprint Region ◽  
Cellular Components

In recent years, Fourier Transform Infrared (FTIR) micro-spectroscopy has shown promising potential in medical diagnostics at the cellular level. In fact, FTIR spectra can provide information related to DNA, protein, and lipid content and how such a content changes when a pathological state arises. Most of these information is included in the so-called fingerprint region (1000–1800 cm−1), consisting of several spectral peaks related to vibrational modes occurring inside cellular components. Unfortunately, the slides commonly used in cytology (as the glass microscopy slides and coverslips) are opaque to IR radiation in the fingerprint region, whereas they are transparent for wavenumber values larger than 2000 cm−1, where few and broad spectral absorption bands, mainly due to lipids and proteins, are present. Nonetheless, here we show that FTIR spectra performed in the high wavenumber range 2750–3000 cm−1 can be used to discriminate two different types of cells, one from a normal cell line (Human Keratinocyte, HUKE) and the other from a cancer one (SH-SY5Y). The spectra are discriminated by means of their Principal Component Analysis, according to the PC1 component, and by means of ratiometric analysis, according to the ratio of the intensity of the peak at 2956 cm−1 and that of the peak at 2924 cm−1. The PC1 score values of the HUKE are statistically different from the PC1 score values of SH-SY5Y, whereas the intensity ratio results larger for SH-SY5Y than for HUKE cells. Such results occur for different substrates over which the cells have been grown, including the thick glass slides used for cytological analysis. This result is a further step toward the application of FTIR microspectroscopy in the cytological routine diagnosis.

Quality evaluation of tugaksheeree samples by ATR-FTIR spectroscopy using multicomponent analysis

2020 ◽  
Vol 10 ◽  
Author(s):  
Nikunj D. Patel ◽  
Niranjan S. Kanaki
Keyword(s):  
Principal Component Analysis ◽  
Cluster Analysis ◽  
Quality Evaluation ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Attenuated Total Reflectance ◽  
Chemometric Methods ◽  
Total Reflectance ◽  
Fingerprint Region ◽  
Ftir Technique

Background: Numerous Ayurvedic formulations contains tugaksheeree as key ingredient. Tugaksheereeis the starch gained from the rhizomes of two plants, Curcuma angustifoliaRoxb. (Zingiberaceae) and Marantaarundinacea (MA) Linn. (Marantaceae). Objective: The primary concerns in quality assessment of Tugaksheeree occur due to adulteration or substitution. Method: In current study, Fourier transform infrared (FTIR) technique with attenuated total reflectance (ATR) facility was used to evaluate tugaksheeree samples. Total 10 different samples were studied and transmittance mode was kept to record the spectra devoid of pellets of KBR. Further treatment was given with multi component tools by considering fingerprint region of the spectra. Multivariate analysis was performed by various chemometric methods. Result: Multi component methods like Principal Component Analysis (PCA), and Hierarchical Cluster Analysis (HCA)were used to discriminate the tugaksheeree samples using Minitab software. Conclusion: This method can be used as a tool to differentiate samples of tugaksheeree from its adulterants and substitutes.

scholarly journals Spectroscopy as a useful tool for the identification of changes with time in post-mortem vitreous humor for forensic toxicology purposes

2021 ◽  
Author(s):  
Anna Wójtowicz ◽  
Agata Mitura ◽  
Renata Wietecha-Posłuszny ◽  
Rafał Kurczab ◽  
Marcin Zawadzki
Keyword(s):  
Ir Spectra ◽  
Forensic Toxicology ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Vitreous Humor ◽  
Attenuated Total Reflection ◽  
Glass Slides ◽  
Added Benefit ◽  
Ft Ir ◽  
Non Destructive

AbstractVitreous humor (VH) is an alternative biological matrix with a great advantage of longer availability for analysis due to the lack of many enzymes. The use of VH in forensic toxicology may have an added benefit, however, this application requires rapid, simple, non-destructive, and relatively portable analytical analysis methods. These requirements may be met by Fourier transform infrared spectroscopy technique (FT-IR) equipped with attenuated total reflection accessory (ATR). FT-IR spectra of vitreous humor samples, deposited on glass slides, were collected and subsequent chemometric data analysis by means of Hierarchical Cluster Analysis and Principal Component Analysis was conducted. Differences between animal and human VH samples and human VH samples stored for diverse periods of time were detected. A kinetic study of changes in the VH composition up to 2 weeks showed the distinction of FT-IR spectra collected on the 1st and 14th day of storage. In addition, data obtained for the most recent human vitreous humor samples—collected 3 and 2 years before the study, presented successful discrimination of all time points studied. The method introduced was unable to detect mephedrone addition to VH in the concentration of 10 µg/cm3. Graphic abstract

A Comparison of FT-IR/PA and FT-IR/PBD Spectra of Powders

1986 ◽  
Vol 40 (5) ◽  
pp. 636-641 ◽  
Author(s):  
P. G. Varlashkin ◽  
M. J. D. Low ◽  
G. A. Parodi ◽  
C. Morterra
Keyword(s):  
Probe Beam ◽  
Beam Deflection ◽  
Signal To Noise ◽  
Absorption Bands ◽  
Ir Radiation ◽  
Laser Probe ◽  
Ft Ir ◽  
Better Than

FT-IR photoacoustic (PA) and also photothermal beam deflection (PBD) spectra were recorded with the same particulate samples (graphite, charcoal, aspirin, and silica) under the same conditions in order to compare the quality of the spectra obtainable with the two techniques. A PA cell fitted with windows for the PBD laser probe beam was used, and PA and PBD spectra of each sample were recorded at 8 cm−1 resolution at each of the four different interferometer scan velocities. Although the overall aspects of FT-IR/PA and FT-IR/PBD spectra are the same, the signal-to-noise ratios of PA spectra are appreciably better than those of PBD spectra because PBD detection is more prone to disturbance by vibration than is PA detection. Absorption bands appear at the same wavenumbers in PA and PBD spectra. However, the relative intensities of bands of PBD spectra depend on the absorptive properties of the powdered solids; with weak absorbers, some bands may not be detected at all. PAS can be used with all powders. PBDS is of little or no use for the examination of weakly absorbing powders unless they scatter IR radiation extensively.

Correlated vibrations of the tetrahedral and octahedral complexes and splitting of the absorption bands in FTIR spectra of Li-Zn ferrites

2017 ◽  
Vol 92 ◽  
pp. 267-272 ◽  
Author(s):  
V. Rathod ◽  
A.V. Anupama ◽  
R. Vijaya Kumar ◽  
V.M. Jali ◽  
B. Sahoo
Keyword(s):  
Ftir Spectra ◽  
Absorption Bands

scholarly journals FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Gustavo Jesus Vazquez-Zapien ◽  
Monica Maribel Mata-Miranda ◽  
Virginia Sanchez-Monroy ◽  
Raul Jacobo Delgado-Macuil ◽  
David Guillermo Perez-Ishiwara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Ftir Spectra ◽  
Pancreas Development ◽  
Differentiation Process ◽  
Absorption Bands ◽  
Biophysical Parameter ◽  
Real Time Quantitative Pcr ◽  
Pancreatic Cells ◽  
Embryonic Pancreas

Some of the greatest challenges in stem cells (SCs) biology and regenerative medicine are differentiation control of SCs and ensuring the purity of differentiated cells. In this work, we differentiated mouse pluripotent stem cells (mPSCs) toward pancreatic cells characterizing this differentiation process by molecular and spectroscopic technics. Both mPSCs and Differentiated Pancreatic Cells (DPCs) were subjected to a genetic, phenotypic, and biochemical analysis by real-time quantitative PCR (RT-qPCR), immunocytochemistry, and Fourier Transform Infrared (FTIR) spectroscopy. Cultured mPCSs expressed pluripotent genes and proteins (NanogandSOX2). DPCs expressed endodermal genes (SOX17andPdx1) at day 11, an inductor gene of embryonic pancreas development (Pdx1) at day 17 and pancreas genes and proteins (InsulinandGlucagon) at day 21 of differentiation. Likewise, FTIR spectra of mPSCs and DPCs at different maturation stages (11, 17, and 21 days) were obtained and showed absorption bands related with different types of biomolecules. These FTIR spectra exhibited significant spectral changes agreeing with the differentiation process, particularly in proteins and nucleic acids bands. In conclusion, the obtained DPCs passed through the chronological stages of embryonic pancreas development and FTIR spectra provide a new biophysical parameter based on molecular markers indicating the differentiation process of mPSCs to specialized cells.

scholarly journals Hyperspectral data compression based upon the principal component analysis

2021 ◽  
Vol 45 (2) ◽  
pp. 235-244
Author(s):  
A.S. Minkin ◽  
O.V. Nikolaeva ◽  
A.A. Russkov
Keyword(s):  
Principal Component Analysis ◽  
Data Compression ◽  
Principal Component ◽  
Component Analysis ◽  
Hyperspectral Data ◽  
Gas Absorption ◽  
Absorption Bands ◽  
Spectral Bands ◽  
The Mean ◽  
Retrieval Errors

The paper is aimed at developing an algorithm of hyperspectral data compression that combines small losses with high compression rate. The algorithm relies on a principal component analysis and a method of exhaustion. The principal components are singular vectors of an initial signal matrix, which are found by the method of exhaustion. A retrieved signal matrix is formed in parallel. The process continues until a required retrieval error is attained. The algorithm is described in detail and input and output parameters are specified. Testing is performed using AVIRIS data (Airborne Visible-Infrared Imaging Spectrometer). Three images of differently looking sky (clear sky, partly clouded sky, and overcast skies) are analyzed. For each image, testing is performed for all spectral bands and for a set of bands from which high water-vapour absorption bands are excluded. Retrieval errors versus compression rates are presented. The error formulas include the root mean square deviation, the noise-to-signal ratio, the mean structural similarity index, and the mean relative deviation. It is shown that the retrieval errors decrease by more than an order of magnitude if spectral bands with high gas absorption are disregarded. It is shown that the reason is that weak signals in the absorption bands are measured with great errors, leading to a weak dependence between the spectra in different spatial pixels. A mean cosine distance between the spectra in different spatial pixels is suggested to be used to assess the image compressibility.

scholarly journals Forensic Examination of Inks Extracted from Printed Documents using Fourier Transform Infrared Spectroscopy

2018 ◽  
pp. 10-17
Author(s):  
Paul Ocheje Ameh ◽  
Musa Suud Ozovehe
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Alkyd Resin ◽  
Epoxy Resins ◽  
Ftir Spectra ◽  
Absorption Bands ◽  
Triarylmethane Dyes ◽  
Infra Red ◽  
Aliphatic Ester

Yellow, cyan, magenta and black inks were extracted from documents printed using two common brands of printing cartridge in Nigerian market and analyzed to identify / compare the functional groups present using Fourier Transform Infra-red Spectroscopy (FTIR). The FTIR spectra obtained were found to show highly characteristic absorption bands depending on the composition of the printer inks. Also, the results indicated the presence of triarylmethane dyes, epoxy resins, alkyd resin and esters in all the inks as they are peaks assigned to the vibration of aliphatic ester, asymmetrical and symmetrical stretching. The pure ink and its extract from the same band were also found to exhibit similar FTIR spectra while inks extract from different brands exhibits marked difference in absorption bands. This research can provide valuable information if an admitted sample is provided for comparing with the suspect printed document.

scholarly journals Analysis of functional groups in atmospheric aerosols by infrared spectroscopy: method development for probabilistic modeling of organic carbon and organic matter concentrations

2020 ◽  
Vol 13 (3) ◽  
pp. 1517-1538
Author(s):  
Charlotte Bürki ◽  
Matteo Reggente ◽  
Ann M. Dillner ◽  
Jenny L. Hand ◽  
Stephanie L. Shaw ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Organic Matter ◽  
Organic Carbon ◽  
Functional Groups ◽  
Method Development ◽  
Estimation Method ◽  
Chemical Species ◽  
Monitoring Network ◽  
Ftir Spectra ◽  
Absorption Bands

Abstract. The Fourier transform infrared (FTIR) spectra of fine particulate matter (PM2.5) contain many important absorption bands relevant for characterizing organic matter (OM) and obtaining organic matter to organic carbon (OM∕OC) ratios. However, extracting this information quantitatively – accounting for overlapping absorption bands and relating absorption to molar abundance – and furthermore relating abundances of functional groups to that of carbon atoms poses several challenges. In this work, we define a set of parameters that model these relationships and apply a probabilistic framework to identify values consistent with collocated field measurements of thermal–optical reflectance organic carbon (TOR OC). Parameter values are characterized for various sample types identified by cluster analysis of sample FTIR spectra, which are available for 17 sites in the Interagency Monitoring of Protected Visual Environments (IMPROVE) monitoring network (7 sites in 2011 and 10 additional sites in 2013). The cluster analysis appears to separate samples according to predominant influence by dust, residential wood burning, wildfire, urban sources, and biogenic aerosols. Functional groups calibrations of aliphatic CH, alcohol COH, carboxylic acid COOH, carboxylate COO, and amine NH2 combined together reproduce TOR OC concentrations with reasonable agreement (r=0.96 for 2474 samples) and provide OM∕OC values generally consistent with our current best estimate of ambient OC. The mean OM∕OC ratios corresponding to sample types determined from cluster analysis range between 1.4 and 2.0, though ratios for individual samples exhibit a larger range. Trends in OM∕OC for sites aggregated by region or year are compared with another regression approach for estimating OM∕OC ratios from a mass closure equation of the major chemical species contributing to PM fine mass. Differences in OM∕OC estimates are observed according to estimation method and are explained through the sample types determined from spectral profiles of the PM.

A REVIEW OF PROTOCOLS AND GUIDELINES ADDRESSING THE EXPOSURE OF OCCUPANTS TO ELECTROMAGNETIC FIELD RADIATION (EMFR) IN BUILDINGS

2021 ◽  
Vol 16 (2) ◽  
pp. 55-81
Author(s):  
Shabnam Monadizadeh ◽  
Charles J. Kibert ◽  
Jiaxuan Li ◽  
Junghoon Woo ◽  
Ashish Asutosh ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Human Health ◽  
Health Effects ◽  
Action Plan ◽  
Low Frequency ◽  
Cellular Level ◽  
Future Research ◽  
Ir Radiation ◽  
Health Authorities ◽  
Field Radiation

HIGHLIGHTS ABSTRACT A significant share of the technology that has emerged over the past several decades produces electromagnetic field (EMFR) radiation. Communications devices, household appliances, industrial equipment, and medical equipment and devices all produce EMFR with a variety of frequencies, strengths, and ranges. Some EMFR, such as Extremely Low Frequency (ELF), Radio Frequency (RF), and Ionizing Range (IR) radiation have been shown to have harmful effects on human health. Depending on the frequency and strength of the radiation, EMFR can have health effects at the cellular level as well as at brain, nervous, and cardiovascular levels. Health authorities have enacted regulations locally and globally to set critical values to limit the adverse effects of EMFR. By introducing a more comprehensive field of EMFR study and practice, architects and designers can design for a safer electromagnetic (EM) indoor environment, and, as building and construction specialists, will be able to monitor and reduce EM radiation. This paper identifies the nature of EMFR in the built environment, the various EMFR sources, and its human health effects. It addresses European and US regulations for EMFR in buildings and provides a preliminary action plan. The challenges of developing measurement protocols for the various EMFR frequency ranges and determining the effects of EMFR on building occupants are discussed. This paper argues that a mature method for measuring EMFR in building environments and linking these measurements to human health impacts will foster occupant health and lead to the adequate development of safeguards for occupants of buildings in future research.

scholarly journals Application of FTIR Spectroscopy and HPLC Combined with Multivariate Calibration for Analysis of Xanthones in Mangosteen Extracts

2020 ◽  
Vol 88 (3) ◽  
pp. 35
Author(s):  
Endjang Prebawa Tejamukti ◽  
Widiastuti Setyaningsih ◽  
Irnawati ◽  
Budiman Yasir ◽  
Gemini Alam ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Multivariate Calibration ◽  
Principal Component Regression ◽  
Principal Component ◽  
Ethanolic Extract ◽  
Ftir Spectra ◽  
Partial Least Square ◽  
Least Square ◽  
Coefficient Of Determination ◽  
Garcinia Mangostana

Mangosteen, or Garcinia mangostana L., has merged as an emerging fruit to be investigated due to its active compounds, especially xanthone derivatives such as α -mangostin (AM), γ-mangostin (GM), and gartanin (GT). These compounds had been reported to exert some pharmacological activities, such as antioxidant and anti-inflammatory, therefore, the development of an analytical method capable of quantifying these compounds should be investigated. The aim of this study was to determine the correlation between FTIR spectra and HPLC chromatogram, combined with chemometrics for quantitative analysis of ethanolic extract of mangosteen. The ethanolic extract of mangosteen pericarp was prepared using the maceration technique, and the obtained extract was subjected to measurement using instruments of FTIR spectrophotometer at wavenumbers of 4000–650 cm−1 and HPLC, using a PDA detector at 281 nm. The data acquired were subjected to chemometrics analysis of partial least square (PLS) and principal component regression (PCR). The result showed that the wavenumber regions of 3700–2700 cm−1 offered a reliable method for quantitative analysis of GM with coefficient of determination (R2) 0.9573 in calibration and 0.8134 in validation models, along with RMSEC value of 0.0487% and RMSEP value 0.120%. FTIR spectra using the second derivatives at wavenumber 3700–663 cm−1 with coefficient of determination (R2) >0.99 in calibration and validation models, along with the lowest RMSEC value and RMSEP value, were used for quantitative analysis of GT and AM, respectively. It can be concluded that FTIR spectra combined with multivariate are accurate and precise for the analysis of xanthones.

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