scholarly journals Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 251 ◽  
Author(s):  
Wen-Wan Chao ◽  
Shu-Ju Chen ◽  
Hui-Chen Peng ◽  
Jiunn-Wang Liao ◽  
Su-Tze Chou
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Gallic Acid ◽  
Alternative Therapy ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Sprague Dawley ◽  
Complementary And Alternative Therapy ◽  
Traditional Chinese Herbal Medicine ◽  
Droplet Infiltration

(1) Background: Graptopetalum paraguayense E. Walther is a traditional Chinese herbal medicine. In our previous study, 50% ethanolic G. paraguayense extracts (GE50) demonstrated good antioxidant activity. (2) Methods: To investigate the hepatoprotective effects of GE50 on ethanol and carbon tetrachloride (CCl4) co-induced hepatic damage in rats, Sprague–Dawley rats were randomly divided into five groups (Control group; GE50 group, 0.25 g/100 g BW; EC group: Ethanol + CCl4, 1.25 mL 50% ethanol and 0.1 mL 20% CCl4/100 g BW; EC + GE50 group: Ethanol + CCl4 + GE50; EC + silymarin group: ethanol + CCl4 + silymarin, 20 mg/100 g BW) for six consecutive weeks. (3) Results: Compared with the control group, EC group significantly elevated the serum aspartate aminotransferase (AST), alanine aminitransferase (ALT), and lactate dehydrogenase (LDH). However, GE50 or silymarin treatment effectively reversed these changes. GE50 had a significant protective effect against ethanol + CCl4 induced lipid peroxidation and increased the levels of glutathione (GSH), vitamin C, E, total antioxidant status (TAS), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione S-transferases (GST). Furthermore, in EC focal group, slight fat droplet infiltration was observed in the livers, while in the GE50 or silymarin treatment groups, decreased fat droplet infiltration. HPLC phytochemical profile of GE50 revealed the presence of gallic acid, flavone, genistin, daidzin, and quercetin. (4) Conclusions: The hepatoprotective activity of GE50 is proposed to occur through the synergic effects of its chemical component, namely, gallic acid, flavone, genistin, daidzin, and quercetin. Hence, G. paraguayense can be used as a complementary and alternative therapy in the prevention of alcohol + CCl4-induced liver injury.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

Effects of infliximab against carbon tetrachloride-induced intestinal injury via lipid peroxidation and apoptosis

2019 ◽  
Vol 38 (11) ◽  
pp. 1275-1282
Author(s):  
A Pergel ◽  
L Tümkaya ◽  
MK Çolakoğlu ◽  
G Demiral ◽  
S Kalcan ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Caspase 3 ◽  
Inhalation Exposure ◽  
Isotonic Saline ◽  
Neutrophil Infiltration ◽  
Intestinal Injury ◽  
The Other ◽  
Control Group ◽  
Sprague Dawley ◽  
Other Hand

Carbon tetrachloride (CCL4) is often employed in the production of chlorofluorocarbons, petroleum refining, oil and rubber processing, and laboratory applications. Oral, subcutaneous, and inhalation exposure to CCL4 in animal studies have been shown to be capable of leading to various types of cancer (benign and malignant, liver, breast, and adrenal gland tumors). The present study also evaluated the protective role of infliximab (INF) against the deleterious effects of CCL4 on the intestinal system. Twenty-four male Sprague-Dawley rats were randomly assigned into three groups, control ( n = 8), CCL4 ( n = 8), and CCL4 + INF ( n = 8). The control group received 1 mL isotonic saline solution only via intraperitoneal (i.p.) injection. The CCL4 group received a single i.p. dose of 2 mL/kg CCL4. The CCL4 + INF group received a single i.p. dose of 7 mg/kg INF followed 24 h later by a single dose of 2 mL/kg CCL4. All rats were euthanized 2 days following drug administration. CCL4 group samples also exhibited diffuse loss of enterocytes, vascular congestion, neutrophil infiltration, an extension of the subepithelial space and significant epithelial lifting along the length of the villi with a few denuded villous tips. In addition, CCL4 treatment increased intestinal malondialdehyde (MDA) level and caspase-3 positivity. On the other hand, INF decreased MDA levels, caspase-3 positivity, and loss of villous. Our findings suggest that CCL4 appears to exert a highly deleterious effect on the intestinal mucosa. On the other hand, INF is effective in preventing this CCL4-induced intestinal injury by reducing oxidative stress and apoptosis.

scholarly journals Dietary Plant Extracts Improve the Antioxidant Reserves in Weaned Piglets

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 702
Author(s):  
Carlo Corino ◽  
Michel Prost ◽  
Barbara Pizzi ◽  
Raffaella Rossi
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Whole Blood ◽  
Antioxidant Status ◽  
Biological Activities ◽  
Total Antioxidant Status ◽  
Dietary Treatment ◽  
Control Group ◽  
Commercial Diet ◽  
Total Antioxidant

Reducing the use of antibiotics in livestock in order to contain antibiotic resistance and studying natural substance additives are key to sustainability. Among the various biological activities of plant extracts, antioxidant activity plays an important role. The present study assesses the total antioxidant activity and antioxidant reserves using the Kit Radicaux Libres test (KRL™ Kirial International, Couternon, France). One hundred and sixty piglets (Topics × Tempo) weaned at 28 days of age were divided into four dietary treatment groups that were fed a commercial diet (the control group, C); 500 mg/kg Boswellia extract (BOS); 200 and 50 mg/kg Uncaria and Tanacetum extracts (UT) respectively; and 225 mg/kg of an antioxidant plant extract mixture (AOX). The blood antioxidant activity of the piglets was measured using the KRL test and the reserves were analyzed on whole blood samples after hydrolysis with glucosidase, sulfatase and glucuronidase. No significant differences were observed in growth performance. The delta KRL values of the whole blood showed a significantly higher total antioxidant status of the piglets from the BOS and AOX groups than the UT and C groups (+30.7 BOS; +27.7 AOX vs. +17.81 UT +13.30 C; p = 0.002) between 18 and 28 days post-weaning. The delta KRL values of red blood cells (RBCs) showed a significantly higher total antioxidant status of the piglets from the AOX groups than the UT and BOS groups (+22.2 AOX; vs. +9.90 UT +9.4 BOS; p = 0.016) between the two sampling times. Reserves of UT and AOX were higher than C and BOS for all enzymes, glucosides, sulphates, and glucuronides. The biological KRL test proved to be an extremely sensitive tool to evaluate the piglets’ antioxidant status. Determining the antioxidant reserve also provides a better understanding of the real antioxidant status of pigs.

scholarly journals Efficacy of Aloe vera, Ananas comosus, and Sansevieria masoniana Cream on the Skin Wound Infected with MRSA

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yos Adi Prakoso ◽  
Chylen Setiyo Rini ◽  
Roeswandono Wirjaatmadja
Keyword(s):  
Alternative Therapy ◽  
Aloe Vera ◽  
Herbal Medicines ◽  
Potential Effect ◽  
Punch Biopsy ◽  
Skin Wound ◽  
Ananas Comosus ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

The tropical area has a lot of herbal medicines such as Aloe vera (AV), Ananas comosus (AC), and Sansevieria masoniana (SM). All the three have a unique potential effect as an antibacterial and wound-healing promoter. The aim of this study is to explore the role of AV, AC, and SM on the skin wound infected with methicillin-resistant Staphylococcus aureus (MRSA). Forty-five adult female Sprague Dawley rats weighing 250–300 grams were divided into 5 groups. All the groups were exposed to two round full-thickness punch biopsy and infected with MRSA. The group C was the control group/untreated; group BC was treated with base cream/without extract; group AV was treated with 75% AV cream; group AC was treated with 75% AC cream, and group SM was treated with 75% SM cream. The wounds were observed on days 5, 10, and 15. The healing of skin wounds was measured by a percentage of closure, skin tensile strength, and histopathology. The result showed that AV, AC, and SM have a similar potential effect on healing in the wound that was infected with MRSA compared to the groups C and BC (P<0.05). It shows that all the three herbal formulations can be used as the alternative therapy to the wound infected with MRSA.

A Meta-Analysis of the Efficacy of Acupuncture in Treating Dysphagia in Patients with a Stroke

2012 ◽  
Vol 30 (4) ◽  
pp. 291-297 ◽  
Author(s):  
Yao-Bin Long ◽  
Xiao-Ping Wu
Keyword(s):  
Acupuncture Treatment ◽  
Meta Analysis ◽  
Alternative Therapy ◽  
Cochrane Library ◽  
Control Group ◽  
Complementary And Alternative Therapy ◽  
Dichotomous Data ◽  
Biological Medicine ◽  
The Subject ◽  
Randomised Controlled

Background Dysphagia, or deglutition difficulty, is a common manifestation in patients with a stroke and its management is an important aspect of rehabilitation. Acupuncture, a complementary and alternative therapy, is the subject of growing public interest for treatment of stroke. Objective A meta-analysis was performed to study the effect of acupuncture for treatment of dysphagia in patients affected by a stroke. Methods Randomised controlled trials (RCTs) comparing acupuncture treatment with non-acupuncture treatment of dysphagia in patients with a stroke were identified from the databases of PubMed, Embase, Cochrane Library and CBM disc (China Biological Medicine Database). Eligible investigations were included and data on the effectiveness of acupuncture were extracted and synthesised by meta-analysis using RevMan 5.1.4. Results were expressed as OR for dichotomous data; 95% CIs were also calculated. Results Seventy-two RCTs (3208 patients in the treatment group and 2926 patients in the control group) were identified. Details of randomisation and blinding were not reported and information on withdrawals and dropouts was missing in most of included reports. Meta-analysis showed that the effectiveness of treatment in the group receiving acupuncture was higher than that in the non-acupuncture group (OR=5.17, 95% CI 4.18 to 6.38; p<0.00001). However, the study quality was generally low and of insufficient quality to make recommendations about using acupuncture in the rehabilitation of patients with dysphagia due to stroke. Conclusions Acupuncture might be beneficial in the rehabilitation of patients with dysphagia caused by stroke, and the evidence justifies future high-quality studies.

Hepatoprotective and antioxidant activity of hydromethanolic extract of Daniella oliveri leaves in carbon tetrachloride-induced hepatotoxicity in rats

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Samuel Okwudili Onoja ◽  
Gideon Kelechi Madubuike ◽  
Maxwell Ikechukwu Ezeja
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Negative Control Group ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Control Group ◽  
Photometric Assay ◽  
Dose Dependent Increase ◽  
Dose Dependent

AbstractThe hepatoprotective activity was investigated using carbon tetrachloride (CClThe pretreatment with extract (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) produced a significant (p<0.05) dose-dependent increase in hepatoprotective activity when compared with the negative control group. The extract (25–400 μg/mL concentration) produced a concentration-dependent increase in antioxidant activity in 2, 2-diphenyl-1-picrylhydrazine (DPPH) photometric assay. The ICThe results of the study suggest that

scholarly journals Experimental Evaluation of Prophylactic and Therapeutic Antioxidant Potential of Trimetazidine in Carbon Tetrachloride Induced Oxidative Stress in Rats

2021 ◽  
Author(s):  
Vijay Haribhau Mate ◽  
Vijaya Anil Pandit ◽  
Pradnya Hemant Padalkar ◽  
Chetan Shrirang More ◽  
Kapil S Khade
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Antioxidant Potential ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Group Iii ◽  
Group I

Introduction: Exposure to various drugs and chemicals lead to oxidative stress. Carbon Tetrachloride (CCl4) produces rise in oxidative stress leading to hepatic damage. The drug Trimetazidine (TMZ) shows hepatoprotective activity but its mechanism is not known. The present study would help in establishing antioxidant activity of TMZ as probable mechanism. Aim: To evaluate the antioxidant potential of TMZ in CCl4 induced oxidative stress when given prophylactically/therapeutically in rats. Materials and Methods: An experimental animal study was conducted on 80 adult Wistar rats of either sex (weight-150 to 200 gm) from March 2010 to December 2010 in Bharati Vidyapeeth Medical College, Pune, Maharashtra, India. Randomly, all animals were grouped into 10 equal groups. Group i was normal control (received only water). To induce oxidative stress CCl4 (0.5 mL/kg/d i.p.) was given to all the animals of Group ii to Group x for seven days. The TMZ was given in two doses, TMZ1 (5 mg/kg orally for Group iii and vii) and TMZ2 (10 mg/kg orally for Group iv and viii). Positive standard control (Group v and Group ix) received Liv.52 (1 mL/kg orally). Group vi and Group x received combination of TMZ1 (5 mg/kg orally)+Liv.52 (1 mL/kg orally). Drug treatment was given to animals in group iii, iv, v and vi for 1-14 days (preventive group) and in group vii, viii, ix and x from day 8 to day 14 (therapeutic group). On 15th day, rats were sacrificed and dissected for collection of liver. Part of the livers was homogenised to assess oxidative stress marker enzymes Malondialdehyde (MDA), Superoxide Dismutase (SOD) spectrophotometrically. Statistical analysis was done with one- way Analysis of Variance (ANOVA) followed by post-hoc analysis (Dunnett’s test) using GraphPad Prism 5.0 software. Results: Trimetazidine (5 mg/kg and 10 mg/kg) significantly reduced MDA levels and increased SOD levels when compared with CCl4 treated group suggested antioxidant activity. Combined administration of Liv.52 and TMZ1 also reduced oxidative stress and increased antioxidant activity. Conclusion: Results of the present study suggested that increased oxidative stress was significantly attenuated by drug TMZ in dose dependant manner when compared with the CCl4 group. The antioxidant potential of prophylactic and therapeutic administration of TMZ was comparable. The increased antioxidant effect by Liv.52+TMZ1 combination was only due to the additive antioxidant effects of Liv.52 and TMZ or any other mechanism was involved, needs to be further evaluated.

scholarly journals Effects of Qigong Exercise on Fatigue, Anxiety, and Depressive Symptoms of Patients with Chronic Fatigue Syndrome-Like Illness: A Randomized Controlled Trial

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Jessie S. M. Chan ◽  
Rainbow T. H. Ho ◽  
Chong-wen Wang ◽  
Lai Ping Yuen ◽  
Jonathan S. T. Sham ◽  
...  
Keyword(s):  
Depressive Symptoms ◽  
Chronic Fatigue Syndrome ◽  
Controlled Trial ◽  
Alternative Therapy ◽  
Intervention Group ◽  
Chronic Fatigue ◽  
Control Group ◽  
Complementary And Alternative Therapy ◽  
Fatigue Score ◽  
Fatigue Syndrome

Background. Anxiety/depressive symptoms are common in patients with chronic fatigue syndrome- (CFS-) like illness. Qigong as a modality of complementary and alternative therapy has been increasingly applied by patients with chronic illnesses, but little is known about the effect of Qigong on anxiety/depressive symptoms of the patients with CFS-like illness.Purpose. To investigate the effects of Qigong on fatigue, anxiety, and depressive symptoms in patients with CFS-illness.Methods. One hundred and thirty-seven participants who met the diagnostic criteria for CFS-like illness were randomly assigned to either an intervention group or a waitlist control group. Participants in the intervention group received 10 sessions of Qigong training twice a week for 5 consecutive weeks, followed by home-based practice for 12 weeks. Fatigue, anxiety, and depressive symptoms were assessed at baseline and postintervention.Results. Total fatigue score [F1,135=13.888,P<0.001], physical fatigue score [F1,135=20.852,P<0.001] and depression score [F1,135=9.918,P=0.002] were significantly improved and mental fatigue score [F1,135=3.902,P=0.050] was marginally significantly improved in the Qigong group compared to controls. The anxiety score was not significantly improved in the Qigong group.Conclusion. Qigong may not only reduce the fatigue symptoms, but also has antidepressive effect for patients with CFS-like illness. Trial registrationHKCTR-1200.

scholarly journals Raspberry Polyphenol Extract Decreases NF-kB and IL-6 Expression in Lipopolysaccharide (LPS)-Induced RAW 264.7 Macrophages

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 422-422
Author(s):  
Lena Lear ◽  
Rami Najjar ◽  
Rafaela Feresin ◽  
Jessica Danh
Keyword(s):  
Alternative Therapy ◽  
Control Group ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Complementary And Alternative Therapy ◽  
Funding Sources ◽  
Pre Treatment ◽  
Post Hoc ◽  
Lps Treatment

Abstract Objectives To investigate whether raspberry polyphenol extract attenuates the inflammatory response induced by lipopolysaccharide (LPS) in RAW 264.7 macrophages. Methods Raspberry polyphenol extract was prepared using methanolic extraction, followed by solvent evaporation and freeze-drying. RAW 264.7 macrophages were treated with 0, 50, 100, 200 and 400 μg/ml of raspberry polyphenol extract in six-well plates. After 2 h, cells were then treated with 100 ng/ml of LPS for 6 h. Cells were collected for protein expression analysis of the transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and inflammatory cytokines, i.e., interleukin (IL)-6 and IL-1β, via western blot. Results were analyzed using ANOVA followed by Tukey-Kramer post-hoc test. Results As expected, LPS significantly increased NF-kB compared to control (P &lt; 0.0001). Pre-treatment with 400 μg/ml raspberry polyphenol extract significantly decreased phosphorylation of NF-kB in LPS stimulated cells (0.71 ± 0.03 vs. 1.00 ± 0.00-fold, P = 0.005) compared to LPS alone. LPS treatment significantly increased the expression of IL-6 compared to the control group (P &lt; 0.0001). IL-6 expression was significantly reduced in LPS stimulated macrophages by pre-treatment with 200 and 400 µg/ml of raspberry polyphenol extract (0.49 ± 0.03-fold, P &lt; 0.0001 and 0.33 ± 0.04-fold, P &lt; 0.0001 respectively) compared to LPS alone (1.00 ± 0.00-fold). The expression of the inflammatory cytokine IL-1β was significantly greater than control in LPS-only treated cells (P &lt; 0.0001). However, treatment with 400 μg/ml raspberry polyphenol was able to significantly prevent this effect (0.59 ± 0.1 vs. 1.00 ± 0.00-fold, P = 0.007). Conclusions Results indicate that raspberry polyphenols possess anti-inflammatory properties suggesting a possible role as a complementary and alternative therapy to prevent inflammation. However, in vivo and human studies are needed to confirm this. Funding Sources None.

scholarly journals Mechanism of antidiabetic effects of Plicosepalus Acaciae flower in streptozotocin-induced type 2 diabetic rats, as complementary and alternative therapy

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Mohamed-I Kotb El-Sayed ◽  
Shaza Al-Massarani ◽  
Ali El Gamal ◽  
Amina El-Shaibany ◽  
Hassan M Al-Mahbashi
Keyword(s):  
Blood Glucose ◽  
Alternative Therapy ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Dependent Manner ◽  
Complementary And Alternative Therapy ◽  
Tnf Α ◽  
Complementary And Alternative

Abstract Background Diabetes and its related complications remain to be a major clinical problem. We aim to investigate the antidiabetic mechanistic actions of Plicosepalus Acaciae (PA) flowers in streptozotocin (STZ)-induced diabetic rats. Methods After diabetes induction, rats were divided randomly into five groups, including: 1) normal control group, 2) diabetic control group, 3) diabetic group treated with 150 mg/kg of ethanolic extract of PA flowers, 4) diabetic group treated with 300 mg/kg of ethanolic extract of PA flowers, and 5) diabetic group treated with 150 mg/kg of metformin. After 15 days of treatment; fasting blood glucose, glycated hemoglobin (HBA1c%), insulin, C-peptide, superoxide dismutase (SOD), catalase, reduced glutathione (GSH), malondialdehyde (MDA), triglyceride (TGs), total cholesterol (Tc), low density lipoprotein cholesterol (LDL-c), very LDL (VLDL), high DLc (HDL-c), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels were assessed. Histopathology of pancreas was also assessed. Results The results showed that PA flower ethanolic extract significantly reduced blood glucose, HBA1c%, MDA, TGs, Tc, VLDL, LDL-c, TNF-α, and IL-6 levels in a dose-dependent manner. All these parameters were already increased by diabetic induction in the untreated diabetic group. Treatment of diabetic rats with PA flower increased insulin, HDL-c, GSH, catalase, and SOD levels. Histological examination showed that the PA flower caused reconstruction, repair, and recovery of damaged pancreas when compared with the untreated group. Conclusions PA flower has a potential role in the management of diabetes as complementary and alternative therapy, due to its antioxidant, anti-inflammatory, hypolipidemic, hypoglycemic and insulin secretagogue effects.

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