scholarly journals Prevention of Teratogenesis in Pregnancies of Obese Rats by Vitamin E Supplementation

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1173
Author(s):  
Martin Alcala ◽  
Victoria E. Bolado ◽  
Isabel Sánchez-Vera ◽  
Sonia Clapés ◽  
Francisco Dasí ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Radical Scavenging ◽  
Embryonic Stem ◽  
Buthionine Sulfoximine ◽  
Neural Tube Closure ◽  
Synthesis Inhibitor ◽  
Neuronal Precursors ◽  
Underlying Mechanisms ◽  
Vitamin E Supplementation

Congenital malformations are a common adverse outcome in pregnancies complicated by pregestational obesity, although the underlying mechanisms are still unrevealed. Our aim was to study the effect of oxidative stress in obesity-induced teratogenesis. Wistar rats were fed a high-fat diet for 13 weeks, with (OE group) or without (O group) vitamin E supplementation. Then, rats were mated and sacrificed at day 11.5 of gestation. Embryos from O dams presented a 25.9 ± 3.5% rate of malformations (vs. 8.7 ± 3.4% in C rats), which was reduced in the OE group (11.5 ± 2.3%). Pregestational obesity induced hepatic protein and DNA oxidation and a decline in antioxidant enzymes. Importantly, glutathione content was also decreased, limiting the availability of this antioxidant in the embryos. Vitamin E supplementation efficiently maintained glutathione levels in the obese mothers, which could be used in their embryos to prevent oxidation-induced malformations. To test the effect of decreasing glutathione levels alone in a cell culture model of neuroepithelium, murine embryonic stem cells (ESC) were induced to form neuronal precursors and glutathione synthesis was inhibited with the gamma–glutamylcysteine synthesis inhibitor, buthionine sulfoximine (BSO). BSO inhibited the expression of Pax3, a gene required for neural tube closure that is also inhibited by oxidative stress. Taken together, our data indicate that obesity causes malformations through the depletion of maternal glutathione, thereby decreasing glutathione-dependent free radical scavenging in embryos, which can be prevented by vitamin E supplementation.

Vitamin E Supplementation Increases LDL Resistance to ex vivo Oxidation in Hemodialysis Patients

2003 ◽  
Vol 73 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Badiou ◽  
Cristol ◽  
Morena ◽  
Bosc ◽  
Carbonneau ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
High Performance ◽  
Ex Vivo ◽  
Density Lipoprotein ◽  
Thiobarbituric Acid ◽  
Hemodialysis Patients ◽  
Ldl Oxidation ◽  
Oral Vitamin ◽  
Vitamin E Supplementation

Background: Oxidative stress and alterations in lipid metabolism observed in hemodialysis patients potentiate the low-density lipoprotein (LDL) oxidability, recognized as a key event during early atherogenesis. Objective: To explore the effects of an oral vitamin E supplementation on oxidative stress markers and LDL oxidability in hemodialysis patients. Methods: Fourteen hemodialysis patients and six healthy volunteers were given oral vitamin E (500 mg/day) for six months. Oxidative stress was assessed using: plasma and lipoprotein vitamin E levels [high-performance liquid chromatography (HPLC) procedure]; thiobarbituric acid reactive substances (TBARS, Yaggi method); and copper-induced LDL oxidation. All parameters were evaluated before initiation of vitamin E supplementation, and at three and six months thereafter. Results: At baseline, a significantly higher TBARS concentration and a higher LDL oxidability were observed in hemodialysis patients when compared to controls. After six months of vitamin E supplementation, TBARS and LDL oxidability were normalized in hemodialysis patients. Conclusion: Our data confirm that hemodialysis patients are exposed to oxidative stress and increased susceptibility to ex vivo LDL oxidation. Since oral vitamin E supplementation prevents oxidative stress and significantly increases LDL resistance to ex vivo oxidation, supplementation by natural antioxidants such as vitamin E may be beneficial in hemodialysis patients.

scholarly journals The effects of vitamin E supplementation on autoimmune-prone New Zealand black × New Zealand white F1 mice fed an oxidised oil diet

2005 ◽  
Vol 93 (5) ◽  
pp. 655-662 ◽  
Author(s):  
Chia-Chien Hsieh ◽  
Bi-Fong Lin
Keyword(s):  
Oxidative Stress ◽  
New Zealand ◽  
Vitamin E ◽  
Autoimmune Disease ◽  
Mhc Class Ii ◽  
Tocopheryl Acetate ◽  
Thiobarbituric Acid Reactive Substance ◽  
Igg Antibody ◽  
Double Stranded Dna ◽  
Vitamin E Supplementation

The purpose of the present study was to investigate the effect of vitamin E supplementation on autoimmune disease in New Zealand black×New Zealand white F1(NZB/W F1) female mice fed an oxidised oil diet. First, 5-month-old mice were fed an AIN-76 diet containing either 150 g fresh soyabean oil/kg (15S), 50 g fresh soyabean oil/kg + 100 g oxidised frying oil/kg (5S10F) or 5S10F supplemented with all-rac-α-tocopheryl acetate at 275 mg/kg diet level (5S10F5E) or 550 mg/kg (5S10F10E), respectively, in experiment 1. The results showed that mice fed the 5S10F10E diet had a lower anti-double-stranded DNA IgG antibody level and a longer lifespan than those fed the 15S and 5S10F diets. Therefore, the 5S10F and 5S10F10E treatments were repeated in experiment 2 for further analysis. The results showed that vitamin E supplementation in the oxidised oil significantly decreased thiobarbituric acid-reactive substance values in the kidney and spleen of NZB/W F1mice. Interferon-γ and IL-6 production by mitogen-stimulated splenocytes decreased in mice fed the 5S10F10E diet, whereas the secretion of IL-2 and IL-10 was not affected. The percentage of T-cells was significantly higher and that of MHC class II-bearing cells was lower in the spleens of the 5S10F10E group. The 5S10F10E group had a significantly higher linoleic acid (18: 2n-6) composition than the 5S10F diet group. Therefore, vitamin E supplementation in oxidised oil might decrease oxidative stress, anti-double-stranded DNA IgG antibody, regulate cytokines and lymphocyte subsets, and subsequently alleviate the severity of autoimmune disease such as systemic lupus erythematosus under oxidative stress.

Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients

Metabolism ◽  
2000 ◽  
Vol 49 (2) ◽  
pp. 160-162 ◽  
Author(s):  
Anjali Sharma ◽  
Simmi Kharb ◽  
S.N. Chugh ◽  
Rajesh Kakkar ◽  
G.P. Singh
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Diabetic Patients ◽  
Before And After ◽  
Vitamin E Supplementation

Feasibility of Using Vitamin E-Loaded Poly(ε-caprolactone)/Gelatin Nanofibrous Mat to Prevent Oxidative Stress in Skin

2020 ◽  
Vol 20 (6) ◽  
pp. 3554-3562 ◽  
Author(s):  
Saba Kalantary ◽  
Farideh Golbabaei ◽  
Masoud Latifi ◽  
Mohammad Ali Shokrgozar ◽  
Mehdi Yaseri
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Electrical Potential ◽  
Radical Scavenging ◽  
Occupational Exposures ◽  
Skin Cells ◽  
Novel Approach ◽  
Tert Butyl Hydroperoxide ◽  
Increased Risk

Some occupational skin exposures lead to the formation of reactive oxygen species (ROS). The occupational exposure of workers to ROS has been found to be associated with an increased risk of developing skin injuries; therefore, it is essential to protect skin against ROS formation. Recently, some studies have been conducted on introducing better alternatives for skin protection. Nanofibers are good candidates for this purpose. The current study was carried out to assess vitamin E-loaded hybrid Poly(ε-caprolactone) (PCL)/gelatin (Gt) nanofibres mats as protective layers of skin exposed to occupational exposures. Vitamin E (VE) was successfully incorporated into PCL/Gt nanofibers while they were formed by electrospinning method. Nanofibers mats were characterized using scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR). Their degradation behavior, in vitro release, biocompatibility, and antioxidant activity were studied. The diameters of the PCL/Gt/VE nanofibers decreased with the addition of vitamin E. The degradation rate of nanofibers was equal to 42.98 and 50.69% during 7 and 14 days, respectively. Nanofibers containing vitamin E showed an initial burst followed by a sustained release. The PCL/Gt/VE nanofibers exhibited good free radical scavenging activities despite being exposed to a high electrical potential during electrospinning. PCL/Gt/VE nanofibers supported a higher level of viability compared to PCL/Gt ones and significantly assisted human skin cells against tert-Butyl hydroperoxide (t-BHP) induced oxidative stress. Overall, PCL/Gt/VE nanofibers can potentially be used to protect skin against oxidative stress as a novel approach for worker’s healthcare.

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