scholarly journals Protein Engineering of Mung Bean (Vigna radiata (L.) Wilczek) 8Sα Globulin with Lactostatin

2020 ◽  
Vol 10 (24) ◽  
pp. 8787
Author(s):  
Ma. Carla Gamis ◽  
Lawrence Yves Uy ◽  
Antonio Laurena ◽  
Wilma Hurtada ◽  
Mary Ann Torio
Keyword(s):  
Liquid Chromatography ◽  
Bile Acid ◽  
Protein Engineering ◽  
Mung Bean ◽  
Binding Capacity ◽  
Protein Structures ◽  
Bioactive Peptide ◽  
Wild Type ◽  
Cholesterol Lowering ◽  
Bile Acid Binding

Mung bean is a well-known good source of protein. To increase its bioactivity, economic value, and nutritional content as a functional food and food additive, lactostatin (IIAEK), a cholesterol-lowering bioactive peptide, was engineered into mung bean 8Sα globulin, a major storage protein. The results showed that the mutated 8Sα globulin has a significant bile acid binding capacity (cholesterol-lowering activity) up to 47.25%. Moreover, superimposed mutant (Mut2) and wild-type (Wt) 3D protein structures showed a 93–97% identity, indicating that the mutant proteins are stable. Ultra-performance liquid chromatography(UPLC)-based assay showed similar retention time for wild-type and mutant protein samples. Both IIAEK peptide standard and Mut2 digest had comparable baseline peaks corresponding to the same molecular size based on the liquid chromatography-mass spectrometry (LC-MS) data. A 573.36-Da mass spectrum was seen in Mut2, which indicates that Mut2 8Sα globulin has been successfully mutated and digested to release the bioactive peptide, IIAEK. In vitro bile acid binding capacity showed that the 6-h Wt and 12-h engineered protein (Mut2) digests had the highest activity. Lastly, potential allergenicity was checked in the Allergen Database for Food Safety (ADFS) and the AllerBase database, and the IIAEK peptide matched the Bos d 5 epitopes. This study provides a strong foundation and basis for mung bean nutrition improvement, development of cholesterol-lowering food supplements, and protein engineering of other food proteins.

scholarly journals Plasma cholesterol lowering action of bile acid binding polymers in experimental animals

1960 ◽  
Vol 1 (5) ◽  
pp. 469-473 ◽  
Author(s):  
DavidM. Tennent ◽  
Henry Siegel ◽  
MaryE. Zanetti ◽  
GuntherW. Kuron ◽  
WaltherH. Ott ◽  
...  
Keyword(s):  
Bile Acid ◽  
Plasma Cholesterol ◽  
Cholesterol Lowering ◽  
Experimental Animals ◽  
Bile Acid Binding

scholarly journals Mechanisms of cholesterol-lowering effects of dietary insoluble fibres: relationships with intestinal and hepatic cholesterol parameters

2005 ◽  
Vol 94 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Ariëtte M. van Bennekum ◽  
David V. Nguyen ◽  
Georg Schulthess ◽  
Helmut Hauser ◽  
Michael C. Phillips
Keyword(s):  
Bile Acid ◽  
Food Intake ◽  
Serum Cholesterol ◽  
Cholesterol Absorption ◽  
Cholesterol Homeostasis ◽  
Hepatic Cholesterol ◽  
Intestinal Cholesterol Absorption ◽  
Cholesterol Lowering ◽  
Dietary Fibres ◽  
Bile Acid Binding

Fibres with a range of abilities to perturb cholesterol homeostasis were used to investigate how the serum cholesterol-lowering effects of insoluble dietary fibres are related to parameters of intestinal cholesterol absorption and hepatic cholesterol homeostasis in mice. Cholestyramine, chitosan and cellulose were used as examples of fibres with high, intermediate and low bile acid-binding capacities, respectively. The serum cholesterol levels in a control group of mice fed a high fat/high cholesterol (HFHC) diet for 3 weeks increased about 2-fold to 4·3 mm and inclusion of any of these fibres at 7·5 % of the diet prevented this increase from occurring. In addition, the amount of cholesterol accumulated in hepatic stores due to the HFHC diet was reduced by treatment with these fibres. The three kinds of fibres showed similar hypocholesterolaemic activity; however, cholesterol depletion of liver tissue was greatest with cholestyramine. The mechanisms underlying the cholesterol-lowering effect of cholestyramine were (1) decreased cholesterol (food) intake, (2) decreased cholesterol absorption efficiency, and (3) increased faecal bile acid and cholesterol excretion. The latter effects can be attributed to the high bile acid-binding capacity of cholestyramine. In contrast, incorporation of chitosan or cellulose in the diet reduced cholesterol (food) intake, but did not affect either intestinal cholesterol absorption or faecal sterol output. The present study provides strong evidence that above all satiation and satiety effects underlie the cholesterol-lowering properties of insoluble dietary fibres with moderate or low bile acid-binding capabilities.

Bile acid binding capacity of fish protein hydrolysates from discard species of the West Mediterranean Sea

2015 ◽  
Vol 6 (4) ◽  
pp. 1261-1267 ◽  
Author(s):  
Raúl Pérez-Gálvez ◽  
Pedro J. García-Moreno ◽  
Rocío Morales-Medina ◽  
Antonio Guadix ◽  
Emilia M. Guadix
Keyword(s):  
Bile Acid ◽  
Mediterranean Sea ◽  
Binding Capacity ◽  
Protein Hydrolysates ◽  
Fish Protein ◽  
The West ◽  
Bile Acid Binding ◽  
Fish Protein Hydrolysates

Fish protein hydrolysates from six fish discard species in the West Mediterranean Sea were tested for theirin vitrobile acid binding capacity.

A comparison study on polysaccharides extracted from Laminaria japonica using different methods: structural characterization and bile acid-binding capacity

2017 ◽  
Vol 8 (9) ◽  
pp. 3043-3052 ◽  
Author(s):  
Jie Gao ◽  
Lianzhu Lin ◽  
Baoguo Sun ◽  
Mouming Zhao
Keyword(s):  
Bile Acid ◽  
Rheological Properties ◽  
Structural Characterization ◽  
Binding Capacity ◽  
Laminaria Japonica ◽  
Extraction Methods ◽  
Comparison Study ◽  
Bile Acid Binding

The structural characterization, rheological properties and bile acid-binding capacity of LP obtained by seven different extraction methods were investigated.

Safety and Efficacy of Long-Term Diet and Diet Plus Bile Acid-Binding Resin Cholesterol-Lowering Therapy in 73 Children Heterozygous for Familial Hypercholesterolemia

PEDIATRICS ◽  
1986 ◽  
Vol 78 (2) ◽  
pp. 338-348 ◽  
Author(s):  
Charles J. Glueck ◽  
Margot J. Mellies ◽  
Mark Dine ◽  
Tammy Perry ◽  
Peter Laskarzewski
Keyword(s):  
Bile Acid ◽  
Familial Hypercholesterolemia ◽  
Plasma Cholesterol ◽  
Total Plasma Cholesterol ◽  
Total Plasma ◽  
Normal Children ◽  
Cholesterol Lowering ◽  
Bile Acid Binding

Our specific aim was to examine the efficacy and safety of long-term cholesterol-lowering diet and bile acid-binding resin therapy in 73 children heterozygous for familial hypercholesterolemia (FH). We prospectively followed accretion of height and weight in 40 FH children for 5.8 years on diet alone and in 33 FH children for 4.3 years on diet and bile acid-binding resins (8 to 20 g/d). In 67 of these 73 children, sequential data on plasma choleterol lowering was obtained, including 32 children on diet plus bile acid-binding resins and 35 on diet alone. For all 73 children, median age, sex, and race-specific percentiles for height and weight at entry were 50 and 50, respectively, and, 5.7 years later, were unchanged at 50 and 50. Initial and final percentiles for height (r = .76, P < .001) and weight (r = .70, P < .001) were closely correlated. Percentile distributions for height and weight at entry into the study did not differ from those at the end of follow-up (P > .1), in both the 40 FH children on diet alone and the 33 on diet plus bile acid-binding resins. Tracking of height and weight did not differ in the 40 children on diet alone v the 33 on diet plus bile acidbinding resins (P > .1). During 6 years of follow-up there were no significant differences in the percentage of serial, postbaseline measurements for height which were either less than or greater than or equal to baseline percentiles, comparing 40 FH children on diet alone, 33 FH children on diet plus resin, and 39 normal children (on ad libitum diet). FH children on diet or plus resin had a smaller percentage of weight measurements equal to or more than baseline percentiles than normals on follow-up (P < .01), probably reflecting restriction of total fat intake to < 35% of calories. On diet alone, 32 FH children had total plasma cholesterol of 307 ± 8 mg/dL (mean ± SE); bile acidbinding resins were added to diet in these children at an average age of 11.5 years, with this regimen maintained for 4.6 ± 0.4 years, leading to a mean reduction in total plasma cholesterol of 12.5% ± 2% beyond the effects of diet alone (P<.01). On diet plus bile acid-binding resins, 44% of children had a reduction of total plasma cholesterol ≤14%; 28% had a reduction ≥21% (beyond the effects of diet alone.) Thirty-five FH children (starting at 11.7 years of age) were treated with diet alone. Their mean total plasma cholesterol at baseline was 243 ± 6 mg/dl and decreased 9.6% ± 2% (P<.01) after an average of 4.5 ± 0.5 years of diet. On diet alone, 29% of children had a reduction of total plasma cholesterol ≥14%, and 23% had a reduction ≥21%. Long-term diet and bile acid-binding resin therapy did not affect normal growth in 73 FH children and was effective in reducing total plasma cholesterol levels within ranges previously shown to have efficacy in reducing coronary heart disease events in hypercholesterolemic men.

scholarly journals Experimental Model of Hypercholesterolemia in Common Marmosets and Cholesterol-Lowering Effects of Bile Acid-Binding Resins

2000 ◽  
Vol 82 ◽  
pp. 268
Author(s):  
Satoshi Unakami ◽  
Yoshimasa Kurata ◽  
Takeshi Kawasuso ◽  
Megumi Tamagawa ◽  
Hiromi Iizuka
Keyword(s):  
Bile Acid ◽  
Experimental Model ◽  
Cholesterol Lowering ◽  
Common Marmosets ◽  
Bile Acid Binding
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