scholarly journals Surface Modification of 3D Printed PLA/Halloysite Composite Scaffolds with Antibacterial and Osteogenic Capabilities

2020 ◽  
Vol 10 (11) ◽  
pp. 3971
Author(s):  
Yangyang Luo ◽  
Ahmed Humayun ◽  
David K. Mills
Keyword(s):  
Surface Modification ◽  
Mechanical Strength ◽  
Type I Collagen ◽  
Hydrophobic Surface ◽  
Halloysite Nanotubes ◽  
Calcium Deposition ◽  
Material Surface ◽  
Type I ◽  
3D Scaffold ◽  
3D Printed

Three-dimensional (3D) printing techniques have received considerable focus in the area of bone engineering due to its precise control in the fabrication of complex structures with customizable shapes, internal and external architectures, mechanical strength, and bioactivity. In this study, we design a new composition biomaterial consisting of polylactic acid (PLA), and halloysite nanotubes (HNTs) loaded with zinc nanoparticles (PLA+H+Zn). The hydrophobic surface of the 3D printed scaffold was coated with two layers of fetal bovine serum (FBS) on the sides and one layer of NaOH in the middle. Additionally, a layer of gentamicin was coated on the outermost layer against bacterial infection. Scaffolds were cultured in standard cell culture medium without the addition of osteogenic medium. This surface modification strategy improved material hydrophilicity and enhanced cell adhesion. Pre-osteoblasts cultured on these scaffolds differentiated into osteoblasts and proceeded to produce a type I collagen matrix and subsequent calcium deposition. The 3D printed scaffolds formed from this composition possessed high mechanical strength and showed an osteoinductive potential. Furthermore, the external coating of antibiotics not only preserved the previous osteogenic properties of the 3D scaffold but also significantly reduced bacterial growth. Our surface modification model enabled the fabrication of a material surface that was hydrophilic and antibacterial, simultaneously, with an osteogenic property. The designed PLA+H+Zn may be a viable candidate for the fabrication of customized bone implants.

scholarly journals Zinc Doped Halloysite Cooperating with Polylactic Acid for Bone Regeneration

2020 ◽  
Author(s):  
Yangyang Luo ◽  
Ahmed Humayun ◽  
David Mills
Keyword(s):  
Surface Modification ◽  
Mechanical Strength ◽  
Polylactic Acid ◽  
Type I Collagen ◽  
Halloysite Nanotubes ◽  
Calcium Deposition ◽  
Material Surface ◽  
Type I ◽  
3D Scaffold ◽  
3D Printed

Three-dimensional (3D) printing techniques have received considerable focus in the area of bone engineering due to its precise control in the fabrication of complex structures with customizable shapes, internal and external architectures, mechanical strength, and bioactivity. In this study, we design a new composition biomaterial consisting of polylactic acid (PLA), and halloysite nanotubes (HNTs) loaded with zinc nanoparticles (PLA+H+Zn). The hydrophobic surface of the 3D printed scaffold was coated with two layers of fetal bovine serum (FBS) on the sides and one layer of NaOH in the middle. Additionally, a layer of gentamicin was coated on the outermost layer against bacterial infection. Scaffolds were cultured in standard cell culture medium without the addition of osteogenic medium. This surface modification strategy improved material hydrophilicity and enhanced cell adhesion. Pre-osteoblasts cultured on these scaffolds differentiated into osteoblasts and proceeded to produce a type I collagen matrix and subsequent calcium deposition. 3D printed scaffolds formed from this composition possessed high mechanical strength and showed an osteoinductive potential. Furthermore, the external coating of antibiotics not only preserved the previous osteogenic properties of the 3D scaffold but also significantly reduced bacterial growth. Our surface modification model enabled the fabrication of a material surface that was hydrophilic and antibacterial, simultaneously, with an osteogenic property. The designed PLA+H+Zn may be a viable candidate for the fabrication of customized bone implants.

The Application of Biological Materials on the Cruciate Ligament Reconstruction of Knee-Joint in Athletic Injury

2013 ◽  
Vol 643 ◽  
pp. 25-28
Author(s):  
Kai Liu
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Surface Topology ◽  
Future Research ◽  
Material Surface ◽  
Type I ◽  
Modification Of Materials

It is Important content that to make Surface modification and surface modification and Improve the material on the surface of the cell adhesion and promotes cell proliferation to bone tissue engineering scaffolds. The role of osteoblast and support material dependent on the Material surface characteristics, Local shape, surface energy and chemical energy, which Determine how cells adsorbed onto the surface of the material and Localization of cells and cell function behavior. Therefore, the complexity of biomaterials and cell biological material surface interaction determines the biological scaffold materials for surface modification of importance. Ideal surface modification should take into consideration the surface topology, specific identification, hydrophilic and hydrophobic protein adsorption equilibrium, and other aspects of functional new tissues. At present, the most applications in surface modification of materials is type I collagen, future research will be a variety of surface modification of materials for composite materials, which will play complementary roles, as well as gene therapy and the development of nanometer materials, it will become a hot issue in the field of bone tissue engineering.

Cellular Compatibility of a New Tantalum-Niobium Scaffold Material

2020 ◽  
Author(s):  
Jianan Ouyang ◽  
Zhenhan Deng ◽  
Kang Chen ◽  
Jianyi Xiong ◽  
Ying Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Type I Collagen ◽  
Material Surface ◽  
Control Group ◽  
Type I ◽  
Scaffold Material ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Porous Tantalum ◽  
Significant Difference

Abstract [Objective] To determine the cellular compatibility of porous tantalum-niobium (Ta-Nb) material. [Method] Rabbit osteoblasts were co-cultured with porous Ta-Nb material. The cell proliferation was detected by CCK-8 method, and the cell adhesion was observed under scanning electron microscope (SEM). The expressions of type-I collagen and osteocalcin were detected by RT-PCR assay. [Results] CCK-8 detection indicated that the cell proliferation on the porous Ta-Nb material showed no difference from that of the control group (P>0.05). SEM revealed that a large amount of cells adhered onto the surface and in the pores of the material. The number of cells on the material surface increased obviously over time. RT-PCR assay showed that with the prolonging of the time of co-culture, the expression of type-I collagen was enhanced (P<0.05), while the osteocalcin expression exhibited no significant difference (P>0.05[Conclusion] Porous Ta-Nb scaffold material can be used to promote the adhesion, growth and differentiation of osteoblasts with satisfactory cellular compatibility.

Application of Tissue Engineering Techniques for Rotator Cuff Regeneration Using a Chitosan-Based Hyaluronan Hybrid Fiber Scaffold

2005 ◽  
Vol 33 (8) ◽  
pp. 1193-1201 ◽  
Author(s):  
Tadanao Funakoshi ◽  
Tokifumi Majima ◽  
Norimasa Iwasaki ◽  
Naoki Suenaga ◽  
Naohiro Sawaguchi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Rotator Cuff ◽  
Mechanical Strength ◽  
Type I Collagen ◽  
Tangent Modulus ◽  
Rotator Cuff Tears ◽  
Type I ◽  
Scaffold Materials ◽  
Novel Scaffold

Background The current surgical procedures for irreparable rotator cuff tears have considerable limitations. Tissue engineering techniques using novel scaffold materials offer potential alternatives for managing these conditions. Hypothesis A chitosan-based hyaluronan hybrid scaffold could enhance type I collagen products with seeded fibroblasts and thereby increase the mechanical strength of regenerated tendon in vivo. Study Design Controlled laboratory study. Methods The scaffolds were created from chitosan-based hyaluronan hybrid polymer fibers. Forty-eight rabbit infraspinatus tendons and their humeral insertions were removed to create defects. Each defect was covered with a fibroblast-seeded scaffold (n = 16) or a non-fibroblast-seeded scaffold (n = 16). In the other 16 shoulders, the rotator cuff defect was left free as the control. At 4 and 12 weeks after surgery, the engineered tendons were assessed by histological, immunohistochemical (n = 2), and biomechanical (n = 6) analyses. Results Type I collagen was only seen in the fibroblast-seeded scaffold and increased in the regenerated tissue. The tensile strength and tangent modulus in the fibroblast-seeded scaffold were significantly improved from 4 to 12 weeks postoperatively. The fibroblast-seeded scaffold had a significantly greater tangent modulus than did the non-fibroblast-seeded scaffold and the control at 12 weeks. Conclusion This scaffold material enhanced the production of type I collagen and led to improved mechanical strength in the regenerated tissues of the rotator cuff in vivo. Clinical Relevance Rotator cuff regeneration is feasible using this tissue engineering technique.

Curcumin loaded nano graphene oxide reinforced fish scale collagen – a 3D scaffold biomaterial for wound healing applications

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98653-98665 ◽  
Author(s):  
Tapas Mitra ◽  
Piyali Jana Manna ◽  
S. T. K. Raja ◽  
A. Gnanamani ◽  
P. P. Kundu
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Graphene Oxide ◽  
Type I Collagen ◽  
Type I ◽  
Fish Scale ◽  
3D Scaffold ◽  
Engineering Research ◽  
Nano Graphene ◽  
Tissue Engineering Research

We prepare a highly stabilized nano graphene oxide functionalized with type I collagen to make a 3D scaffold as a novel platform for better tissue engineering research..

Three-Dimensional-Printed External Scaffolds Mitigate Loss of Volume and Topography in Engineered Elastic Cartilage Constructs

Cartilage ◽  
2021 ◽  
pp. 194760352110495
Author(s):  
Xue Dong ◽  
Ishani D. Premaratne ◽  
Jaime L. Bernstein ◽  
Arash Samadi ◽  
Alexandra J. Lin ◽  
...  
Keyword(s):  
Injection Molding ◽  
Type I Collagen ◽  
Three Dimensional ◽  
Type I ◽  
Base Area ◽  
Elastic Cartilage ◽  
3D Printed ◽  
Injection Molded

Objective: A major obstacle in the clinical translation of engineered auricular scaffolds is the significant contraction and loss of topography that occur during maturation of the soft collagen-chondrocyte matrix into elastic cartilage. We hypothesized that 3-dimensional-printed, biocompatible scaffolds would “protect” maturing hydrogel constructs from contraction and loss of topography. Design: External disc-shaped and “ridged” scaffolds were designed and 3D-printed using polylactic acid (PLA). Acellular type I collagen constructs were cultured in vitro for up to 3 months. Collagen constructs seeded with bovine auricular chondrocytes (BAuCs) were prepared in 3 groups and implanted subcutaneously in vivo for 3 months: preformed discs with (“Scaffolded/S”) or without (“Naked/N”) an external scaffold and discs that were formed within an external scaffold via injection molding (“Injection Molded/SInj”). Results: The presence of an external scaffold or use of injection molding methodology did not affect the acellular construct volume or base area loss. In vivo, the presence of an external scaffold significantly improved preservation of volume and base area at 3 months compared to the naked group ( P < 0.05). Construct contraction was mitigated even further in the injection molded group, and topography of the ridged constructs was maintained with greater fidelity ( P < 0.05). Histology verified the development of mature auricular cartilage in the constructs within external scaffolds after 3 months. Conclusion: Custom-designed, 3D-printed, biocompatible external scaffolds significantly mitigate BAuC-seeded construct contraction and maintain complex topography. Further refinement and scaling of this approach in conjunction with construct fabrication utilizing injection molding may aid in the development of full-scale auricular scaffolds.

Effects of Rehydration on Dentin Strengthened by Heating or UV Irradiation

2009 ◽  
Vol 89 (2) ◽  
pp. 154-158 ◽  
Author(s):  
M. Hayashi ◽  
K. Okamura ◽  
E.V. Koychev ◽  
Y. Furuya ◽  
A. Sugeta ◽  
...  
Keyword(s):  
Mechanical Strength ◽  
Uv Irradiation ◽  
Type I Collagen ◽  
Control Group ◽  
Type I ◽  
Third Molars ◽  
Flexural Testing ◽  
Human Dentin ◽  
Dentin Collagen ◽  
Original Strength

Type I collagen, the major organic component of human dentin, plays an important role in regulating the mechanical strength of dentin. Collagen in dentin can be strengthened by heating. We hypothesized that UV irradiation could produce similar strengthening effects and might maintain the strength of dentin after rehydration. Beam-shaped dentin specimens from the crowns of human third molars were subjected to flexural testing. Flexural strengths were two and three times greater than those in the control group after 5 minutes’ UV irradiation and heating to 140°C, respectively. After 30 days of rehydration, the heated specimens reverted to their original strength, whereas the UV specimens were 69% stronger than the original. Raman spectra of dental collagen were unchanged after heating, whereas several peaks, including a C-C bond in a proline ring, were amplified by UV irradiation. It is concluded that dentin strengthened by UV irradiation retains strength after rehydration because of chemical changes in collagen.

scholarly journals 3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity

Materials ◽  
2021 ◽  
Vol 14 (23) ◽  
pp. 7255
Author(s):  
Shiva Naseri ◽  
Megan E. Cooke ◽  
Derek H. Rosenzweig ◽  
Maryam Tabrizian
Keyword(s):  
3D Printing ◽  
Type I Collagen ◽  
Three Dimensional ◽  
Raw Material ◽  
Type I ◽  
Dentinal Tubule ◽  
Human Teeth ◽  
Tooth Sensitivity ◽  
3D Printed

Tooth sensitivity is a painful and very common problem. Often stimulated by consuming hot, cold, sweet, or acidic foods, it is associated with exposed dentin microtubules that are open to dental pulp. One common treatment for tooth hypersensitivity is the application of occlusive particles to block dentin microtubules. The primary methodology currently used to test the penetration and occlusion of particles into dentin pores relies upon dentin discs cut from extracted bovine/human teeth. However, this method is limited due to low accessibility to the raw material. Thus, there is a need for an in vitro dentin model to characterize the effectiveness of occlusive agents. Three-dimensional printing technologies have emerged that make the printing of dentin-like structures possible. This study sought to develop and print a biomaterial ink that mimicked the natural composition and structure of dentin tubules. A formulation of type I collagen (Col), nanocrystalline hydroxyapatite (HAp), and alginate (Alg) was found to be suitable for the 3D printing of scaffolds. The performance of the 3D printed dentin model was compared to the natural dentin disk by image analysis via scanning electron microscopy (SEM), both pre- and post-treatment with occlusive microparticles, to evaluate the degree of dentinal tubule occlusion. The cytocompatibility of printed scaffolds was also confirmed in vitro. This is a promising biomaterial system for the 3D printing of dentin mimics.

scholarly journals Hypertrophic chondrocytes undergo further differentiation in culture

1992 ◽  
Vol 117 (2) ◽  
pp. 427-435 ◽  
Author(s):  
F Descalzi Cancedda ◽  
C Gentili ◽  
P Manduca ◽  
R Cancedda
Keyword(s):  
Collagen Synthesis ◽  
Type I Collagen ◽  
Bone Cells ◽  
Single Cells ◽  
Protein A ◽  
Culture Conditions ◽  
Hypertrophic Chondrocytes ◽  
Calcium Deposition ◽  
Type I ◽  
Collagen Secretion

Conditions have been defined for promoting growth and differentiation of hypertrophic chondrocytes obtained in culture starting from chick embryo tibiae. Hypertrophic chondrocytes, grown in suspension culture as described (Castagnola P., G. Moro, F. Descalzi Cancedda, and R. Cancedda. 1986. J. Cell Biol. 102:2310-2317), when they reached the stage of single cells, were transferred to substrate-dependent culture conditions in the presence of ascorbic acid. Cells showed a change in morphology, became more elongated and flattened, expressed alkaline phosphatase, and eventually mineralized. Type II and X collagen synthesis was halted and replaced by type I collagen synthesis. In addition the cells started to produce and to secrete in large amount a protein with an apparent molecular mass of 82 KD in reducing conditions and 63 KD in unreducing conditions. This protein is soluble in acidic solutions, does not contain collagenous domains, and is glycosylated. The Ch21 protein, a marker of hypertrophic chondrocytes and bone cells, was synthesized throughout the culture. We have defined this additional differentiation stage as an osteoblast-like stage. Calcium deposition in the extracellular matrix occurred regardless of the addition of beta glycerophosphate to the culture medium. Comparable results were obtained both when the cells were plated at low density and when they were already at confluence and maintained in culture without passaging up to 50 d. When retinoic acid was added to the hypertrophic chondrocyte culture between day 1 and day 5 the maturation of the cells to the osteoblast-like stage was highly accelerated. The switch in the collagen secretion was already observed after 2 d and the production of the 63-kD protein after 3 d. Mineralization was observed after 15-20 d.

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