scholarly journals Biochemical Evaluation of the Effects of Hydroxyurea in Vitro on Red Blood Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1599
Author(s):  
Cristiane Oliveira Renó ◽  
Grazielle Aparecida Silva Maia ◽  
Leilismara Sousa Nogueira ◽  
Melina de Barros Pinheiro ◽  
Danyelle Romana Alves Rios ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Low Cost ◽  
Healthy Individuals ◽  
In Vitro Effects ◽  
Almost All

Hydroxyurea (HU) is a low-cost, low-toxicity drug that is often used in diseases, such as sickle cell anemia and different types of cancer. Its effects on the red blood cells (RBC) are still not fully understood. The in vitro effects of HU were evaluated on the biochemical parameters of the RBC from healthy individuals that were treated with 0.6 mM or 0.8 mM HU for 30 min and 1 h. After 30 min, there was a significant increase in almost all of the parameters analyzed in the two concentrations of HU, except for the pyruvate kinase (PK) activity. A treatment with 0.8 mM HU for 1 h resulted in a reduction of the levels of lipid peroxidation, Fe3+, and in the activities of some of the enzymes, such as glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), and PK. After the incubation for 1 h, the levels of H2O2, lipid peroxidation, reduced glutathione (GSH), enzymatic activity (hexokinase, G6PD, and superoxide dismutase (SOD) were reduced with the treatment of 0.8 mM HU when compared with 0.6 mM. The results have suggested that a treatment with HU at a concentration of 0.8 mM seemed to be more efficient in protecting against the free radicals, as well as in treating diseases, such as sickle cell anemia. HU appears to preferentially stimulate the pentose pathway over the glycolytic pathway. Although this study was carried out with the RBC from healthy individuals, the changes described in this study may help to elucidate the mechanisms of action of HU when administered for therapeutic purposes.

In vitro effects of low level yellow laser irradiation on human red blood cells

2016 ◽  
Author(s):  
Mustafa S. Al Musawi ◽  
M.S. Jaafar ◽  
B.T. Al-Gailani ◽  
Naser M. Ahmed ◽  
Fatanah M. Suhaimi
Keyword(s):  
Red Blood Cells ◽  
Laser Irradiation ◽  
Blood Cells ◽  
Low Level ◽  
Human Red Blood Cells ◽  
Yellow Laser ◽  
In Vitro Effects

scholarly journals Elastic property of sickle cell anemia and sickle cell trait red blood cells

2021 ◽  
Vol 26 (09) ◽  
Author(s):  
Endris Muhammed ◽  
James Cooper ◽  
Daniel Devito ◽  
Robert Mushi ◽  
Maria del Pilar Aguinaga ◽  
...  
Keyword(s):  
Elastic Property ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Sickle Cell Trait

scholarly journals The effect of the antisickling compoundGBT1118 on the permeability of red blood cells from patients with sickle cell anemia

2019 ◽  
Vol 7 (6) ◽  
pp. e14027 ◽  
Author(s):  
Halima Al Balushi ◽  
Kobina Dufu ◽  
David C. Rees ◽  
John N. Brewin ◽  
Anke Hannemann ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells

scholarly journals Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease

2014 ◽  
Vol 6 (1) ◽  
pp. e2014066 ◽  
Author(s):  
Marco Marziali ◽  
Antonella Isgrò ◽  
Pietro Sodani ◽  
Javid Gaziev ◽  
Daniela Fraboni ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Marrow Transplant ◽  
Mixed Chimerism ◽  
Radical Cure ◽  
Hematopoietic Stem ◽  
Hematopoietic Chimerism

Allogeneic cellular gene therapy through hematopoietic stem cell transplantation is the only radical cure for congenital hemoglobinopathies like thalassemia and sickle cell anemia. Persistent mixed hematopoietic chimerism (PMC) has been described in thalassemia and sickle cell anemia. Here, we describe the clinical course of a 6-year-old girl who had received bone marrow transplant for sickle cell anemia. After the transplant, the patient showed 36% donor hematopoietic stem cells in the bone marrow, whereas in the peripheral blood there was evidence of 80%  circulating donor red blood cells (RBC). The analysis of apoptosis at the Bone Marrow  level suggests that Fas might contribute to the cell death of host erythroid precursors. The increase in NK cells and the regulatory T cell population observed in this patient suggests that these cells might contribute to the condition of mixed chimerism.

scholarly journals Deep Learning Models for Classification of Red Blood Cells in Microscopy Images to Aid in Sickle Cell Anemia Diagnosis

Electronics ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 427 ◽  
Author(s):  
Laith Alzubaidi ◽  
Mohammed A. Fadhel ◽  
Omran Al-Shamma ◽  
Jinglan Zhang ◽  
Ye Duan
Keyword(s):  
Deep Learning ◽  
Red Blood Cells ◽  
Transfer Learning ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Learning Models ◽  
Sickle Cells ◽  
Microscopy Images

Sickle cell anemia, which is also called sickle cell disease (SCD), is a hematological disorder that causes occlusion in blood vessels, leading to hurtful episodes and even death. The key function of red blood cells (erythrocytes) is to supply all the parts of the human body with oxygen. Red blood cells (RBCs) form a crescent or sickle shape when sickle cell anemia affects them. This abnormal shape makes it difficult for sickle cells to move through the bloodstream, hence decreasing the oxygen flow. The precise classification of RBCs is the first step toward accurate diagnosis, which aids in evaluating the danger level of sickle cell anemia. The manual classification methods of erythrocytes require immense time, and it is possible that errors may be made throughout the classification stage. Traditional computer-aided techniques, which have been employed for erythrocyte classification, are based on handcrafted features techniques, and their performance relies on the selected features. They also are very sensitive to different sizes, colors, and complex shapes. However, microscopy images of erythrocytes are very complex in shape with different sizes. To this end, this research proposes lightweight deep learning models that classify the erythrocytes into three classes: circular (normal), elongated (sickle cells), and other blood content. These models are different in the number of layers and learnable filters. The available datasets of red blood cells with sickle cell disease are very small for training deep learning models. Therefore, addressing the lack of training data is the main aim of this paper. To tackle this issue and optimize the performance, the transfer learning technique is utilized. Transfer learning does not significantly affect performance on medical image tasks when the source domain is completely different from the target domain. In some cases, it can degrade the performance. Hence, we have applied the same domain transfer learning, unlike other methods that used the ImageNet dataset for transfer learning. To minimize the overfitting effect, we have utilized several data augmentation techniques. Our model obtained state-of-the-art performance and outperformed the latest methods by achieving an accuracy of 99.54% with our model and 99.98% with our model plus a multiclass SVM classifier on the erythrocytesIDB dataset and 98.87% on the collected dataset.

In vitro aging of red blood cells and lipid peroxidation

1987 ◽  
Vol 60 (1-3) ◽  
pp. 163-166 ◽  
Author(s):  
Hermann Einsele ◽  
Michael R. Clemens ◽  
Herbert Remmer
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Aging

scholarly journals Detection of short-chain carbonyl products of lipid peroxidation from malaria-parasite (Plasmodium vinckei)-infected red blood cells exposed to oxidative stress

1988 ◽  
Vol 249 (1) ◽  
pp. 63-68 ◽  
Author(s):  
G D Buffinton ◽  
N H Hunt ◽  
W B Cowden ◽  
I A Clark
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Radical Scavenger ◽  
Parasite Plasmodium ◽  
Infected Rbcs ◽  
Carbonyl Products ◽  
Plasmodium Vinckei

Reversed-phase h.p.l.c. was used to detect 2,4-dinitrophenylhydrazine-reactive carbonyl products, which excludes malonaldehyde, in malaria-parasite (Plasmodium vinckei)-infected murine red blood cells (RBCs). A number of alkanals, 4-hydroxyalk-2-enals and alka-2,4-dienals were tentatively identified by comparison with authentic standards. The formation of 4-hydroxynon-2-enal, deca-2,4-dienal and hexanal was greater in P. vinckei-infected RBCs than in their uninfected counterparts and was increased by the presence of t-butyl hydroperoxide. Several of these aldehydes have previously been shown to be toxic to various types of cells, including P. falciparum, in vitro. The iron chelator desferrioxamine and the free-radical scavenger butylated hydroxyanisole inhibited the formation of these aldehydes. These experiments demonstrate that products of lipid peroxidation other than malonaldehyde are formed during the exposure of malaria-infected RBCs in vitro to drugs that generate reactive oxygen species and have anti-parasitic activity. The formation of products of this type during the natural course of malaria infection may have implications for the mechanisms underlying intra-RBC parasite death and the tissue damage associated with the disease.

scholarly journals Nocturnal enuresis and K+ transport in red blood cells from patients with sickle cell anemia

Haematologica ◽  
2016 ◽  
Vol 101 (12) ◽  
pp. e469-e472 ◽  
Author(s):  
S. Tewari ◽  
D. C. Rees ◽  
A. Hannemann ◽  
O. T. Gbotosho ◽  
H. W. M. Al Balushi ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Nocturnal Enuresis ◽  
K Transport

scholarly journals Biomechanics and biorheology of red blood cells in sickle cell anemia

2017 ◽  
Vol 50 ◽  
pp. 34-41 ◽  
Author(s):  
Xuejin Li ◽  
Ming Dao ◽  
George Lykotrafitis ◽  
George Em Karniadakis
Keyword(s):  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells
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