Effect of the Extraction Process on the Biological Activity of Lyophilized Apricot Extracts Recovered from Apricot Pomace

Dina Cheaib; Nada El Darra; Hiba Rajha; Iman El-Ghazzawi; Richard Maroun; Nicolas Louka

doi:10.3390/antiox7010011

Optimal Extraction of Ocimum basilicum Essential Oil by Association of Ultrasound and Hydrodistillation and Its Potential as a Biopesticide Against a Major Stored Grains Pest

Molecules ◽

10.3390/molecules25122781 ◽

2020 ◽

Vol 25 (12) ◽

pp. 2781 ◽

Cited By ~ 4

Author(s):

Eridiane da Silva Moura ◽

Lêda Rita D’Antonino Faroni ◽

Fernanda Fernandes Heleno ◽

Alessandra Aparecida Zinato Rodrigues ◽

Lucas Henrique Figueiredo Prates ◽

...

Keyword(s):

Biological Activity ◽

Essential Oil ◽

Insect Pests ◽

Extraction Process ◽

Ocimum Basilicum ◽

Gas Chromatograph ◽

Drying Temperature ◽

Mass Selective Detector ◽

Potential Control ◽

Selective Detector

The essential oil of basil (Ocimum basilicum) has significant biological activity against insect pests and can be extracted through various techniques. This work aimed to optimize and validate the extraction process of the essential oil of O. basilicum submitted to different drying temperatures of the leaves and extracted by the combination of a Clevenger method and ultrasound. The biological activity of the extracted oil under different conditions was evaluated for potential control of Sitophilus zeamais. The extraction method was optimized according to the sonication time by ultrasound (0, 8, 19, 31 and 38 min) and hydrodistillation (20, 30, 45, 60 and 70 min) and drying temperature (20, 30, 45, 60 and 70 °C). The bioactivity of the essential oil was assessed against adults of S. zeamais and the effects of each variable and its interactions on the mortality of the insects were evaluated. The best yield of essential oil was obtained with the longest sonication and hydrodistillation times and the lowest drying temperature of leaves. Higher toxicity of the essential oil against S. zeamais was obtained by the use of ultrasound for its extraction. The identification and the relative percentage of the compounds of the essential oil were performed with a gas chromatograph coupled to a mass selective detector. The performance of the method was assessed by studying selectivity, linearity, limits of detection (LOD) and quantification (LOQ), precision and accuracy. The LOD and LOQ values for linalool were 2.19 and 6.62 µg mL−1 and for estragole 2.001 and 6.063 µg mL−1, respectively. The coefficients of determination (R2) were >0.99. The average recoveries ranged between 71 and 106%, with coefficient of variation ≤6.4%.

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Development of an extraction procedure and analysis of electrostatically stabilized silanates from aqueous solutions

Oceanological and Hydrobiological Studies ◽

10.1515/ohs-2020-0022 ◽

2020 ◽

Vol 49 (3) ◽

pp. 247-254

Author(s):

Joanna Jabłońska ◽

Mariusz Kluska ◽

Nikolai Erchak ◽

Stanisław Popiel

Keyword(s):

Biological Activity ◽

Aqueous Solutions ◽

Stationary Phases ◽

Extraction Procedure ◽

Extraction Process ◽

Capillary Isotachophoresis ◽

Optimum Time ◽

Derivatives Of ◽

Water Vegetation

AbstractThe analyzed organosilicon derivatives of electrostatically stabilized silanates belong to a group of pentacoordinated compounds. These derivatives are: 1-(N-morpholiniomethyl)spirobi(1-sila-2,5-dioxacyclopentan-3-on)at, 1-(N-morpholiniomethyl)spirobi(1-sila-2,5-dioxa-4-methylcyclopentan-3-on)at, 1-(N-morpholiniomethyl) spirobi(1-sila-2,5-dioxa-4-(i-propyl)cyclopentan-3-on)at.In the course of the work, the extraction process and optimization of conditions for separation and determination of a mixture of electrostatically stabilized silanates were carried out using capillary isotachophoresis. Proper leading electrolytes were elaborated developed and the terminating electrolyte: 4,4’-bis[(1-morpholiniomethyl)spirobi(1-sila-2,5-dioxacyclopentan-3-on)at] was proposed. The extraction process involved the use of three stationary phases: octadecyl, octyl and phenylpropyl. The highest recovery values, approx. 94%, were obtained on the phenylpropyl column. The optimum time of analysis by the isotachophoretic technique did not exceed 12 min. The developed method of separation and determination of electrostatically stabilized silanates expands the possibility of research on biological activity of this group of compounds in aqueous solutions and surface water vegetation.

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Phytochemistry and biological activity of Prosopis fracta

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2020.14.1.585 ◽

2020 ◽

Vol 14 (1) ◽

pp. 25-33

Author(s):

Batol I. Dheeb ◽

Sumayah S. Hashim ◽

Rawad Kh. Hameed ◽

Shilan W. Hasan

Keyword(s):

Biological Activity ◽

Extraction Process ◽

The Body ◽

Valuable Insight ◽

Loss Of Function ◽

Freeze Dried ◽

Medicinal Properties ◽

Syringe Filter ◽

Phytochemical Studies ◽

Insight Into

Background: Prosopis fracta is a valuable and useful medicinal plant for the purpose of extracting flavonoids and it is one of the most important plants with medicinal properties. Prosopis fracta is found in many southern regions of Iran. Objective: the aim of this review Phytochemistry and biological activity of Prosopis fracta. Material and Methods: Initially, the powder was degreased by hexane and then after completion of the extraction process, we obtained a rotary solvent removal and the rest of the extract was frozen for retention, then it stored at -23°C. The freeze-dried samples are dissolved in methanol and permanently filtered through a 0.35 syringe filter. Results: Prosopis fracta extracts using to treat cancer, which destroying activity via mitochondrial changes, as showed a good example for increased reactive oxygen species (ROS) levels, SIRT3 activity and cell death in HT-29 related to the intestines and rectum cancer cells. Conclusion: Antimicrobial and phytochemical studies provide valuable insight into understanding media. Sometimes the idea of the route responsible for the activity of these phytochemicals against oxidizing compounds that act as a source of harmful chemical to the body is removal from service of protein and loss of function.

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Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072332 ◽

1973 ◽

Vol 31 ◽

pp. 378-379

Author(s):

G. Kasnic ◽

S. E. Stewart ◽

C. Urbanski

Keyword(s):

Breast Cancer ◽

Biological Activity ◽

Human Breast Cancer ◽

Osteogenic Sarcoma ◽

Human Tumor ◽

Structural Similarity ◽

Cell Tumor ◽

Human Breast ◽

Human Tumor Cell ◽

Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

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Freeze-Etch Crystal-Like Structures in Membranous Glomerulosclerosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063883 ◽

1969 ◽

Vol 27 ◽

pp. 394-395

Author(s):

Burton B. Silver

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Sodium Hypochlorite ◽

Kidney Tissue ◽

Extraction Process ◽

Donor Kidney ◽

Unknown Substance ◽

Etched Surface ◽

Standard Fixation ◽

Diseased Kidney

Tissue from a non-functional kidney affected with chronic membranous glomerulosclerosis was removed at time of trnasplantation. Recipient kidney tissue and donor kidney tissue were simultaneously fixed for electron microscopy. Primary fixation was in phosphate buffered gluteraldehyde followed by infiltration in 20 and then 40% glycerol. The tissues were frozen in liquid Freon and finally in liquid nitrogen. Fracturing and replication of the etched surface was carried out in a Denton freeze-etch device. The etched surface was coated with platinum followed by carbon. These replicas were cleaned in a 50% solution of sodium hypochlorite and mounted on 400 mesh copper grids. They were examined in an Siemens Elmiskop IA. The pictures suggested that the diseased kidney had heavy deposits of an unknown substance which might account for its inoperative state at the time of surgery. Such deposits were not as apparent in light microscopy or in the standard fixation methods used for EM. This might have been due to some extraction process which removed such granular material in the dehydration steps.

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Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090270 ◽

1976 ◽

Vol 34 ◽

pp. 58-59

Author(s):

John L. Beggs ◽

John D. Waggener ◽

Wanda Miller

Keyword(s):

Spinal Cord ◽

Biological Activity ◽

Horseradish Peroxidase ◽

Transport Mechanism ◽

Vasogenic Edema ◽

Vesicular Transport ◽

Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

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