scholarly journals Rosemary Diterpenes and Flavanone Aglycones Provide Improved Genoprotection against UV-Induced DNA Damage in a Human Skin Cell Model

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 255 ◽  
Author(s):  
Noelia Sánchez-Marzo ◽  
Almudena Pérez-Sánchez ◽  
Enrique Barrajón-Catalán ◽  
Julián Castillo ◽  
María Herranz-López ◽  
...  
Keyword(s):  
Dna Damage ◽  
Metal Ion ◽  
Cell Model ◽  
Radical Scavenging ◽  
Human Keratinocytes ◽  
Ros Generation ◽  
Uvb Radiation ◽  
Mitochondrial Depolarization ◽  
Skin Cell ◽  
Intracellular Ros

Overexposure to solar ultraviolet (UV) radiation is the major cause of a variety of cutaneous disorders, including sunburn, photoaging, and skin cancers. UVB radiation (290–320 nm) causes multiple forms of DNA damage, p53 induction, protein and lipid oxidation, and the generation of harmful reactive oxygen species (ROS). In recent years, botanicals containing polyphenols with antioxidant and anti-inflammatory properties as skin photoprotective agents have emerged. This study evaluated the protective effects of two formulations against UVB-induced damage in a skin cell model. One of the formulations (F2) contained a combination of citrus and olive extracts and the other one (F1) also contained a rosemary extract. The antioxidant capacity of both formulations was estimated by different in vitro methods, and the cell viability, intracellular ROS generation, mitochondrial depolarization, and DNA damage were studied in UVB-irradiated human keratinocytes. Both formulations exerted photoprotective effects on skin cells and decreased mitochondrial depolarization and DNA damage. F1 which contained iridoids, rosemary diterpenes, glycosides and aglycones of citrus flavanones, and monohydroxylated flavones exhibited higher cellular photoprotective effects and mitochondrial membrane potential restoration, as well as an enhanced capacity to decrease DNA double strand breaks and the DNA damage response. In contrast, F2, which contained mostly iridoids, citrus flavanone aglycones, and mono- and dihydroxylated flavones, exhibited a higher capacity to decrease intracellular ROS generation and radical scavenging capacity related to metal ion chelation. Both formulations showed a similar capability to decrease the number of apoptotic cells upon UVB radiation. Based on our results and those of others, we postulate that the stronger capacity of F1 to protect against UVB-induced DNA damage in human keratinocytes is related to the presence of rosemary diterpenes and citrus flavanone aglycones. Nevertheless, the presence of the dihydroxylated flavones in F2 may contribute to inhibiting the generation of metal-related free radicals. To confirm the efficacy of these formulations as potential candidates for oral/topical photoprotection, human trials are required to circumvent the limitations of the cellular model.

scholarly journals Carbon Monoxide Partially Mediates Protective Effect of Resveratrol Against UVB-Induced Oxidative Stress in Human Keratinocytes

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 432 ◽  
Author(s):  
Janice N. Averilla ◽  
Jisun Oh ◽  
Jong-Sang Kim
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Radical Scavenging ◽  
Protoporphyrin Ix ◽  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Related Factor ◽  
Uvb Radiation ◽  
Intracellular Ros ◽  
Tin Protoporphyrin

Based on the antioxidative effect of resveratrol (RES) in mitigating reactive oxygen species (ROS) production through the induction of nuclear factor-erythroid 2-related factor-2 (Nrf2)/heme oxigenase-1 (HO-1) signaling pathway, we investigated whether the protective activity of RES against ROS-mediated cytotoxicity is mediated by intracellular carbon monoxide (CO), a product of HO-1 activity, in ultraviolet B (UVB)-irradiated human keratinocyte HaCaT cells. The cells were exposed to UVB radiation following treatment with RES and/or CO-releasing molecule-2 (CORM-2). RES and/or CORM-2 upregulated HO-1 protein expression, accompanied by a gradual reduction of UVB-induced intracellular ROS levels. CORM-2 reduced intracellular ROS in the presence of tin protoporphyrin IX, an HO-1 inhibitor, indicating that the cytoprotection observed was mediated by intracellular CO and not by HO-1 itself. Moreover, CORM-2 decreased RES-stimulated mitochondrial quantity and respiration and increased the cytosolic protein expressions of radical-scavenging superoxide dismutases, SOD1 and SOD2. Taken together, our observations suggest that RES and intracellular CO act independently, at least partly, in attenuating cellular oxidative stress by promoting antioxidant enzyme expressions and inhibiting mitochondrial respiration in UVB-exposed keratinocytes.

145 STAGE-SPECIFIC EFFECT OF OXIDATIVE STRESS ON DEVELOPMENTAL COMPETENCE, ROS GENERATION AND DNA DAMAGE OF PORCINE PARTHENOGENETIC EMBRYOS

2006 ◽  
Vol 18 (2) ◽  
pp. 180
Author(s):  
M. Takahashi ◽  
M. Sakatani ◽  
S. Kobayashi ◽  
S. Kobayashi ◽  
H. Nagashima
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Fluorescent Dyes ◽  
Specific Effect ◽  
Ros Generation ◽  
Blastocyst Formation ◽  
Comet Tail ◽  
Intracellular Ros ◽  
O2 Concentration ◽  
High O2

We investigated the effect of oxidative stress on stage specific developmental ability, reactive oxygen species (ROS) generation and DNA damage of parthenogenetically activated porcine embryos. Cumulus-oocyte complexes (COCs) were aspirated from follicles on the surface of ovaries. The COCs were matured in NCSU-23 containing 10% (vol/vol) porcine follicular fluid and 10 IU/mL hCG during the first 22 h followed by an extra 22 h of culture in the hormone free NCSU-23. After 44 h of maturation, oocytes were denuded of cumulus cells and used for activation. Oocytes were activated by a 100-�sec pulse of 1.5 kV/cm DC with 1-mm electrodes in 0.3 m mannitol, 0.1 mm MgSO4, and 0.05 mm CaCl2. Activated oocytes were then cultured for 5 h in NCSU-23 containing 5 mg/mL BSA, 10 �g/mL EGF and 7.5 �g/mL cytochalasin B. Embryos were then cultured for 6 days in PZM-5. In Experiment 1, after parthenogenetic activation, embryos were cultured at 38.5�C under 5% O2, 5% CO2 and 90% N2 (defined as 5% O2) or 5% CO2 in air (20% O2). The oxygen concentration for embryo culture was changed from 5% to 20% on day 1, 2, 3, 4, and 5 post-activation, respectively. Embryos were also cultured throughout 6 days in 5 and 20% O2. About 100 embryos were used in each experiment. The number of embryos cleaved and developed to blastocyst stage was observed on day 2 and 6, respectively. In Experiment 2, 10 to 20 embryos cultured in 5 and 20% O2 were collected on Days 2, 4, and 6 for the detection of ROS, intracellular glutathione (GSH) levels and DNA damage. Intracellular ROS and GSH levels, were measured with fluorescent dyes (22,72-dichlorodihydrofluorescein diacetate for ROS and Cell Tracker" Blue for GSH). DNA damage of individual embryos was detected with a comet assay. DNA damage was quantified by measuring the length of the streak of DNA comet tail between the edge of the zona pellucida and the end of the visible comet tail by image analysis software. The rate of migrated DNA area per total DNA was also quantified. In Exp. 1, the rate of blastocyst formation was significantly decreased (P < 0.001) when embryos were cultured for 6 days under 20% O2 (17.8 � 4%) than 5% O2 (38.5 � 5%). The rates of blastocyst formation were significantly decreased (P < 0.05) when O2 concentration was changed from 5 to 20% before Day 3. After Day 4, high O2 concentration did not affect the development. In Exp. 2, relative ROS levels were significantly higher (P < 0.05) on Day 2 (1.5 � 0.03) and Day 4 (1.4 � 0.06) in embryos cultured under 20% O2 than in those cultured under 5% O2 (1.0). No difference was observed in GSH level. DNA damage was significantly increased (P < 0.05) in Day 2 embryos cultured under 20% O2 (161 � 54 �m) than 5% O2 (65 � 8.8 �m). These results indicate that the oxidative stress to embryo development by high O2 concentration is stage specific, that embryos are more sensitive in early stages, and that the oxidative stress has correlation with the increase of intracellular ROS and DNA damage.

Dose- and duration-dependent cytotoxicity and genotoxicity in human hepato carcinoma cells due to CdTe QDs exposure

2019 ◽  
Vol 38 (8) ◽  
pp. 914-926 ◽  
Author(s):  
KM Katubi ◽  
FM Alzahrani ◽  
D Ali ◽  
S Alarifi
Keyword(s):  
Dna Damage ◽  
Apoptotic Cell ◽  
Average Particle Size ◽  
Carcinoma Cells ◽  
Ros Generation ◽  
Dna Integrity ◽  
Average Particle ◽  
Cdte Qds ◽  
Intracellular Ros ◽  
Mechanism Of Toxicity

Nanotechnology has achieved more commercial attention over recent years, and its application has increased concerns about its discharge in the environment. In this study, we have chosen human hepatic carcinoma (HuH-7) cells because liver tissue has played an important role in human metabolism. Therefore, the objective of this study was to determine DNA damaging and apoptotic potential of cadmium telluride quantum dots (CdTe QDs; average particle size (APS) 10 nm, 1–25 µg/ml) on HuH-7 cells and the basic molecular mechanism of its cellular toxicity. Cytotoxicity of different concentrations of CdTe QDs on HuH-7 cells was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and lactate dehydrogenase (LDH) tests. Moreover, reactive oxygen species (ROS) generation, mitochondrial membrane potential, DNA damage, and Hoechst 33342 fluorescent staining morphological analysis of necrotic/apoptotic cells were detected; cellular impairment in mitochondria and DNA was confirmed by JC-1 and comet assay, respectively. A dose- and time-dependent cytotoxicity effect of CdTe QDs exposure was observed HuH-7 cells; the significant ( p < 0.05) cytotoxicity was found at 25 μg/ml of CdTe QDs exposure. The percentage of cytotoxicity of CdTe QDs (25 μg/ml) in HuH-7 cells reached 62% in 48 h. CdTe QDs elicited intracellular ROS generation and mitochondrial depolarization, and DNA integrity cells collectively advocated the apoptotic cell death at higher concentration. DNA damage was observed in cells due to CdTe QDs exposure, which was mediated by oxidative stress. This study exploring the effects of CdTe QDs in HuH-7 cells has provided valuable insights into the mechanism of toxicity induced by CdTe QDs.

scholarly journals A New Natural Antioxidant Biomaterial from Cinnamomum osmophloeum Kanehira Leaves Represses Melanogenesis and Protects against DNA Damage

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 474 ◽  
Author(s):  
Yung-Shu Ho ◽  
Jane-Yii Wu ◽  
Chi-Yue Chang
Keyword(s):  
Dna Damage ◽  
Metal Ion ◽  
Steam Distillation ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Chemical Compositions ◽  
Melanin Synthesis

Cinnamomoum osmophloeum Kanehira (COK) is an indigenous tree species in Taiwan. Chemical compositions, antioxidant activity, mushroom tyrosinase inhibition, melanin synthesis repression, and protection against DNA damage of hydrosol from the COK leaves by steam distillation were examined. We performed 1,1-diphenyl-2-picrylhydrazyl radical scavenging, metal ion chelating, reducing power, and Trolox equivalent antioxidant capacity (TEAC) assays and determined the correlations between total phenolic contents and antioxidant activities. The findings showed that the anti-oxidative properties of COK hydrosol are closely correlated with their phenol contents. Additionally, the major constituents of hydrosol, i.e., cinnamaldehyde and benzaldehyde, had dose-dependent anti-tyrosinase effects against both monophenolase and diphenolase activities. GC-MS analysis revealed that the major bioactive components of hydrosol were trans-cinnamaldehyde (87.7%), benzaldehyde (7.0%), and cinnamyl acetate (5.3%). Moreover, we found that the hydrosol with the presence of benzaldehyde is more potent than pure cinnamaldehyde, and enhances the tyrosinase inhibitory activity of hydrosol. In kinetic analyses, Lineweaver–Burk plots and replots showed that COK hydrosol is a mixed-type inhibitor. Additionally, we found that very low doses of COK hydrosol repressed α-melanocyte-stimulating hormone-induced synthesis of microphthalmia-associated transcription factor, leading to decreased melanin synthesis in B16-F10 melanoma cells. These results demonstrated that production of hydrosol from COK leaves using steam distillation may provide a safe and efficacious source of skin-whitening agents for cosmetic and pharmaceutical applications, with antioxidant, anti-tyrosinase, anti-melanogenesis, and DNA protective activities.

scholarly journals Antioxidant and Photoprotective Activity of Apigenin and its Potassium Salt Derivative in Human Keratinocytes and Absorption in Caco-2 Cell Monolayers

2019 ◽  
Vol 20 (9) ◽  
pp. 2148 ◽  
Author(s):  
Sánchez-Marzo ◽  
Pérez-Sánchez ◽  
Ruiz-Torres ◽  
Martínez-Tébar ◽  
Castillo ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Intestinal Absorption ◽  
Potassium Salt ◽  
Water Solubility ◽  
Cell Model ◽  
Human Keratinocytes ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Antioxidant Power

Ultraviolet (UV) radiation, especially types A (UVA) and B (UVB), is one of the main causes of skin disorders, including photoaging and skin cancer. Ultraviolent radiation causes oxidative stress, inflammation, p53 induction, DNA damage, mutagenesis, and oxidation of various molecules such as lipids and proteins. In recent decades, the use of polyphenols as molecules with an antioxidant and anti-inflammatory capacity has increased. However, some of these compounds are poorly soluble, and information regarding their absorption and bioavailability is scarce. The main objective of this study was to compare the intestinal absorption and biological activity of apigenin and its more soluble potassium salt (apigenin-K) in terms of antioxidant and photoprotective capacity. Photoprotective effects against UVA and UVB radiation were studied in human keratinocytes, and antioxidant capacity was determined by different methods, including trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Finally, the intestinal absorption of both apigenins was determined using an in vitro Caco-2 cell model. Apigenin showed a slightly higher antioxidant capacity in antioxidant activity assays when compared with apigenin-K. However, no significant differences were obtained for their photoprotective capacities against UVA or UVB. Results indicated that both apigenins protected cell viability in approximately 50% at 5 J/m2 of UVA and 90% at 500 J/m2 of UVB radiation. Regarding intestinal absorption, both apigenins showed similar apparent permeabilities (Papp), 1.81 × 10−5 cm/s and 1.78 × 10−5 cm/s, respectively. Taken together, these results suggest that both apigenins may be interesting candidates for the development of oral (nutraceutical) and topical photoprotective ingredients against UVA and UVB-induced skin damage, but the increased water solubility of apigenin-K makes it the best candidate for further development.

scholarly journals Amorphous nanosilica induce endocytosis-dependent ROS generation and DNA damage in human keratinocytes

2011 ◽  
Vol 8 (1) ◽  
pp. 1 ◽  
Author(s):  
Hiromi Nabeshi ◽  
Tomoaki Yoshikawa ◽  
Keigo Matsuyama ◽  
Yasutaro Nakazato ◽  
Saeko Tochigi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Keratinocytes ◽  
Ros Generation
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