scholarly journals Production of a Novel Functional Fruit Beverage Consisting of Cornelian Cherry Juice and Probiotic Bacteria

Antioxidants ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 163 ◽  
Author(s):  
Ioanna Mantzourani ◽  
Chryssa Nouska ◽  
Antonia Terpou ◽  
Athanasios Alexopoulos ◽  
Eugenia Bezirtzoglou ◽  
...  
Keyword(s):  
Storage Time ◽  
Immobilized Cells ◽  
Culture Collection ◽  
Total Phenolic ◽  
Cornelian Cherry ◽  
Free Cells ◽  
Cherry Juice ◽  
Phenolics Content ◽  
Low Levels ◽  
Fermented Juice

The present study describes the development of a novel functional beverage through the application of probiotic Lactobacillus plantarum ATCC (American Type Culture Collection) 14917 in Cornelian cherry juice fermentation. The probiotic was employed in free and immobilized in a delignified wheat bran carrier (DWB) form. Cornelian cherry juice was fermented for 24 h and then it was stored at 4 °C for 4 weeks. Several parameters were evaluated such as residual sugar, organic acid and alcohol levels, total phenolics content, and cell viability as well as consumers acceptance. Regarding sugar and organic acids analyses, it was proved that the probiotic free or immobilized biocatalyst was effective. The concentration of ethanol was maintained at low levels (0.3–0.9% v/v). The total phenolic content of fermented Cornelian cherry juice with immobilized cells was recorded in higher levels (214–264 mg GAE/100 mL) for all the cold storage time compared to fermented juice with free cells (165–199 mg GAE/100 mL) and non-fermented juice (135–169 mg GAE/100 mL). Immobilized cells retained their viability in higher levels (9.95 log cfu/mL at the 4th week) compared to free cells (7.36 log cfu/mL at the 4th week). No significant sensory differences were observed among the fermented and the non-fermented samples.

scholarly journals Potential of the Probiotic Lactobacillus Plantarum ATCC 14917 Strain to Produce Functional Fermented Pomegranate Juice

Foods ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 4 ◽  
Author(s):  
Ioanna Mantzourani ◽  
Stavros Kazakos ◽  
Antonia Terpou ◽  
Athanasios Alexopoulos ◽  
Eugenia Bezirtzoglou ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Probiotic Strain ◽  
Pomegranate Juice ◽  
Total Phenolic ◽  
Acid Fermentation ◽  
Research Survey ◽  
Trolox Equivalent ◽  
Low Levels ◽  
And Storage ◽  
Fermented Juice

In this research survey the application of probiotic strain Lactobacillus plantarum ATCC 14917 in pomegranate juice fermentation is sought. Pomegranate juice was fermented for 24 h and then it was stored 4 for 4 weeks. Cell viability retained in high levels after the 24 h of fermentation and storage for 4 weeks (above 8.8 log cfu/mL), while fermented pomegranate juice was scored better at the 4th week of storage compared to non-fermented pomegranate juice. The probiotic strain was effective regarding lactic acid fermentation as was proved through sugar and organic acids analysis. Concentration of ethanol was maintained at low levels (0.3–1% v/v). Fermented pomegranate juice contained more and in higher percentages desirable volatile compounds (alcohols, ketones and esters) even at the 4th week of cold storage compared to non-fermented juice. Antioxidant activity (150.63 mg Trolox equivalent (TE)/100 mL at the 2nd week) and total phenolic content (206.46 mg gallic acid equivalents (GAE)/100 mL at the 2nd week) were recorded in higher levels for all the storage time compared to non-fermented juice.

scholarly journals Potential Application of Cornelian Cherry Extract on Broiler Chickens: Growth, Expression of Antioxidant Biomarker and Glucose Transport Genes, and Oxidative Stability of Frozen Meat

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1038
Author(s):  
Doaa Ibrahim ◽  
Amira Moustafa ◽  
Aya Sh. Metwally ◽  
Mohamed A. Nassan ◽  
Karima Abdallah ◽  
...  
Keyword(s):  
Oxidative Stability ◽  
Broiler Chickens ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Poultry Feed ◽  
Total Phenolic ◽  
Control Group ◽  
Breast Meat ◽  
Antioxidant Genes ◽  
Cornelian Cherry

The use of natural plant extracts in poultry feed could improve their productivity as well as the oxidative stability of stored derived meat. The roles of cornelian cherry extract (CCE) in growth, cecal microbes, and meat antioxidative markers of broiler chickens were evaluated. A total of 500 Ross 308 broiler chicks were fed diets supplemented with CCE (0, 50, 100, 200, 400 mg/kg of diet) for 38 days. The highest levels of weight gain and feed utilization were observed in a group fed 200 mg/kg of CCE. Maximum upregulation of glucose transporters—1 and 2 and sodium-dependent glucose transporter genes—were found in the group fed 200 mg/kg of CCE. Lactobacilli and Bifidobacterium colonization increased as the CCE levels increased. The greatest upregulation of antioxidant genes (glutathione peroxidase, catalase, and superoxide dismutase) in breast meat was observed in groups fed CCE (200 and 400 mg/kg). Dietary CCE significantly delayed the lipid oxidation of breast meat compared with that of the control group. The total phenolic content, 2,2-Diphenyl-1-Picrihydrzyl (DPPH) radical scavenging activity and reducing power in meat improved with higher levels of CCE. Dietary CCE improved the growth, performance of broilers, and meat antioxidant stability after 90 days of storage.

Semicontinuous and Continuous Production of Citric Acid with Immobilized Cells of Aspergillus niger

1987 ◽  
Vol 42 (4) ◽  
pp. 408-413 ◽  
Author(s):  
H. Eikmeier ◽  
H. J. Rehm
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Immobilized Cells ◽  
Continuous Production ◽  
Continuous Process ◽  
Batch Experiments ◽  
Medium Exchange ◽  
Free Cells ◽  
Semicontinuous Cultivation ◽  
Ca Alginate

Abstract The citric acid excretion of Ca-alginate-immobilized cells of Aspergillus niger in batch culture decreased with a half-time of approximately 19 days. Reactivation of the biocatalysts by regeneration in growth medium was possible, but it was followed by a submerged sporulation of the fungus, and medium was highly contaminated with free cells. Citric acid production could better be prolonged by semicontinuous cultivation with medium exchange every 7 or 14 days, respectively. After 32 days the remaining activity in semicontinuous culture was 1.4-fold higher than in comparable batch experiments. Similar improvements were obtained with a continuous process at a dilution rate of 0.125 v/v · d, whereby medium efflux completely free of detaching mycelia.

scholarly journals Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2771 ◽  
Author(s):  
Mohammed Umar Mustapha ◽  
Normala Halimoon ◽  
Wan Lutfi Wan Johari ◽  
Mohd. Yunus Abd Shukor
Keyword(s):  
Agricultural Soil ◽  
Immobilized Cells ◽  
Free Cell ◽  
Gellan Gum ◽  
Suspended Cell ◽  
Rainfall Events ◽  
Free Cells ◽  
Degradation Activity ◽  
Freely Suspended ◽  
Suspended Cells

Extensive use of carbofuran insecticide harms the environment and human health. Carbofuran is an endocrine disruptor and has the highest acute toxicity to humans than all groups of carbamate pesticides used. Carbofuran is highly mobile in soil and soluble in water with a lengthy half-life (50 days). Therefore, it has the potential to contaminate groundwater and nearby water bodies after rainfall events. A bacterial strain BRC05 was isolated from agricultural soil characterized and presumptively identified as Enterobacter sp. The strain was immobilized using gellan gum as an entrapment material. The effect of different heavy metals and the ability of the immobilized cells to degrade carbofuran were compared with their free cell counterparts. The results showed a significant increase in the degradation of carbofuran by immobilized cells compared with freely suspended cells. Carbofuran was completely degraded within 9 h by immobilized cells at 50 mg/L, while it took 12 h for free cells to degrade carbofuran at the same concentration. Besides, the immobilized cells completely degraded carbofuran within 38 h at 100 mg/L. On the other hand, free cells degraded the compound in 68 h. The viability of the freely suspended cell and degradation efficiency was inhibited at a concentration greater than 100 mg/L. Whereas, the immobilized cells almost completely degraded carbofuran at 100 mg/L. At 250 mg/L concentration, the rate of degradation decreased significantly in free cells. The immobilized cells could also be reused for about nine cycles without losing their degradation activity. Hence, the gellan gum-immobilized cells of Enterobacter sp. could be potentially used in the bioremediation of carbofuran in contaminated soil.

scholarly journals Antioxidant Capacity, Total Phenols, Total Flavonoids and Colour Component of Cornelian Cherry (Cornus mas L.) Wild Genotypes

2018 ◽  
Vol 47 (2) ◽  
pp. 390-394 ◽  
Author(s):  
Sina N. COSMULESCU ◽  
Ion TRANDAFIR ◽  
Felicia CORNESCU
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Blue Color ◽  
Total Phenols ◽  
Cornelian Cherry ◽  
Color Component ◽  
Cornus Mas

A number of selected cornelian cherry wild genotypes were investigated regarding antioxidant capacity, total phenols, total flavonoids and colour component. The total phenolic, total flavonoids and antioxidant activity of methanol extract of cornelian cherry fruits were determined using Folin-Ciocalteu, colorimetric and DPPH methods, respectively. Total phenolic content ranged from 163.69 (S1) to 359.28 (H2) mg GAE 100 g-1 FW. Genotypes H2 and H3 had the highest total phenolic content (359.28 and 343.50 mg GAE 100 g-1 FW, respectively), total flavonoids (54.26 and 64.48 mg QE 100 g-1 FW, respectively) and antioxidant capacity (2.39 and 2.71 mmolTrolox100g-1 FW, respectively). Significant differences of yellow/blue color component (b*), red/ green (a*) color component, values of lightness (L*) and chroma (C) were obtained for extracts from fruits. The present study shows the potential of certain cornelian cherry genotypes as valuable source of natural antioxidants and gene stock for breeding programs.

Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

Biologia ◽  
2010 ◽  
Vol 65 (3) ◽  
Author(s):  
Shuvashish Behera ◽  
Rama Mohanty ◽  
Ramesh Ray
Keyword(s):  
Calcium Alginate ◽  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Dry Weight ◽  
Weight Basis ◽  
Deciduous Tree ◽  
Madhuca Latifolia ◽  
Australian Continent ◽  
Free Cells ◽  
Alginate Matrix

AbstractMahula (Madhuca latifolia L.) is a deciduous tree commonly found in the tropical rain forests of Asian and Australian continent. Corolla, the edible part of its flowers, is rich in fermentable sugar (37 ± 0.23%; on dry weight basis). Batch fermentation of mahula flowers was carried out using Zymomonas mobilis MTCC 92 free cells and cells immobilized in calcium alginate matrix. The ethanol productions were 122.9 ± 0.972 and 134.6 ± 0.104 g/kg flowers on dry weight basis using free and immobilized cells, respectively, after 96 h of fermentation, which showed that cells entrapped in calcium alginate matrix yielded 8.7% more ethanol than free cells. Further, the immobilized cells were physiologically active up to three more cycles of fermentation producing 132.7 ± 0.095, 130.5 ± 0.09 and 128.7 ± 0.056 g ethanol per kg flower in first, second and third cycle, respectively.

scholarly journals Immobilization of Pseudomonas sp. KLM9 in Sodium Alginate: A Promising Technique for L-Glutaminase Production

2015 ◽  
Vol 31 ◽  
pp. 27-35 ◽  
Author(s):  
Divatar Mahesh ◽  
G. Sandhya ◽  
Ahemad Sarfraaz ◽  
K. Lingappa
Keyword(s):  
Sodium Alginate ◽  
Good Yield ◽  
Process Development ◽  
Incubation Temperature ◽  
Immobilized Cells ◽  
Moderate Increase ◽  
Promising Technique ◽  
Free Cells ◽  
Initial Ph ◽  
Fermentation Period

Microbes and process development are gaining considerable importance owing to their exploitation and suitability for the production of the enzymes of therapeutic and industrial significance. In the present study attempts were made to immobilize the Pseudomonas Sp. KLM9 in different supporting matrix to analyze the possible enhancement in the production of L-Glutaminase. The consequences of the study reveal that, sodium alginate at 3.5% concentration was found to be the better supporting matrix than the others. Further, initial pH 7 of the incubation media, incubation temperature of 37°C were supported good yield of L-Glutaminase by immobilized Pseudomonas Sp KLM9. The fermentation period was reduced to 36 hr with immobilized cells. There is moderate increase in the production L-Glutaminase by the immobilized cells than the free cells of Pseudomonas Sp KLM9. Biomass obtained from the 18 hr old culture of Pseudomonas Sp KLM9 was found better to immobilize for the production of L-Glutaminase.

scholarly journals Response of Pseudokirchneriella subcapitata in Free and Alginate Immobilized Cells to Heavy Metals Toxicity

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2847
Author(s):  
Zaki M. Al-Hasawi ◽  
Mohammad I. Abdel-Hamid ◽  
Adel W. Almutairi ◽  
Hussein E. Touliabah
Keyword(s):  
Heavy Metals ◽  
Toxic Metals ◽  
Immobilized Cells ◽  
Algal Growth ◽  
Pseudokirchneriella Subcapitata ◽  
Response Curves ◽  
Free Cells ◽  
Heavy Metals Toxicity ◽  
Removal Of Heavy Metals ◽  
Immobilized Algae

Effects of 12 heavy metals on growth of free and alginate-immobilized cells of the alga Pseudokirchneriella subcapitata were investigated. The tested metals ions include Al, As, Cd, Co, Cr, Cu, Hg, Se, Ni, Pb, Sr, and Zn. Toxicity values (EC50) were calculated by graphical interpolation from dose-response curves. The highest to the lowest toxic metals are in the order Cd > Co > Hg > Cu > Ni > Zn > Cr > Al > Se > As > Pb > Sr. The lowest metal concentration (mg L−1) inhibiting 50% (EC50) of algal growth of free and immobilized (values in parentheses) algal cells were, 0.018 (0.09) for Cd, 0.03 (0.06) for Co, 0.039 (0.06) for Hg, 0.048 (0.050) for Cu, 0.055 (0.3) for Ni, 0.08 (0.1) for Zn, 0.2 (0.3) for Cr, 0.75 (1.8) for Al, 1.2 (1.4) for Se, 3.0 (4.0) for As, 3.3 (5.0) for Pb, and 160 (180) for Sr. Free and immobilized cultures showed similar responses to Cu and Se. The free cells were more sensitive than the immobilized ones. Accordingly, the toxicity (EC50) of heavy metals derived only form immobilized algal cells might by questionable. The study suggests that batteries of alginate-immobilized algae can efficiently replace free algae for the bio-removal of heavy metals.

Biotransformation of Monoterpenes by Polyurethane Foam-Immobilized Cells of Petroselinum crispum (Mill) Nyman

1990 ◽  
Vol 45 (3-4) ◽  
pp. 245-248 ◽  
Author(s):  
A. A. Gbolade ◽  
G. B. Lockwood
Keyword(s):  
Polyurethane Foam ◽  
Immobilized Cells ◽  
Plant Cells ◽  
Petroselinum Crispum ◽  
Growth Limitation ◽  
Biochemical Reactions ◽  
Free Cells ◽  
Freely Suspended ◽  
Suspended Cells

Abstract Cells of Petroselinum crispum cv. “Paramount” and “Plain-leaved” immobilized in polyurethane foam retained the biotransformational characteristics of their freely suspended cells for certain monoterpenic substrates. Immobilized cells of both cultivars were more efficient than their free cells in isomerizing geraniol into nerol, but less efficient (<60% ) in reducing the aldehydes, citral and citronellal. Maintenance of the immobilized cells under the condition of growth limitation did not enhance the efficiency of the biochemical reactions. Under the same condition, the freely suspended cells of cult. “Paramount” exhibited higher efficiency (up to 1.6 times) of biotransformation of citral when compared to those in sucrose-supplemented media. This investigation lends support to the view that the biochemical productivity of cultured plant cells for especially monoterpenoids may not necessarily be improved upon immobilization.

Textile Effluent Discoloration by Immobilized Phanerochaete Chrysosporium into PVA-Alginate-Sulfate Beads

2013 ◽  
Vol 62 (2) ◽  
Author(s):  
Nor Atikah Husna Ahmad Nasir ◽  
Nor Fadhilatul Shilla Mohd Asri ◽  
Nor Azimah Mohd Zain ◽  
Mohd Suardi Suhaimi ◽  
Ani Idris
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Manganese Peroxidase ◽  
Immobilized Cells ◽  
Free Cell ◽  
Cod Removal ◽  
Immobilized Cell ◽  
Colour Removal ◽  
Free Cells ◽  
Immobilization Matrix ◽  
Preliminary Research

This paper presents preliminary research on immobilized Phanerochaete chrysosporium in PVA-alginate-sulfate beads to discolor textile effluents. It is an alternative technique from the current physico-chemicals. The main focus of this study was to determine the colour removal, Chemical oxygen deman (COD) removal and manganese peroxidase activity of the immobilized P.crysosporium. Immobilized P.crysosporium also confers advantages such as reusability and improved cell performance. Scanning electron microscope (SEM) was also performed to characterize the immobilization matrix. The immobilized results were compared with that of free cells. Immobilized cells were able to discolor 47.14% compared to free cells which recorded 10.78% colour removal. The COD removal of immobilized cell is more than 60% as compared to that of free cells, which could only reduced 30% of COD. Finally, the manganase peroxidase activities showed a slight difference between the immobilized and free cell at 0.15U/L and 0.13U/L respectively.

Sign in / Sign up

Export Citation Format

Share Document