scholarly journals Optimization of the Antioxidant Activities of Mixtures of Melastomataceae Leaves Species (M. malabathricum Linn Smith, M. decemfidum, and M. hirta) Using a Simplex Centroid Design and Their Anti-Collagenase and Elastase Properties

2020 ◽  
Vol 10 (19) ◽  
pp. 7002
Author(s):  
Nur Fauwizah Azahar ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
Paiman Bawon ◽  
Mohd Izuan Effendi Halmi
Keyword(s):  
High Performance ◽  
Hplc Analysis ◽  
Antioxidant Activities ◽  
Control Point ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Set Up ◽  
Rutin And Quercetin

In this study, a simplex-centroid mixture design (SCMD) approach was used to select the optimal proportions of three different Melastomataceae leaves species (M. malabathricum, M. decemfidum, and M. hirta) extracts to determine the optimum antioxidant activities of total phenolic compound (TPC) and ABTS (2, 2′-azino-bis (ethylbenzthiazoline-6-sulfonic acid)) radical-scavenging activities. Twelve experimental designs were set up, consisting of points that were equally weighted mixtures of 0 to 1 components, which were pure blends (1, 0, …, 0), binary blends (1/2, 1/2, 0, …, 0), tertiary blends (1/3, 1/3, 1/3, 0, …, 0), and a control point. The in vitro anti-elastase and collagenase activities were evaluated in order to determine the anti-aging efficacy of the optimized mixture extracts. A high-performance liquid chromatography (HPLC) analysis was employed to identify the flavonoid content (rutin and quercetin) present in the optimized mixture extracts. The results showed that the best proportions of the optimum phenolic compounds and ABTS activity corresponded to 30%, 40%, and 30% of M. malabathricum, M. hirta, and M. decemfidum leaf extracts, respectively. The in vitro anti-collagenase and elastase activity evaluation of the optimized mixture extracts showed 70% inhibition against both elastase and collagenase enzymes. The HPLC analysis revealed two flavonoids (rutin and quercetin) at retention time 7.770 and 8.769 min, respectively, in the mixture extracts. This study suggests the potential exploitation of mixtures of Melastomataceae leaves (M. malabathricum, M. decemfidum, and M. hirta) as cosmetic ingredients for antioxidant and anti-wrinkles applications.

scholarly journals Antioxidant activities and A549 lung adenocarcinomic cells against capacity from various extracts of Agrimonia pilosa Ledeb

2018 ◽  
Vol 4 (3) ◽  
pp. 114-119
Author(s):  
Quang Ung Le ◽  
◽  
Horng Liang Lay ◽  
Ming Chang Wu ◽  
◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
A549 Cells ◽  
Total Phenolic ◽  
Root Extract ◽  
Inhibitory Capacity ◽  
Ethanol Extracts

Agrimonia pilosa Ledeb (AL) has received considerable attention as a herbal medicine for its applications in ethnopharmacology with heath benefits. This study aimed to investigate antioxidant activities and A549 growth inhibitory capacity from its root extract (RE) and aerial parts extract (AE). The 50% ethanol extracts were used for the tests. The total polyphenol content and the antioxidant effects comprising ABTS+ and DPPH free radical scavenging activities were evaluated. Phenolic compounds in the extracts were isolated using high performance liquid chromatography (HPLC). Lactate dehydrogenase released in medium was also evaluated. Total phenolic and flavonoid content, and in vitro antioxidant potential of the RE were higher (p<0.01) than that of the AE. Two individual phenolic compounds consisting of 4-hydroxybenzoic acid and p-coumaric acid were firstly identified in both by HPLC. The RE exhibited higher A549 inhibitory capacity compared to the AE and activated the apoptotic proteins of bcl-2, bax, bad, caspase-3 and caspase-9 in A549. In conclusion, the AL extracts were more effective in antioxidant and A549 cells inhibitory capacity.

scholarly journals Investigation of in vitro antioxidant activities of Sri Lankan grown Glinus oppositifolius (L.) Aug. DC

2018 ◽  
Vol 4 (3) ◽  
pp. 120-122
Author(s):  
M.N.F. Rizniya ◽  
◽  
A.H.M. Mawjood ◽  
L.D.A.M. Arawwawala ◽  
◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
A549 Cells ◽  
Total Phenolic ◽  
Root Extract ◽  
Inhibitory Capacity ◽  
Ethanol Extracts

Agrimonia pilosa Ledeb (AL) has received considerable attention as a herbal medicine for its applications in ethnopharmacology with heath benefits. This study aimed to investigate antioxidant activities and A549 growth inhibitory capacity from its root extract (RE) and aerial parts extract (AE). The 50% ethanol extracts were used for the tests. The total polyphenol content and the antioxidant effects comprising ABTS+ and DPPH free radical scavenging activities were evaluated. Phenolic compounds in the extracts were isolated using high performance liquid chromatography (HPLC). Lactate dehydrogenase released in medium was also evaluated. Total phenolic and flavonoid content, and in vitro antioxidant potential of the RE were higher (p<0.01) than that of the AE. Two individual phenolic compounds consisting of 4-hydroxybenzoic acid and p-coumaric acid were firstly identified in both by HPLC. The RE exhibited higher A549 inhibitory capacity compared to the AE and activated the apoptotic proteins of bcl-2, bax, bad, caspase-3 and caspase-9 in A549. In conclusion, the AL extracts were more effective in antioxidant and A549 cells inhibitory capacity.

scholarly journals Polyphenols and Alkaloids in Byproducts of Longan Fruits (Dimocarpus Longan Lour.) and Their Bioactivities

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1186 ◽  
Author(s):  
Ya-Yuan Tang ◽  
Xue-Mei He ◽  
Jian Sun ◽  
Chang-Bao Li ◽  
Li Li ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Research Result ◽  
Radical Scavenging ◽  
Gastric Fluid ◽  
Simulated Gastric Fluid ◽  
Total Phenolic ◽  
Functional Components

The longan industry produces a large amount of byproducts such as pericarp and seed, resulting in environmental pollution and resource wastage. The present study was performed to systematically evaluate functional components, i.e., polyphenols (phenolics and flavonoids) and alkaloids, in longan byproducts and their bioactivities, including antioxidant activities, nitrite scavenging activities in simulated gastric fluid and anti-hyperglycemic activities in vitro. Total phenolic and total flavonoid contents in pericarp were slightly higher than those in seeds, but seeds possessed higher alkaloid content than pericarp. Four polyphenolic substances, i.e., gallic acid, ethyl gallate, corilagin and ellagic acid, were identified and quantified using high-performance liquid chromatography. Among these polyphenolic components, corilagin was the major one in both pericarp and seed. Alkaloid extract in seed showed the highest DPPH radical scavenging activity and oxygen radical absorbance capacity. Nitrite scavenging activities were improved with extract concentration and reaction time increasing. Flavonoids in seed and alkaloids in pericarp had potential to be developed as anti-hyperglycemic agents. The research result was a good reference for exploring longan byproducts into various valuable health-care products.

scholarly journals AN IN VITRO STUDY OF ANTIOXIDANT CAPACITY AND RADICAL SCAVENGING EFFECT OF SPINACIA OLERACEA LEAF EXTRACT

2018 ◽  
Vol 11 (11) ◽  
pp. 324
Author(s):  
Mrinalini C Damle ◽  
Shivani K Jadhav
Keyword(s):  
Leaf Extract ◽  
Spinacia Oleracea ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Leaf Extracts

Objective: The study was carried out to evaluate the preliminary phytochemical screening and antioxidant activity of ethanolic extract of Spinacia oleracea (SO).Methods: The leaves of SO were shade dried, and the extract was prepared using solvent ethanol by Soxhlet extraction method. The preliminary phytochemical screening was carried out on the leaf extract of the plant. The total phenolic content and total flavonoids were estimated using Folin- Ciocalteu’s and aluminum chloride reagents, respectively. Antioxidant activities were studied using 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, hydrogen radical, lipid peroxidation, and phosphomolybdenum radical scavenging assays.Results: The preliminary phytochemical analysis revealed the presence of bioactive constituents such as phenols, alkaloids, flavonoids, saponins, and glycosides. As SO is a rich source of different bioactive component, it contains a considerable amount of flavonoids and phenols. The different antioxidant assays proved that spinach is one of the best antioxidants with its ability to scavenge different radicals that generate oxidative stress.Conclusion: The observed activity may be associated with bioactive components such as phenols and flavonoids present in the leaf extracts and could have greater importance as nootropic plant in oxidative stress-related degenerative diseases such as Alzheimer and dementia.

Comparative Studies on the Phytochemical Constituents and in vitro Antioxidant Activities of Methanol and Ethyl Acetate Leaf Extracts of Talium Trangulare

2021 ◽  
Vol 7 (1) ◽  
pp. 18-23
Author(s):  
Oyedotun Oyeleke ◽  
Mutiat Bamigboye ◽  
Boyede Olowookere ◽  
Kehinde Alabi ◽  
Samuel Oladele ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Comparative Studies ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Phytochemical Content ◽  
Phytochemical Constituents

Medicinal herbs have been known for their use as an alternative medicine in the management and treatment of different categories of diseases. The present study was designed to assess the phytochemical constituents present in methanol and ethylacetate leaves extract of Talium trangulare and its antioxidant potentials against free radicals comparatively. The functional groups of the phytochemicals were carried out using FTIR techniques while the phytochemical component of the extract was determined by standard methods. The comparative studies of antioxidant abilities of methanol and ethylacetate leaves extracts of Talium trangulare were evaluated by various antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide, hydroxyl radicals, scavenging activities. These different antioxidant activities were compared with standard antioxidants compounds such as ascorbic acid and Gallic acid. The total phenolic content in ethylacetate extract is higher than what was obtained from methanol extract of Talium trangulare, however, methanol extract has higher tannins and flavonoids content. The methanol extract was found to have demonstrated high reduction capability and powerful free radical scavenging, especially against DPPH radical and hydrogen peroxide as compared with the ethylacetate leaves extract. The results that was obtained in the present study clearly established the antioxidant potency of both ethylacetate and methanol leaf extracts of Talium trangulare. The methanol extract shows better antioxidant activities and more flavonoids and tannin than the ethylacetate extract. In conclusion, the methanol leaf extract of Talium trangulare has comparatively better in vitro antioxidant potentials with corresponding phytochemical content than ethylacetate extract.

scholarly journals Optimization of sunflower head pectin extraction by ammonium oxalate and the effect of drying conditions on properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xuemei Ma ◽  
Jiayi Yu ◽  
Jing Jing ◽  
Qian Zhao ◽  
Liyong Ren ◽  
...  
Keyword(s):  
High Performance ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Ammonium Oxalate ◽  
Extraction Process ◽  
Size Exclusion ◽  
Pharmaceutical Industries

AbstractPectin is a kind of natural and complex carbohydrates which is extensively used in food, chemical, cosmetic, and pharmaceutical industries. Fresh sunflower (Helianthus annuus L.) heads were utilized as a novel source of pectin extracted by ammonium oxalate. The conditions of the extraction process were optimized implementing the response surface methodology. Under optimal extraction parameters (extraction time 1.34 h, liquid–solid ratio 15:1 mL/g, ammonium oxalate concentration 0.76% (w/v)), the maximum experimental yield was 7.36%. The effect of spray-drying and freeze-drying on the physiochemical properties, structural characteristics, and antioxidant activities was investigated by FT-IR spectroscopy, high performance size exclusion chromatography, and X-ray diffraction. The results showed freeze-drying lead to decrease in galacturonic acid (GalA) content (76.2%), molecular weight (Mw 316 kDa), and crystallinity. The antioxidant activities of pectin were investigated utilizing the in-vitro DPPH and ABTS radical-scavenging systems. This study provided a novel and efficient extraction method of sunflower pectin, and confirmed that different drying processes had an effect on the structure and properties of pectin.

Functionalized silver nanoparticles synthesized from marine algae: In vitro Evaluation of Antibacterial Potential

2021 ◽  
Vol 16 (8) ◽  
pp. 38-49
Author(s):  
Siva Kumar Kandula ◽  
Satyanarayana Swamy Cheekatla ◽  
Venkata Satya Mahesh Kumar Metta ◽  
Venkata Rajagopal Saladi
Keyword(s):  
Silver Nanoparticles ◽  
Antioxidant Activities ◽  
Algal Species ◽  
Synthesis Method ◽  
Radical Scavenging ◽  
Visible Spectrum ◽  
Total Phenolic ◽  
Face Centered Cubic ◽  
Frap Assay

Natural antioxidants, in particular phenolic derivatives, are used efficiently to combat against oxidative induced tissue damages. The objective of the study is to determine the antioxidant potential of methanolic extracts obtained from eight marine algal species (Enteromorpha compressa, Chaetomorpha antennina, Caulerpa racemosa, Caulerpa taxifolia, Sargassum vulgare, Padina tetrastromatica, Amphiroa fragilissima and Gracilaria corticata) by assessing their total phenolic content, DPPH scavenging assay, FRAP assay, H2O2 radical and superoxide radical scavenging activities. Among them, P.tetrastromatica, S.vulgare, E.compressa, C.taxifolia display significant antioxidant activities. Further, the aqueous extracts of these four algae are used for bioreduction of silver nitrate to silver nanoparticles (AgNPs) by green synthesis method at room temperature. UV-Visible spectrum revealed the surface plasmon resonance at 430 and 440nm. The characterizations of AgNPs by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies revealed the bioreduction and capping of AgNPs. XRD analysis elucidated the synthesized nanoparticles having face centered cubic crystalline geometry, with a mean size of 17 nm. The nanoparticles have better antimicrobial activity against cocci shaped than the rod shaped bacteria. The minimum inhibitory concentration and minimum bactericidal concentration exhibit more activity against S.aureus and B.cereus rather than E.coli.

scholarly journals Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker
Keyword(s):  
Ascorbic Acid ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

scholarly journals Phytochemical contents and antioxidant activities of fern, Asplenium ceterach L. in different altitudes

2019 ◽  
Vol 48 (2) ◽  
pp. 315-320
Author(s):  
Asuman Karadeniz Pekgöz ◽  
Ilker Çinbilgel
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ecological Factors ◽  
Total Phenolic ◽  
Plant Chemical ◽  
Dpph Radical Scavenging ◽  
Phytochemical Contents ◽  
Different Altitudes

Phytochemical contents and antioxidant activities of fern (Asplenium ceterach L.) distributed in different altitudes (22 stations) were compared and the relationships between altitude and plant chemical contents were studied. The highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was found in 22nd station (IC50 = 47.91 μg/ml) and the highest total phenolic content was found in 9th station (110.62 μgGAE/ml) whereas the maximum total flavonoid content was found in 20th station (232.67 μgCE/ml). High performance liquid chromatography (HPLC) analysis indicates that the maximum pterosin b (0.235 μg/ml), catechin (2.756 μg/ml) and quercetin (0.207 μg/ml) values were found in 21th station whereas the maximum chlorogenic acid (17.718 μg/ml) was obtained in 9th and caffeic acid (6,598 μg/ml) in 13th stations. It was observed that altitude is not potent alone, but it can be a factor in the occurrence of other ecological factors like soil properties, water, humidity, light and temperature.

scholarly journals Quantification of rutin and quercetin by HPTLC/HPLC and in vitro immunomodulatory and anticancer activities of Capparis moonii fruits extracts

2017 ◽  
Vol 7 (1) ◽  
pp. 153
Author(s):  
Gaurav Mahesh Doshi ◽  
Manjushree Kundalik Pawar ◽  
Kajal Haribhai Chavda
Keyword(s):  
Gallic Acid ◽  
Cell Lines ◽  
Antibody Titre ◽  
High Performance ◽  
Assay Method ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid ◽  
Rutin And Quercetin

Background: The current research was undertaken on dried fruits of Capparis moonii to screen its potential for immunomodulatory and cancer indications with identification of phytoconstituents by chromatographic techniques.Methods: Methanolic (MECN), hydro-methanolic (HMECN) and aqueous extracts (AQCN) of Capparis moonii were subjected to high performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) after studying the total phenolic and flavonoid content by using rutin and gallic acid as standards respectively as well as undertaking powder characteristics and preliminary phytochemical screening. Immunomodulatory activities covered were hemagglutination antibody titre and delayed-type hypersensitivity reaction with the aid of sheep red blood cells (0.5×109) as antigens.  The extracts were studied for antioxidant potential. Anticancer prospects were focusing on in vitro cell lines screening (MCF 7 and HCT 15) by Sulforhodamine B assay method and potato disc assay.Results: The total phenolic and flavonoid content of MECM, HMECM and AQCM fruits extracts were found to be 0.20, 0.11 and 0.47 mg of gallic acid/g and 78.3, 18.8 and 64.4 mg of rutin/g respectively. Rutin and quercetin were confirmed by HPTLC and HPLC showing well resolved peaks. IC50 values in antioxidant studies were found to be significant with all the extracts. Significant immunomodulatory effect was noticed at 200mg/kg in both models (high antibody titre levels and decrease paw volume after 48 h). Unsatisfactory results were observed with selected cell lines and disc assay.Conclusions: Thus, selected fruits may probably have immunomodulatory potential due to presence of flavonols (rutin and quercetin).

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