scholarly journals Mechanistic Insight into the Regulation of Lipoxygenase-Driven Lipid Peroxidation Events in Human Spermatozoa and Their Impact on Male Fertility

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 43
Author(s):  
Jessica L. H. Walters ◽  
Amanda L. Anderson ◽  
Sarah J. Martins da Silva ◽  
R. John Aitken ◽  
Geoffry N. De Iuliis ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Male Infertility ◽  
Human Sperm ◽  
Protective Role ◽  
Membrane Lipid ◽  
Human Spermatozoa ◽  
Reactive Carbonyl Species ◽  
Sperm Membrane ◽  
Infertility Patients ◽  
Cellular Dysfunction

A prevalent cause of sperm dysfunction in male infertility patients is the overproduction of reactive oxygen species, an attendant increase in lipid peroxidation and the production of cytotoxic reactive carbonyl species such as 4-hydroxynonenal. Our previous studies have implicated arachidonate 15-lipoxygenase (ALOX15) in the production of 4-hydroxynonenal in developing germ cells. Here, we have aimed to develop a further mechanistic understanding of the lipoxygenase-lipid peroxidation pathway in human spermatozoa. Through pharmacological inhibition studies, we identified a protective role for phospholipase enzymes in the liberation of peroxidised polyunsaturated fatty acids from the human sperm membrane. Our results also revealed that arachidonic acid, linoleic acid and docosahexanoic acid are key polyunsaturated fatty acid substrates for ALOX15. Upon examination of ALOX15 in the spermatozoa of infertile patients compared to their normozoospermic counterparts, we observed significantly elevated levels of ALOX15 protein abundance in the infertile population and an increase in 4-hydroxynonenal adducts. Collectively, these data confirm the involvement of ALOX15 in the oxidative stress cascade of human spermatozoa and support the notion that increased ALOX15 abundance in sperm cells may accentuate membrane lipid peroxidation and cellular dysfunction, ultimately contributing to male infertility.

Effect of storage for 24 h at 18°C on sperm quality and a comparison of two assays for sperm membrane lipid peroxidation

2010 ◽  
Vol 90 (3) ◽  
pp. 389-392 ◽  
Author(s):  
N. Am-in ◽  
R N Kirkwood ◽  
M. Techakumphu ◽  
W. Tantasuparuk
Keyword(s):  
Lipid Peroxidation ◽  
Membrane Permeability ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation ◽  
Group A ◽  
Sperm Membrane ◽  
Fresh Semen

Boars having normal (71.1 ± 1.2%; n = 10) or low (35.12 &plusmn 3.9%; n = 10) sperm motility 24 h after collection were used, and semen was evaluated following storage in Beltsville Thawing Solution (BTS) for 24 h at 18°C. Sperm lipids were extracted and lipid peroxidation quantified. No differences were evident in fresh semen, but after 24 h, sperm motility, viability and membrane permeability in the low motility group were lower (P < 0.001) compared with the normal motility group. Sperm membrane lipid peroxidation was greater (P < 0.001) in the low motility group. A factor influencing sperm storability is membrane lipid peroxidation, which can be accurately assayed using a commercial kit.Key words: Boars, sperm motility, sperm quality, lipid peroxidation

Cytotoxic effects of membrane lipid peroxidation products on human spermatozoa

2015 ◽  
Vol 104 (3) ◽  
pp. e146 ◽  
Author(s):  
A.M. Polhemus ◽  
R. Moazamian ◽  
H. Connaughton ◽  
B. Fraser ◽  
S. Whiting ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Membrane Lipid ◽  
Human Spermatozoa ◽  
Membrane Lipid Peroxidation ◽  
Cytotoxic Effects ◽  
Lipid Peroxidation Products

Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation

2014 ◽  
Vol 102 (3) ◽  
pp. 705-710 ◽  
Author(s):  
Paul B. Marshburn ◽  
Allie Giddings ◽  
Stephanie Causby ◽  
Michelle L. Matthews ◽  
Rebecca S. Usadi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation ◽  
Total Antioxidant ◽  
Sperm Membrane ◽  
Ejaculatory Abstinence

scholarly journals Extracellular domain of YWK-II, a human sperm transmembrane protein, interacts with rat Mullerian-inhibiting substance

Reproduction ◽  
2001 ◽  
pp. 873-880 ◽  
Author(s):  
XY Tian ◽  
YS Sha ◽  
SM Zhang ◽  
YB Chen ◽  
SY Miao ◽  
...  
Keyword(s):  
Hybrid System ◽  
Transmembrane Protein ◽  
Human Sperm ◽  
Extracellular Domain ◽  
Human Spermatozoa ◽  
Müllerian Inhibiting Substance ◽  
Sperm Membrane ◽  
Two Hybrid ◽  
Mullerian Inhibiting Substance

The YWK-II protein in human spermatozoa is structurally related to the betaA4-amyloid precursor protein of Alzheimer disease and has high similarity with amyloid precusor homologues. Antibodies to the YWK-II protein agglutinate human spermatozoa and may be a potential cause of infertility. In the present study, a yeast two-hybrid system (MATCHMAKER Two-Hybrid System 2; Clontech, Palo Alto, CA) was used to screen a rat ovary cDNA library for potential ligands capable of interacting with the YWK-II component. Mullerian-inhibiting substance was found to interact with the extracellular domain of YWK-II protein. The interaction was confirmed by a binding experiment in vitro and surface plasmon resonance assays. The recombinant Mullerian-inhibiting substance can significantly increase the viability and longevity of human spermatozoa after 5 and 22 h of incubation, presumably through binding the YWK-II component on the sperm membrane. The results of this study indicate that the YWK-II sperm membrane protein may function as a receptor for Mullerian-inhibiting substance.

scholarly journals Exogenous Aspergillus aculeatus Enhances Drought and Heat Tolerance of Perennial Ryegrass

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaoning Li ◽  
Chuncheng Zhao ◽  
Ting Zhang ◽  
Guangyang Wang ◽  
Erick Amombo ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Heat Stress ◽  
Perennial Ryegrass ◽  
Protective Role ◽  
Membrane Lipid ◽  
Photosynthetic Performance ◽  
Expression Level ◽  
Induced Response ◽  
Aspergillus Aculeatus ◽  
Drought And Heat Stress

Perennial ryegrass (Lolium perenne) is a cool-season grass whose growth and development are limited by drought and high temperature. Aspergillus aculeatus has been reported to promote plant growth and counteract the adverse effects of abiotic stresses. The objective of this study was to assess A. aculeatus-induced response mechanisms to drought and heat resistance in perennial ryegrass. We evaluated the physiological and biochemical markers of drought and heat stress based on the hormone homeostasis, photosynthesis, antioxidant enzymes activity, lipid peroxidation, and genes expression level. We found out that under drought and heat stress, A. aculeatus-inoculated leaves exhibited higher abscisic acid (ABA) and lower salicylic acid (SA) contents than non-inoculated regimes. In addition, under drought and heat stress, the fungus enhanced the photosynthetic performance, decreased the antioxidase activities, and mitigated membrane lipid peroxidation compared to non-inoculated regime. Furthermore, under drought stress, A. aculeatus induced a dramatic upregulation of sHSP17.8 and DREB1A and a downregulation of POD47, Cu/ZnSOD, and FeSOD genes. In addition, under heat stress, A. aculeatus-inoculated plants exhibited a higher expression level of HSP26.7a, sHSP17.8, and DREB1A while a lower expression level of POD47 and FeSOD than non-inoculated ones. Our results provide an evidence of the protective role of A. aculeatus in perennial ryegrass response to drought and heat stresses.

307 EFFECT OF BULL EPIDIDYMIS STORAGE CONDITIONS ON SPERM RESISTANCE AGAINST LIPID PEROXIDATION AND SUBSEQUENT IN VITRO EMBRYO PRODUCTION

2006 ◽  
Vol 18 (2) ◽  
pp. 261 ◽  
Author(s):  
M. Nichi ◽  
J. B. P. De Clercq ◽  
I. G. F. Goovaerts ◽  
V. H. Barnabe ◽  
P. E. J. Bols
Keyword(s):  
Lipid Peroxidation ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Membrane Lipid ◽  
Wilcoxon Test ◽  
Effect Of Temperature ◽  
Embryo Production ◽  
Sperm Membrane ◽  
In Vitro Embryo Production

Sperm recovery from the cauda epididymis can be a usefull tool in case of unexpected death of genetic high-value animals or endangered species or when the collection of sperm by other means becomes impossible. Studies indicate that the lower the temperature of epididymis storage, the better the sperm quality after collection (Kaabi et al. 2003 Theriogeneology 60, 1249-1259). One of the main factors that can negatively affect sperm viability during storage is lipid peroxidation, where sperm membrane resistance against reactive oxygen species (ROS) attacks is an important factor. The objective of this experiment was to study whether the temperature of epididymis storage following slaughter would have an influence on the membrane's resistance against lipid peroxidation and on the sperm cell's fertilizing capacity. Sixteen epididymides (from eight bulls) were collected after slaughter and divided into two groups, one stored at 4�C and the other at 37�C for 2 h, after which semen was collected from the caudae epididymides. Sperm concentration was measured and an aliquot containing 108 sperm cells was submitted to induced lipid peroxidation with ferrous sulfate and ascorbate (37�C; 2 h). Subsequently, thiobarbituric acid reactive substances (TBARS), as an index of lipid peroxidation, were measured according to a method previously described (Beorlegui et al. 1997 Andrologia 29, 37-42). A second aliquot of the sample was used for fertilization in a routine IVF-IVC set up in duplicate (24-h maturation, SOF culture medium in 5% CO2, 5% O2, and 90% N2). In vitro embryo production and level of TBARS were statistically analyzed using SAS (SAS Institute, Inc., Cary, NC, USA). TBARS levels were transformed to logarithm form in order to obey the residue normality being analyzed using PROC GLM. The percentage of blastocysts was analyzed using the Wilcoxon test. When compared to the samples stored at 4�C, semen of caudae epididymides stored at 37�C showed higher levels of TBARS and lower mean blastocyst rates (324.7 � 59.6 and 36.6 � 1.6 vs. 466.9 � 67.9 ng of TBARS/108 spermatozoa and 28.8 � 2.9%, respectively; P < 0.05). A negative correlation was found between TBARS and blastocyst rates (R = -0.43). The lower quality of sperm collected from epididymides maintained at higher temperatures may be related to a decrease in sperm resistance against lipid peroxidation which would further impair sperm fertilizing capacity. However, further studies are necessary in order to study the effect of temperature on the sperm membrane lipid profile, because the content of polyunsaturated fatty acids may be affected by temperature; this is an important factor relative to sperm membrane lipid peroxidation susceptibility (Ollero et al. 2000 Mol. Reprod. Dev. 55, 326-334). Another important factor is the epididymal environment because interactions between the sperm membrane and its surroundings can play an important role on the membrane's antioxidant protection.

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