scholarly journals Biological Control of Phototrophic Biofilms in a Show Cave: The Case of Nerja Cave

2020 ◽  
Vol 10 (10) ◽  
pp. 3448
Author(s):  
Valme Jurado ◽  
Yolanda del Rosal ◽  
Jose Gonzalez-Pimentel ◽  
Bernardo Hermosin ◽  
Cesareo Saiz-Jimenez

Cyanobacteria and microalgae are usually found in speleothems, rocks and walls of show caves exposed to artificial lighting. These microorganisms develop as biofilms coating the mineral surfaces and producing aesthetic, physical and chemical deterioration. A wide number of physical, chemical and environmental-friendly methods have been used for controlling the biofilms with different results. Natural biological control has been suggested by some authors as a theoretical approach but without direct evidence or application. Here we report the finding of a natural biological control of phototrophic biofilms on the speleothems of Nerja Cave, Malaga, Spain. The formation of plaques or spots where the phototrophic microorganisms disappeared can be assumed on the basis of processes of predation of bacteria, amoebas and some other organisms on the phototrophic biofilms. This study aims at investigating the potentialities of the biological control of phototrophic biofilms in caves, but the originality of these data should be confirmed in future studies with a larger number of biofilm samples in different ecological scenarios.

2021 ◽  
pp. 0958305X2198988
Author(s):  
Nur Syakirah Rabiha Rosman ◽  
Noor Aniza Harun ◽  
Izwandy Idris ◽  
Wan Iryani Wan Ismail

The emergence of technology to produce nanoparticles (1 nm – 100 nm in size) has drawn significant researchers’ interests. Nanoparticles can boost the antimicrobial, catalytic, optical, and electrical conductivity properties, which cannot be achieved by their corresponding bulk. Among other noble metal nanoparticles, silver nanoparticles (AgNPs) have attained a special emphasis in the industry due to their superior physical, chemical, and biological properties, closely linked to their shapes, sizes, and morphologies. Proper knowledge of these NPs is essential to maximise the potential of biosynthesised AgNPs in various applications while mitigating risks to humans and the environment. This paper aims to critically review the global consumption of AgNPs and compare the AgNPs synthesis between conventional methods (physical and chemical) and current trend method (biological). Related work, advantages, and drawbacks are also highlighted. Pertinently, this review extensively discusses the current application of AgNPs in various fields. Lastly, the challenges and prospects of biosynthesised AgNPs, including application safety, oxidation, and stability, commercialisation, and sustainability of resources towards a green environment, were discussed.


Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 471
Author(s):  
Angelica M. Reddy ◽  
Paul D. Pratt ◽  
Brenda J. Grewell ◽  
Nathan E. Harms ◽  
Ximena Cibils-Stewart ◽  
...  

Exotic water primroses (Ludwigia spp.) are aggressive invaders in aquatic ecosystems worldwide. To date, management of exotic Ludwigia spp. has been limited to physical and chemical control methods. Biological control provides an alternative approach for the management of invasive Ludwigia spp. but little is known regarding the natural enemies of these exotic plants. Herein the biology and host range of Lysathia flavipes (Boheman), a herbivorous beetle associated with Ludwigia spp. in Argentina and Uruguay, was studied to determine its suitability as a biocontrol agent for multiple closely related target weeds in the USA. The beetle matures from egg to adult in 19.9 ± 1.4 days at 25 °C; females lived 86.3 ± 35.6 days and laid 1510.6 ± 543.4 eggs over their lifespans. No-choice development and oviposition tests were conducted using four Ludwigia species and seven native plant species. Lysathia flavipes showed little discrimination between plant species: larvae aggressively fed and completed development, and the resulting females (F1 generation) oviposited viable eggs on most plant species regardless of origin. These results indicate that L. flavipes is not sufficiently host-specific for further consideration as a biocontrol agent of exotic Ludwigia spp. in the USA and further testing is not warranted.


Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 104
Author(s):  
Ferenc Peles ◽  
Péter Sipos ◽  
Szilvia Kovács ◽  
Zoltán Győri ◽  
István Pócsi ◽  
...  

Aflatoxins (AFs) are toxic secondary metabolites produced mostly by Aspergillus species. AF contamination entering the feed and food chain has been a crucial long-term issue for veterinarians, medicals, agroindustry experts, and researchers working in this field. Although different (physical, chemical, and biological) technologies have been developed, tested, and employed to mitigate the detrimental effects of mycotoxins, including AFs, universal methods are still not available to reduce AF levels in feed and food in the last decades. Possible biological control by bacteria, yeasts, and fungi, their excretes, the role of the ruminal degradation, pre-harvest biocontrol by competitive exclusion or biofungicides, and post-harvest technologies and practices based on biological agents currently used to alleviate the toxic effects of AFs are collected in this review. Pre-harvest biocontrol technologies can give us the greatest opportunity to reduce AF production on the spot. Together with post-harvest applications of bacteria or fungal cultures, these technologies can help us strictly reduce AF contamination without synthetic chemicals.


AGROINTEK ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 82
Author(s):  
Asnani Asnani ◽  
Abdul Rahim ◽  
Ifall Ifall

<em>This work deals with the effect of banana (sweet plaintain or ‘kepok’ variety) hump flour substituted to wheat flour in the making of noodle, on physical, chemical, and sensory characteristics of noodle.  A completely randomized was employed, with three replicates. Sensory analysis was conducted according to randomized group design. Four proportions of banana hump to wheat flour (10:90, 20:80, 30:70, and 40:60, weight basis) was studied. Results showed that the best physical and chemical characteristics was shown by noodle made from 10:90 proportion of banana hump to wheat flour, with 4.59% water absorption, 0.57% cooking loss, 10.37% water content, 34.21% starch content. However, result of sensory analysis suggested that the most liked noodle was that made from flour proportion of 20:80 with ‘like’ for colour, ‘like very much’ for aroma, ‘like’ for texture, ‘like’ for taste, and ‘like’ for overall preference</em>


Author(s):  
T. Prilipko ◽  
R. Yakubash

The results of the study of the basic physical, chemical, microbiological and hydrochemical characteristics of water ponds for growing freshwater fish. Data analysis hydrochemical regime studied ponds throughout the observation period showed that it was relatively stable fluctuated within small and do not exceed the maximum allowable substances in water. PH of water management ponds research ranged 6.1 – 7.5. Nitrites and nitrates were also in small concentrations that do not exceed regulatory and amounted to 0.1 mg N/l and 1.1 mg N/l. Established in the summer studied water samples contained more E.coli, than in the spring and autumn months and had a circle–titer less than 0.1 in 18.3% of cases. Indicator KMAFAnM water was almost 13 times higher in summer compared to spring and autumn. KilkistE.coli fall in surface water was on average within 1 – 3 CFU / cm3, and in the summer 6– 9. In the bottom water kilkistE.coli autumn was on average within 0 – 1 CFU / cm3, and in the summer 5 – 9 cfu / cm3. Indicator Value KMAFAnM in the mud was higher than the value in the bottom water fall nearly 210 times in the summer – in 417 times.


2018 ◽  
Vol 28 (4) ◽  
pp. 263-270
Author(s):  
Anita Dwi Puspitasari ◽  
Dewi Andini Kunti Mulangsri ◽  
Herlina Herlina

Sunlight has many health benefits, but high sun exposure can also cause skin problems ranging from redness, inflammation, and the worst is triggering the appearance of skin cancer. One way to protect the skin from the sun is by using a sunscreen. Kersen Leaf (Muntingia calabura L.) has a high total flavonoids and total phenolic so that it can be used as a natural active ingredient for making sunscreen creams. The purpose of this study was to formulate and evaluate sunscreen cream preparations with variations in the concentration of ethanol extract of Kersen Leaf  Muntingia calabura L.) and to know the SPF value. Extract of kersen leaves (Muntingia calabura L.) using the maceration method with 70% ethanol. The extract was concentrated using a rotary evaporator. Four formulas were made with variations in the concentration of ethanol extract of kersen leaves. Formula 1 (cream base) without ethanolic extract of kersen leaves; formula 2 with ethanol extract of 1 gram kersen leaves; formula 3 with ethanol extract of 2 grams kersen leaves and formula 4 with ethanol extract of 3 grams kersen leaves. The four formulas were tested for physical, chemical and SPF values. Testing the SPF value using spectrophotometric method. For sticky power does not meet standard because it is less than 4 seconds. The Formula 1 SPF value is 0.1149; formula 2 is 7.6574 (extra protection); formula 3 is 13.7847 (maximum protection); and formula 4 is 19.0871 (ultra protection). The greater the concentration of ethanol extract of kersen leaves the greater the SPF value. From the results of the study, it was found that the four formulas fulfilled the requirements of physical and chemical characteristics, namely organoleptic, homogeneity, distribution, and viscosity, and had a significant SPF values, namely formula 2, 3 and 4. Abstrak Sinar matahari memiliki banyak manfaat bagi kesehatan, namun paparan sinar matahari yang tinggi juga dapat menyebabkan masalah kulit mulai dari kemerahan, peradangan, dan yang paling buruk adalah memicu munculnya kanker kulit. Salah satu cara untuk melindungi kulit dari sinar matahari yaitu dengan menggunakan tabir surya. Daun kersen (Muntingia calabura L.) mempunyai kandungan flavonoid total dan fenolik total yang tinggi sehingga dapat dimanfaatkan sebagai bahan aktif alami untuk pembuatan krim tabir surya. Tujuan penelitian ini untuk melakukan formulasi dan evaluasi sediaan krim tabir surya dengan variasi konsentrasi ekstrak etanol daun kersen (Muntingia calabura L.) serta mengetahui nilai SPF-nya. Ekstraksi daun kersen menggunakan metode maserasi dengan pelarut etanol 70%. Ekstrak dipekatkan menggunakan rotary evaporator. Dibuat empat formula dengan variasi konsentrasi ekstrak etanol daun kersen. Formula 1 (basis krim) tanpa ekstrak etanol daun kersen; formula 2 dengan ekstrak etanol daun kersen 1 gram; formula 3 dengan ekstrak etanol daun kersen 2 gram; dan formula 4 dengan ekstrak etanol daun kersen 3 gram. Keempat formula diuji karakteristik sifat fisika, kimia dan nilai SPFnya. Pengujian nilai SPF menggunakan metode spektrofotometri. Untuk daya lekat belum memenuhi standar karena kurang dari empat detik. Nilai SPF Formula 1 sebesar 0,1149; formula 2 sebesar 7,6574 (proteksi ekstra); formula 3 sebesar 13,7847 (proteksi maksimal); dan formula 4 sebesar 19,0871 (proteksi ultra). Semakin besar konsentrasi ekstrak etanol daun kersen semakin besar nilai SPF-nya. Dari hasil penelitian diperoleh bahwa keempat formula memenuhi persyaratan karakteristik sifat fisika dan kimia yaitu organoleptis, homogenitas, daya sebar pH, dan viskositas, serta memiliki nilai SPF yang bermakna yaitu formula 2, 3, dan 4.


2020 ◽  
Vol 1 (2) ◽  
pp. 7-16
Author(s):  
Winda Amilia ◽  
Andrew Setiawan Rusdianto ◽  
Arma Dwi Novemi

The amount of mango production in Indonesia is quite high, but the quality of postharvest mangoes is still quite low. The quality of the fruit will decline due to contamination; one of the contaminants is fungi. The way to reduce the damage of postharvest products is by coating applications. The purpose of this study was to study the physical, chemical and antifungal activities of harumanis mangoes’s quality which had been given coating during storage that could cause postharvest losses of harumanis mangoes. There are 3 treatments, each of them are respectively the provision of corn based coating  6% tobacco extract, 8% tobacco extract and 10% tobacco extract. The physical and chemical properties of the antifungal coating of tobacco extract made from corn coating for post-harvest damage on harumanis mangoes were obtained by weight loss, texture, colour, respiration rate, vitamin C and total dissolved solid. Preventing coating can prevent damage after harvest and protect the harumanis mango; therefore the quality of the mangoes can be maintained. The best results from the priority with the largest diameter inhibition zone were given corn starch 10% tobacco extract. Then the higher the concentration of extract used, the greater the diameter of the inhibition zone obtained. Based on all the tests performed (physical, chemical, and antifungal) the best treatment from the treatment was obtained that consisted of mangoes with antifungal layers of corn starch 10% tobacco extract. Because the P3 obtained the best results in maintaining physical, chemical content and fungi for 15 days.


Author(s):  
Ramakoteswara Rao N ◽  
Kranthi kiran Reddy E ◽  
Leena Gahane ◽  
SV Ranganayakulu

Nano technology is the multi disciplinary science and technology, which has emerged as new science exploiting specific phenomena and direct manipulation of materials on nanoscale. Nanotechnology deals with the physical, chemical, and biological properties of structures and their parts at nanoscale dimensions. It's established on the concept by creating functional structures by controlling corpuscles and molecules on a one-by-one basis by different physical and chemical synthesis methods. Developments in materials science and, nano biotechnology is especially forestalled to provide elevates in dental sciences and initiations in oral health-related diagnostic and therapeutical methods. Keywords: Nano Science, dentistry, Nanocomposite, Nanorobots, Nanomaterials.


2020 ◽  
Vol 118 (2) ◽  
pp. e2007051117
Author(s):  
Eric C. Dunham ◽  
John E. Dore ◽  
Mark L. Skidmore ◽  
Eric E. Roden ◽  
Eric S. Boyd

Life in environments devoid of photosynthesis, such as on early Earth or in contemporary dark subsurface ecosystems, is supported by chemical energy. How, when, and where chemical nutrients released from the geosphere fuel chemosynthetic biospheres is fundamental to understanding the distribution and diversity of life, both today and in the geologic past. Hydrogen (H2) is a potent reductant that can be generated when water interacts with reactive components of mineral surfaces such as silicate radicals and ferrous iron. Such reactive mineral surfaces are continually generated by physical comminution of bedrock by glaciers. Here, we show that dissolved H2 concentrations in meltwaters from an iron and silicate mineral-rich basaltic glacial catchment were an order of magnitude higher than those from a carbonate-dominated catchment. Consistent with higher H2 abundance, sediment microbial communities from the basaltic catchment exhibited significantly shorter lag times and faster rates of net H2 oxidation and dark carbon dioxide (CO2) fixation than those from the carbonate catchment, indicating adaptation to use H2 as a reductant in basaltic catchments. An enrichment culture of basaltic sediments provided with H2, CO2, and ferric iron produced a chemolithoautotrophic population related to Rhodoferax ferrireducens with a metabolism previously thought to be restricted to (hyper)thermophiles and acidophiles. These findings point to the importance of physical and chemical weathering processes in generating nutrients that support chemosynthetic primary production. Furthermore, they show that differences in bedrock mineral composition can influence the supplies of nutrients like H2 and, in turn, the diversity, abundance, and activity of microbial inhabitants.


2021 ◽  
Vol 25 (4) ◽  
pp. 13-22
Author(s):  
Mohammed A. Rashid ◽  
◽  
Nagam O. Kariem ◽  

This study was intended to evaluate the quality of some bottled waters available in Iraqi market, by testing their physical, chemical and biological characteristics. These samples produced by four companies during the period of December- 2016 to April-2017. The selected companies had the biggest sells and were under the trade names of AlluLua, Ala, Alwafi, and Venesia. The results showed that most bottled waters samples characteristics were in compliance with Iraqi and international standards, with some superiority in water quality for the trade mark Allulua upon the other companies, but the trade mark Ala was at the end of the list of quality rank. All the tested samples showed clean bacteriological results for coliforms as pollution indicators and fecal coliforms (all tests MPN was less than 1.1). The other physical and chemical characteristics showed pH range of 6.21 ~ 7.7, TDS of 12.81 ~ 126.88 mg/L, Ca of 0.24 ~ 25.5 mg/L, Mg of 3.2 ~ 13.1 mg/L, Na of 0.92 ~ 23 mg/L, K of 0 ~ 0.39 mg/L, Hardness of 3.8 ~ 38.2 mg/L, and chloride of 3.55 ~ 35.5 mg/L.


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