scholarly journals Reactive Oxygen and Nitrogen Species Regulate Key Metabolic, Anabolic, and Catabolic Pathways in Skeletal Muscle

Antioxidants ◽  
2018 ◽  
Vol 7 (7) ◽  
pp. 85 ◽  
Author(s):  
Roland Nemes ◽  
Erika Koltai ◽  
Albert Taylor ◽  
Katsuhiko Suzuki ◽  
Ferenc Gyori ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Reactive Oxygen ◽  
Nitrogen Species ◽  
Catabolic Pathways

The production of reactive oxygen and nitrogen species by skeletal muscle

2007 ◽  
Vol 102 (4) ◽  
pp. 1664-1670 ◽  
Author(s):  
Malcolm J. Jackson ◽  
Deborah Pye ◽  
Jesus Palomero
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Potential Role ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Redox Biology ◽  
Controlled Systems ◽  
Potential Source

Skeletal muscle has been recognized as a potential source for generation of reactive oxygen and nitrogen species for more than 20 years. Initial investigations concentrated on the potential role of mitochondria as a major source for generation of superoxide as a “by-product” of normal oxidative metabolism, but recent studies have identified multiple subcellular sites, where superoxide or nitric oxide are generated in regulated and controlled systems in response to cellular stimuli. Full evaluation of the factors regulating these processes and the functions of the reactive oxygen species generated are important in understanding the redox biology of skeletal muscle.

scholarly journals Reactive oxygen/nitrogen species and contractile function in skeletal muscle during fatigue and recovery

2016 ◽  
Vol 594 (18) ◽  
pp. 5149-5160 ◽  
Author(s):  
Arthur J. Cheng ◽  
Takashi Yamada ◽  
Dilson E. Rassier ◽  
Daniel C. Andersson ◽  
Håkan Westerblad ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Contractile Function ◽  
Reactive Oxygen ◽  
Nitrogen Species

Release of reactive oxygen and nitrogen species from contracting skeletal muscle cells

2004 ◽  
Vol 37 (7) ◽  
pp. 1064-1072 ◽  
Author(s):  
David M. Patwell ◽  
Anne McArdle ◽  
Jennifer E. Morgan ◽  
Terence A. Patridge ◽  
Malcolm J. Jackson
Keyword(s):  
Skeletal Muscle ◽  
Reactive Oxygen ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Nitrogen Species

scholarly journals Genetically Encoded Biosensors to Monitor Intracellular Reactive Oxygen and Nitrogen Species and Glutathione Redox Potential in Skeletal Muscle Cells

2021 ◽  
Vol 22 (19) ◽  
pp. 10876
Author(s):  
Escarlata Fernández-Puente ◽  
Jesús Palomero
Keyword(s):  
Skeletal Muscle ◽  
Hydrogen Peroxide ◽  
Fluorescence Microscopy ◽  
Redox Potential ◽  
Reactive Oxygen ◽  
Nitrogen Species ◽  
Microscopy Imaging ◽  
Redox Biology ◽  
Quantitative Fluorescence ◽  
Glutathione Redox

Reactive oxygen and nitrogen species (RONS) play an important role in the pathophysiology of skeletal muscle and are involved in the regulation of intracellular signaling pathways, which drive metabolism, regeneration, and adaptation in skeletal muscle. However, the molecular mechanisms underlying these processes are unknown or partially uncovered. We implemented a combination of methodological approaches that are funded for the use of genetically encoded biosensors associated with quantitative fluorescence microscopy imaging to study redox biology in skeletal muscle. Therefore, it was possible to detect and monitor RONS and glutathione redox potential with high specificity and spatio-temporal resolution in two models, isolated skeletal muscle fibers and C2C12 myoblasts/myotubes. Biosensors HyPer3 and roGFP2-Orp1 were examined for the detection of cytosolic hydrogen peroxide; HyPer-mito and HyPer-nuc for the detection of mitochondrial and nuclear hydrogen peroxide; Mito-Grx1-roGFP2 and cyto-Grx1-roGFP2 were used for registration of the glutathione redox potential in mitochondria and cytosol. G-geNOp was proven to detect cytosolic nitric oxide. The fluorescence emitted by the biosensors is affected by pH, and this might have masked the results; therefore, environmental CO2 must be controlled to avoid pH fluctuations. In conclusion, genetically encoded biosensors and quantitative fluorescence microscopy provide a robust methodology to investigate the pathophysiological processes associated with the redox biology of skeletal muscle.

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