scholarly journals Carotenoids, Fatty Acids, and Volatile Compounds in Apricot Cultivars from Romania—A Chemometric Approach

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 562
Author(s):  
Adela Pintea ◽  
Francisc Vasile Dulf ◽  
Andrea Bunea ◽  
Sonia Ancuța Socaci ◽  
Elena Andreea Pop ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
High Performance ◽  
Atmospheric Pressure Chemical Ionization ◽  
Principal Component ◽  
Gas Chromatography Mass Spectrometry ◽  
Carotenoid Content ◽  
Array Detector ◽  
Fatty Acids Profile ◽  
Pressure Chemical Ionization

Lipophilic constituents are important for the color and aroma of apricots, but also for their health benefits. In the present study, carotenoids, fatty acids, and volatiles were analyzed in 11 apricot cultivars, from which nine were obtained in Romania. High performance liquid chromatography coupled to a diode array detector with atmospheric pressure chemical ionization and mass spectrometry (HPLC-DAD-APCI-MS methodology applied on unsaponified carotenoid extracts allowed the identification and quantification of 19 compounds. The predominant carotenoids in all cultivars were all-trans-β-carotene and its cis isomers. Lutein was present exclusively in non-esterified form, while β-cryptoxanthin was predominantly esterified, mainly with oleic, palmitic, lauric, and stearic acid. Moreover, β-cryptoxanthin linoleate, linolenate, and stearate were detected for the first time in Harogem cultivar. Variation in carotenoid content and composition was observed, with the highest carotenoid content being recorded in Tudor, Harogem, and Mamaia cultivars. The predominant fatty acids determined by gas chromatography–mass spectrometry (GC-MS) were linoleic (up to 47%), palmitic (up to 32.7%), and linolenic (up to 17.16%), with small variations among cultivars. In-tube extraction technique (ITEX)/GC-MS was applied for profiling the volatiles in apricot fruits and 120 compounds were identified, with terpenoids and esters as the most abundant classes. Principal component analysis (PCA) revealed that the carotenoids and the fatty acids profile can be used for variety authentication and discrimination in apricots.

scholarly journals Quali-Quantitative Profile of Native Carotenoids in Kumquat from Brazil by HPLC-DAD-APCI/MS

Foods ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 166 ◽  
Author(s):  
Helena Maria Pinheiro-Sant’Ana ◽  
Pamella Cristine Anunciação ◽  
Clarice Silva e Souza ◽  
Galdino Xavier de Paula Filho ◽  
Andrea Salvo ◽  
...  
Keyword(s):  
High Performance ◽  
Atmospheric Pressure Chemical Ionization ◽  
Carotenoid Content ◽  
Free Form ◽  
Array Detector ◽  
Ionization Mass ◽  
Total Carotenoids ◽  
Fresh Matter ◽  
Total Carotenoid Content ◽  
Pressure Chemical Ionization

In this study the native carotenoids composition in kumquat (Fortunella margarita) (peel + pulp) from Brazil was determined for the first time by a HPLC-DAD-APCI/MS (high performance liquid chromatography-diode array detector-atmospheric pressure chemical ionization/mass spectrometry), methodology. Eleven carotenoids were successfully identified and quantified in kumquat: four carotenoids in the free form and seven carotenoids in the esterified form. β-citraurin-laurate was the carotenoid found in the highest content (607.33 µg/100 g fresh matter), followed by β-cryptoxanthin-laurate (552.59 µg/100 g). The different esterified forms of β-citraurin and β-cryptoxanthin represented 84.34% of the carotenoids found, which demonstrates the importance of esterification in natural fruits. β-carotene and free xanthophylls (β-cryptoxanthin, lutein and zeaxanthin) represented 5.50% and 14.96%, respectively, of total carotenoids in kumquat. The total carotenoid content of kumquat from Brazil was very high (2185.16 µg/100 g), suggesting that this fruit could contribute significantly to the intake of important bioactive compounds by the population.

scholarly journals Determination of stigmasterol hydroperoxides using high-performance liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S144-S146 ◽  
Author(s):  
S. Kemmo ◽  
V. Ollilainen ◽  
Lampi A-M ◽  
V. Piironen
Keyword(s):  
Mass Spectrometry ◽  
Hydrogen Peroxide ◽  
High Performance Liquid Chromatography ◽  
Atmospheric Pressure ◽  
High Performance ◽  
Chemical Ionization ◽  
Atmospheric Pressure Chemical Ionization ◽  
Liquid Chromatography Mass Spectrometry ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical

A new specific method using high-performance liquid chromatography-mass spectrometry (HPLC-MS) for the detection of stigmasterol hydroperoxides was developed. Hydroperoxides of stigmasterol were obtained by photo-oxidation (90 min) in the presence of methylene blue as a sensitizer. The separation was performed using normal-phase chromatographic conditions. The MS detection was carried out with an ion-trap mass spectrometer using atmospheric pressure chemical ionization (APCI) and positive ion mode. Stigmasterol hydroperoxides were seen to produce no protonated molecular ions [M + H]<sup>+</sup> but instead fragments representing loss of one or two water molecules [M – H<sub>2</sub>O + H]<sup>+</sup>, [M – 2H<sub>2</sub>O + H]<sup>+</sup>, loss of hydrogen peroxide [M – H<sub>2</sub>O<sub>2</sub> + H]<sup>+</sup> or loss of hydrogen peroxide and water [M – H<sub>2</sub>O<sub>2</sub> – H<sub>2</sub>O + H]<sup>+</sup>. The results showed that positional isomers of hydroperoxides had different fragmentation patterns and relative ion abundances. On the other hand anomeric isomers had more similar fragmentation. As a conclusion the method developed showed to be a useful tool for investigation the oxidation mechanism of sterols.

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