scholarly journals Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering

2020 ◽  
Vol 10 (7) ◽  
pp. 2398 ◽  
Author(s):  
Kyoung-Je Jang ◽  
Sujin Kim ◽  
Sangbae Park ◽  
Woochan Kim ◽  
Yonghyun Gwon ◽  
...  
Keyword(s):  
Clustering Analysis ◽  
Maxillofacial Surgery ◽  
Promising Technique ◽  
Design Factor ◽  
Simple Method ◽  
Human Bone Tissue ◽  
Two Factors ◽  
Human Dental Pulp ◽  
Gbr Membrane ◽  
Suitable Environment

Inspired by the aligned extracellular matrix and bioceramics in human bone tissue, we investigated the relative contributions of nanotopography and equine bone powders (EBPs) with human dental pulp stem cells (DPSCs) to the osteogenesis. Both nanotopography and EBPs independently promoted the osteogenesis of DPSCs, osteogenesis was further promoted by the two factors in combination, indicating the importance of synergistic design factor of guided bone regeneration (GBR) membrane. The osteogenesis of DPSCs was affected by the polycaprolactone-based nanotopography of parallel nanogrooves as well as EBPs coating. Interestingly, both nanopattern and EBPs affected the DPSCs morphologies; nanopattern led to cell elongation and EBPs led to cell spreading and clustering. Analysis of the DPSCs-substrate interaction, DPSCs-EBPs interaction suggests that the combined environment of both factors play a crucial role in mediating osteogenic phenotype. This simple method to achieve a suitable environment for osteogenesis via nanotopography and EBPs coating modulation may be regarded as a promising technique for GBR/GTR membranes, which widely used dental and maxillofacial surgery applications.

scholarly journals In Vitro Mechanical and Biological Properties of 3D Printed Polymer Composite and β-Tricalcium Phosphate Scaffold on Human Dental Pulp Stem Cells

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3057 ◽  
Author(s):  
Shuaishuai Cao ◽  
Jonghyeuk Han ◽  
Neha Sharma ◽  
Bilal Msallem ◽  
Wonwoo Jeong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tricalcium Phosphate ◽  
Dental Pulp ◽  
Chemical Elements ◽  
Maxillofacial Surgery ◽  
3D Structure ◽  
Dental Pulp Stem Cells ◽  
Oral And Maxillofacial Surgery ◽  
Human Dental Pulp ◽  
3D Printed

3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated 3D poly (lactic-co-glycolic acid)/β-tricalcium phosphate (3D-PLGA/TCP) and 3D β-tricalcium phosphate (3D-TCP) scaffolds using two relatively distinct 3D printing (3DP) technologies. Conjunctively, we compared and investigated mechanical and biological responses on human dental pulp stem cells (hDPSCs). Physicochemical properties of the scaffolds, including pore structure, chemical elements, and compression modulus, were characterized. hDPSCs were cultured on scaffolds for subsequent investigations of biocompatibility and osteoconductivity. Our findings indicate that 3D printed PLGA/TCP and β-tricalcium phosphate (β-TCP) scaffolds possessed a highly interconnected and porous structure. 3D-TCP scaffolds exhibited better compressive strength than 3D-PLGA/TCP scaffolds, while the 3D-PLGA/TCP scaffolds revealed a flexible mechanical performance. The introduction of 3D structure and β-TCP components increased the adhesion and proliferation of hDPSCs and promoted osteogenic differentiation. In conclusion, 3D-PLGA/TCP and 3D-TCP scaffolds, with the incorporation of hDPSCs as a personalized restoration approach, has a prospective potential to repair minor and critical bone defects in oral and maxillofacial surgery, respectively.

scholarly journals Fabrication of Robust Superhydrophobic Surfaces with Dual-Curing Siloxane Resin and Controlled Dispersion of Nanoparticles

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1420
Author(s):  
Hyeran Kim ◽  
Kibeom Nam ◽  
Dong Yun Lee
Keyword(s):  
Superhydrophobic Surface ◽  
Spray Coating ◽  
Surface Hydrophobicity ◽  
Superhydrophobic Surfaces ◽  
Simple Method ◽  
Coated Materials ◽  
Two Factors ◽  
Coating Method ◽  
Dual Curing ◽  
Dispersion Of Nanoparticles

We developed a simple method for the fabrication of superhydrophobic surfaces on various substrates using spray coating. The fabrication method started with the blending of a modified hydrophobic siloxane binder, silica nanoparticles, and a volatile solvent by sonication. The mixture was spray-coated on various surfaces such as slide glass, paper, metal and fabric, forming a rough surface comprising silica particles dispersed in a hydrophobic binder. Surface hydrophobicity was affected by the surface energy of the binder and the degree of roughness. Therefore, we realized a superhydrophobic surface by controlling these two factors. The hydrophobicity of the siloxane binder was determined by the treatment of fluorine silane; the roughness was controlled by the amount of coated materials and sonication time. Thus, using the spray coating method, we obtained a superhydrophobic surface that was mechanically durable, thermally stable, and chemically resistant.

scholarly journals PENENTUAN DOSIS FORMULASI PUPUK ORGANIK UNTUK EFISIENSI PENGGUNAAN PHONSKA PADA TANAMAN JAGUNG MANIS (Zea mays sacharata Sturt.)

EUGENIA ◽  
2019 ◽  
Vol 25 (2) ◽  
Author(s):  
A. G. Tulungen ◽  
P. Tumewu ◽  
M. Montolalu ◽  
John L. Rantung ◽  
S. Tulung
Keyword(s):  
Plant Height ◽  
Sweet Corn ◽  
Block Design ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Design Factor ◽  
Randomized Block Design ◽  
Two Factors ◽  
Corn Plants ◽  
Physical And Chemical

The use of organic fertilizer provides soil with macronutrients, micronutrients, and organic acids which improve the long-term physical, chemical, and biological fertility of the soil. This research aims to: 1) Increase physical and chemical soil fertility, 2) Acquire the interaction between organic fertilizer and Ponska fertilizer dosage on sweet corn crops, 3) Acquire the most efficient dosage of PONSKA  fertilizer on sweet corn, and 4) disseminate research results through journal articles and directly to farmers.  This research will increase the efficiency of NPK Ponska chemical fertilizers by utilizing waste from around the farm and lakes to formulate organic fertilizer to apply on sweet corn crops and as a reference to teaching material. Factorial research with two factors was designed using a randomized block design. Factor I (A) = Organic fertilizer formulation, comprising of A1 = 20 ton/ha, A2 = 30 ton/ha, and A3 = 40 ton/ha.  Factor II (B) =  Phonska fertilizer,  comprising of B1 = 25 % Ponska, and B2 = 50% Ponska. Every treatment was replicated four times, yielding 24 research plots. Response variables observed were: Plant height, number of leaves, dry weight of the plant. Data were analyzed using ANOVA, followed by Tukey's honest significance difference test (alpha = 0.05) if significant differences were found. Results showed that the interaction between organic and Phonska fertilizer formulations had significant effects on plant height. A dosage of 40 ton/ha of organic fertilizer can decrease the use of phonska fertilizer by 75% based on the height of sweet corn plants.

scholarly journals Effect of rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation

2022 ◽  
Vol 951 (1) ◽  
pp. 012013
Author(s):  
A Rosmalia ◽  
Astriani ◽  
W P Sahroni ◽  
I G Permana ◽  
Despal
Keyword(s):  
Block Design ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Design Factor ◽  
Rumen Ph ◽  
Randomized Block Design ◽  
Two Factors ◽  
Total Bacteria ◽  
Degradable Protein

Abstract The availability of nitrogen and sulfur in the rumen should be synchronized to generate microbial protein synthesis. This study aimed to evaluate rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation. The experimental design was a 4 x 3 factorial randomized block design. Factor 1 was RDP levels (%CP) (R1= 60%; R2= 55%; R3= 50%; R4= 45%), and factor 2 was sulfur supplementation (S1= 0%; S2= 0.1%; S3= 0.2%). Data were analyzed with ANOVA followed by the Duncan test. The result showed that sulfur supplementation improved DMD and OMD without changes in rumen pH. NH3 concentration was influenced by RDP levels. Treatment R1 had the highest NH3 concentration (7.03 mM). There was an interaction of two factors on total VFA concentration. The combination of R1 and S2 resulted in the highest total VFA concentration than others. The total bacteria population increased with increasing RDP levels. Protozoa population was affected by RDP levels and sulfur supplementation. This study concluded that a combination of 60% RDP and 0.2% sulfur supplementation resulted in the best ration to improve ruminal fermentation and digestibility.

Paper 1: The Breathing and Combustion Requirements of the Small High-Speed Diesel Engine

1965 ◽  
Vol 180 (14) ◽  
pp. 1-9 ◽  
Author(s):  
D. Broome
Keyword(s):  
High Speed ◽  
Pressure Effects ◽  
Combustion Noise ◽  
Fuel Quality ◽  
Simple Method ◽  
Engine Operation ◽  
High Speed Engine ◽  
High Speed Diesel ◽  
Two Factors ◽  
Combustion Phase

The use of small diesel engines in light highway vehicles has increased greatly in the last few years, and such engines exhibit some specialized characteristics. The performance requirements of these engines are outlined, and their dependence on the overall air consumption of the engine is emphasized. The latter is defined as the product of the breathing and combustion phases of the engine operation. For the breathing phase, the factors controlling the volumetric efficiency arc examined and the relative importance of heating and pressure effects estimated from the results of tests on a number of engines. The scope for further improvement is discussed in relation to these two factors, and the choice of valve timing. For the combustion phase, the requirements of the high-speed engine are listed and briefly discussed under eight headings, namely, the limiting air utilization; fuel consumption; flexibility of operation over the speed range; ease of starting; intensity of combustion noise; sensitivity to fuel quality; overall reliability; and ease of design and manufacture. The relative merits of direct and indirect injection systems for this duty are then examined with some reference to recent experimental work, and directions in which improvements are possible and desirable are indicated. On considering techniques which are useful in chamber development, a simple method of assessing the airflow pattern within indirect chamber layouts is described and illustrated by reference to test sequences obtained on two different combustion chamber forms. Some general notes on methods of expediting firing tests of new chamber systems are given.

A SIMPLE METHOD OF SURGICAL DRAPING USING A STRING AND BARS IN MAXILLOFACIAL SURGERY

2005 ◽  
Vol 116 (4) ◽  
pp. 1174-1175
Author(s):  
Byung Min Yun ◽  
Jang Hyun Lee ◽  
Sang Kue Kang ◽  
Min Sung Tak
Keyword(s):  
Maxillofacial Surgery ◽  
Simple Method

A simple way for producing holographic filters suitable for image improvement

1978 ◽  
Vol 36 (1) ◽  
pp. 226-227
Author(s):  
K.-H. Herrmann ◽  
E. Reuber ◽  
P. Schiske
Keyword(s):  
Transfer Functions ◽  
Diffraction Order ◽  
Lateral Position ◽  
Simple Method ◽  
Spatial Frequencies ◽  
Resolution Electron ◽  
Wiener Filters ◽  
Image Improvement ◽  
Optical Reconstruction ◽  
Set Up

Aposteriori deblurring of high resolution electron micrographs of weak phase objects can be performed by holographic filters [1,2] which are arranged in the Fourier domain of a light-optical reconstruction set-up. According to the diffraction efficiency and the lateral position of the grating structure, the filters permit adjustment of the amplitudes and phases of the spatial frequencies in the image which is obtained in the first diffraction order.In the case of bright field imaging with axial illumination, the Contrast Transfer Functions (CTF) are oscillating, but real. For different imageforming conditions and several signal-to-noise ratios an extensive set of Wiener-filters should be available. A simple method of producing such filters by only photographic and mechanical means will be described here.A transparent master grating with 6.25 lines/mm and 160 mm diameter was produced by a high precision computer plotter. It is photographed through a rotating mask, plotted by a standard plotter.

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

EXELFS Studies of Carbon Fibers

1990 ◽  
Vol 48 (2) ◽  
pp. 72-73
Author(s):  
V. Serin ◽  
K. Hssein ◽  
G. Zanchi ◽  
J. Sévely
Keyword(s):  
Carbon Fibers ◽  
Energy Analysis ◽  
Electron Excitation ◽  
Complex Structures ◽  
High Modulus ◽  
Promising Technique ◽  
X Ray ◽  
Fine Structures ◽  
X Ray Absorption

The present developments of electron energy analysis in the microscopes by E.E.L.S. allow an accurate recording of the spectra and of their different complex structures associated with the inner shell electron excitation by the incident electrons (1). Among these structures, the Extended Energy Loss Fine Structures (EXELFS) are of particular interest. They are equivalent to the well known EXAFS oscillations in X-ray absorption spectroscopy. Due to the EELS characteristic, the Fourier analysis of EXELFS oscillations appears as a promising technique for the characterization of composite materials, the major constituents of which are low Z elements. Using EXELFS, we have developed a microstructural study of carbon fibers. This analysis concerns the carbon K edge, which appears in the spectra at 285 eV. The purpose of the paper is to compare the local short range order, determined by this way in the case of Courtauld HTS and P100 ex-polyacrylonitrile carbon fibers, which are high tensile strength (HTS) and high modulus (HM) fibers respectively.

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