scholarly journals Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 733
Author(s):  
Francisco Javier Leyva-Jiménez ◽  
Antonio Jesús Ruiz-Malagón ◽  
José Alberto Molina-Tijeras ◽  
Patricia Diez-Echave ◽  
Teresa Vezza ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Fat Diet ◽  
High Fat ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
Anti Inflammatory ◽  
Tof Ms

Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH• radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.

scholarly journals Column Liquid Chromatography/Electrospray Ionization-Time of Flight-Mass Spectrometry and Ultraperformance Column Liquid Chromatography/Mass Spectrometry Methods for the Determination of Ginkgolides and Bilobalide in the Leaves of Ginkgo biloba and Dietary Supplements

2009 ◽  
Vol 92 (2) ◽  
pp. 645-652 ◽  
Author(s):  
Bharathi Avula ◽  
Yan-Hong Wang ◽  
Troy J Smillie ◽  
Ikhlas A Khan
Keyword(s):  
Mass Spectrometry ◽  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
Electrospray Ionization ◽  
Ginkgo Biloba ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Abstract Column liquid chromatography/electrospray ionization-time of flight-mass spectrometry (LC/ESI-TOF-MS) and ultraperformance column liquid chromatographic/MS (UPLC/MS) methods were developed for the determination of 5 terpenelactones in the leaves of Ginkgo biloba. The separation was performed using a C18 column and a mobile phase composed of water and acetonitrile. The limit of detection (LOD) values for ginkgolide J, ginkgolide C, ginkgolide B, and ginkgolide A were found to be in the range of 110 ng/mL by both methods, and the bilobalide LOD was 200 ng/mL by LC/ESI-TOF and 35 ng/mL by UPLC/MS. Using the optimized method, 4 commercial products were analyzed. Concentrations of total marker compounds in these products were in the 0.0915.4 range, with individual ginkgo terpenelactones being detected at ppb levels. LC/MS with ESI is described for the identification and quantification of ginkgolides and bilobalide in plant samples and dietary supplements that claim to contain G. biloba. These methods involve the use of the M NH4<sup/> and M H<sup/> ions of sesquiterpenoids in the positive ion mode with extractive ion monitoring by LC/ESI-TOF-MS and selected ion monitoring by UPLC/MS. The LC method coupled with MS analysis yielded well-separated peaks which resulted in a clear identification of the compounds.

scholarly journals Population Genotyping of Hepatitis C Virus by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Short DNA Fragments

2005 ◽  
Vol 51 (7) ◽  
pp. 1123-1131 ◽  
Author(s):  
Yoon Jun Kim ◽  
Soo-Ok Kim ◽  
Hyun Jae Chung ◽  
Mi Sun Jee ◽  
Byeong Gwan Kim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pcr Amplification ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Abstract Background: Identifying hepatitis C virus (HCV) genotypes has become increasingly important for determining clinical course and the outcome of antiviral therapy. Here we describe the development of restriction fragment mass polymorphism (RFMP) analysis, a novel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) assay suitable for high-throughput, sensitive, specific genotyping of multiple HCV species. Methods: The assay is based on PCR amplification and mass measurement of oligonucleotides containing genotype-specific motifs in the 5′ untranslated region, into which a type IIS restriction endonuclease recognition was introduced by PCR amplification. Enzymatic cleavage of the products led to excision of multiple oligonucleotide fragments representing variable regions whose masses were determined by MALDI-TOF MS. Results: The RFMP assay identified viral genotypes present at concentrations as low as 0.5% and reliably determined their relative abundance. When sera from 318 patients were analyzed, the RFMP assay exhibited 100% concordance with results obtained by clonal sequencing and identified mixed-genotype infections in 22% of the samples, in addition to several subtype variants. Conclusions: The RFMP assay has practical advantages over existing methods, including better quantitative detection of mixed populations and detection of genotype variants without need for population-based cloning, enabling reliable viral genotyping in laboratories and efficient study of the relationship between viral genotypes and clinical outcome.

Top-down N-terminal sequencing of Immunoglobulin subunits with electrospray ionization time of flight mass spectrometry

2009 ◽  
Vol 384 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Da Ren ◽  
Gary D. Pipes ◽  
David Hambly ◽  
Pavel V. Bondarenko ◽  
Michael J. Treuheit ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Time Of Flight ◽  
Top Down ◽  
Ionization Time ◽  
Flight Mass Spectrometry
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