scholarly journals Photo-Oxidative Protection of Chlorophyll a in C-Phycocyanin Aqueous Medium

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1235
Author(s):  
Ji-Eun Hong ◽  
Jae-Hyun Lim ◽  
Tae-Yoon Kim ◽  
Hwa-Yong Jang ◽  
Han-Bin Oh ◽  
...  
Keyword(s):  
Aqueous Medium ◽  
Chlorophyll A ◽  
Atmospheric Pressure Chemical Ionization ◽  
Aqueous Media ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass ◽  
Spectrometry Analysis ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical

In this study, potential protection of chlorophyll a from illumination and oxidation-induced decomposition has been examined using C-phycocyanin (C-PC) aqueous medium. Photo-oxidation resistance of chlorophyll a was monitored in various aqueous media using ultraviolet-visible spectroscopy and direct-infusion atmospheric pressure chemical ionization mass spectrometry analysis. The spectroscopy results showed that chlorophyll a in C-PC medium experienced the lowest rate of conversion to its derivatives; thus, it was demonstrated that chlorophyll a was mostly intact in the C-PC medium. Furthermore, the C-PC treated with chlorophyll a showed the lowest concentrations of malondialdehyde, and chlorophyll a in C-PC medium did not cause serious damage to human liver cells in vitro after intensive illumination. Therefore, we propose a new method of protecting chlorophyll a from photodegradation and oxidation using C-PC aqueous medium.

Rapid recognition of bacteremia in humans using atmospheric pressure chemical ionization mass spectrometry of volatiles emitted by blood cultures

RSC Advances ◽  
2015 ◽  
Vol 5 (18) ◽  
pp. 13952-13957 ◽  
Author(s):  
Konstantin Chingin ◽  
Juchao Liang ◽  
Yaping Hang ◽  
Longhua Hu ◽  
Huanwen Chen
Keyword(s):  
Mass Spectrometry ◽  
Atmospheric Pressure ◽  
Chemical Ionization ◽  
Atmospheric Pressure Chemical Ionization ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass Spectrometry ◽  
Chemical Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical

Human bacteremia is rapidly diagnosed by direct atmospheric pressure chemical ionization mass spectrometry analysis of blood culture volatiles.

scholarly journals Metabolite Profiling of Manilkara zapota L. Leaves by High-Resolution Mass Spectrometry Coupled with ESI and APCI and In Vitro Antioxidant Activity, α-Glucosidase, and Elastase Inhibition Assays

2020 ◽  
Vol 22 (1) ◽  
pp. 132
Author(s):  
Syful Islam ◽  
Md Badrul Alam ◽  
Hyeon-Jin Ann ◽  
Ji-Hyun Park ◽  
Sang-Han Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atmospheric Pressure ◽  
Chemical Ionization ◽  
High Resolution Mass Spectrometry ◽  
Atmospheric Pressure Chemical Ionization ◽  
Ionization Mass ◽  
Manilkara Zapota ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical

High-resolution mass spectrometry equipped with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources was used to enhance the characterization of phytochemicals of ethanol extracts of Manilkara zapota L. leaves (ZLE). Sugar compounds, dicarboxylic acids, compounds of phenolic acids and flavonoids groups, and other phytochemicals were detected from the leaves. Antioxidant activity and inhibition potentiality of ZLE against α-glucosidase enzyme, and elastase enzyme activities were evaluated in in vitro analysis. ZLE significantly inhibited activities of α-glucosidase enzyme at a lower concentration (IC50 2.51 ± 0.15 µg/mL). Glucose uptake in C2C12 cells was significantly enhanced by 42.13 ± 0.15% following the treatment with ZLE at 30 µg/mL. It also exhibited potential antioxidant activities and elastase enzyme inhibition activity (IC50 27.51 ± 1.70 µg/mL). Atmospheric pressure chemical ionization mass spectrometry (APCI–MS) detected more m/z peaks than electrospray ionization mass spectrometry (ESI–MS), and both ionization techniques illustrated the biological activities of the detected compounds more thoroughly compared to single-mode analysis. Our findings suggest that APCI along with ESI is a potential ionization technique for metabolite profiling, and ZLE has the potential in managing diabetes by inhibiting α-glucosidase activity and enhancing glucose uptake.

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