scholarly journals Early Feeding Regime of Waste Milk, Milk, and Milk Replacer for Calves Has Different Effects on Rumen Fermentation and the Bacterial Community

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 443 ◽  
Author(s):  
Rong Zhang ◽  
Wei-bing Zhang ◽  
Yan-liang Bi ◽  
Yan Tu ◽  
Yves Beckers ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Rumen Fermentation ◽  
The Body ◽  
Milk Replacer ◽  
Early Feeding ◽  
Blood Indices ◽  
Molar Proportion ◽  
Waste Milk

We investigated the effects of different types of early feeding on rumen fermentation parameters and the bacterial community in calves. Fifty-four Holstein calves were assigned to three treatments and fed whole milk (M), pasteurized waste milk (WM), or milk replacer (MR). Male calves were slaughtered at the age of two months to measure the stomach masses. The female calves were followed for six months to determine the body weight, blood indices, rumen fermentation, and ruminal bacterial community. At the age of two months, the average daily gain was lower, but the concentration of total volatile fatty acids was greater in the MR group. Starter intake and stomach mass were lower, but the isovalerate molar proportion was greater in the WM group. The blood indices and ruminal bacterial community of the WM group differed from those of the other groups. At the age of six months, the ruminal propionate molar proportion was lower, but the ruminal pH and acetate/propionate ratio were greater in the MR group. In conclusion, calves fed WM had different rumen fermentation and bacterial community during the weaning period, whereas feeding MR produced a long-lasting effect on the rumen environment.

scholarly journals The effect of different physical forms of starter feed on rumen fermentation indicators and weight gain in calves after weaning

2017 ◽  
Vol 86 (3) ◽  
pp. 285-291 ◽  
Author(s):  
Leoš Pavlata ◽  
Ondřej Šťastník ◽  
Štěpánka Křivová ◽  
Hana Dočkalová ◽  
Lenka Sedláková ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Weight Gain ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Average Daily Gain ◽  
Rumen Fermentation ◽  
Total Volatile ◽  
Ruminal Acidosis ◽  
Molar Proportion ◽  
Rumen Ph

The aim of the study was to determine the effect of different physical forms of starter feed on rumen fermentation indicators of calves after weaning and their weight gain. The experiment was performed with Czech Fleckvieh calves after weaning. The calves were fed ad libitum completely pelleted starter feed or texturized starter feed with chopped straw. The rumen fluid samples were collected after a month of feeding the starter feeds. The calves were weighed monthly. The pH, total acidity, total volatile fatty acids, acetate, propionate, butyrate, lactic acid, ammonia and the number of rumen ciliate protozoa were determined in the rumen fluid samples. The calves receiving the starter feed with straw showed significantly higher rumen pH (6.24 ± 0.51 vs. 5.58 ± 0.30), total volatile fatty acids (98.02 ± 20.46 vs. 61.40 ± 26.51 mmol/l), molar proportion of acetate (61.20 ± 4.87 vs. 50.53 ± 4.66%), and the acetate:propionate ratio (2.38 ± 0.53 vs. 1.34 ± 0.18) and lower molar proportion of propionate (26.55 ± 4.48 vs. 37.92 ± 3.58%) compared with the calves receiving pelleted starter feed. Average daily gain of the calves did not differ significantly. The feeding of starter feed with chopped straw compared with the pelleted starter feed led to better development of the rumen fermentation evaluated by rumen pH, by total volatile fatty acids production, and by the proportion and ratio of acetic and propionic acids. The feeding of starter feed with chopped straw reduced the occurrence of subacute ruminal acidosis in the weaned calves.

scholarly journals Effects of Dietary Energy on Growth Performance, Rumen Fermentation and Bacterial Community, and Meat Quality of Holstein-Friesians Bulls Slaughtered at Different Ages

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1123 ◽  
Author(s):  
Haibo Wang ◽  
Hang Li ◽  
Fei Wu ◽  
Xinjun Qiu ◽  
Zhantao Yu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Growth Performance ◽  
Meat Quality ◽  
Energy Levels ◽  
Average Daily Gain ◽  
Rumen Fermentation ◽  
Dietary Energy ◽  
Different Ages ◽  
Slaughter Age

The objective of this study was to evaluate the effects of dietary energy levels on growth performance, rumen fermentation and bacterial community, and meat quality of Holstein-Friesians bulls slaughtered at different ages. Thirty-six Holstein-Friesians bulls (17 months of age) were divided into a 3 × 3 factorial experiment with three energy levels (LE, ME and HE; metabolizable energy is 10.12, 10.90 and 11.68 MJ/kg, respectively) of diets, and three slaughter ages (20, 23 and 26 months). Results indicated that bulls fed with ME and HE diets had higher dry matter intake, average daily gain, and dressing percentage at 23 or 26 months of age. The ME and HE diets also reduced bacterial diversity, altered relative abundances of bacteria and produced lower concentrations of acetate, but higher butyrate and valerate concentrations in rumen fluid. Increasing in dietary energy and slaughter age increased the intramuscular fat (IMF) and water holding capacity. In summary, Holstein-Friesians bulls fed with ME and HE diets, slaughtered at 23 and 26 months of age could be a good choice to produce beef with high IMF. Slaughter age may have less influence than dietary energy in altering fermentation by increasing amylolytic bacteria and decreasing cellulolytic bacteria, and thus, further affecting meat quality.

The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

2014 ◽  
Vol 54 (10) ◽  
pp. 1871 ◽  
Author(s):  
Arturo Samuel Gomez Insuasti ◽  
Yury Tatiana Granja Salcedo ◽  
Pablo de Souza Castagnino ◽  
Bruno Ramalho Vieira ◽  
Euclides Braga Malheiros ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Ether Extract ◽  
Microbial Protein ◽  
Molar Proportion ◽  
Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

Evaluation of ketogenic vs. glucogenic substrates as energy sources in starter diets for Holstein dairy calves

2014 ◽  
Vol 94 (4) ◽  
pp. 717-723 ◽  
Author(s):  
H. Beiranvand ◽  
M. Khorvash ◽  
G. R. Ghorbani ◽  
A. Homayouni ◽  
L. Bachmann ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Experimental Period ◽  
Dairy Calves ◽  
Energy Sources ◽  
Sodium Propionate ◽  
Waste Milk ◽  
Weaning Age ◽  
Calf Performance ◽  
Holstein Calves

Beiranvand, H., Khorvash, M., Ghorbani, G. R., Homayouni, A., Bachmann, L. and Kargar, S. 2014. Evaluation of ketogenic vs. glucogenic substrates as energy sources in starter diets for Holstein dairy calves. Can. J. Anim. Sci. 94: 717–723. The objective of this study was to investigate the influence of the inclusion of granular sugar and sodium propionate as ketogenic and glucogenic substrates, respectively, in calf starter diets on the performance, weaning age, rumen fermentation characteristics and plasma β-hydroxybutyrate (βHBA). Twenty-one newborn male Holstein calves were housed in individual pens until 10 wk of age, receiving starter ad libitum, fresh water free choice, and fed four liters of pasteurized waste milk daily until weaning. Calves were randomly assigned to one of the following treatments: (1) no supplemental additive (Control); (2) granular sugar (5% of dietary DM; Sugar); and (3) sodium propionate (5% of dietary DM; Propionate). Overall, no differences were observed among treatments for starter intake, average daily gain, feed efficiency, day of weaning, body weight at weaning and at the end of the study. Body measurements including body length, hip height, hip width, and heart girth were remained unchanged among treatments. Ruminal fluid pH (5.36 to 5.58) and concentrations of total volatile fatty acids (93.1 to 101.6 mM), acetate (43.6 to 53.1 mM), propionate (30.5 to 35.1 mM), and ratio of acetate to propionate (1.42 to 1.89) were not affected by treatments over the experimental period. However, the concentration of butyrate was greater at 35 and 70 d of age in calves receiving Sugar compared with calves receiving Propionate, but not compared with the Control. Greater blood βHBA at 45, 60 and 70 d of age was noticed in calves fed Sugar compared with those fed Control and Propionate. In conclusion, according to the concentrations of supplemental ketogenic and glucogenic substrates used in the current experiment, sugar and sodium propionate in starter diets did not improve calf performance.

scholarly journals Age-Related Changes in the Ruminal Microbiota and Their Relationship With Rumen Fermentation in Lambs

2021 ◽  
Vol 12 ◽  
Author(s):  
Xuejiao Yin ◽  
Shoukun Ji ◽  
Chunhui Duan ◽  
Peizhi Tian ◽  
Sisi Ju ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Bacterial Community ◽  
Growth Performance ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Rumen Bacteria ◽  
Ruminal Fermentation ◽  
Rumen Microbiota ◽  
Age Related ◽  
Age Related Changes

The rumen microbiota is vital for the health and growth performance of the host animal, mainly due to its role in the fermentation of ingested feed within the rumen. Attaining a better understanding of the development of the bacterial community and fermentation in the rumen can provide the theoretical basis for regulating feed utilization. This study analyzed the development of rumen bacteria in lambs from birth to 4 months of age using 16S-rRNA amplicon sequencing data and studied its relationship with ruminal fermentation. Serum levels of metabolites were monitored at 30, 60, 90, and 120 days of age, and the RandomForest approach was used to determine age-related changes in rumen bacteria. Levels of blood metabolites, ruminal fermentation, the rumen bacterial community and its functions were all affected by the age of the lambs (P < 0.05). Based on the Bray-Curtis distance within the age groups of the rumen microbiota, the similarity increased sharply after the lambs were weaned at 60 days of age (P < 0.05). The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age, increased after 20 days of age, reaching a maximum at 90 days vs. 120 days (P < 0.05). Some age-associated changes in the microbial genera were correlated with changes in the concentrations of volatile fatty acids and the levels of microbial crude protein in the rumen, including positive correlations between main volatile fatty acids and the genera of Prevotella 1, Lachnospiraceae NK3A20 group, Ruminococcus gauvreauii group, Ruminococcaceae UCG-014, and Ruminococcus 2 (P < 0.05). These results indicated that the microbial community and the function of rumen was not well-established before 20 days of age, so there is a degree of plasticity in the rumen bacterial community during the first 20 days of post-natal development in lambs, and this might provide an opportunity for interventions to improve rumen fermentation and, thus, increase their growth performance.

scholarly journals Increasing Buffering Capacity Alters Rumen Microbiota Composition and Enhances Rumen Fermentation Characteristics of High-Concentrate Fed Hanwoo Steers

2021 ◽  
Author(s):  
Sonny Ramos ◽  
Seon Ho Kim ◽  
Chang Dae Jeong ◽  
Lovelia L. Mamuad ◽  
A-rang Son ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Buffering Capacity ◽  
Ruminal Fermentation ◽  
Drastic Decrease ◽  
Degrading Bacteria

Abstract Background: Rumen bacterial community is mainly affected by the type of diet consumed by the host animals. High concentrate diet increases the abundance of lactic acid producers and utilizers due to high level of non-structural carbohydrates thus reducing the number of fiber-degrading bacteria because of drastic decrease in pH. Dietary buffers are essential in regulating rumen pH through the compounds responsible in resisting drastic decrease in pH once cattle were fed with high-concentrate diet. However, no study has evaluated the effects of buffering capacity and efficiency in alleviating chronic acidosis in rumen. Ruminal metataxonomic and fermentation characteristics analyses were conducted to evaluate the effect of different buffering capacities on in vitro and in vivo experiments in high-concentrate fed Hanwoo steers. Results: Results revealed that BC0.9% and BC0.5% had similar and significant effect (P < 0.05) on in vitro ruminal fermentation at 3 to 24 h incubation. Both BC0.9% and BC0.5% had significantly highest (P < 0.05) buffering capacity, pH, and ammonia-nitrogen (NH3-N) than BC0.3% and CON at 24 h of incubation. Individual and total volatile fatty acids (VFA) were significantly lowest in CON. Increasing buffering capacity concentration showed linear effect on pH at 6 to 24 h while total gas and NH3-N at 3 and 12 h. Phylum Bacteroidetes dominated all treatments but a higher abundance of Firmicutes in BC0.5% than others. Ruminoccocus bromii and Succiniclasticum ruminis were dominant in BC0.5% and Bacteroides massiliensis in BC0.3%. The normalized data of relative abundance of observed OTUs’ representative families have grouped the CON with BC0.3% in the same cluster, whereas BC0.5% and BC0.9% were clustered separately which indicates the effect of varying buffering capacity of buffer agents. Principal coordinate analysis (PCoA) on unweighted UniFrac distances revealed close similarity of bacterial community structures within and between treatments and control, in which BC0.9% and BC0.3% groups showed dispersed community distribution. Conclusion: Our findings showed that increasing buffering capacity enhances rumen fermentation parameters and affects rumen microbiome by altering bacterial community through distinct structure between high and low buffering capacity, thus an important factor contributed to the prevention of ruminal acidosis during a high-concentrate diet.

scholarly journals Different Methods of Eubiotic Feed Additive Provision Affects Health, Performance, Fermentation, and Metabolic Status of Dairy Calves During The Preweaning Period

2021 ◽  
Author(s):  
Barbara Stefańska ◽  
Frank Katzer ◽  
Barbara Golińska ◽  
Sebastian Smulski ◽  
Patrycja Sobolewska ◽  
...  
Keyword(s):  
Experimental Period ◽  
Rumen Fermentation ◽  
Feed Additives ◽  
The Body ◽  
Dairy Calves ◽  
Metabolic Status ◽  
Feed Additive ◽  
Milk Replacer ◽  
Health Performance ◽  
Preweaning Period

Abstract Background The aim of this study was to evaluate whether different methods of providing eubiotic feed additives, to neonatal calves during the preweaning period, can improve the health, performance, rumen fermentation, and metabolic status of the calves. Forty-four Holstein-Friesian dairy calves, were divided into one of four treatment groups for the 8 week trial. The eubiotic feed additives consisted of a combination of probiotic Lactobacillus spp. (multiple-strains) at a dose of 250 mg/calf/d and phytobiotics containing rosmarinic acid, as the main bioactive compound, at a dose of 50 mg/calf/d. Treatment differed by the methods that the eubiotic feed additives were provided, and the groups were as follows: CON (control, without eubiotic feed additive in their milk replacer or their starter feed), MR (eubiotic feed additives added to the milk replacer), SF (eubiotic feed additives added to the starter feed), MRS (eubiotic feed additives added to the milk replacer and the starter feed). Individual intake of starter feed was measured daily and body weights weekly until 56 d of age. Results The body weight of the MR treatment group calves were higher on days 28 and 56. Including the eubiotic feed additive in the milk replacer affected increasing average daily gain, starter intake and total dry matter intake from d 29 to d 56 and the overall experimental period in comparison to the CON group. The calves of the MR treatment had lower fecal scores from d 3 to d 28, number of parasite oocysts/cysts per gram of feces on d 28, and the occurrences of faecal consistency scores of 3 (mild diarrhea) and 4 (confirmation diarrhea) were 3.2 and 3.0 times lower, respectively, compared with animals of the CON group. Also, in this group higher ruminal concentrations of total volatile fatty acids, propionate, and butyrate were noted on d 56 compared to CON group. Adding eubiotics into milk replacer resulted the highest concentrations of blood insulin-like growth factor-I and β-hydroxybutyrate from d 29 to 56 and through the entire experimental period. Conclusion The addition of eubiotic feed additives into the milk replacer can improve health, performance, rumen fermentation, and biochemical blood indices in dairy calves during the preweaning period.

scholarly journals Addition of calcium propionate to finishing lamb diets

2019 ◽  
Vol 5 (4) ◽  
Author(s):  
José A. Martínez-Aispuro ◽  
María Teresa Sánchez-Torres ◽  
German D. Mendoza-Martínez ◽  
José L. Cordero Mora ◽  
José L. Figueroa-Velasco ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Optimal Dose ◽  
Rumen Fermentation ◽  
Finishing Diets ◽  
Randomized Design ◽  
Ruminant Diets ◽  
Quadratic Response ◽  
Calcium Propionate ◽  
Daily Gain

Calcium (Ca) propionate can be added to ruminant diets as a glucogenic substrate. However, due to its hypophagic effect, it is necessary to establish the optimal dose that can be used to replace grains in finishing diets for lambs. Therefore, the objective of this study was to assess the effect of four concentrations of Ca propionate in lamb diets on productive performance and rumen fermentation. Thirty two Hampshire x Suffolk lambs (23.82 ± 0.40 kg initial body weight), distributed in a completely randomized design, were given a diet with four concentrations of Ca propionate (g kg−1): 0, 10, 20 or 30 g, for 42 days. The results were tested for linear or quadratic responses. The final weight, average daily gain and feed: gain ratio showed quadratic responses (P ≤ 0.01). The optimal dose was established at 13.77 g kg−1 DM. Addition of Ca propionate did not affect variables related to rumen fermentation (pH, total volatile fatty acids, acetate, butyrate or rumen ammonia-N; P ≥ 0.05) . Nonetheless, glucose and propionate concentrations showed a quadratic response (P ≤ 0.05). The highest concentrations of propionate in rumen were observed with 15.14 g kg−1 DM. Results indicate that Ca propionate can be included in a dose of up to 13.77 g kg−1 DM in feedlot rations, to attain best lamb performance.

scholarly journals Effect of level of monensin on rumen fermentation characteristics in Awassi lambs

2020 ◽  
Vol 11 (2) ◽  
pp. 1-11
Author(s):  
Razzaq Baiee ◽  
◽  
Ali Saeed ◽  
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
The Body ◽  
Control Treatment ◽  
Average Weight ◽  
College Of Agriculture ◽  
Experimental Treatments ◽  
The Individual ◽  
Ground Wheat ◽  
Awassi Lambs

The current study was conducted in the Animal field of the Department of Animal Production – College of Agriculture - Al-Qasim Green University for the period from 10/10/2019 to 19/1/2020 to investigate the effect of addition of monensin at levels of 15, 30 and 45 mg/kg concentrate on growth performance of Awassi lambs. First treatment in which lambs were fed concentrate diet without addition of monensin was considered the control treatment. Sixteen male Awassi lambs were used with an average weight of 24.85 and 4-6 months of age. The lambs were randomly distributed to the experimental treatments, four lambs per each using the individual feeding in the cages. The concentrate diet was offered at a level of 2.75% of the body weight at 2 meals, morning and evening meals, while the ground wheat straw was offered at free choice. Results revealed no significant effect of level of monensin on pH values, whereas ruminal ammonia concentrations were significantly decreased (P˂0.05) from 7.54 in the control treatment to 6.08 and 6.45 mg/100 ml due to addition of monensin at levels of 30 and 45 mg/kg. Moreover, addition of monensin at a level of 30 mg/kg concentrate diet significantly increased (P˂0.05) ruminal concentration of total volatile fatty acids to 13.76 as compared with 10.99 mM/100 ml for control treatment. Concentrations of all rumen fermentation characteristics were significantly affected (P˂0.01) by time of ensiling.

scholarly journals Comparative study of growth performance, nutrient digestibility, and ruminal and fecal bacterial community between yaks and cattle-yaks raised by stall-feeding

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qindan Dai ◽  
Jian Ma ◽  
Guang Cao ◽  
Rui Hu ◽  
Yixiao Zhu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Growth Performance ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Energy Utilization ◽  
Acid Detergent Fiber ◽  
Fecal Bacterial Community ◽  
Daily Gain

AbstractThe experiment was conducted to compare the growth performance, rumen fermentation, nutrient digestibility, and ruminal and fecal bacterial community between yaks and cattle-yaks. Ten male yaks (36-month-old) were used as the yak (YAK) group and 10 male cattle-yaks with similar age were selected as the cattle-yak (CAY) group. All the animals were fed same ration and the experiment lasted for 60 days. The results showed that the average daily gain and dry matter intake of CAY group were higher (P < 0.05) than those of YAK group. The ruminal concentrations of total volatile fatty acids, acetate, and butyrate were higher (P < 0.05) in CAY group than those in YAK group. However, the neutral detergent fiber and acid detergent fiber digestibility exhibited an opposite between two groups. In the rumen, the relative abundances of Prevotella 1 and Prevotellaceae UCG-001 were higher (P < 0.05) and Succiniclasticum and Butyrivibrio 2 were lower (P < 0.05) in YAK group compared to CAY group. In the feces, the unclassified Lachnospiraceae, Lachnospiraceae NK4A136 group, and Lachnospiraceae AC2044 group were significantly enriched (P < 0.05) in YAK group, whereas the Ruminococcaceae UCG-010, Ruminococcaceae UCG-013, and Succiniclasticum were significantly enriched (P < 0.05) in CAY group. Overall, under the same diet, the yaks have higher fiber utilization and cattle-yaks have higher energy utilization.

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