scholarly journals Effects of Sulfur Levels in Fermented Total Mixed Ration Containing Fresh Cassava Root on Feed Utilization, Rumen Characteristics, Microbial Protein Synthesis, and Blood Metabolites in Thai Native Beef Cattle

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 261 ◽  
Author(s):  
Chanadol Supapong ◽  
Anusorn Cherdthong ◽  
Metha Wanapat ◽  
Pin Chanjula ◽  
Sutipong Uriyapongson
Keyword(s):  
Protein Synthesis ◽  
Beef Cattle ◽  
Latin Square ◽  
Hydrocyanic Acid ◽  
Blood Metabolites ◽  
Microbial Protein ◽  
Bacterial Populations ◽  
Total Mixed Ration ◽  
Microbial Protein Synthesis ◽  
Cassava Root

The influence of sulfur included in fermented total mixed ration (FTMR) containing fresh cassava root on rumen characteristics, microbial protein synthesis, and blood metabolites in cattle was evaluated. Four Thai native beef cattle were randomly assigned according to a 2 × 2 factorial in a 4 × 4 Latin square design, and dietary treatments were as follows: factor A included a level of sulfur at 1% and 2% in total mixed ration, and factor B featured ensiling times at zero and 7 days. Digestibility of dry matter was increased when FTMR was supplemented with 2% sulfur. Blood thiocyanate increased by 69.5% when ensiling time was 7 days compared to no ensiling (p < 0.01). Bacterial populations were significantly different in the FTMR containing sulfur at 2% and 7 days of ensiling. Furthermore, microbial crude protein and efficiency of microbial protein synthesis were higher in the FTMR containing 2% sulfur and 7 days of ensiling (p < 0.01). Thus, high levels of hydrocyanic acid from fresh cassava root could be detoxified by a sulfur addition with an ensiling process to become nontoxic to cattle.

scholarly journals Effect of soybean meal replacement by slow-release urea on ruminal parameter, blood metabolites, and microbial protein synthesis in Zel ram

2020 ◽  
Vol 43 ◽  
pp. e48684
Author(s):  
Zahra Alizadeh ◽  
Asadollah Teymouri Yansari ◽  
Yadollah Chashnidel ◽  
Mohammad Kazemifard ◽  
Samaneh Azarpajouh
Keyword(s):  
Protein Synthesis ◽  
Soybean Meal ◽  
Slow Release ◽  
Latin Square ◽  
Blood Metabolites ◽  
Microbial Protein ◽  
Meal Replacement ◽  
Microbial Protein Synthesis ◽  
Basic Diet ◽  
Slow Release Urea

The objective of this study was to determine the effect of soybean meal replacement by slow-release urea on feed intake, ruminal parameters, blood metabolites, dry matter, nutrients digestion, and microbial protein synthesis (MPS) in ram. Rams were used for four 21-day periods. The experimental design was a 4×4 Latin square design. The experiment has consisted of the following four rations: 1) basic diet with soybean meal as a protein source, 2) basic diet with soybean meal plus 0.5 % of DM slow-release urea (SRU), 3) basic diet with soybean meal plus 1 % of DM SRU, and 4) basic diet with soybean meal plus 1.5 % of SRU, respectively. Soybean meal replacement by SRU decreased ruminal ammonia and blood urea. SRU increase in diets significantly increased acetic acid 3 hours after morning feeding, blood glucose, total purine, uric acid excretion, microbial nitrogen, and microbial protein. The results showed that soybean meal replacement by SRU sources had no negative effect on rams performance.

scholarly journals Strategic Supplementation of Flemingia Silage to Enhance Rumen Fermentation Efficiency, Microbial Protein Synthesis and Methane Mitigation in Beef Cattle

2020 ◽  
Author(s):  
Bounnaxay Viennasay ◽  
Metha Wanapat
Keyword(s):  
Protein Synthesis ◽  
Beef Cattle ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Microbial Protein ◽  
Fatty Acid Production ◽  
Microbial Protein Synthesis ◽  
Dietary Treatments ◽  
Nutrients Digestibility

Abstract Background: Good quality protein as an on-farm feed resources has been in great demand to support the productivity of ruminants. A digestion trial using beef cattle crossbreds was conducted to assess the four dietary treatments of Flemingia macrophylla silage (FMS) supplementation at 0, 0.2, 0.4 and 0.6 kg dry matter (DM)/day in a 4 × 4 Latin square design. Feed DM intakes were measured during the 14 days and sample of feeds, feces, urine, as well as rumen fluid, blood were collected during the 7 days while the animals were on metabolism crates.Results: Based on this experiment revealed that strategic supplementation of FMS increased (P < 0.05) nutrients digestibility (organic matter, crude protein, and acid detergent fiber) enhanced rumen total volatile fatty acid production especially propionic acid (C3), C2:C3 ratio while, remarkably promoted the microbial protein synthesis (MPS) by increasing N-balance and retention of purine derivatives.Conclusion: Under this experiment, the results revealed the potential use of FMS as a good-quality feed to improve nutrients digestibility, rumen fermentation, microbial protein synthesis, and to mitigate methane production. FMS supplementation at 0.6 kg DM/day exhibited the best result.

scholarly journals Strategic supplementation of Flemingia silage to enhance rumen fermentation efficiency, microbial protein synthesis and methane mitigation in beef cattle

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Bounnaxay Viennasay ◽  
Metha Wanapat
Keyword(s):  
Protein Synthesis ◽  
Beef Cattle ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Microbial Protein ◽  
Fatty Acid Production ◽  
Microbial Protein Synthesis ◽  
Dietary Treatments ◽  
Nutrients Digestibility

Abstract Background Good quality protein as an on-farm feed resource has been in great demand to support the productivity of ruminants. A digestion trial using beef cattle crossbreds was conducted to assess the four dietary treatments of Flemingia macrophylla silage (FMS) supplementation at 0, 0.2, 0.4 and 0.6 kg dry matter (DM)/day in a 4 × 4 Latin square design. Feed DM intakes were measured during the 14 days and sample of feeds, feces, urine, as well as rumen fluid and blood were collected during the 7 days while the animals were on metabolism crates. Results Based on this experiment strategic supplementation of FMS increased (P < 0.05) nutrients digestibility (organic matter, crude protein, and acid detergent fiber) enhanced rumen total volatile fatty acid production especially propionic acid (C3), C2:C3 ratio while, remarkably promoted the microbial protein synthesis (MPS) by increasing N-balance and retention of purine derivatives. Conclusions Under this experiment, the results revealed the potential use of FMS as a good-quality feed to improve nutrients digestibility, rumen fermentation, microbial protein synthesis, and to mitigate methane production. FMS supplementation at 0.6 kg DM/day exhibited the best result.

scholarly journals Microbial protein synthesis and nitrogen metabolism in cows bred on tropical pasture and fed on cassava root and corn

2014 ◽  
Vol 36 (2) ◽  
pp. 185 ◽  
Author(s):  
Ádler Carvalho da Silva ◽  
Mauro Pereira de Figueiredo ◽  
Paulo Bonomo ◽  
Mara Lúcia Albuquerque Pereira ◽  
Yann Dos Santos Luz ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Nitrogen Metabolism ◽  
Tropical Pasture ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Cassava Root

Effect of altering the non-structural: structural carbohydrate ratio in a pasture diet on milk production and ruminal metabolites in cows in early and late lactation

1997 ◽  
Vol 64 (3) ◽  
pp. 393-402 ◽  
Author(s):  
V. R. Carruthers ◽  
P. G. Neil ◽  
D. E. Dalley
Keyword(s):  
Protein Synthesis ◽  
Milk Production ◽  
Milk Yield ◽  
Latin Square ◽  
Carbohydrate Intake ◽  
Microbial Protein ◽  
P Efficiency ◽  
Total Carbohydrate ◽  
Microbial Protein Synthesis ◽  
Digestible Organic Matter

AbstractThe effect on digestibility, ruminal metabolites, microbial protein synthesis and milk production of manipulating the non-structural (NSC): structural (SO carbohydrate ratio in a predominantly pasture diet was investigated in cows in early (trial 1) and late (trial 2) lactation. Twenty-four cows in trial 1 and 15 cows in trial 2 were offered pasture only (P), 0·85 P plus 0·15 NSC/protein mixture (PR), and P plus an additional 0·1 (trial 1) or 0·15 (trial 2) NSC (PE) in a Latin-square arrangement. All diets were isonitrogenous and P and PR were isoenergetic. PE but not PR increased microbial protein synthesis and decreased ruminal ammonia and milk urea levels, compared with P. Efficiency of microbial synthesis (g N per kg digestible organic matter intake) was not altered by treatment. Treatments had minor effects on ruminal pH and no effect on volatile fatty acid concentrations. PE and PR did not affect milk yield or protein yield and decreased fat yield compared with P in trial 1. Milk yield was increased on PE and PR compared with P and was greater on PE than PR, in trial 2. Yields of fat and protein were higher on PE than on P and yield of protein was higher on PR than on P. The results suggest that increasing the ratio of NSC: protein by increasing total carbohydrate intake was more effective in improving nitrogen utilization in the rumen than was increasing the NSC: SC ratio without increasing carbohydrate intake.

scholarly journals Effects of Supplementation with Royal Poinciana Seed Meal (Delonix regia) on Ruminal Fermentation Pattern, Microbial Protein Synthesis, Blood Metabolites and Mitigation of Methane Emissions in Native Thai Beef Cattle

Animals ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 625 ◽  
Author(s):  
Anusorn Cherdthong ◽  
Rittikeard Prachumchai ◽  
Metha Wanapat ◽  
Suban Foiklang ◽  
Pin Chanjula
Keyword(s):  
Protein Synthesis ◽  
Acetic Acid ◽  
Beef Cattle ◽  
Propionic Acid ◽  
Rice Straw ◽  
Crude Protein ◽  
Blood Metabolites ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Fermentation Efficiency

The object of this present work was to determine the effects of supplementation with pellets containing royal poinciana seed meal (PEREM) on feed use, ruminal fermentation efficiency, microbial protein synthesis, blood metabolites and mitigation of methane (CH4) emissions in cattle. The animals used in this experiment were four male Thai native beef cattle (Bos indicus) with initial body weights (BWs) of 125 ± 5.0 kg. Each of the animals were randomly assigned to receive PEREM doses at 0, 50, 100 and 150 g/d, respectively, according to a 4 × 4 Latin square design. Concentrates were fed at 0.5% BW daily, and rice straw was fed ad libitum. There were no significant differences (p > 0.05) on intakes of rice straw, concentrate and total diet. The intake of nutrients did not change among the levels of PEREM supplementation (p > 0.05), except for an intake of crude protein, which was linearly enhanced when increasing the dose of PEREM (p < 0.05). The inclusion of different doses of PEREM did not adversely affect the digestibility of dry matter, organic matter, crude protein, neutral detergent fiber and acid detergent fiber (p > 0.05). Adding various doses of PEREM did not alter ruminal pH and ruminal temperature, while concentrations of ammonia-nitrogen were significantly increased with an increased dose of PEREM supplementation (p < 0.01). The increasing doses of PEREM linearly reduced protozoal numbers (p < 0.01), with the lowest concentration when PEREM was added at 150 g. PEREM supplementation did not change (p > 0.05) the concentration of acetic acid or butyric acid or the ratio of acetic acid to propionic acid. Nevertheless, the total volatile fatty acid and propionic acid content were changed among PEREM levels (p < 0.05), which were linearly increased with an increasing dose of PEREM. At 4 h post feeding, the CH4 concentrations in the rumen of the animal were linearly reduced when the dose of pellets was increased (p < 0.01). In addition, the inclusion of PEREM did not adversely affect other blood metabolites, namely total protein, creatinine and albumin (p > 0.05). Furthermore, microbial crude protein and efficiency of microbial N synthesis were linearly enhanced when increasing levels of PEREM were added. The feeding of PEREM at 150 g/d might be an alternative with the potential to improve rumen fermentation efficiency and reduce the environmental effects produced by ruminants.

scholarly journals Inclusion of copaiba oil (Copaifera sp.) as additive in supplements for cattle on pasture

2018 ◽  
Vol 19 (2) ◽  
pp. 178-192
Author(s):  
Fabiola Espindola Ortega de Lima ◽  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Jefferson Rodrigues Gandra ◽  
Diego dos Santos Penha ◽  
Raquel Tenório de Oliveira ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Crude Protein ◽  
Polynomial Regression ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Microbial Protein ◽  
Significance Level ◽  
Microbial Protein Synthesis ◽  
Ruminal Ph ◽  
Copaiba Oil

SUMMARY This study analyzed the effect of including copaiba oil as an additive for cattle supplemented on pasture, during the dry season. Four crossbred steers, castrated, with 245 ± 25 kg, aged about 18 months, fitted with permanent rumen cannula; were randomly assigned to a 4×4 Latin square. All animals were housed in individual paddocks (0.3 ha), uniformly covered with Brachiaria brizantha cv Marandu, with through and drinking fountain; and were given a concentrate at 500g/100 kg BW containing 380 g/kg crude protein (%DM). The copaiba oil was added to the supplement as a spray, in the proportions of 0, 0.5; 1.0 and 1.5 g/kg DM intake. Spraying supplementation was performed daily at the time of supply of the supplement. Data of intake and digestibility of nutrients, ruminal pH; ruminal ammonia nitrogen, and microbial protein synthesis were tested by analysis of variance and polynomial regression, adopting a significance level of 5%. The inclusion of copaiba oil quadratically affected total dry matter intake (P=0.030) and CP digestibility (P=0.043), without altering ruminal metabolism (P>0.05) and microbial protein synthesis (P>0.05) of the animals kept on pasture.

scholarly journals Nitrogen balance, microbial protein synthesis and blood metabolites in fattening of male Bali cattle fed ration with different protein levels in smallholder farms

2018 ◽  
Vol 43 (1) ◽  
pp. 43 ◽  
Author(s):  
P. K. Tahuk ◽  
S. P. S. Budhi ◽  
P. Panjono ◽  
E. Baliarti
Keyword(s):  
Protein Synthesis ◽  
Blood Glucose ◽  
Nitrogen Balance ◽  
Blood Metabolites ◽  
Microbial Protein ◽  
Smallholder Farms ◽  
Microbial Protein Synthesis ◽  
Protein Levels ◽  
Before And After ◽  
Bali Cattle

Research was aimed to determine nitrogen balance, microbial protein synthesis, and blood metabolites of male Bali cattle fattening fed ration with different protein level in smallholder farms North Central Timor, Province of East Timor Tenggara, Indonesia. The cattle used were 18 heads aged 2 to 2.5 years with initial body weight of 229.86±12.46 kg. The cattle were randomly divided into three treatment groups. The T0 group was given feed the same as traditional fattening cattle practices by farmers,T1 group fed ration containing 12% crude protein (CP) and 72% total digestible nutrients (TDN), andT2 group fedration containing 15% CP and 72%TDN. Cattle were fed individually for 90 days and drinkingwater ad libitum. The data were analyzedby analysis of variance.Results of research indicated the nitrogen balance, and blood urea nitrogen between T1 and T2 were relatively similar, but those were higher (P<0.05) than T0 . In contrast, microbial proteins synthesis, and blood glucose at 0, 4, and 6 hours before and after feeding were relatively similar between the groups. Blood glucose of T2 at 2 hours after intake were higher (P <0.05) than T0, but was not different with T1 . It can be concluded, that the fattening maleBali cattle fed ration containing 12% CP and 72% TDNimprovedthe nitrogen balance and blood metabolites, butit was no positive effect on the microbial proteins and N synthesis.

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