scholarly journals Use of Sieving as a Valuable Technology to Produce Enriched Buckwheat Flours: A Preliminary Study

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 583 ◽  
Author(s):  
Beatriz Martín-García ◽  
Federica Pasini ◽  
Vito Verardo ◽  
Ana María Gómez-Caravaca ◽  
Emanuele Marconi ◽  
...  
Keyword(s):  
Particle Size ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Starch Content ◽  
Salt Content ◽  
Total Phenolic ◽  
Physical Separation ◽  
Preliminary Study ◽  
Total Starch

Fractionation processes based on physical separation are a good strategy to produce enriched cereal flours. Therefore, the aim of this work is to evaluate the suitability of sieving of buckwheat flours to produce protein and phenolic (especially rutin) enriched fractions. Because of that, dehulled whole buckwheat flour (GSTQ) was sieved obtaining fractions with a particle size of 215 µm, 160 µm, 85 µm, and 45 µm (GS215, GS160, GS85, and GS45). For that purpose, the determination of protein, ash, and total starch content and free and bound phenolic compounds was carried out. The highest content of total phenolic compounds was obtained in GS215 (3118.84 mg kg−1 d.w.), followed by GS160 (2499.11 mg kg−1 d.w.), GS85 (989.46 mg kg−1 d.w.), GSTQ (983.15 mg kg−1 d.w.), and GS45 (481.31 mg kg−1 d.w.). Therefore, the phenolic content decreased with the particle size decrease from 215 µm to 45 µm. Besides, there were no significant differences between the total phenolic content in GS85 and GSTQ. The fraction with 215 µm reported the highest protein and mineral salt content and presented rutin amounts four times higher than GSTQ.

scholarly journals Antioxidant Activity and Phenolic Content of Campomanesia Phaea Extracts Obtained by Pressurized Liquid Extraction

2019 ◽  
Vol 11 (1) ◽  
pp. 56-65
Author(s):  
Daniele Martins Firmiano ◽  
Gabriela Marques Bittencourt ◽  
Alessandra Lopes de Oliveira
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Fixed Bed ◽  
Liquid Extraction ◽  
Pressurized Liquid Extraction ◽  
Total Phenolic ◽  
High Antioxidant Activity ◽  
The Mean

Objective: This research used the Pressurized Liquid Extraction process (PLE) to obtain cambuci extracts rich in phenolic compounds and, consequently, with high antioxidant activity. Methods: We determined the centesimal composition and the mean particle size of the dried and crushed sample for characterization of the fixed bed extractor. Extractions with pressurized ethanol were performed following a Central Composite Design (CCD) with temperature (60 °C at the center point with ± 10 °C amplitude) and the contact time between the solvent and the matrix (6 min at the center point with ± 2 min amplitude) as independent variables in the process. The Total Phenolic Content (TPC) of compounds and antioxidant activity were determined. Results: The mean particle size of the cambuci in the PLE was 263.4 μm with apparent and real densities of 32.42 g/cm3 and 1.62 g/cm3, respectively, resulting in a fixed bed porosity of 0.43. The centesimal composition of the comminuted and dried sample was 12.5 ± 0.1% moisture, 3.2 ± 0.2% ash, 3.35 ± 0.07% crude protein, 10.32 ± 0.08% crude fiber and 0.35 ± 0.04% ethereal extract. The highest extraction yield (41.7%) was obtained by using the binomial 70 ºC with 8 min of solvent contact in three batches. Extracts with the highest TPC (6501.10 mg AGE/100 g sample) were obtained by the binomial 74 °C with 6 min of contact; the binomial 50 °C with 4 min of contact gave rise to the extract with the highest antioxidant activity. The CCD showed high yield extracts with high antioxidant activity and a high total phenolic compound content. Conclusion: With the use of the proposed experimental design, it was possible to optimize the extraction of total phenolic compounds from the sample.

Determination of optimal extraction conditions for phenolic compounds from Pistacia atlantica leaves using the response surface methodology

2016 ◽  
Vol 8 (31) ◽  
pp. 6107-6114 ◽  
Author(s):  
Ziyad Ben Ahmed ◽  
Mohamed Yousfi ◽  
Johan Viaene ◽  
Bieke Dejaegher ◽  
Kristiaan Demeyer ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Box Behnken Design ◽  
Pistacia Atlantica

Response surface methodology in combination with a Box–Behnken design was performed to optimize the extraction conditions, resulting in a maximum total phenolic content yield from Pistacia atlantica leaves.

scholarly journals Determination of Total Phenolic Levels in Ethanol Extract of Moringa (Moringa oleifera L.) Leaves based on Differences in Growing Sites

2021 ◽  
pp. 403-411
Author(s):  
Any Guntarti ◽  
Nining Sugihartini ◽  
Siti Athiyah Umaiyah ◽  
Nina Salamah
Keyword(s):  
Phenolic Compounds ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Moringa Oleifera ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Nutritional Content ◽  
Method Analysis

Moringa oleifera L. have good nutritional content including phenolic compounds which can be used as antioxidants and can grow in lowlands and highlands. The purpose of this study was to determine the total phenolic content of the ethanol extract of M. oleifera leaves with variations in the area of ​​collection. The 50% ethanol extract was obtained from the simplicia of M. oleifera leaves by using the maceration method. Analysis of total phenolic content in the extract was carried out using a spectrophotometer instrument with the addition of reagent Folin-Ciocalteu and gallic acid as standard. The results of total phenolic content in Sleman, Wonosari, and Wonosobo areas were (127.87 ± 2.71) mg GAE / g extract, (99.40 ± 2.68) mg GAE / g extract, and (142 , 92 ± 1.81) mg GAE / g extract. The highest phenolic content in the ethanol extract of moringa leaves was found in Wonosobo areas.

scholarly journals Nutritional composition, total phenolic compounds and antioxidant activity of quinoa (Chenopodium quinoa Willd.) of different colours

2018 ◽  
Vol 17 (1) ◽  
pp. 74-85 ◽  
Author(s):  
Yemina Karen Diaz-Valencia ◽  
Juan José Alca ◽  
Maria Antonia Calori-Domingues ◽  
Sonia Jackeline Zanabria-Galvez ◽  
Sandra Helena Da Cruz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Dietary Fibre ◽  
Phenolic Content ◽  
Starch Content ◽  
Chenopodium Quinoa ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Dpph Method

Abstract Quinoa (Chenopodium quinoa Willd.) has been nutritionally highlighted when compared to other grains. In recent years the research on this pseudocereal has increased. In this work, six quinoa samples were studied: three from Peru, one from Brazil and two commercial samples. The samples were physically and physicochemically characterized, including macro- and micronutrient analysis, phenolic compounds content and antioxidant activity. Black, red and white samples showed as main difference the size, weight, ashes and dietary fibre content. Black samples were the smallest and lightest and had the lowest starch content but presented the highest levels of ashes and dietary fibre. The protein content (16.9 %) in the white Brazilian variety was higher than the others. Red and black samples had the highest levels of most minerals analysed. The antioxidant capacity measured by the DPPH method was higher for black and red samples in comparison with the white ones. However, the white Brazilian variety showed a significantly higher antioxidant capacity measured by the ABTS assay. With regard to the phenolic content, a difference was found between the samples which ranged from 55.5 to 95.5 g GAE 100 g−1. The colour of the grain was found as not related to a higher content of phenolic compounds. Because their compositions are generally similar to light-coloured grains, and in some parameters such as dietary fibre and content of some micronutrients are superior, the grains of dark-coloured quinoa varieties (RPP, BCP) would have to be explored to develop foods that take advantage of this colour diversity.

scholarly journals Determination of Antioxidant Activity of Selected Vegetables Grown in Iran under Reversed Phase Conditions with UPLC

2015 ◽  
Vol 9 (5) ◽  
pp. 122-125
Author(s):  
Nahid Rastakhiz ◽  
Fereshteh Khosravi ◽  
Seyyed Sina Seyyed Jafari Olia ◽  
Behzad Iranmanesh
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Reversed Phase ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Reverse Phase

This study describes the determination of some phenolic compounds in four different vegetables of Kerman in Iran including Carrots, Celery, Lettuce and red cabbage .The  phenolic compounds analyzed were (Ascorbate, Ferulic acid, Naringin) using reverse phase high performance liquid chromatography (RPUPLC). The results of analysis showed in vegetables ranged between 90 and 1080mg /kg. A high and significant correlation between antioxidant activity and total phenolic content was determined in vegetables (r2 = 0.9461 P < 0.06). However, flavonoid content was not significantly correlated with antioxidant activity in vegetables. It was observed that total phenolic content is the major contributor to the antioxidant activity of vegetables.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12712

scholarly journals Content of Phenolic Compounds and Organic Acids in the Flowers of Selected Tulipa gesneriana Cultivars

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5627
Author(s):  
Agnieszka Krzymińska ◽  
Monika Gąsecka ◽  
Zuzanna Magdziak
Keyword(s):  
Phenolic Compounds ◽  
Organic Acids ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
Tulipa Gesneriana ◽  
The Mean

The study focused on the determination of phenolic acids, flavonoids and organic acids in five tulip cultivars ‘Barcelona’, ‘Columbus’, ‘Strong Gold’, ‘Super Parrot’ and ‘Tropicana’. The cultivars grown in field and in a greenhouse were exposed after cutting to different times of storage (0, 3 and 6 days). The phenolic profile contained 4-hydroxybenzoic, 2,5-dihydroxybenzoic, gallic, vanillic, syringic, salicylic, protocatechuic, trans-cinnamic, p-coumaric, caffeic, ferulic, chlorogenic and sinapic acids, as well as quercetin, rutin, luteonin, catechin and vitexin. The mean phenolic acid content was in the following order: ‘Columbus’ and ‘Tropicana’ > ’Barcelona’ > ’Strong Gold’ > ’Super Parrot’, while the levels of flavonoids were as follows: ‘Strong Gold’ > ’Barcelona’ > ’Tropicana’ > ’Columbus’ > ’Super Parrot’. The highest content of phenolic acids was confirmed for Columbus and Tropicana, while the lowest was for Super Parrot. However total phenolic content was very similar, observed between the place of cultivation, time of storage and cultivars. Malonic, succinic, acetic and citric acids were the major organic acid components in tulip petals. More organic acids (except malonic) were accumulated in tulip petals from fields than those from the greenhouse, while changes during storage were strictly correlated with cultivars.

scholarly journals Determination of γ-oryzanol, Phenolic total content, and (2,2-difenil-1-picrylhydrazyl) Radical Scavenging Activity in Different Varieties of Rice (Oryza sativa) in Yogyakarta, Indonesia

2018 ◽  
Vol 23 (2) ◽  
pp. 113
Author(s):  
Erna Prawita Setyowati ◽  
Andayana Puspitasari Gani
Keyword(s):  
Oryza Sativa ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Content ◽  
Total Phenolic ◽  
Antioxidants Activity ◽  
Natural Beauty

In Indonesia, beras (Oryza sativa) has become the main ingredient in natural beauty products such as masks, scrubs, and cold powder. γ-oryzanol and phenolic compounds are the major components in beras that have many benefits, one of which as antioxidants. This study aims to determine the content of γ-oryzanol and total phenolic compounds in different beras cultivars in the area of Yogyakarta. The leveling was done by a TLC-densitometry method and Folin Ciocalteu. The results showed that the largest γ-oryzanol content found in red beras was 0.0843% by weight of the sample. The highest total phenolic content was found in red beras as much as 1.92% by weight of the sample. The antioxidants activity IC50 Red Beras is 0.591.

scholarly journals Correlation of Phenolic Content of Multiflora Honey from Malang and Alastuwo to Activity Antioxidant Using DPPH (2.2-Diphenyl-1-Pickrylhydrazyl)

2021 ◽  
Vol 4 (1) ◽  
pp. 1-11
Author(s):  
Ahwan Abdul
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Activity Test ◽  
Dpph Method ◽  
Color Reagent ◽  
Qualitative Test

Honey contains various compounds that function as antioxidants, one of which is phenolic compounds. The purpose of this study was to determine the correlation between the phenolic content of multiflora honey from Malang and Alastuwo on antioxidant activity using the DPPH method (2,2-Diphenyl-1-picrylhydrazyl). Qualitative test of phenolic compounds with FeCl3 color reagent, determination of total phenolic content by UV-Vis spectrophotometry using Folin-Ciocalteu method and antioxidant activity test using DPPH method (2,2-Diphenyl-1-picrylhydrazyl). The results showed that qualitatively Malang and Alastuwo honey contained phenolic compounds. The total phenolic content of Alastuwo honey was 0.0278 ± 0.0010 mg GAE/g and Malang honey was 0.0301 ± 0.0004 mg GAE/g. The results of the antioxidant activity test showed that Alastuwo honey had weak antioxidant activity (IC50 of 393.37 ± 10.28 ppm), Malang honey had moderate antioxidant activity (IC50 of 217.20 ± 6.61 ppm) and vitamin C had strong antioxidant activity. (IC50 is 2.22 ± 0.19 ppm). The total phenolic content of Alastuwo and Malang honey correlates with its antioxidant activity.

scholarly journals Grape Leaves as a Natural Antioxidant Source: Determination of Total Phenolic Compound Changes

2019 ◽  
Vol 7 (10) ◽  
pp. 1603 ◽  
Author(s):  
Zehra Babalık ◽  
Nilgün Göktürk Baydar
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Natural Antioxidants ◽  
Food Supplement ◽  
Total Phenolic ◽  
Source Determination ◽  
Health Promoting ◽  
Grapevine Leaves

In this study, total phenolic compounds which possess health-promoting effects and provide basic nutrition were aimed to determine the alterations in different grapevine leaves. Total phenolic content was determined using Folin-Ciocalteu colorimetric method by using a spectrophotometer. At the result of the study, it was determined that contents of total phenolic in leaves collected from different grape cultivars were varied to cultivars and grape leaves had high phenolic compounds as berries. Therefore the grape leaves especially wine grapes such as Narince, Boğazkere ve Kalecik Karası may be used as an easily accessible source of natural antioxidants and food supplement at pharmaceutical and food industry.

scholarly journals A Greener and Faster Approach for Determination of Phenolic Compounds by Smartphone-Based Colorimetry

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 20
Author(s):  
Luís Claudio Martins ◽  
Anna Flavia de Souza Silva ◽  
Liz Mary Bueno de Moraes ◽  
Isabela Camargo Gonçalves ◽  
Bianca Bacellar Rodrigues de Godoy ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Dry Weight ◽  
Total Phenolic ◽  
Photometric Detection ◽  
Assisted Extraction ◽  
Fold Reduction ◽  
Malpighia Emarginata

This work aims the development of an analytical alternative to determine phenolic compounds in fruits exploiting a greener microwave-assisted extraction and photometric detection by smartphone-based colorimetry. Acerola cherry (Malpighia emarginata) was selected as a model and the extraction was optimized by the Doehlert design, taking into account temperature, solvent composition, and extraction time. The Folin–Ciocalteau method was used to determine total phenolic content in the extracts, with a 5-fold reduction in reagent amount and waste generation. The optimal extraction was achieved with 17% v/v ethanol, at 38 °C, for 50 min. The total phenolic content determined by the proposed procedure (146 ± 4 mg GAE g−1 dry weight) agreed with the reference procedure (145.0 ± 0.7 mg GAE g−1 dry weight) at the 95% of confidence level. Due to these characteristics, the proposed approach is an alternative for screening fruits in research with bioactive compounds.

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