scholarly journals Needle Transportable Semi-Automatic Hair Follicle Implanter and Image-Based Hair Density Estimation for Advanced Hair Transplantation Surgery

2020 ◽  
Vol 10 (11) ◽  
pp. 4046
Author(s):  
Tae Wuk Bae ◽  
Yong Chul Jung ◽  
Kyu Hyung Kim
Keyword(s):  
Hair Follicle ◽  
Musculoskeletal Disorders ◽  
Density Estimation ◽  
Hair Follicles ◽  
Pain Tolerance ◽  
Extraction Technology ◽  
Hair Transplantation ◽  
Hair Density ◽  
Transplantation Surgery ◽  
Clinical Experiments

As the number of hair follicles transplanted in a patient is approximately 2000 or more, hair transplantation by a manual implanter requires a considerable amount of time and money and demands a great amount of patience and pain tolerance from both patients and surgeons. In addition, many surgeons frequently experience musculoskeletal disorders owing to the numerous simple repetitive motions of the shoulder and wrist during the procedure. Moreover, the results of the surgery may vary depending on the skill of the surgeon. Although the hair follicle extraction technology has advanced in recent years, the hair follicle transplantation technology still uses a simple hand-held implanter owing to the difficulty of transplanting hair follicles and hair. In this study, a needle transportable semi-automatic hair follicle implanter that can continuously transplant hair follicles is introduced to alleviate the inconvenience of the existing manual implanter. In the developed semi-automatic implanter, a plurality of needles, into which the hair follicles are inserted, are sequentially supplied from the needle supply magazine to the implanter body by the guide groove. The hair follicles in the supplied needles are transplanted to the scalp through the forward and backward movement of the needle gripper and driving motors. The developed implanter can transplant several hair follicles without any replacement, which can shorten surgery time and reduce the fatigue experienced by patients and surgeons. The effectiveness of the proposed implanter was verified through the results of animal and clinical experiments.

scholarly journals The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

2020 ◽  
Author(s):  
Gongyan Liu ◽  
Shu Li ◽  
Hongli Liu ◽  
Yanli Zhu ◽  
Liya Bai ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Signaling Pathways ◽  
Adult Life ◽  
Hair Follicles ◽  
Bmp Signaling ◽  
Skin Tissue ◽  
Follicle Development ◽  
Hair Density ◽  
Phosphorylation Level ◽  
Hair Follicle Development

Abstract Background: Hair follicles is an appendage from the vertebrate skin epithelium, and arise from the embryonic ectoderm andregenerate cyclically during adult life. Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Recent studies have reported that miRNA plays an important role in regulating hair follicle morphogenesis, proliferation, differentiation and apoptosis of hair follicle stem cells. Results: The miRNAs expression profile of the DPCs from different hair density Rex rabbits shown that 240 differentially expressed of miRNAs were screened (log 2 (HD/LD)｜>1.00 and Q-value≤0.001). Among them, the expression of ocu-miR-205-5p in low hair densities DPCs was higher than that in high hair densities, and it is highly expressed in the skin tissue of Rex rabbits ( P <0.05). ocu-miR-205 could increase cell proliferation and cell apoptosis ratio, change cell cycle process ( P <0.05), affect the genes expression of PI3K/Akt, Wnt, Notch and BMP signaling pathways in DPCs and skin tissue of Rex rabbits, inhibit the protein phosphorylation level of CTNNB1, GSK-3β and the protein expression level of noggin (NOG), promote Akt phosphorylation level ( P <0.05). There was no significant change in primary follicle density ( P >0.05), but the secondary follicle density and total follicle density ( P <0.05) were changed after ocu-miR-205-5p interfered expression, and secondary/primary ratio (S/P) in ocu-miR-205-5p interfered expression group increased at 14 days after injection ( P <0.05). Conclusion: ocu-miR-205 could promote the apoptosis of DPCs, affect PI3K/Akt, Wnt, Notch and BMP signaling pathways genes and proteins expression in DPCs and skin of Rex rabbits, promote the transformation of hair follicles from growth phase to regression and resting phase, and affect hair density of Rex rabbits.

scholarly journals Innovative modified hair follicle harvesting technique with reverse rake scalp elevator for lower occipital donor area in follicular unit extraction hair transplantation

2016 ◽  
Vol 49 (03) ◽  
pp. 390-396
Author(s):  
Chandrakant Rambhau Gharwade
Keyword(s):  
Hair Follicle ◽  
Acute Angle ◽  
Hair Follicles ◽  
Hair Transplantation ◽  
Hair Transplant ◽  
Harvesting Technique ◽  
Donor Area ◽  
Follicular Unit Extraction ◽  
Follicular Unit ◽  
Scalp Skin

ABSTRACTFollicular unit extraction (FUE) is one of the widely practiced minimally invasive follicular harvesting techniques employed during hair transplantation. FUE technique has an advantage of utilising lower occipital area and supra-auricular region as a safe donor area described by Unger, in addition to the standard occipital donor area used in strip method (follicular unit transplant). Despite its potential advantages such as rapid recovery, minimal scarring and reduced post-operative pain; its widespread acceptance is limited due to various factors in variable contribution like steeper learning curve and potentially higher follicular transection rates (FTRs). The main practical drawbacks in harvesting FUE from lower occipital donor region that lie inferior to the standard donor area, is its acute angle (10°–15°) of emergent hair from scalp skin, higher variance angle (15°–35°) between hairs below the skin and hair exit angle above the skin and comparatively loose scalp, preventing to provide stable platform for punching. Hair transplant surgeon faces difficulty in aligning and engaging the FUE punch leading to very high hair follicle transection rate, and therefore, it is not a preferred site for harvesting follicles in FUE. Authors description of modified technique using reverse rake scalp elevator helps in negating the acute angle of the hair follicles exit from scalp skin and reducing the variance angle between emergent hair and hair below the skin in lower occipital region thereby reducing FTR. Furthermore, an added advantage of reducing the overall operative time and surgeon fatigue, improve donor area healing, availability of a comparatively larger donor area which increases the confidence of the beginners. This method will be of help as it is easy to duplicate and follow by novice hair transplant surgeons and also for those who are routinely doing mega hair transplants sessions.

scholarly journals The functions of ocu-miR-205 in regulating hair follicle development of Rex rabbits

2019 ◽  
Author(s):  
Gongyan Liu ◽  
Shu Li ◽  
Hongli Liu ◽  
Yanli Zhu ◽  
Liya Bai ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Signaling Pathways ◽  
Adult Life ◽  
Hair Follicles ◽  
Bmp Signaling ◽  
Skin Tissue ◽  
Follicle Development ◽  
Hair Density ◽  
Phosphorylation Level ◽  
Hair Follicle Development

Abstract Background: Hair follicles is an appendage from the vertebrate skin epithelium, and arise from the embryonic ectoderm andregenerate cyclically during adult life. Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Recent studies have reported that miRNA plays an important role in regulating hair follicle morphogenesis, proliferation, differentiation and apoptosis of hair follicle stem cells. Results: The miRNAs expression profile of the DPCs from different hair density Rex rabbits shown that 240 differentially expressed of miRNAs were screened (log 2 (HD/LD)｜>1.00 and Q-value≤0.001). Among them, the expression of ocu-miR-205-5p in low hair densities DPCs was higher than that in high hair densities, and it is highly expressed in the skin tissue of Rex rabbits ( P <0.05). ocu-miR-205 could increase cell proliferation and cell apoptosis ratio, change cell cycle process ( P <0.05), affect the genes expression of PI3K/Akt, Wnt, Notch and BMP signaling pathways in DPCs and skin tissue of Rex rabbits, inhibit the protein phosphorylation level of CTNNB1, GSK-3β and the protein expression level of noggin (NOG), promote Akt phosphorylation level ( P <0.05). There was no significant change in primary follicle density ( P >0.05), but the secondary follicle density and total follicle density ( P <0.05) were changed after ocu-miR-205-5p interfered expression, and secondary/primary ratio (S/P) in ocu-miR-205-5p interfered expression group increased at 14 days after injection ( P <0.05). Conclusion: ocu-miR-205 could promote the apoptosis of DPCs, affect PI3K/Akt, Wnt, Notch and BMP signaling pathways genes and proteins expression in DPCs and skin of Rex rabbits, promote the transformation of hair follicles from growth phase to regression and resting phase, and affect hair density of Rex rabbits.

scholarly journals The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

2020 ◽  
Author(s):  
Gongyan Liu ◽  
Shu Li ◽  
Hongli Liu ◽  
Yanli Zhu ◽  
Liya Bai ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Signaling Pathways ◽  
Adult Life ◽  
Hair Follicles ◽  
Bmp Signaling ◽  
Skin Tissue ◽  
Follicle Development ◽  
Hair Density ◽  
Phosphorylation Level ◽  
Hair Follicle Development

Abstract Background: Hair follicles is an appendage from the vertebrate skin epithelium, and arise from the embryonic ectoderm andregenerate cyclically during adult life. Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Recent studies have reported that miRNA plays an important role in regulating hair follicle morphogenesis, proliferation, differentiation and apoptosis of hair follicle stem cells. Results: The miRNAs expression profile of the DPCs from different hair density Rex rabbits shown that 240 differentially expressed of miRNAs were screened (log 2 (HD/LD)｜>1.00 and Q-value≤0.001). Among them, the expression of ocu-miR-205-5p in low hair densities DPCs was higher than that in high hair densities, and it is highly expressed in the skin tissue of Rex rabbits ( P <0.05). ocu-miR-205 could increase cell proliferation and cell apoptosis ratio, change cell cycle process ( P <0.05), affect the genes expression of PI3K/Akt, Wnt, Notch and BMP signaling pathways in DPCs and skin tissue of Rex rabbits, inhibit the protein phosphorylation level of CTNNB1, GSK-3β and the protein expression level of noggin (NOG), promote Akt phosphorylation level ( P <0.05). There was no significant change in primary follicle density ( P >0.05), but the secondary follicle density and total follicle density ( P <0.05) were changed after ocu-miR-205-5p interfered expression, and secondary/primary ratio (S/P) in ocu-miR-205-5p interfered expression group increased at 14 days after injection ( P <0.05). Conclusion: ocu-miR-205 could promote the apoptosis of DPCs, affect PI3K/Akt, Wnt, Notch and BMP signaling pathways genes and proteins expression in DPCs and skin of Rex rabbits, promote the transformation of hair follicles from growth phase to regression and resting phase, and affect hair density of Rex rabbits.

scholarly journals The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

2020 ◽  
Author(s):  
Gongyan Liu ◽  
Shu Li ◽  
Hongli Liu ◽  
Yanli Zhu ◽  
Liya Bai ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Signaling Pathways ◽  
Adult Life ◽  
Hair Follicles ◽  
Bmp Signaling ◽  
Skin Tissue ◽  
Follicle Development ◽  
Hair Density ◽  
Phosphorylation Level ◽  
Hair Follicle Development

Abstract Background: Hair follicles is an appendage from the vertebrate skin epithelium, and arise from the embryonic ectoderm andregenerate cyclically during adult life. Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Recent studies have reported that miRNA plays an important role in regulating hair follicle morphogenesis, proliferation, differentiation and apoptosis of hair follicle stem cells. Results: The miRNAs expression profile of the DPCs from different hair density Rex rabbits shown that 240 differentially expressed of miRNAs were screened (log 2 (HD/LD)｜>1.00 and Q-value≤0.001). Among them, the expression of ocu-miR-205-5p in low hair densities DPCs was higher than that in high hair densities, and it is highly expressed in the skin tissue of Rex rabbits ( P <0.05). ocu-miR-205 could increase cell proliferation and cell apoptosis ratio, change cell cycle process ( P <0.05), affect the genes expression of PI3K/Akt, Wnt, Notch and BMP signaling pathways in DPCs and skin tissue of Rex rabbits, inhibit the protein phosphorylation level of CTNNB1, GSK-3β and the protein expression level of noggin (NOG), promote Akt phosphorylation level ( P <0.05). There was no significant change in primary follicle density ( P >0.05), but the secondary follicle density and total follicle density ( P <0.05) were changed after ocu-miR-205-5p interfered expression, and secondary/primary ratio (S/P) in ocu-miR-205-5p interfered expression group increased at 14 days after injection ( P <0.05). Conclusion: ocu-miR-205 could promote the apoptosis of DPCs, affect PI3K/Akt, Wnt, Notch and BMP signaling pathways genes and proteins expression in DPCs and skin of Rex rabbits, promote the transformation of hair follicles from growth phase to regression and resting phase, and affect hair density of Rex rabbits.

scholarly journals The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

2020 ◽  
Author(s):  
Gongyan Liu ◽  
Shu Li ◽  
Hongli Liu ◽  
Yanli Zhu ◽  
Liya Bai ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Signaling Pathways ◽  
Adult Life ◽  
Hair Follicles ◽  
Bmp Signaling ◽  
Skin Tissue ◽  
Follicle Development ◽  
Hair Density ◽  
Phosphorylation Level ◽  
Hair Follicle Development

Abstract Background: Hair follicles is an appendage from the vertebrate skin epithelium, and arise from the embryonic ectoderm andregenerate cyclically during adult life. Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Recent studies have reported that miRNA plays an important role in regulating hair follicle morphogenesis, proliferation, differentiation and apoptosis of hair follicle stem cells. Results: The miRNAs expression profile of the DPCs from different hair density Rex rabbits shown that 240 differentially expressed of miRNAs were screened (log 2 (HD/LD)｜>1.00 and Q-value≤0.001). Among them, the expression of ocu-miR-205-5p in low hair densities DPCs was higher than that in high hair densities, and it is highly expressed in the skin tissue of Rex rabbits ( P <0.05). ocu-miR-205 could increase cell proliferation and cell apoptosis ratio, change cell cycle process ( P <0.05), affect the genes expression of PI3K/Akt, Wnt, Notch and BMP signaling pathways in DPCs and skin tissue of Rex rabbits, inhibit the protein phosphorylation level of CTNNB1, GSK-3β and the protein expression level of noggin (NOG), promote Akt phosphorylation level ( P <0.05). There was no significant change in primary follicle density ( P >0.05), but the secondary follicle density and total follicle density ( P <0.05) were changed after ocu-miR-205-5p interfered expression, and secondary/primary ratio (S/P) in ocu-miR-205-5p interfered expression group increased at 14 days after injection ( P <0.05). Conclusion: ocu-miR-205 could promote the apoptosis of DPCs, affect PI3K/Akt, Wnt, Notch and BMP signaling pathways genes and proteins expression in DPCs and skin of Rex rabbits, promote the transformation of hair follicles from growth phase to regression and resting phase, and affect hair density of Rex rabbits.

scholarly journals The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

2020 ◽  
Author(s):  
Gongyan Liu ◽  
Shu Li ◽  
Hongli Liu ◽  
Yanli Zhu ◽  
Liya Bai ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Signaling Pathways ◽  
Adult Life ◽  
Hair Follicles ◽  
Bmp Signaling ◽  
Skin Tissue ◽  
Follicle Development ◽  
Hair Density ◽  
Phosphorylation Level ◽  
Hair Follicle Development

Abstract Background: Hair follicles is an appendage from the vertebrate skin epithelium, and arise from the embryonic ectoderm andregenerate cyclically during adult life. Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Recent studies have reported that miRNA plays an important role in regulating hair follicle morphogenesis, proliferation, differentiation and apoptosis of hair follicle stem cells. Results: The miRNAs expression profile of the DPCs from different hair density Rex rabbits shown that 240 differentially expressed of miRNAs were screened (log 2 (HD/LD)｜>1.00 and Q-value≤0.001). Among them, the expression of ocu-miR-205-5p in low hair densities DPCs was higher than that in high hair densities, and it is highly expressed in the skin tissue of Rex rabbits ( P <0.05). ocu-miR-205 could increase cell proliferation and cell apoptosis ratio, change cell cycle process ( P <0.05), affect the genes expression of PI3K/Akt, Wnt, Notch and BMP signaling pathways in DPCs and skin tissue of Rex rabbits, inhibit the protein phosphorylation level of CTNNB1, GSK-3β and the protein expression level of noggin (NOG), promote Akt phosphorylation level ( P <0.05). There was no significant change in primary follicle density ( P >0.05), but the secondary follicle density and total follicle density ( P <0.05) were changed after ocu-miR-205-5p interfered expression, and secondary/primary ratio (S/P) in ocu-miR-205-5p interfered expression group increased at 14 days after injection ( P <0.05). Conclusion: ocu-miR-205 could promote the apoptosis of DPCs, affect PI3K/Akt, Wnt, Notch and BMP signaling pathways genes and proteins expression in DPCs and skin of Rex rabbits, promote the transformation of hair follicles from growth phase to regression and resting phase, and affect hair density of Rex rabbits.

A method for culturing human hair follicle cells. (II). Plucked human hair follicles.

1988 ◽  
Vol 50 (2) ◽  
pp. 271-276 ◽  
Author(s):  
Ryuichiro KUWANA ◽  
Seiji ARASE ◽  
Yasushi SADAMOTO ◽  
Hideki NAKANISHI ◽  
Katsuyuki TAKEDA
Keyword(s):  
Hair Follicle ◽  
Human Hair ◽  
Hair Follicles ◽  
Follicle Cells ◽  
Human Hair Follicle

Nano-Lipidic Carriers as a Tool for Drug Targeting to the Pilosebaceous Units

2020 ◽  
Vol 26 (27) ◽  
pp. 3251-3268
Author(s):  
Shweta Ramkar ◽  
Abhishek K. Sah ◽  
Nagendra Bhuwane ◽  
Ishwari Choudhary ◽  
Narayan Hemnani ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Hair Follicle ◽  
Hair Follicles ◽  
Prolonged Release ◽  
Pilosebaceous Unit ◽  
Tissue Targeting ◽  
Bulge Region ◽  
Drug Reservoir ◽  
Fast Transport ◽  
Topical Applications

The pilosebaceous unit is the triad comprising of hair follicle, arrector pilli muscle, and sebaceous gland. Drug delivery to and through the hair follicles has garnered much attention of the researchers and the hair follicles represent an attractive target site via topical applications. They are bordered by capillaries and antigenpresenting cells, connected to the sebaceous glands and the bulge region of the hair follicle anchors the stem cells. The nano lipid carriers have the propensity to penetrate through the skin via transcellular route, intracellular route and follicular route. It has been established that nano lipid carriers have the potential for follicular drug delivery and provide some advantages over conventional pathways, including improved bioavailability, enhanced penetration depth, fast transport into the skin, tissue targeting and form a drug reservoir for prolonged release. This review describes the pilosebaceous unit (PSU) and related diseases and the recent lipid-based nanotechnology approaches for drug delivery to the follicular unit as well as related issues. Different types of nano lipid carriers, including ethosomes, liposomes, nanoparticles, solid lipid nanoparticles (SLNs), and nano lipid carriers (NLCs) have been reported for follicular drug delivery. Targeted drug delivery with nano-lipid carriers has the potential to augment the efficacy of drugs/bioactives to treat diseases of PSU. This review systematically introduces the activities of different formulations and the use of nano lipid carriers in treating PSU related disorders like alopecia, acne, and hirsutism.

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