scholarly journals Polyphenolic Composition and Antioxidant Activity of Aqueous and Ethanolic Extracts from Uncaria tomentosa Bark and Leaves

Antioxidants ◽  
2018 ◽  
Vol 7 (5) ◽  
pp. 65 ◽  
Author(s):  
Mirtha Navarro-Hoyos ◽  
Diego Alvarado-Corella ◽  
Ileana Moreira-Gonzalez ◽  
Elizabeth Arnaez-Serrano ◽  
Maria Monagas-Juan
Keyword(s):  
Antioxidant Activity ◽  
Uncaria Tomentosa ◽  
Polyphenolic Composition ◽  
Ethanolic Extracts

Antioxidant activity and polyphenolic composition of water knotweed (Polygonum amphibium L.) ethanolic extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
E Svirčev ◽  
K Balog ◽  
M Lesjak ◽  
N Mimica-Dukic ◽  
D Orcic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Polyphenolic Composition ◽  
Ethanolic Extracts

scholarly journals Polyphenolic Composition and Antioxidant Activity of Uncaria tomentosa Commercial Bark Products

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 339 ◽  
Author(s):  
Mirtha Navarro ◽  
Elizabeth Arnaez ◽  
Ileana Moreira ◽  
Alonso Hurtado ◽  
Daniela Monge ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Costa Rica ◽  
Biological Activities ◽  
Pearson Correlation ◽  
Commercial Products ◽  
Uncaria Tomentosa ◽  
Average Value ◽  
Polyphenolic Composition ◽  
Significant Difference ◽  
Polyphenolic Profile

Uncaria tomentosa, which is widely commercialized as an herbal medicine, constitutes an important source of secondary metabolites with diverse biological activities. For instance, we have previously reported, for the first time, of a polyphenolic profile rich in proanthocyanidins from extracts of U. tomentosa plants, as well as their antioxidant capacity, antimicrobial activity on aerial bacteria, and cytotoxicity on cancer cell lines. These promising results prompted this research to evaluate the polyphenolic contents of U. tomentosa commercial products. We report a detailed study on the polyphenolic composition of extracts from U. tomentosa bark products (n = 18) commercialized in Costa Rica and Spain. Using HPLC-DAD/TQ-ESI-MS, a total of 25 polyphenolic compounds were identified, including hydroxybenzoic and hydroxycinnamic acids, flavan-3-ol monomers, procyanidin dimers, procyanidin trimers, as well as propelargonidin dimers. Our findings on the polyphenolic profile for all commercial samples show analogous composition to previous reports on U. tomentosa bark material, for instance a 41–49% content of procyanidin dimers and the presence of propelargonidin dimers (8–15%). However, most of the 18 commercial samples exhibit low proanthocyanidin contents (254.8–602.8 µg/g), more similar to previous U. tomentosa inner bark reports, while some exhibit better results, with one sample (SP-2) showing the highest contents (2386.5 µg/g) representing twice the average value of all 18 commercial products. This sample also exhibits the highest total phenolics (TP) and total proanthocyanidins (PRO) contents, as well as the highest Oxygen Radical Absorbance Capacity (ORAC) value (1.31 µg TE/g). One-way Analysis of Variance (ANOVA) with a Tukey post hoc test indicated significant difference (p < 0.05) between products from Costa Rica and Spain for TP and PRO findings, with samples from Spain exhibiting a higher average value. In addition, Pearson correlation analysis results showed a positive correlation (p < 0.05) between TP, PRO, and ORAC results, and an especially important correlation between ORAC antioxidant values and procyanidin dimers (r = 0.843, p < 0.05), procyanidin trimers (r = 0.847, p < 0.05), and propelargonidin dimers (r = 0.851, p < 0.05) contents. Finally, Principal Component Analysis (PCA) results indicated some variability in the composition regardless of their origin. However, only one sample (SP-2) stands out significatively, showing the highest PC1 because of its particularly high proanthocyanidins contents, which could be attributed to the 15% bark polyphenolic extract labeled in this commercial product, which differentiate this sample from all other 17 commercial samples. Therefore, our findings confirmed previous results on the value of extracts in the elaboration of potential commercial products from U. tomentosa, rich in proanthocyanidins and exhibiting high antioxidant activity.

scholarly journals Phytochemical analysis and antioxidant activity of some thymus species from Romania

2021 ◽  
Vol 14 (1) ◽  
pp. 27-33
Author(s):  
Florina Căpățină ◽  
◽  
Eva Suciu ◽  
Daniela Benedec ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Phytochemical Analysis ◽  
Chromatography Method ◽  
Aerial Parts ◽  
Polyphenolic Composition ◽  
Dpph Method ◽  
Ethanolic Extracts ◽  
Layer Chromatography

Objectives. Thymus species, T. vulgaris, T. serpyllum, T. comosus and T. glabrescens, are medicinal plants from the spontaneous Romanian flora used for their antibacterial, antispasmodic, expectorant, anti-inflammatory, anthelmintic properties, especially the plant products obtained from T. vulgaris. The aim of this study was to comparatively investigate the polyphenolic composition, as well as the in vitro antioxidant activity of some ethanolic extracts obtained from the aerial parts of thymus sp. Materials and methods. The polyphenols, flavonoids, phenolic acids and tannins contents in the four samples thymus sp. ethanolic extracts were spectrophotometrically determined. The identification of the most important polyphenolic compounds was performed by the method of thin layer chromatography method (TLC). The antioxidant activity of hydroalcoholic extracts was evaluated in vitro by the DPPH method. Outcomes. Following the analyzes performed, it can be revealed that there are both qualitative and quantitative differences in the polyphenolic composition of the four species of thyme studied, the richest in active principles being T. vulgaris and T. serpyllum. Conclusions. In this paper it has been shown that thyme species are medicinal plants rich in antioxidant polyphenolic active principles, and their use for the treatment of certain diseases can be justified. In addition to the officinal species: T. vulgaris, T. serpyllum, other thyme species from the spontaneous flora of our country can be used in phytotherapy, such as: T. comosus, T. glabrescens.

scholarly journals Changes in Human Erythrocyte Membrane Exposed to Aqueous and Ethanolic Extracts from Uncaria tomentosa

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3189
Author(s):  
Piotr Duchnowicz ◽  
Radosław Pilarski ◽  
Jaromir Michałowicz ◽  
Bożena Bukowska
Keyword(s):  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Ethanolic Extract ◽  
Blood Purification ◽  
Human Erythrocyte Membrane ◽  
Uncaria Tomentosa ◽  
Erythrocyte Shape ◽  
Ethanolic Extracts ◽  
Erythrocyte Membrane Fluidity ◽  
Erythrocyte Plasma Membrane

Uncaria tomentosa (Willd.) DC is a woody climber species originating from South and Central America that has been used in the therapy of asthma, rheumatism, hypertension, and blood purification. Our previous study showed that U. tomentosa extracts altered human erythrocyte shape, which could be due to incorporation of the compounds contained in extracts into the erythrocyte membrane. The aim of the present study was to determine how the compounds contained in U. tomentosa extracts incorporate into the human erythrocyte membrane. The study has assessed the effect of aqueous and ethanolic extracts from leaves and bark of U. tomentosa on the osmotic resistance of the human erythrocyte, the viscosity of erythrocyte interior, and the fluidity of erythrocyte plasma membrane. Human erythrocytes were incubated with the studied extracts in the concentrations of 100, 250, and 500 µg/mL for 2, 5, and 24 h. All extracts tested caused a decrease in erythrocyte membrane fluidity and increased erythrocyte osmotic sensitivity. The ethanolic extracts from the bark and leaves increased viscosity of the erythrocytes. The largest changes in the studied parameters were observed in the cells incubated with bark ethanolic extract. We consider that the compounds from U. tomentosa extracts mainly build into the outer, hydrophilic monolayer of the erythrocyte membrane, thus protecting the erythrocytes against the adverse effects of oxidative stress.

scholarly journals Utero-relaxing and anti-free-radical effects of hydro-ethanolic extracts of Desmodium ramosissimum in the wistar female rats

2021 ◽  
Vol 2 ◽  
pp. 6
Author(s):  
Hèzouwè Madjalani ◽  
Kossi Metowogo ◽  
Tcha Pakoussi ◽  
Aklesso Nabede ◽  
Essodjolon Kanabiya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Medicinal Plants ◽  
Rural Areas ◽  
Public Health Problem ◽  
Stem Bark ◽  
Sub Saharan Africa ◽  
Female Rats ◽  
Phenolic Constituents ◽  
Ethanolic Extracts

Objectives: In sub-Saharan Africa, the therapeutic use of medicinal plants is of arousing interest. Reproductive disorders are a public health problem like everywhere else in the world. The population, especially in rural areas, uses medicinal plants for their beneficial effects. In Africa, the population uses these medicinal plants for their healthcare, including solving problems related to childbirth where there is an increase in the number of women known as “traditional birth attendants.” The effects of its medicinal plants relate to the various reproductive processes in women, from pregnancy to induction of labor, elimination of placental retention, and management of postpartum hemorrhage. To contribute to the enhancement of medicinal plants traditionally used in reproductive healthcare in Togo and to make information on their pharmacological effects available to the population, this work focused on the evaluation utero-relaxing and anti-free radical effects of extracts of Desmodium ramosissimum in the female rats of the Wistar strain. Material and Methods: The effects of hydro-ethanolic extracts of leaves, stem barks, and roots of D.ramosissimum were then tested on shreds Myometer of the female rats mounted in an organ vat and the antioxidant activity of these extracts tested was then determined. Results: The results show that hydro-ethanolic extracts from leaves, stem bark, and roots of D.ramosissimum cause a reduction in the frequency of spontaneous contractions of the uterine muscle. This reduction shows that these extracts have relaxing effects on the contraction of this muscle. The leaves extract showed better antioxidant activity compared to other extracts. Conclusion: This antioxidant activity is probably due to the phenolic constituents and its metabolites would be partly responsible for the pharmacological activities of D.ramosissimum. D.ramosissimum could thus be used to reduce uterine contractions in order to avoid Threats of Premature Labor.

scholarly journals Evaluation of the Antioxidant Activity of Ethanolic Extracts of Some Varieties of Onions

2014 ◽  
Vol 03 (02) ◽  
pp. 26-34
Author(s):  
María del Carmen Gutierrez ◽  
Patricia Della Rocca ◽  
Elizabeth De Seta ◽  
Fernando Reina
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extracts

scholarly journals Prunus persica var. platycarpa (Tabacchiera Peach): Bioactive Compounds and Antioxidant Activity of Pulp, Peel and Seed Ethanolic Extracts

2015 ◽  
Vol 70 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Monica R. Loizzo ◽  
Deborah Pacetti ◽  
Paolo Lucci ◽  
Oscar Núñez ◽  
Francesco Menichini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Prunus Persica ◽  
Ethanolic Extracts

Mycochemical composition and antioxidant potential of Macrolepiota africana (Heim) Heinem. (Agaricaceae), an edible mushroom from Gabon

2021 ◽  
Vol 2 (1) ◽  
pp. 028-033
Author(s):  
Hugues Calixte Eyi Ndong ◽  
André Ledoux Njouonkou
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Activity Index ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Edible Mushroom ◽  
Mushroom Species ◽  
Chemical Screening ◽  
Ethanolic Extracts ◽  
Pharmacological Potential

Macrolepiota africana is a fungus used as food in Gabon for its culinary properties. However, its nutritional value, mycochemical contents, antioxidant properties and health potential still unknown. The present study investigated the phytochemicals and antioxidant properties of this mushroom species. This chemical screening was followed by a study of the antioxidant activity and a prediction of additional pharmacological activities of M. africana. Using standard methodology, the mycochemical analyses were carried out on aqueous, hydro-ethanolic and ethanolic fungi extracts. The antioxidant activity of the mushroom extracts was determined using DPPH radical scavenging assay. Apart from Digitoxigenine, flavonol and gitoxigenine that were not found in any of the extracts, all other tested mycochemical were found in atleast one of the extracts. Alkaloids, flavonoids, polyphenols, oses and holosides, proanthocyanidins and coumarins were found in all extracts at different intensity. Saponosids, sterols and triterpenes, tannins gallics, reducing sugar, anthracenosides and digitoxine were found in two extracts while tannins catechics, flavone and gitoxine were detected only in one extract. The dosage of phenolic compounds confirmed the richness of this fungus in total polyphenols, its moderate richness in proanthocyanidins, the lack of flavonoids in the aqueous extract and the moderate richness of the hydro-ethanolic and ethanolic extracts in flavonoids. Regarding the antioxidant activities, the results obtained for the DPPH trapping test showed that the different extracts had low to moderate antioxidant activity with antioxidant activity index (IAA) ranged 0.29 to 0.97 respectively in aqueous and ethanolic extracts. The presence of these mycochemical compounds along with the identified antioxidant activities shows that this M. africana have some pharmacological potential.

scholarly journals Chemical Fingerprint Analysis of Phenolics of Albizia chinensis Based on Ultra-Performance LC-Electrospray Ionization-Quadrupole Time-of-Flight Mass Spectrometry and Antioxidant Activity

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Abha Chaudhary ◽  
Pushpinder Kaur ◽  
Neeraj Kumar ◽  
Bikram Singh ◽  
Shiv Awasthi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Electrospray Ionization ◽  
Time Of Flight ◽  
Ethanolic Extract ◽  
Ultra Performance Liquid Chromatography ◽  
Reversed Phase ◽  
Flight Mass Spectrometry ◽  
Ethanolic Extracts ◽  
C Nmr

Albizia species have been shown to have anti-inflammatory and antiallergic properties. However, efficient analytical methods for identification of their active constituents are still lacking. Ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study the phenolic composition of the ethanolic extracts of different parts (flowers, leaves, pods and bark) of A. chinensis. In addition, the antioxidant activity of the ethanolic extracts was evaluated by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical cation scavenging methods. Four compounds were isolated from the ethanolic extract of the flowers and characterized by 1H and 13C NMR spectroscopy as quercetin-3- O-rhamnoside, quercetin, quercetin-3- O-arabinofuranoside, and myricetin-3- O-rhamnoside. Separation and quantification of the phenolics was accomplished using a reversed-phase BEH C18 column with the mobile phase of methanol–water (0.05% formic acid), and detection wavelengths of 360 and 254 nm.

Evaluation of Phytochemicals and Pharmacological Activities of Ben-Cha-Lo-Ka-Wi-Chian Remedy

2019 ◽  
Vol 891 ◽  
pp. 52-59
Author(s):  
Chanai Noysang ◽  
Teerarat Pummarin
Keyword(s):  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Pharmacological Activities ◽  
Ethanolic Extracts ◽  
Reaction Test

Benchalokawichian (BLW) remedy is a Thai traditional medicine that has been notified in the List of Medicine Products of the National List of Essential Drugs A.D. 2006 and has long been used as an antipyretic. The phytochemicals of the 70% and 95% ethanolic extracts of were studied by color reaction test and HPTLC analysis. The BLW remedy showed a highest amounts of 70% ethanolic extractives (ca. 3.99 ± 0.60% of dry material weight). The several ethanolic extracts showed similar qualitative phytochemicals. The major of phytochemicals identified in these extracts were alkaloids, flavonoids, tannins, and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analysed 70% ethanolic extract showed a highest antioxidant activity at IC50= 526.09 µg/ml and both extracts not showed tyrosinase inhibitory effect. The 95% ethanolic extract showed the greatest anti-propionibacterium acnes activity with inhibition zone of 10.10 ± 0.45 mm and minimum inhibitory concentration (MIC) was 5.05 mg/ml

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