scholarly journals Tyrosinase Inhibitory Activity of Soybeans Fermented with Bacillus subtilis Capable of Producing a Phenolic Glycoside, Arbutin

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1301
Author(s):  
Young Hun Jin ◽  
Ah Ran Jeon ◽  
Jae-Hyung Mah
Keyword(s):  
Inhibitory Activity ◽  
Food Industry ◽  
Early Period ◽  
Phenolic Glycoside ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibitory Activity ◽  
Rrna Sequencing ◽  
Other Substances ◽  
Skin Lightening ◽  
Bacillus Strains

The production of arbutin, an effective tyrosinase inhibitor as well as an outstanding antioxidant, by 691 Bacillus strains isolated from soybean-based foods was tested to enhance the tyrosinase inhibitory activity of soybeans via fermentation with the strains. Among the strains tested, the 5 strains capable of significantly producing arbutin were identified as B. subtilis via 16S rRNA sequencing. When soybeans were fermented with each of the selected strains, the arbutin content was highest on day 1 of fermentation and decreased thereafter. However, the tyrosinase inhibitory activity of the fermented soybeans continuously increased as fermentation progressed, whereas the activity of non-inoculated soybeans was consistently low. The results indicate that arbutin enhances the tyrosinase inhibitory activity of soybeans in the early period of fermentation, while other substances besides arbutin contribute to the activity in the later period. Consequently, soybeans fermented with arbutin-producing B. subtilis strains could be considered as a natural source of cosmeceuticals and nutricosmetics used in skin lightening and may be of interest in the food industry because they contain well-known and powerful antioxidants such as arbutin and other substances.

scholarly journals AKTIVITAS INHIBITOR TIROSINASE PADA EKSTRAK ALGA COKELAT SARGASSUM SP. AGARDH ASAL PESISIR LHOK BUBON, KABUPATEN ACEH BARAT

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Muhammad Gazali
Keyword(s):  
Inhibitory Activity ◽  
Brown Algae ◽  
Methanol Extract ◽  
Kojic Acid ◽  
Bioactive Compound ◽  
Positive Control ◽  
Marine Macroalgae ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibitory Activity ◽  
New Finding

Seaweeds are marine macro algae that can be found attach to the bottom shallow coastal waters with subsrate as attached media. There are three major groups of seaweeds namely brown algae (Phaeophyta), red (Rhodophyta) and green (Chlorophyta). Sargassum sp is one of brown algae which mostly found in the Lhok Bubon Coastal West of Aceh. Recently, exploration of marine macroalgae as bioactive sources was investigated. Seaweed contains bioactive compound which can serve as a defense from ultraviolet radiation that caused hyperpigmentation effect. The aiming of this study is to analyse the tyrosinase inhibitory activity of Sargassum sp extract from Lhok Bubon Coastal Area, West of Aceh. The results shown that the methanol extract of Sargassum sp possess phytochemical properties such as fenol, alkaloid and triterpenoid. Tyrosinase inhibitory activity of Sargassum sp methanol extract  is the best extract which can be inhibit  monophenolase with  IC50 : 1111.49 µg/ml and IC50 = 1582.31 µg/ml in diphenolase pathway with kojic acid as positive control. Moreover, etyl asetate and n-hexane extract have no activity of tyrosinase inhibitor. Therefore, new finding of tyrosinase inhibitor agent from marine macroalgae Sargassum sp give the fruitfull information for cosmeceutical industry.Keywords : Lhok Bubon, Brown Algae, Sargassum sp, Tyrosinase Inhibitor

scholarly journals Tyrosinase Inhibitor and Antioxidant Activity of Seaweed Powder from Fresh and Dried Sargassum plagyophyllum

2018 ◽  
Vol 20 (3) ◽  
pp. 488 ◽  
Author(s):  
Ayun Erwina Arifianti ◽  
Effionora Anwar ◽  
Nurjanah Nurjanah
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Active Substance ◽  
Inhibitory Activity ◽  
Kojic Acid ◽  
Radical Scavenging ◽  
Tyrosinase Inhibitor ◽  
Optimal Result ◽  
Ic50 Values ◽  
Skin Lightening

<p>Sargassum plagyophyllum from Sargassaceae family contains various bioactive compounds, namely<br />phlorotannin which is reported as an antioxidant and tyrosinase inhibitor. Tyrosinase inhibitor and<br />antioxidant activity from seaweed powder that obtained from seaweed’s slurry have not been reported. Thus,<br />this study was aimed to obtain the best seaweed slurry and powder from S. plagyophyllum based on tyrosinase<br />inhibitor and antioxidant activity, so it can be used as active substance in skin lightening cosmetic formula.<br />S. plagyophyllum which prepared fresh and dried was processed into seaweed slurry and lyophilization<br />to form powder. Antioxidant activity which was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH)<br />radical scavenging method found the IC50 values of ascorbic acid was 0.0035 mg/mL; fresh slurry 27.31<br />mg/mL; dried slurry 41.13 mg/mL; fresh powder 2.21 mg/mL; and dried powder 13.18 mg/mL. Moreover,<br />the tyrosinase inhibitory activity which was measured by enzimatic reaction with L-tyrosine as substrate<br />found IC50 values kojic acid 0.0076 mg/mL; fresh powder 4.97 mg/mL; and dried powder 11.35 mg/mL. Seaweed powder obtained from fresh ingredient is the most optimal result based on its tyrosinase inhibitor<br />and antioxidant activity, thus potential to be developed further as active substance for lightening cosmetic<br />formula.<br /><br /></p>

scholarly journals POTENSI MAKROALGA PADINA AUSTRALIS (HAUCK 1887) DI BARAT SELATAN ACEH SEBAGAI AGEN INHIBITOR TIROSINASE

2018 ◽  
Vol 5 (1) ◽  
pp. 71
Author(s):  
Mohamad Gazali ◽  
Eri Safutra ◽  
Zulfadhli Zulfadhli
Keyword(s):  
Inhibitory Activity ◽  
Brown Algae ◽  
Methanol Extract ◽  
Bioactive Compound ◽  
Positive Control ◽  
Raw Material ◽  
Marine Macroalgae ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibitory Activity ◽  
New Finding

Seaweeds are marine macro algae that can be found attach to the bottom shallow coastal waters. There are three major groups of seaweeds namely brown algae (Phaeophyta), red (Rhodophyta) and green (Chlorophyta). Padina australis is one of brown algae which mostly found in the West-South of Aceh coastal Area.  Seaweed contains bioactive compound which can serve as a defense from ultraviolet radiation that caused hyperpigmentation effect The purpose of this study is to analyse the tyrosinase inhibitory activity of P. australis extract from West-South Aceh. The results shown that the methanol extract of P. australis possess phytochemical properties such as flavonoids, tannin and saponin. tyrosinase inhibitory activity of P. australis  methanol extract  is the best extract which can be inhibit monophenolase with  IC50 : 293.520 µg/ml and IC50 = 13.571.067 µg/ml in diphenolase pathway with kojic acid as positive control. Moreover, chloroform and n-hexane extract have no activity of tyrosinase inhibitor. Therefore, new finding of tyrosinase inhibitor agent from marine macroalgae P. australis give the fruitfull information for cosmeceutical industry. P. australis parts could be complementary each other in providing the raw material of cosmetic product.

scholarly journals Tyrosinase Inhibitory Activity, 3D QSAR, and Molecular Docking Study of 2,5-Disubstituted-1,3,4-Oxadiazoles

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ramesh L. Sawant ◽  
Prashant D. Lanke ◽  
Jyoti B. Wadekar
Keyword(s):  
Enzyme Inhibition ◽  
Inhibitory Activity ◽  
3D Qsar ◽  
Docking Study ◽  
Docking Studies ◽  
Maximum Activity ◽  
Tyrosinase Inhibitor ◽  
Molecular Docking Study ◽  
Tyrosinase Inhibitory Activity ◽  
Vlife Mds

In continuation with our research program, in search of potent enzyme tyrosinase inhibitor, a series of synthesized 2,5-disubstituted 1,3,4-oxadiazoles have been evaluated for enzyme tyrosinase inhibitory activity. Subsequently, 3D QSAR and docking studies were performed to find optimum structural requirements for potent enzyme tyrosinase inhibitor from this series. The synthesized 20 compounds of 2,5-disubstituted-1,3,4-oxadiazole series were screened for mushroom tyrosinase inhibitory activity at various concentrations by enzyme inhibition assay. The percentage enzyme inhibition was calculated by recording absorbance at 492 nm with microplate reader. 3D QSAR and docking studies were performed using VLife MDS 3.5 software. In the series 2,5-disubstituted-1,3,4-oxadiazoles enzyme tyrosinase inhibitory activity was found to be dose dependent with maximum activity for compounds4c,4h,4m, and4r. 3D QSAR and docking studies revealed that more electropositive and less bulky substituents if placed on 1,3,4-oxadiazole nucleus may result in better tyrosinase inhibitory activity in the series.

Phlorotannins Derived From the Brown Alga Colpomenia bullosa as Tyrosinase Inhibitors

2021 ◽  
Vol 16 (7) ◽  
pp. 1934578X2110213
Author(s):  
Hideyuki Kurihara ◽  
Kazuki Kujira
Keyword(s):  
Inhibitory Activity ◽  
Brown Alga ◽  
Molecular Structures ◽  
Free Form ◽  
Biosynthesis Pathway ◽  
Tyrosinase Inhibitor ◽  
Melanin Biosynthesis ◽  
Tyrosinase Inhibitory Activity ◽  
Tyrosinase Inhibitors ◽  
Potential Use

Tyrosinase catalyzes hydroxylation of L-tyrosine and dehydrogenation of L-DOPA in the melanin biosynthesis pathway. Tyrosinase inhibitors have potential use as cosmetic whitening agents and for preventing seafood deterioration. In this report, tyrosinase inhibitors extracted from brown alga Colpomenia bullosa (Scytosiphonaceae, Scytosiphonales) were investigated. Inhibitory principles were isolated from the extract and identified as phlorotannins, phloroglucinol (1), diphlorethol (2), triphlorethol C (3), which have not been isolated in a free form previously, and fucophlorethol C (4). Compounds 3 and 4 have not been reported previously as tyrosinase inhibitors. Triphlorethol C (3) was the most potent tyrosinase inhibitor among the phlorotannins isolated, whereas isomeric fucophlorethol C (4) displayed the weakest inhibitory activity. The results suggest that molecular structures of phlorotannins strongly affect their tyrosinase inhibitory activity.

scholarly journals Glutathione for skin lightening for dermatologists and cosmetologists

2020 ◽  
Vol 6 (2) ◽  
pp. 284
Author(s):  
Shobhit Mohan ◽  
Lalit Mohan ◽  
Renu Sangal ◽  
Neelu Singh
Keyword(s):  
Inhibitory Activity ◽  
Clinical Evidence ◽  
Current Status ◽  
Reduced Form ◽  
Skin Whitening ◽  
Safety And Efficacy ◽  
Whitening Effect ◽  
Tyrosinase Inhibitory Activity ◽  
Wonder Drug ◽  
Skin Lightening

<p class="abstract">Glutathione is a potential antioxidant and its reduced form (GSH) has a good skin-whitening effect in humans through its tyrosinase inhibitory activity. Many physicians consider it as a Wonder drug for skin lightening and treatment of hyperpigmentation, especially with darker skin tones. Glutathione is available in topical, oral and injectable formulations. Topical and oral forms are considered to be safe. Intravenous form did not prove its safety and efficacy to till date. In this article, we shall review and discuss the current status of glutathione as a skin lightening agent and address the miscellaneous unanswered queries regarding the dosage, duration of use and longevity of accrued effects based on clinical evidence and recent insights into its antimelanogenic mechanism.</p>

scholarly journals Azastilbene Analogs as Tyrosinase Inhibitors: New Molecules with Depigmenting Potential

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Larissa Lavorato Lima ◽  
Rebeca Mól Lima ◽  
Annelisa Farah da Silva ◽  
Antônio Márcio Resende do Carmo ◽  
Adilson David da Silva ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibition ◽  
Tyrosinase Inhibitory Activity ◽  
Qualitative And Quantitative ◽  
Phenolic Ring ◽  
Tyrosinase Inhibitors ◽  
Resveratrol Analogs ◽  
Better Than

This research has been an effort to develop synthetic resveratrol analogs in order to improve the depigmenting potential of natural resveratrol. Six resveratrol analogs were synthesized and tested for tyrosinase inhibitory activityin vitro, by qualitative and quantitative steps. The results showed the analogCas being the most powerful tyrosinase inhibitor (IA50= 65.67 ± 0.60 μg/mL), followed by the analogsB,E,F,A, andD, respectively. The analogCpresented a tyrosinase inhibition potential better than natural resveratrol (P<0.001). The best depigmenting activity was provided by the presence of hydroxyl in the orthoposition on the second phenolic ring.

scholarly journals Identification of Mushroom and Murine Tyrosinase Inhibitors from Achillea biebersteinii Afan. Extract

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 964
Author(s):  
Marcelina Strzępek-Gomółka ◽  
Katarzyna Gaweł-Bęben ◽  
Apostolis Angelis ◽  
Beata Antosiewicz ◽  
Zuriyadda Sakipova ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Inhibitory Activity ◽  
Scientific Evidence ◽  
Extraction Procedure ◽  
Total Extract ◽  
Tyrosinase Inhibitory Activity ◽  
Dicaffeoylquinic Acid ◽  
Achillea Biebersteinii ◽  
Release Assay ◽  
Skin Lightening

Growing scientific evidence indicates that Achillea biebersteinii is a valuable source of active ingredients with potential cosmetic applications. However, the data on its composition and pharmacological properties are still insufficient. This study aims to optimize the extraction procedure of the plant material, evaluate its phytochemical composition, and compare anti-tyrosinase potential of A. biebersteinii extracts obtained by various methods. In order to identify compounds responsible for the tyrosinase inhibitory activity of A. biebersteinii, the most active anti-tyrosinase extract was fractionated by column chromatography. The fractions were examined for their skin lightening potential by mushroom and murine tyrosinase inhibitory assays and melanin release assay. HPLC-ESI-Q-TOF-MS/MS analysis of the total extract revealed the presence of several phenolic acids, flavonoids, flavonoid glucosides, and carboxylic acid. Among them, fraxetin-8-O-glucoside, quercetin-O-glucopyranose, schaftoside/isoschaftoside, gmelinin B, 1,3-dicaffeoylquinic acid (1,3-DCQA), and ferulic acid were found in the fractions with the highest skin lightening potential. Based on obtained qualitative and quantitative analysis of the fractions, it was assumed that the caffeoylquinic acid derivatives and dicaffeoylquinic acid derivatives are more likely responsible for mushroom tyrosinase inhibitory activity of A. biebersteinii extracts and fractions. Ferulic acid was proposed as the most active murine tyrosinase inhibitor, responsible also for the reduced melanin release from B16F10 murine melanoma cells.

scholarly journals Evaluation of Antioxidant, Antimicrobial, Antityrosinase and Cytotoxic Potentials of Isatis cappadocica subsp. alyssifoli as a Potent Pharmaceutical Resource

2019 ◽  
pp. 1-12 ◽  
Author(s):  
Meltem Güner ◽  
Seyda Kanbolat ◽  
Nuriye Korkmaz ◽  
Rezzan Aliyazicioglu ◽  
Mahmoud Abudayyak ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Tyrosinase Inhibitor ◽  
Antioxidant Power ◽  
Tyrosinase Inhibitory Activity ◽  
Isatis Cappadocica

Isatis species, which are endemic across most of Turkey, exhibit antibacterial, anticancer and antiviral effects. The aim of this study was to determine of antioxidant, antimicrobial, tyrosinase inhibitor, cytotoxic activities, and phenolic profile of polar extracts of Isatis cappadocica Desv. We analyzed the antioxidant properties of extracts using total phenolic content (TPC), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging activity. The phenolic composition of methanolic extract of I. cappadocica was analyzed by reverse phase high performance liquid chromatography (RP-HPLC). Extracts’ antimicrobial properties were evaluated based on the agar well diffusion technique. Tyrosinase inhibitory activity was measured colorimetrically. Methanolic extract yielded better FRAP and CUPRAC results and aqueous extract yielded better DPPH activity. Benzoic acid, and sinapic acid were detected as major phenolic compounds. Methanolic extract was particularly effective against all the bacteria investigated, apart from Yersinia pseudotuberculosis. Methanol extract was exhibited tyrosinase inhibitory activity. The methanol extract has caused to death of cells by dosage in the high concentrations cytotoxic activity on the PC-3 and 3T3 cell lines. The results showed that I. cappadocica could be used as a natural source in the food, cosmetic, and drug industries due to their rich antioxidant, antimicrobial, cytotoxic and tyrosinase inhibitor activities.

The C‐terminally shortened analogs of a hexapeptide derived from Lingzhi hydrolysate with enhanced tyrosinase‐inhibitory activity

2021 ◽  
Author(s):  
Sucheewin Krobthong ◽  
Yodying Yingchutrakul ◽  
Pawitrabhorn Samutrtai ◽  
Kiattawee Choowongkomon
Keyword(s):  
Inhibitory Activity ◽  
Tyrosinase Inhibitory Activity
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