scholarly journals Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1025
Author(s):  
Roberto Gualtieri ◽  
Guruprasad Kalthur ◽  
Vincenza Barbato ◽  
Salvatore Longobardi ◽  
Francesca Di Rella ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Embryo Development ◽  
Cell Damage ◽  
Sperm Function ◽  
Ros Production ◽  
Vitro Fertilization ◽  
Reproductive Techniques ◽  
Strand Breakage ◽  
Dna Strand

Reactive oxygen species (ROS) generated at low levels during mitochondrial respiration have key roles in several signaling pathways. Oxidative stress (OS) arises when the generation of ROS exceeds the cell’s antioxidant scavenging ability and leads to cell damage. Physiological ROS production in spermatozoa regulates essential functional characteristics such as motility, capacitation, acrosome reaction, hyperactivation, and sperm-oocyte fusion. OS can have detrimental effects on sperm function through lipid peroxidation, protein damage, and DNA strand breakage, which can eventually affect the fertility of an individual. Substantial evidence in the literature indicates that spermatozoa experiencing OS during in vitro manipulation procedures in human- and animal-assisted reproduction are increasingly associated with iatrogenic ROS production and eventual impairment of sperm function. Although a direct association between sperm OS and human assisted reproductive techniques (ART) outcomes after in vitro fertilization (IVF) and/or intracytoplasmic sperm injection (ICSI) is still a matter of debate, studies in animal models provide enough evidence on the adverse effects of sperm OS in vitro and defective fertilization and embryo development. This review summarized the literature on sperm OS in vitro, its effects on functional ability and embryo development, and the approaches that have been proposed to reduce iatrogenic sperm damage and altered embryonic development.

scholarly journals A New Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures

2018 ◽  
Author(s):  
Matteo Becatti ◽  
Rossella Fucci ◽  
Amanda Mannucci ◽  
Victoria Barygina ◽  
Marco Mugnaini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cells ◽  
Redox Status ◽  
Blood Leukocytes ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Stress Markers ◽  
Reproductive Techniques ◽  
Biochemical Approach

Oxidative stress negatively affects folliculogenesis and embryo development. However, a reliable and biologically accurate indicator of oxidative stress does not yet exist. On these bases, the aim of this study was to assess -and compare- blood and follicular fluid (FF) redox status in 45 infertile subjects (and 45 age-matched controls) undergoing in vitro fertilization (IVF) and to establish its connection with the outcome of IVF. Blood and FF were obtained at the time of egg retrieval and immediately analyzed. Firstly, ROS production in blood leukocytes and in granulosa cells was assessed. Oxidative stress markers in blood and in granulosa cells resulted significantly (p<0.001) increased in infertile patients compared to controls. Then, a redox index was obtained in plasma and in FF of patients and controls. The main findings emerging from our study in infertile women are: i) blood oxidative stress reflects FF oxidative stress as demonstrated by the significant correlation between blood redox markers and FF redox markers; ii) a significant correlation between oxidative stress parameters and the considered IVF outcomes is present. We suggest the strict monitoring of the redox parameters for the improvement of assisted reproductive techniques success rate and infertility management.

226 CRYOPRESERVED EJACULATED AND EPIDIDYMAL SPERM COLLECTED FROM THE SAME HOLSTEIN BULLS USED FOR IN VITRO FERTILIZATION

2013 ◽  
Vol 25 (1) ◽  
pp. 261 ◽  
Author(s):  
M. A. Stout ◽  
J. R. Saenz ◽  
J. F. Chenevert ◽  
G. T. Gentry ◽  
K. B. Bondioli ◽  
...  
Keyword(s):  
Embryo Development ◽  
Epididymal Sperm ◽  
In Vitro Production ◽  
Blastocyst Development ◽  
Vitro Fertilization ◽  
Mean Values ◽  
Treatment Groups ◽  
Reproductive Techniques ◽  
Holstein Bulls

Exposure to seminal plasma may modify the ability of sperm to survive cryopreservation, undergo capacitation, and fertilize oocytes. The present work was designed to compare embryo development after IVF of oocytes with ejaculated and epididymal bovine sperm from bulls previously tested and showing similar responses to freezing. However, we also found that this ejaculated and epididymal sperm differed in their in vitro culture dynamics (capacitation, viability, and auto-acrosome reaction) and ability to fertilize oocytes in vitro. Ejaculated and epididymal sperm were collected from the same fertile mature Holstein bulls (n = 4) by artificial vagina and post-castration retrograde caudal epididymal flush, respectively. Collection of epididymal sperm was conducted 2 weeks after the last collection of ejaculated sperm. After collection, ejaculated and epididymal sperm were cryopreserved and stored in LN until use. Before IVF, a viable sperm population was isolated by centrifugation through a Percoll density gradient. Ejaculated and epididymal sperm were then added to fertilization drops at a final concentration of 1 × 106 mL–1, and IVF was conducted with and without the capacitation agent heparin. Oocytes were washed and randomly assigned to one of four treatment groups (ejaculated ± heparin or epididymal ± heparin). Embryo development was determined at 72 and 186 h after IVF. Differences in the mean values among treatment groups were analysed by one-way ANOVA, followed by the Holm-Sidak pairwise multiple comparisons. Embryo cleavage after IVF using ejaculated sperm without heparin (45.2%) was significantly lower (P < 0.05) than in all other groups. Cleavage rates of ejaculated sperm with heparin (56.9%) and epididymal sperm with (58.1%) and without (57.5%) heparin were found to be similar. No difference was noted between ejaculated and epididymal sperm in blastocyst development, although the inclusion of heparin did significantly (P < 0.01) increase blastocyst development in both ejaculated (9.3 compared with 23.6%) and epididymal sperm (2.5 compared with 23.3%). In conclusion, cryopreserved ejaculated and epididymal sperm collected from the same bulls can be successfully used for the in vitro production of bovine blastocysts without changing the existing protocols. This may increase the efficiency when using epididymal sperm in assisted reproductive techniques.

scholarly journals Mechanisms of Mitochondrial ROS Production in Assisted Reproduction: The Known, the Unknown, and the Intriguing

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 933 ◽  
Author(s):  
James N. Cobley
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Assisted Reproduction ◽  
Redox Signaling ◽  
Ros Production ◽  
Vitro Fertilization ◽  
Assisted Reproduction Technologies ◽  
Mitochondrial Ros ◽  
Species Specific

The consensus that assisted reproduction technologies (ART), like in vitro fertilization, to induce oxidative stress (i.e., the known) belies how oocyte/zygote mitochondria—a major presumptive oxidative stressor—produce reactive oxygen species (ROS) with ART being unknown. Unravelling how oocyte/zygote mitochondria produce ROS is important for disambiguating the molecular basis of ART-induced oxidative stress and, therefore, to rationally target it (e.g., using site-specific mitochondria-targeted antioxidants). I review the known mechanisms of ROS production in somatic mitochondria to critique how oocyte/zygote mitochondria may produce ROS (i.e., the unknown). Several plausible site- and mode-defined mitochondrial ROS production mechanisms in ART are proposed. For example, complex I catalyzed reverse electron transfer-mediated ROS production is conceivable when oocytes are initially extracted due to at least a 10% increase in molecular dioxygen exposure (i.e., the intriguing). To address the term oxidative stress being used without recourse to the underlying chemistry, I use the species-specific spectrum of biologically feasible reactions to define plausible oxidative stress mechanisms in ART. Intriguingly, mitochondrial ROS-derived redox signals could regulate embryonic development (i.e., their production could be beneficial). Their potential beneficial role raises the clinical challenge of attenuating oxidative damage while simultaneously preserving redox signaling. This discourse sets the stage to unravel how mitochondria produce ROS in ART, and their biological roles from oxidative damage to redox signaling.

scholarly journals Alternative polyadenylation trans-factor FIP1 exacerbates UUO/IRI-induced kidney injury and contributes to AKI-CKD transition via ROS-NLRP3 axis

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Tong Zheng ◽  
Yuqin Tan ◽  
Jiang Qiu ◽  
Zhenwei Xie ◽  
Xiao Hu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Therapeutic Potential ◽  
Cell Damage ◽  
Kidney Injury ◽  
Alternative Polyadenylation ◽  
Decisive Role ◽  
Ros Production ◽  
Rich Domain

AbstractNLRP3, a decisive role in inflammation regulation, is obviously upregulated by oxidative stress in kidney injury. The NLRP3 upregulation leads to unsolved inflammation and other pathological effects, contributing to aggravation of kidney injury and even transition to chronic kidney disease (CKD). However, the mechanism for NLRP3 upregulation and further aggravation of kidney injury remains largely elusive. In this study, we found NLRP3 3′UTR was shortened in response to kidney injury in vivo and oxidative stress in vitro. Functionally, such NLRP3 3′UTR shortening upregulated NLRP3 expression and amplified inflammation, fibrogenesis, ROS production and apoptosis, depending on stabilizing NLRP3 mRNA. Mechanistically, FIP1 was found to bind to pPAS of NLRP3 mRNA via its arginine-rich domain and to induce NLRP3 3′UTR shortening. In addition, FIP1 was upregulated in CKD specimens and negatively associated with renal function of CKD patients. More importantly, we found FIP1 was upregulated by oxidative stress and required for oxidative stress-induced NLRP3 upregulation, inflammation activation, cell damage and apoptosis. Finally, we proved that FIP1 silencing attenuated the inflammation activation, fibrogenesis, ROS production and apoptosis induced by UUO or IRI. Taken together, our results demonstrated that oxidative stress-upregulated FIP1 amplified inflammation, fibrogenesis, ROS production and apoptosis via inducing 3′UTR shortening of NLRP3, highlighting the importance of crosstalk between oxidative stress and alternative polyadenylation in AKI-CKD transition, as well as the therapeutic potential of FIP1 in kidney injury treatment.

scholarly journals Vitamin E-Coated Dialyzer Inhibits Oxidative Stress

2017 ◽  
Vol 44 (4) ◽  
pp. 288-293 ◽  
Author(s):  
Shiho Yamadera ◽  
Yuya Nakamura ◽  
Masahiro Inagaki ◽  
Isao Ohsawa ◽  
Hiromichi Gotoh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Synthetic Polymer ◽  
Intracellular Reactive Oxygen Species ◽  
Ros Production ◽  
Oxygen Species ◽  
In Vitro Methods ◽  
Stress Effects ◽  
Intracellular Ros

Aim: To examine the effects of vitamin E-coated dialyzer on oxidative stress in vitro. Methods: A dialyzer with a synthetic polymer membrane (APS-11SA) and vitamin E-coated dialyzer (VPS-11SA) were connected to a blood tubing line, and U937 cells were circulated in the device. The circulating fluid was collected at 1, 2, 5, 10, 25, and 50 cycles, which are estimated numbers of passes through the dialyzer. Intracellular reactive oxygen species (ROS) production, malondialdehyde (MDA), and Cu/Zn-superoxide dismutase (SOD) were quantified. Results: Intracellular ROS production was increased in the first cycle by APS-11SA and was decreased throughout the experiment by VPS-11SA. Intracellular ROS production in the VPS-11SA device was lower, and MDA levels were decreased. MDA levels were lower during VPS-11SA processing than during APS-11SA processing. Cu/Zn-SOD levels remained unchanged. Conclusion: Our results highlight anti-oxidative-stress effects of a vitamin E-coated dialyzer.

Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

2020 ◽  
Vol 55 (9) ◽  
pp. 1103-1114
Author(s):  
Pei‐Pei Zhang ◽  
Jing‐Jing Wang ◽  
Chong‐Yang Li ◽  
Hai‐sheng Hao ◽  
Hao‐Yu Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Vitamin C ◽  
Protective Effect ◽  
Vitro Fertilization ◽  
Bull Sperm ◽  
Fertilization Capacity
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