scholarly journals Cecal Infusion of Sodium Propionate Promotes Intestinal Development and Jejunal Barrier Function in Growing Pigs

Animals ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 284 ◽  
Author(s):  
Yanan Zhang ◽  
Huizi Chen ◽  
Weiyun Zhu ◽  
Kaifan Yu
Keyword(s):  
Tight Junction ◽  
Saline Group ◽  
Growing Pigs ◽  
Intestinal Morphology ◽  
Tight Junction Protein ◽  
Short Chain ◽  
Intestinal Development ◽  
Sodium Propionate ◽  
Propionate Group ◽  
Junction Protein

Short-chain fatty acids (SCFAs) produced by microbial fermentation facilitate the differentiation and proliferation of intestinal epithelium. However, the role of individual SCFAs, such as propionate, on intestinal development is still unclear. In the present study, sixteen barrows fitted with a cecal fistula were randomly divided into two groups for cecal infusion of either saline (control group) or sodium propionate (propionate group). After 28 days, the length and the relative weight of intestinal segments were calculated, the intestinal morphology was assessed, and the expression of tight junction protein was measured using qPCR and Western blotting. Compared to the saline group, the length of the colon was significantly increased in the propionate group (p < 0.05). The jejunal villi length and villi/crypt ratio in the propionate group were significantly higher than in the saline group (p < 0.05). Furthermore, propionate infusion significantly upregulated the mRNA levels of Claudin-4 and the expression of Claudin-1, Claudin-4, and Occludin protein in the jejunal mucosa (p < 0.05). Collectively, these findings revealed that the short-chain fatty acid propionate in the hindgut contributed to intestinal development, and selectively enhanced jejunal tight junction protein expression.

scholarly journals Erratum to “Effect of High Dietary Tryptophan on Intestinal Morphology and Tight Junction Protein of Weaned Pig”

2017 ◽  
Vol 2017 ◽  
pp. 1-1
Author(s):  
Myrlene Carine B. Tossou ◽  
Hongnan Liu ◽  
Miaomiao Bai ◽  
Shuai Chen ◽  
Yinghua Cai ◽  
...  
Keyword(s):  
Tight Junction ◽  
Intestinal Morphology ◽  
Tight Junction Protein ◽  
Junction Protein ◽  
Weaned Pig

scholarly journals Polycystin-2 Activity Is Controlled by Transcriptional Coactivator with PDZ Binding Motif and PALS1-associated Tight Junction Protein

2010 ◽  
Vol 285 (44) ◽  
pp. 33584-33588 ◽  
Author(s):  
Kerstin Duning ◽  
Deike Rosenbusch ◽  
Marc A. Schlüter ◽  
Yuemin Tian ◽  
Karl Kunzelmann ◽  
...  
Keyword(s):  
Tight Junction ◽  
Tight Junction Protein ◽  
Binding Motif ◽  
Transcriptional Coactivator ◽  
Junction Protein

scholarly journals Endothelial-specific insulin receptor substrate-1 overexpression worsens neonatal hypoxic-ischemic brain injury via mTOR-mediated tight junction disassembly

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yi-Fang Tu ◽  
Si-Tse Jiang ◽  
Chi-Wu Chiang ◽  
Li-Ching Chen ◽  
Chao-Ching Huang
Keyword(s):  
Endothelial Cells ◽  
Brain Injury ◽  
Tight Junction ◽  
Insulin Receptor ◽  
Vascular Endothelial Cells ◽  
Tight Junction Protein ◽  
Insulin Receptor Substrate ◽  
Vascular Endothelial ◽  
Transgenic Rats ◽  
Junction Protein

AbstractHypoxic-ischemic (HI) encephalopathy is the major cause of mortality and disability in newborns. The neurovascular unit is a major target of acute and chronic brain injury, and therapies that protect simultaneously both neurons and vascular endothelial cells from neonatal HI injury are in demand. Insulin receptors and its key downstream molecule-insulin receptor substrate −1 (IRS-1) are potential neuroprotective targets and expressed both in neuron and endothelial cells. To investigate whether IRS-1 can act similarly in neurons and vascular endothelial cells in protecting neurovascular units and brain form HI injury, we found that neuron-specific IRS-1 transgenic rats showed reduced neurovascular injury and infarct volumes, whereas endothelial-specific IRS-1 transgenic rats showed increased blood-brain barrier (BBB) disruption and exaggerated neurovascular injury after neonatal HI brain injury. Endothelial-specific IRS-1 overexpression increased vascular permeability and disassembled the tight junction protein (zonula occludens-1) complex. Inhibition of mammalian target of rapamycin (mTOR) by rapamycin preserved tight junction proteins and attenuated BBB leakage and neuronal apoptosis after HI in the endothelial-specific IRS-1 transgenic pups. Together, our findings suggested that neuronal and endothelial IRS-1 had opposite effects on the neurovascular integrity and damage after neonatal HI brain injury and that endothelial IRS-1 worsens neurovascular integrity after HI via mTOR-mediated tight junction protein disassembly.

The Tight Junction Protein Cingulin Regulates Gene Expression and RhoA Signaling

2009 ◽  
Vol 1165 (1) ◽  
pp. 88-98 ◽  
Author(s):  
Sandra Citi ◽  
Serge Paschoud ◽  
Pamela Pulimeno ◽  
Francesco Timolati ◽  
Fabrizio De Robertis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tight Junction ◽  
Tight Junction Protein ◽  
Junction Protein ◽  
Rhoa Signaling

Changes in tight junction protein expression in intrinsic aging and photoaging in human skin in vivo

2016 ◽  
Vol 84 (1) ◽  
pp. 99-101 ◽  
Author(s):  
Seon-Pil Jin ◽  
Sang Bum Han ◽  
Yeon Kyung Kim ◽  
Elizabeth Eunkyung Park ◽  
Eun Jin Doh ◽  
...  
Keyword(s):  
Protein Expression ◽  
Tight Junction ◽  
Human Skin ◽  
Tight Junction Protein ◽  
Tight Junction Protein Expression ◽  
Junction Protein

scholarly journals Hyaluronan 35 kDa treatment protects mice from Citrobacter rodentium infection and induces epithelial tight junction protein ZO-1 in vivo

2017 ◽  
Vol 62 ◽  
pp. 28-39 ◽  
Author(s):  
Yeojung Kim ◽  
Sean P. Kessler ◽  
Dana R. Obery ◽  
Craig R. Homer ◽  
Christine McDonald ◽  
...  
Keyword(s):  
Tight Junction ◽  
Tight Junction Protein ◽  
Citrobacter Rodentium ◽  
Junction Protein ◽  
Epithelial Tight Junction
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