scholarly journals Recovery of Polyphenols from Vineyard Pruning Wastes—Shoots and Cane of Hybrid Grapevine (Vitis sp.) Cultivars

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1059
Author(s):  
Reelika Rätsep ◽  
Kadri Karp ◽  
Mariana Maante-Kuljus ◽  
Alar Aluvee ◽  
Hedi Kaldmäe ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Environmentally Friendly ◽  
Polyphenolic Compounds ◽  
Biomass Valorization ◽  
Qualitative And Quantitative ◽  
Winter Conditions ◽  
Quantitative Analyses ◽  
Vitis Sp

Grapevine shoots and canes represent a significant amount of biomass, considered as a waste in viticulture. In cooler climates, grapevines are pruned in the autumn (October) and spring (March) due to harsh winter conditions (e.g., snow, low temperatures), and large amounts of biomass are produced at these different pruning times. This work was undertaken in order to investigate the potential of vineyard pruning waste for recovery of polyphenolic compounds for biomass valorization. Qualitative and quantitative analyses of grapevine shoot and cane polyphenols, including flavonoids and stilbenoids were performed using UHPLC MS/MS method. The results revealed the flavonols (quercetin) to be the most abundant compounds in shoots among all the three cultivars screened (Zilga, Hasansky Sladky, Rondo). Stilbenoids (ε-viniferin) dominated in the canes, while increased level of flavonols with lower contents of stilbenoids was detected in the endo-dormant canes, and higher amounts of flavanols and stilbenoids were recorded in eco-dormant canes. In conclusion, the content of polyphenols in grapevine shoots and canes differed among the cultivars and dormancy phases. The results generated from the present study contribute to the sustainable and environmentally friendly viticulture practice via valorization of vineyard pruning wastes.

scholarly journals Determination of chlorogenic and gallic acids by UPLC-MS/MS

2013 ◽  
Vol 59 (1) ◽  
pp. 7-16
Author(s):  
Agnieszka Gryszczyńska ◽  
Bogna Opala ◽  
Zdzisław Łowicki ◽  
Anna Krajewska-Patan ◽  
Waldemar Buchwald ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Chromatographic Method ◽  
Ultra Performance Liquid Chromatography ◽  
Polyphenolic Compounds ◽  
Qualitative And Quantitative ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Quantitative Analyses ◽  
Polyphenolic Acids

Summary The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.

In situ microprobe quantitation of inorganic particle burden in paraffin sections of lung tissue

1988 ◽  
Vol 46 ◽  
pp. 990-991
Author(s):  
Jerrold L. Abraham
Keyword(s):  
Lung Tissue ◽  
Quantitative Information ◽  
Particulate Material ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Qualitative And Quantitative ◽  
The Past ◽  
Quantitative Analyses ◽  
Data Result

Inorganic particulate material of diverse types is present in the ambient and occupational environment, and exposure to such materials is a well recognized cause of some lung disease. To investigate the interaction of inhaled inorganic particulates with the lung it is necessary to obtain quantitative information on the particulate burden of lung tissue in a wide variety of situations. The vast majority of diagnostic and experimental tissue samples (biopsies and autopsies) are fixed with formaldehyde solutions, dehydrated with organic solvents and embedded in paraffin wax. Over the past 16 years, I have attempted to obtain maximal analytical use of such tissue with minimal preparative steps. Unique diagnostic and research data result from both qualitative and quantitative analyses of sections. Most of the data has been related to inhaled inorganic particulates in lungs, but the basic methods are applicable to any tissues. The preparations are primarily designed for SEM use, but they are stable for storage and transport to other laboratories and several other instruments (e.g., for SIMS techniques).

Qualitative and quantitative analyses of some Plantago species from Serbia and Montenegro

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Jankovic ◽  
G Zdunic ◽  
K Savikin ◽  
I Beara ◽  
N Mimica-Dukić
Keyword(s):  
Serbia And Montenegro ◽  
Qualitative And Quantitative ◽  
Quantitative Analyses

scholarly journals Assessment of Photo-Induced Cytotoxic Activity of Cachrys sicula and Cachrys libanotis Enriched-Coumarin Extracts against Human Melanoma Cells

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 123
Author(s):  
Mariangela Marrelli ◽  
Maria Rosaria Perri ◽  
Valentina Amodeo ◽  
Francesca Giordano ◽  
Giancarlo A. Statti ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Melanoma Cells ◽  
Therapeutic Approaches ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Naturally Occurring ◽  
Quantitative Analyses ◽  
Solid Liquid ◽  
Uva Radiation

Photochemotherapy is one of the most interesting current therapeutic approaches for the treatment of melanoma. Different classes of naturally occurring phytochemicals demonstrated interesting photoactive properties. The aim of this study was to evaluate the photocytotoxic potential of two Cachrys species from Southern Italy: C. sicula and C. libanotis (Apiaceae). The enriched-coumarin extracts were obtained from aerial parts through both traditional maceration and pressurized cyclic solid-liquid (PCSL) extraction using Naviglio extractor®. Qualitative and quantitative analyses of furanocoumarins were performed with GC-MS. The photocytotoxic effects were verified on C32 melanoma cells irradiated at a dose of 1.08 J/cm2. The apoptotic responses were also assessed. Moreover, phenolic content and the in vitro antioxidant potential were estimated. Xanthotoxin, bergapten, and isopimpinellin were identified. All the samples induced concentration-dependent photocytotoxic effects (IC50 ranging from 3.16 to 18.18 μg/mL). The C. libanotis sample obtained with Naviglio extractor® was the most effective one (IC50 = 3.16 ± 0.21 μg/mL), followed by C. sicula sample obtained with the same technique (IC50 = 8.83 ± 0.20 μg/mL). Both Cachrys samples obtained through PCSL induced up-regulation of apoptotic signals such as BAX (Bcl2-associated X protein) and PARP (poly ADP-ribose polymerase) cleavage. Moreover, these samples proved to be more photoactive, giving a greater upregulation of p21 protein in the presence of UVA radiation. Obtained results suggest that investigated species could be promising candidates for further investigations aimed to find new potential drugs for the photochemotherapy of skin cancer.

scholarly journals A framework for identifying and learning from countries that demonstrated exemplary performance in improving health outcomes and systems

2020 ◽  
Vol 5 (12) ◽  
pp. e002938
Author(s):  
Austin Carter ◽  
Nadia Akseer ◽  
Kevin Ho ◽  
Oliver Rothschild ◽  
Niranjan Bose ◽  
...  
Keyword(s):  
Mixed Methods ◽  
Global Health ◽  
Health Outcomes ◽  
Mixed Methods Research ◽  
Multidisciplinary Teams ◽  
Ongoing Research ◽  
Qualitative And Quantitative ◽  
Success Stories ◽  
Quantitative Analyses ◽  
Comprehensive Framework

This paper introduces a framework for conducting and disseminating mixed methods research on positive outlier countries that successfully improved their health outcomes and systems. We provide guidance on identifying exemplar countries, assembling multidisciplinary teams, collecting and synthesising pre-existing evidence, undertaking qualitative and quantitative analyses, and preparing dissemination products for various target audiences. Through a range of ongoing research studies, we illustrate application of each step of the framework while highlighting key considerations and lessons learnt. We hope uptake of this comprehensive framework by diverse stakeholders will increase the availability and utilisation of rigorous and comparable insights from global health success stories.

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