scholarly journals Chemometric Analysis of Extracts and Fractions from Green, Oxidized, and Microbial Fermented Teas and Their Correlation to Potential Antioxidant and Anticancer Effects

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1015 ◽  
Author(s):  
Chan-Su Rha ◽  
Young Sung Jung ◽  
Jung-Dae Lee ◽  
Davin Jang ◽  
Mi-Seon Kim ◽  
...  
Keyword(s):  
Discriminant Analysis ◽  
Phenolic Compounds ◽  
Green Tea ◽  
Antiproliferative Effects ◽  
Multivariate Correlation ◽  
Anticancer Effects ◽  
Antioxidant Capacities ◽  
Hydrophilic Fraction ◽  
Hydrophobic Fraction

Previous reports on phytochemicals in green tea (GT) and processed teas mainly focused on more representative compounds such as catechins. Here, we focus on the insignificantly studied non-catechin components in tea extracts, and explore the multivariate correlation between diverse phenolic compounds in tea and the in vitro antioxidant and anticancer effects. Extracts from GT and four types of processed teas were further divided into hydrophilic and hydrophobic fractions, whose phenolic compositions and antioxidant capacities were quantified using HPLC-MS and three antioxidant assays, respectively. For three types of teas, the anticancer effects of their extracts and fractions were assessed using cancer cell lines. The hydrophobic fractions had lower antioxidant capacities than the corresponding hydrophilic fractions, but exhibited superior antiproliferative effects on cancer cells compared with the whole extract and the hydrophilic fraction. Partial least squares-discriminant analysis revealed a strong correlation between the anticancer effects and the theaflavins and flavonols. Therefore, in addition to catechins, the hydrophobic fraction of tea extracts may have beneficial health effects.

scholarly journals Antioxidative, Anti-Inflammatory, and Anticancer Effects of Purified Flavonol Glycosides and Aglycones in Green Tea

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 278 ◽  
Author(s):  
Chan-Su Rha ◽  
Hyun Woo Jeong ◽  
Saitbyul Park ◽  
Siyoung Lee ◽  
Young Sung Jung ◽  
...  
Keyword(s):  
Cell Line ◽  
Green Tea ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Flavonol Glycosides ◽  
Dependent Manner ◽  
Anticancer Effects ◽  
Anti Inflammatory

(1) Background: Extensive research has focused on flavan-3-ols, but information about the bioactivities of green tea flavonols is limited. (2) Methods: In this study, we investigated the antioxidative, anti-inflammatory, and anticancer effects of flavonol glycosides and aglycones from green tea using in vitro cell models. The fractions rich in flavonol glycoside (FLG) and flavonol aglycone (FLA) were obtained from green tea extract after treatment with tannase and cellulase, respectively. (3) Results: FLG and FLA contained 16 and 13 derivatives, respectively, including apigenin, kaempferol, myricetin, and quercetin, determined by mass spectrometry. FLA exhibited higher radical-scavenging activity than that of FLG. FLG and FLA attenuated the levels of intracellular oxidative stress in neuron-like PC-12 cells. The treatment of RAW 264.7 murine macrophages with FLG and FLA significantly reduced the mRNA expression of inflammation-related genes in a dose-dependent manner. Furthermore, FLG and FLA treatments decreased the viability of the colon adenoma cell line DLD-1 and breast cancer cell line E0771. Moreover, the treatment with FLG or FLA combined with paclitaxel had synergistic anticancer effects on the DLD-1 cell line. (4) Conclusions: Flavonols from green tea exerted beneficial effects on health and may be superior to flavan-3-ols.

In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus different herbs

2008 ◽  
Vol 110 (4) ◽  
pp. 859-864 ◽  
Author(s):  
Tzung-Hsun Tsai ◽  
Tsung-Hsien Tsai ◽  
You-Chia Chien ◽  
Chi-Wei Lee ◽  
Po-Jung Tsai
Keyword(s):  
Comparative Study ◽  
Green Tea ◽  
Antimicrobial Activities ◽  
Antioxidant Capacities

scholarly journals Gold(III) Pyridine-Benzimidazole Complexes as Aquaglyceroporin Inhibitors and Antiproliferative Agents

Inorganics ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 123 ◽  
Author(s):  
Brech Aikman ◽  
Margot Wenzel ◽  
Andreia Mósca ◽  
Andreia de Almeida ◽  
Wim Klooster ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Human Cancer ◽  
Donor Ligands ◽  
Antiproliferative Effects ◽  
Human Cancer Cells ◽  
Human Red Blood Cells ◽  
Anticancer Effects ◽  
Protein Isoform ◽  
The Stability

Gold compounds have been proven to be novel and versatile tools for biological applications, including as anticancer agents. Recently, we explored the potential of Au(III) complexes with bi-dentate N-donor ligands as inhibitors of the membrane water and glycerol channels aquaporins (AQPs), involved in different physiological and pathophysiological pathways. Here, eight new Au(III) complexes featuring a pyridine-benzimidazole scaffold have been synthesized and characterized via different methods. The stability of all the compounds in aqueous solution and their reactivity with glutathione have been investigated by UV–visible spectroscopy. The Au(III) compounds, tested for their AQPs inhibition properties in human Red Blood Cells (hRBC), are potent and selective inhibitors of AQP3. Furthermore, the compounds’ antiproliferative effects have been studied in a small panel of human cancer cells expressing AQP3. The complexes show only very moderate anticancer effects in vitro and are mostly active against the melanoma A375 cells, with marked expression of AQP3 at the level of the nuclear membrane. In general, the AQP3 inhibition properties of these complexes hold promises to develop them as chemical probes to study the function of this protein isoform in biological systems.

scholarly journals Compounds Isolated From the Fruits of Xanthium strumarium, Including a New Neo-Lignan, and Their Anticancer Effects

2020 ◽  
Vol 15 (12) ◽  
pp. 1934578X2098278
Author(s):  
Ye-Liang Wen ◽  
Min-Jing Li ◽  
Zhi-Jian Ye ◽  
Yue-Ming Liang ◽  
Xiao-Qun Wei
Keyword(s):  
Cancer Cells ◽  
Inhibitory Concentration ◽  
Human Cancer ◽  
Xanthium Strumarium ◽  
Spectroscopic Methods ◽  
Addition Compound ◽  
Antiproliferative Effects ◽  
Anticancer Effects ◽  
Hct 116

A new neo-lignan, (7′ S,8 ′R)-4′,5′,9′-trihydroxy-4,6-dimethoxy-5,8′-oxyneolign-7-en-9-al (1), along with 5 known compounds (2-6), were isolated from the fruits of Xanthium strumarium. Their structures were elucidated by extensive spectroscopic methods. All the isolates were evaluated for in vitro cytotoxicities against the human cancer lines HepG2, A549, HCT-116, and SGC-7901. Compounds 1 and 3 showed potent antiproliferative effects against A549 cancer cells with half-maximal inhibitory concentration (IC50) values of 11.2 and 8.3 µM, respectively. In addition, compound 3 exhibited moderate cytotoxicity to SGC-7901 cancer cells, with an IC50 value of 12.9 µM.

scholarly journals Antioxidant activity, total phenolic content and flavonoid concentrations of different plant parts of Teucrium polium L. subsp. polium

2012 ◽  
Vol 81 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Milan S. Stankovic ◽  
Neda Niciforovic ◽  
Vladimir Mihailovic ◽  
Marina Topuzovic ◽  
Slavica Solujic
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Green Tea ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Separate Analysis ◽  
Plant Parts ◽  
Teucrium Polium

Total phenolic content, concentration of flavonoids and in vitro antioxidant activity of twenty different extracts from the whole plant and plant parts (leaves, flowers and stems) of <em>Teucrium polium </em>were determined. The total phenolic contents ranged between 14.57 to 157.84 mg of GaA/g of extract. The concentrations of flavonoids varied from 6.48 to 139.87 mg of Ru/g of extract. Antioxidant activity was determined in vitro using DPPH reagent and expressed as concentration of each extract required to inhibit radical by 50% (<em>IC</em><sub>50</sub>) values that ranged from 26.30 to 2190.75 µg/ml. The methanolic leaves extract contain the greatest concentration of phenolic compounds (157.84 mg of GaA/g) and showed strong antioxidant activity (<em>IC</em><sub>50</sub> = 26.30 µg/ml). Ginkgo and Green tea extracts were analyzed for comparison, and the results indicated that some extracts of <em>T. polium </em>were equal in activity with Ginkgo or Green tea and some appeared to have greater activity. The obtained results suggest strong antioxidant activity and large contribution of separate analysis for the maximum exploitation of active phenolic compounds from <em>T. polium</em>. Based on this information, plant parts of this plant are natural sources of antioxidant substances of high importance.

scholarly journals Biologic Activities of Honeybee Products Obtained From Different Phytogeographical Regions of Turkey

2014 ◽  
Vol 2 (6) ◽  
pp. 273
Author(s):  
Hamide Doğan ◽  
Ethem Akyol ◽  
Hasan Akgül ◽  
Zeliha Selamoğlu Talas
Keyword(s):  
Oxidative Stress ◽  
Phenolic Compounds ◽  
Total Antioxidant Capacity ◽  
Geographical Origin ◽  
Natural Antioxidants ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Antioxidant Capacities ◽  
Total Antioxidant

Honeybee products are rich in phenolic compounds, which effect as natural antioxidants. These compounds may be attached as indicators in studies into the floral and geographical origin of the natural bee products. In this study, we aimed to determine average total antioxidant capacity, average total oxidant capacity and average oxidative stress index of natural bee products obtained from different regions of Turkey. Collected honeybee samples were kept at +4o C until extracted. Natural bee products were extracted with specific methods and antioxidant capacities were defined with in vitro analyses and data were compared. As a result, the highest average total antioxidant capacities were observed in propolis and pollen samples. Total antioxidant capacities of honeybee products collected from various regions demonstrated differences (P

scholarly journals Chemical Characterization of Sambucus nigra L. Flowers Aqueous Extract and Its Biological Implications

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1222
Author(s):  
Pedro Ferreira-Santos ◽  
Helder Badim ◽  
Ângelo C. Salvador ◽  
Armando J. D. Silvestre ◽  
Sónia A. O. Santos ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Life Quality ◽  
Chemical Characterization ◽  
Antiproliferative Effects ◽  
Gram Positive Bacteria ◽  
Flower Extract ◽  
Hydrophilic Fraction ◽  
Different Temperatures ◽  
Hct 116

The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 °C). The extract obtained at 90 °C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was analyzed by GC-MS and HPLC-MS, which allowed the identification of 46 compounds, being quercetin and chlorogenic acid derivatives representative of 86% of the total of phenolic compounds identified in hydrophilic fraction of the aqueous extract. Naringenin (27.2%) was the major compound present in the lipophilic fraction. The antiproliferative effects of the S. nigra extract were evaluated using the colon cancer cell lines RKO, HCT-116, Caco-2 and the extract’s antigenotoxic potential was evaluated by the Comet assay in RKO cells. The RKO cells were the most susceptible to S. nigra flower extract (IC50 = 1250 µg mL−1). Moreover, the extract showed antimicrobial activity against Gram-positive bacteria, particularly Staphylococcus aureus and S. epidermidis. These results show that S. nigra-based extracts can be an important dietary source of bioactive phenolic compounds that contribute to health-span improving life quality, demonstrating their potential as nutraceutical, functional foods and/or cosmetic components for therapeutic purposes.

scholarly journals Chemical Analysis and Cytotoxic and Cytostatic Effects of Twelve Honey Samples Collected from Different Regions in Morocco and Palestine

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Hamada Imtara ◽  
Abdalsalam Kmail ◽  
Soumaya Touzani ◽  
Mira Khader ◽  
Hadeel Hamarshi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Cell Line ◽  
Tannic Acid ◽  
Cancer Cell Line ◽  
Syringic Acid ◽  
Cytostatic Activity ◽  
Strong Negative Correlation ◽  
Anticancer Effects ◽  
Quantitative Hplc Analysis

The aim of this in vitro study is to characterize the phenolic compounds of twelve honey samples collected from different locations in Palestine (H1-6) and Morocco (H7-12) and to evaluate their cytotoxic and cytostatic effects in cells from the human colorectal carcinoma cell line HCT-116 and breast cancer cell line MCF-7. Quantitative HPLC analysis revealed nine phenolic compounds in three Moroccan honey samples, namely, syringic acid, tannic acid, caffeic acid, ferulic acid, coumaric acid, gallic acid, rosmarinic acid, epicatechin, and pyrogallol. Syringic acid, abundant in numerous types of honey with strong antioxidant capacities, was present at values ranging between 0.10 mg/100 g and 1.24 mg/100 g of Daghmos (H11) and Kabbar (H10) samples, respectively. No significant reductions in cell viability were observed in both cell lines treated with the Palestinian samples as measured with MTT assay. Significant cytostatic effects were after treatment of HCT cells with Morar honey H1 with IC50 of 1789 μg/ml. Three Moroccan samples, H7 (Zaâtar), H9 (Bochnikha), and H10 (Kabbar), showed slight, but significant cytostatic effects in HCT cells. A strong correlation was observed between cytostatic activity of MCF cells and antioxidant content (phenols, flavonoids, and flavonol). Furthermore, a strong negative correlation was detected between the cytostatic activity in HCT cells and the contents of syringic acid (r= -0.756) and tannic acid (r= -0.610). These results indicate that the traditionally known anticancer effects of honey might be mediated in part through cytostatic effects.

scholarly journals Comparative effects of extracted polyphenols from black and green tea wastes on in-vitro fermentability of feed ingredients

2021 ◽  
pp. 2005-2022
Author(s):  
Nazak Shokrani Gheshlagh ◽  
◽  
Hamid Paya ◽  
Akbar Taghizadeh ◽  
Hamid Mohammadzadeh ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Green Tea ◽  
Dry Matter ◽  
Black Tea ◽  
Enzymatic Digestion ◽  
Gas Production ◽  
Total Phenolic ◽  
Tea Waste ◽  
Green And Black Tea

The effect of using black and green tea waste extracts on the diet nutritional value was investigated with three methods of in vitro degradability, gas production, and McNiven’s enzymatic digestion in the present experiment. Total phenolic compounds and total tannin for green tea extract were 20.3% and 14.8% of dry matter (DM), respectively, and for black tea were 18.7% and 12.7% of dry matter, respectively. The addition of green and black tea waste extracts reduced both gas production during incubation hours and dry matter degradability (DMD) in the diet (p < 0.05). A significantly reduced ruminal dry matter disappearance (p < 0.05) and a significantly increased post-ruminal dry matter disappearance (p < 0.05) were observed by adding black and green tea waste extracts. According to the results, the dry matter disappearance throughout the gastrointestinal tract was not significantly different between treatments. It can generally be concluded that the addition of phenolic compounds can be used depending on the nutritional strategy, such as reducing ruminal degradability and increasing rumen nutrient escape.

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