scholarly journals Comparative Study of the Bioactive Properties and Elemental Composition of Red Clover (Trifolium pratense) and Alfalfa (Medicago sativa) Sprouts during Germination

2020 ◽  
Vol 10 (20) ◽  
pp. 7249
Author(s):  
Elena Roxana Chiriac ◽  
Carmen Lidia Chiţescu ◽  
Claudia Sandru ◽  
Elisabeta-Irina Geană ◽  
Mariana Lupoae ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Plant Species ◽  
Trifolium Pratense ◽  
Statistical Processing ◽  
Red Clover ◽  
Elemental Content ◽  
Individual Species ◽  
Total Polyphenols ◽  
Alfalfa Sprouts ◽  
The Individual

Considering the growing interest in functional foods, the identification of the individual species of elements is of great importance in understanding specific nutraceutical properties. The present study aims to compare the dynamic of the elemental content (K, Na, Ca, Mg, Fe, Cu, Zn, Mn, Ni, and Se), total polyphenols, and antioxidant activity of Trifolium pratense L. and Medicago sativa L. sprouts in different germination stages. The elemental profile was established by atomic absorption spectroscopy after the microwave acid digestion of the samples, while total polyphenols and anti-radical activity were evaluated by UV-Vis spectroscopic methods. Phenolic compounds and anti-radical activity of both alfalfa and red clover sprouts varied with germination stages. Germination can significantly increase the anti-radical activity in the first 3 days of germination, followed by a decline in the following days. An increase in total polyphenols was noticed, starting from the second day of germination in both plant species. There were significant (p < 0.05) differences for Ca, Na, Fe, K, Zn, and Mg contents among the sprouts, depending on the germination stage and plant species. The calcium contents of alfalfa ranged between 200.74 µg/g DW (raw seeds) and 2765.31 µg/g DW (sprouted), while in red clover between 250.83 µg/g DW and 601.59 µg/g DW. Maximum selenium content in alfalfa sprouts, reached in the 3rd day of germination (11.42 µg/g DW), exceeded the maximum value measured in red clover (9.42 µg/g DW). The data were subject to statistical processing using analysis of variance (ANOVA), multivariate analysis (PCA) and hierarchical clustering analysis (HCA).

scholarly journals Precrop Effect of Red Clover (Trifolium pratense L.) and Alfalfa (Medicago sativa L.) on the Population Development of the Northern Root-Knot Nematode Meloidogyne hapla Chitwood, 1949 and on Succeeding Crops—A Pot Study

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 722
Author(s):  
Renata Dobosz ◽  
Roman Krawczyk
Keyword(s):  
Population Density ◽  
Medicago Sativa ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Continuous Cultivation ◽  
Nematode Population ◽  
Meloidogyne Hapla ◽  
Root Knot Nematode ◽  
Crop Species ◽  
Tomato Growth

The northern root-knot nematode, Meloidogyne hapla, is a major pest of many crop species. The objective of the study was to determine how M. hapla population dynamics is affected by two precrops, i.e., Trifolium pratense and Medicago sativa, in three crop durations: one, two and three years of continuous cultivation. Moreover, we set ourselves the task of evaluating the effect of the legume precrop soil on the growth of the succeeding tomato plant (Solanum lycopersicum) and on the nematode population. The experiment was performed outdoors in pots with naturally infected soil. Both precrop species investigated were found to modify the J2 nematode population density in the soil. The galls and nematode females with egg masses were observed on the roots of both studied plant species at the end of each growing season. They appeared to be more abundant on the red clover roots than on those of the alfalfa. The obtained data indicate that the spring soil sampling is more appropriate for the estimation of the M. hapla population density in the red clover precrop soil. The legume precrop soil had a limiting effect on tomato growth and fruit yield. The nematode population negatively influenced tomato growth. The experiment revealed that tomato plants could be planted in alfalfa precrop soil following at least three years of continuous alfalfa cultivation. The same cannot be said of the cultivation of red clover as a precrop for tomatoes.

Effect of flower complexity on relearning flower-handling skills in bumble bees

1995 ◽  
Vol 73 (11) ◽  
pp. 2052-2058 ◽  
Author(s):  
Robert J. Gegear ◽  
Terence M. Laverty
Keyword(s):  
Plant Species ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Lythrum Salicaria ◽  
Bumble Bees ◽  
Access Time ◽  
Interference Effects ◽  
Flower Constancy ◽  
Flower Handling ◽  
Interference Hypothesis

Pollinators often forage sequentially among the flowers of the same plant species while bypassing flowers of other rewarding species. Darwin proposed that it is more efficient for pollinators to remain constant to one plant species because switching to a second species interferes with their ability to recall a previously learned flower-handling technique. This interference hypothesis was tested using Bombus impatiens workers. Bees that had learned to handle one type of flower (species A) were retested on species A after they had learned to handle a second type of flower (species B). Interference effects were detected by comparing flower access times (time to insert the tongue into the flower) during the retesting period with initial access times on species A. Bees retested on both simple (red clover, Trifolium pratense) and complex (toadflax, Linaria vulgaris) flowers showed no evidence of interference after learning simple-flowered plant species (blueweed, Echium vulgare; purple loosestrife, Lythrum salicaria). However, bees relearning the complex flowers of toadflax showed a 2.2-s (81%) increase over their initial access time after switching to a second complex-flowered species (orange touch-me-not, Impatiens capensis). These results suggest that the interference effects incurred by bees switching between toadflax and orange touch-me-not under biologically realistic conditions are relatively small, and are unlikely to account for flower constancy in bumble bees.

COLOR INFRARED AERIAL PHOTOGRAPHY TO IDENTIFY FORAGE LEGUMES IN HAY FIELDS

1981 ◽  
Vol 61 (2) ◽  
pp. 331-336 ◽  
Author(s):  
P. K. BASU
Keyword(s):  
Trifolium Pratense ◽  
Red Clover ◽  
Aerial Photographs ◽  
Forage Legume ◽  
Image Characteristics ◽  
Mixed Crop ◽  
Trifolium Pratense L ◽  
Medicago Sativa L ◽  
The Individual ◽  
Agricultural Areas

In September 1977 and 1978, color infrared (CIR) aerial photographs of mixed agricultural areas (approx. 132.7 km2) near Vernon and Carp, Ontario, and of forage legume test plots at Ottawa were taken at a scale of 1:6000. Extensive ground surveys were conducted within 2 wk after the photographic flights to verify CIR images of alfalfa (Medicago sativa L.), red clover (Trifolium pratense L.), trefoil (Lotus corniculatus L.), vetch (Vicia spp.) grasses and their mixtures in the original transparencies. A number of photographic image characteristics of legumes and grasses have been illustrated by which a reliable identification and subsequent quantification of legume components were made, particularly in fields that showed decline of a crop or when the individual components grew as separate patches in the same field. Photo-interpretation difficulties existed in distinguishing the components in a very uniformly mixed crop and recently cut fields.

COMPARISON OF YIELD COMPONENTS OF RED CLOVER AND ALFALFA

1988 ◽  
Vol 68 (1) ◽  
pp. 103-114 ◽  
Author(s):  
S. R. BOWLEY ◽  
C. T. DOUGHERTY ◽  
N. L. TAYLOR ◽  
P. L. CORNELIUS
Keyword(s):  
Medicago Sativa ◽  
Yield Components ◽  
Trifolium Pratense ◽  
Total Yield ◽  
Red Clover ◽  
Herbage Yield ◽  
Trifolium Pratense L ◽  
Medicago Sativa L ◽  
Stem Development ◽  
Selection For

Yield components of five regrowth cycles, two in 1981 and three in 1982, of three red clover (Trifolium pratense L.) populations and three alfalfa (Medicago sativa L.) cultivars were compared in swards. Red clover left more stubble but produced less herbage and accumulated total yield (herbage, stubble and 10 cm depth root) at a slower rate than alfalfa during most regrowths. The percent leaf at flowering of red clover was similar to that of alfalfa. Maximum LAI declined during successive regrowths each year. Maximum crop growth rates were 30 and 34 g m−2 d−1 for red clover and alfalfa, respectively. Stem development (canopy height) followed patterns of accumulation of herbage yield. The initiation of stems was slower in red clover and stem populations were less than that of alfalfa. Selection for faster stem initiation rates, higher stem populations, and reduced partitioning of dry matter towards stem bases which would be left as stubble were suggested as ways of increasing the herbage yield of red clover.Key words: Trifolium pratense L., Medicago sativa L., yield components, growth analysis clover (red), alfalfa

scholarly journals Ileal amino acid digestibilities in pigs of barley-based diets with inclusion of lucerne (Medicago sativa), white clover (Trifolium repens), red clover (Trifolium pratense) or perennial ryegrass (Lolium perenne)

1999 ◽  
Vol 82 (2) ◽  
pp. 139-147 ◽  
Author(s):  
M. Reverter ◽  
T. Lundh ◽  
J. E. Lindberg
Keyword(s):  
Amino Acid ◽  
Medicago Sativa ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Growing Pigs ◽  
Ileal Digestibility

Two experiments were performed with post-valve T-cannulated growing pigs, using five animals in each experiment in a change-over design to evaluate the effect of inclusion of four different dried forage meals on ileal crude protein (CP) and amino acid (AA) digestibilities. The control diets (C1 and C2) were barley-based and the experimental diets were formulated by replacing the barley with 100 or 200 g/kg of either lucerne (Medicago sativa) or white clover (Trifolium repens) meal in Expt 1 and red clover (Trifolium pratense) or perennial ryegrass (Lolium perenne) meal in Expt 2. A decrease (P< 0·05) in the apparent ileal digestibility of CP and most of the essential and nonessential AA was found with the inclusion of lucerne, white clover and perennial ryegrass meal in the barley-based diets. When red clover meal was included, only the apparent ileal digestibilities of CP, leucine, phenylalanine, tyrosine and glutamic acid were found to decrease (P< 0·05). The estimated apparent ileal digestibilities of most essential AA in the forage meals were lower than in the barley-based diets. The ileal flow of glucosamine and ornithine was found to increase (P< 0·05) with increasing proportion of fibre in the diet, suggesting an increase in endogenous N secretions and small-intestinal microbial activity. With the minor changes found for ileal essential AA digestibilities with forage meal inclusion in the diet the present data confirm the potential of forage meals as a source of protein in pig diets.

Bromoxynil, Chloroxynil, and 2,4-DB for Establishing Alfalfa and Medium Red Clover

Weed Science ◽  
1972 ◽  
Vol 20 (2) ◽  
pp. 140-142 ◽  
Author(s):  
Elroy J. Peters ◽  
S. A. Lowance
Keyword(s):  
Medicago Sativa ◽  
Butyric Acid ◽  
Trifolium Pratense ◽  
No Reduction ◽  
Chenopodium Album ◽  
Red Clover ◽  
Ambrosia Artemisiifolia ◽  
Time Of Application ◽  
Excellent Control ◽  
Significant Yield

When almost no broadleaf weeds were present, 0.56 kg/ha of the butoxyethanol ester and 0.84 kg/ha of the dimethylamine salt of 4-(2,4-dichlorophenoxy)butyric acid (2, 4-DB) caused no yield reductions of seedling alfalfa (Medicago sativa L.); but 0.56 kg/ha of 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil) caused slight reductions. Rates of bromoxynil above 0.56 kg/ha caused significant yield reductions of alfalfa. Broadleaf weeds, when present, shielded alfalfa from the bromoxynil so that reduction in yields of broadleaf weeds and increases in alfalfa were similar to those obtained with 2,4-DB. At equivalent rates 3,5-dichloro-4-hydroxybenzonitrile (hereinafter referred to as chloroxynil) was less effective than bromoxynil for controlling broadleaf weeds and also was less injurious to alfalfa. When no weeds were present at time of application, bromoxynil at a rate of 0.56 kg/ha or greater and chloroxynil at 0.84 and 1.12 kg/ha significantly reduced yields of red clover (Trifolium pratense L.). When weeds were present bromoxynil did not reduce yields of red clover until the 1.12-kg rate was reached. Chloroxynil did not reduce yields at rates as high as 1.12 kg/ha. Excellent control of common lambsquarter (Chenopodium album L.) and common ragweed (Ambrosia artemisiifolia L.) was obtained, and no reduction in alfalfa or red clover occurred with 0.28 kg/ha of bromoxynil or chloroxynil mixed with 0.56 kg/ha of 2,4-DB ester.

scholarly journals The bees of the genus Hylaeus Fabricius, 1793 of Turkey, with keys to the subgenera and species (Hymenoptera: Anthophila, Colletidae)

2020 ◽  
Vol 70 (2) ◽  
pp. 273-346
Author(s):  
Hikmet Özbek ◽  
Holger H. Dathe
Keyword(s):  
Plant Species ◽  
Rare Species ◽  
Individual Species ◽  
New Combinations ◽  
Numerous Species ◽  
Distribution Maps ◽  
Centre Of Diversity ◽  
Geographical Regions ◽  
The World ◽  
The Individual

The paper presents data of around 4000 previously unpublished specimens, collected in various parts of the country during the last decades. With literature sources, a total of 86 species of the genus Hylaeus Fabricius, 1793 from 10 subgenera are compiled for Turkey. New for Turkey are 11 species: Hylaeus (Dentigera) kahri Förster, 1871, H. (Dentigera) pallidicornis Morawitz, 1876, H. (Hylaeus) deceptorius (Benoist, 1959), H. (Hylaeus) gracilicornis (Morawitz, 1867), H. (Hylaeus) paulus Bridwell, 1919, H. (Hylaeus) trisignatus Morawitz, 1876, H. (Nesoprosopis) pectoralis Förster, 1871, H. (Prosopis) incongruus Förster, 1871, H. (Prosopis) trinotatus (Pérez, 1896), H. (Prosopis) variolaris Morawitz, 1876 and H. (Spatulariella) sulphuripes (Gribodo, 1894). No new specimens could be found of 13 species which had been detected formerly. Our knowledge on the distribution of numerous species is greatly expanded. The characteristics of distribution are defined for the individual species. For example, H. meridionalis is the most widespread with records from 46 provinces covering all geographical regions of the country, while many other species are only known from one province, sometimes from a single record. The eastern part of Turkey, in particular the province of Hakkâri, proved to be an important centre of diversity for Hylaeus species. For a number of species the valid names had to be revised. Distribution maps are presented for the newly recorded and rare species. Frequently visited plant species are mentioned. Keys to the subgenera and species of Turkish Hylaeus are provided. Nomenclatural acts All species described by Warncke in 1981 and 1992 from Turkey in the genus Prosopis F. have not yet been revised and formally combined with the valid genus name Hylaeus F. There are however a number of online checklists, which have already registered most of these names under Hylaeus. Representatives of these checklists were checked here: The World Bee Checklist, integrated into the ITIS database (ITIS 2020) and Discover Life (Ascher & Pickering 2019). As new combinations, only the missing names are added here.Hylaeus (Dentigera) giresunus (Warncke, 1992) – comb. et stat. nov.Hylaeus (Hylaeus) trochilus (Warncke, 1992) – comb. et stat. nov.Hylaeus (Paraprosopis) decaocto (Warncke, 1992) – comb. nov.Hylaeus (Paraprosopis) socheri Dathe, 2010 = H. (Paraprosopis) decaocto (Warncke, 1992) – syn. nov.Prosopis styriaca crecca Warncke, 1992 = Hylaeus (Paraprosopis) styriacus Förster, 1871 – stat. nov.Hylaeus (Spatulariella) planulus (Warncke, 1981) – comb. nov.Hylaeus (Spatulariella) tauricus Warncke, 1981 – comb. nov.

Food-Gathering Behaviour of Honey, Bumble, and Leaf-Cutter Bees (Hymenoptera: Apoidea) in Alberta

1961 ◽  
Vol 93 (6) ◽  
pp. 409-419 ◽  
Author(s):  
G. A. Hobbs ◽  
W. O. Nummi ◽  
J. F. Virostek
Keyword(s):  
Medicago Sativa ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Bumble Bees ◽  
Western Canada ◽  
Effective Pollinators

In the prairie region of Western Canada certain species of bumble bees are the principal pollinators of red clover, Trifolium pratense L. (Hobbs, 1957). Certain bumble and leaf-cutter bees are the only effective pollinators of alfalfa, Medicago sativa L., in Western Canada (Hobbs and Lilly, 1954; Hobbs, 1956; Peck and Bolton, 1946; Stephen, 1955). Unfortunately, bumble and leaf-cutter bees are seldom very abundant.

scholarly journals Comparison of the Polyphenolic Profile of Medicago sativa L. and Trifolium pratense L. Sprouts in Different Germination Stages Using the UHPLC-Q Exactive Hybrid Quadrupole Orbitrap High-Resolution Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2321 ◽  
Author(s):  
Elena Roxana Chiriac ◽  
Carmen Lidia Chiţescu ◽  
Daniela Borda ◽  
Mariana Lupoae ◽  
Cerasela Elena Gird ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Trifolium Pratense ◽  
High Resolution Mass Spectrometry ◽  
Estrogenic Activity ◽  
Red Clover ◽  
Rapid Separation ◽  
Fragment Ions ◽  
Trifolium Pratense L ◽  
Medicago Sativa L ◽  
Identification And Quantification

Identification and quantification of polyphenols in plant material are of great interest since they make a significant contribution to its total bioactivity. In the present study, an UPLC-Orbitrap-MS/MS approach using the variable data acquisition mode (vDIA) was developed and applied for rapid separation, identification, and quantification of the main polyphenolic compounds in Medicago sativa L. and Trifolium pratense L. sprouts in different germination stages. Based on accurate MS data and fragment ions identification strategy, a total of 29 compounds were identified by comparing their accurate masses, fragment ions, retention times, and literatures. Additionally, a number of 30 compounds were quantified by comparing to the reference standards. Data were statistically analysed. For both plant species, the sprouts of the third germination day are valuable sources of bioactive compounds and could be used in phytotherapy and nutrition. Although Trifolium pratense L. (Red Clover) is considered to be a reference for natural remedies in relieving menopause disorders, alfalfa also showed a high level of biological active compounds with estrogenic activity.

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