scholarly journals Variation of Detailed Protein Composition of Cow Milk Predicted from a Large Database of Mid-Infrared Spectra

Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 176 ◽  
Author(s):  
Marco Franzoi ◽  
Giovanni Niero ◽  
Giulio Visentin ◽  
Mauro Penasa ◽  
Martino Cassandro ◽  
...  
Keyword(s):  
Infrared Spectra ◽  
Protein Composition ◽  
Protein Fractions ◽  
Cow Milk ◽  
Fat Percentage ◽  
Dual Purpose ◽  
Factors Affecting ◽  
Dual Purpose Cattle ◽  
Milk Urea ◽  
Β Lactoglobulin

This study aimed to investigate factors affecting protein fractions, namely α-casein (α-CN), β-casein (β-CN), κ-casein (κ-CN), β-lactoglobulin (β-LG) and α-lactalbumin (α-LA) predicted from milk infrared spectra in milk of dairy and dual-purpose cattle breeds. The dataset comprised 735,328 observations from 49,049 cows in 1782 herds. Results highlighted significant differences of protein fractions in milk of the studied breeds. Significant variations of protein fractions were found also through parities and lactation, with the latter thoroughly influencing protein fractions percentage. Interesting correlations (r) were estimated between β-CN, κ-CN and β-LG, expressed as percentage of crude protein, and milk urea nitrogen (r = 0.31, −0.20 and −0.26, respectively) and between α-LA and fat percentage (r = 0.41). The present study paves the way for future studies on the associations between protein fractions and milk technological properties, and for the estimation of genetic parameters of predicted protein composition.

Genetic and non-genetic factors affecting productive and reproductive traits in Deoni cattle

2018 ◽  
Author(s):  
Sukanta Basak ◽  
D. N. Das ◽  
U. T. Mundhe
Keyword(s):  
Milk Yield ◽  
Genetic Factors ◽  
Cattle Breed ◽  
Reproductive Traits ◽  
Phenotypic Correlation ◽  
Dual Purpose ◽  
Factors Affecting ◽  
Lactation Length ◽  
Service Period ◽  
Dual Purpose Cattle

The present study was carried out in Deoni, the dual purpose cattle breed of Southern India, maintained at NDRI herd, SRS, Bengaluru. Mean phenotypic performances for the traits of age at first calving (AFC), first service period (FSP), first lactation milk yield (FLMY) and first lactation length (FLL) were estimated as 42.90 ± 0.49 months, 179.00 ± 6.3 days, 643.08 ± 25.16 kg and 178.24 ± 4.41 days respectively. Significant effect of parity was found on AFC and FSP. Effect of period of birth, period of calving and season of calving was found to be significant on FSP. Significant effect of period of birth, period of calving, classes of AFC and FLL were found on FLMY. The heritability of first lactation milk yield was estimated as 0.38 ± 0.29. Phenotypic correlation of FLMY with FLL (0.88 ± 0.02) was found to be highly significant (p Lass Than 0.01).

scholarly journals Apparent Nitrogen Recovery in Milk and Early Dry Season Nitrous Oxide Emission Factors for Urine Deposited by Dual-Purpose Cattle on Different Soil Types

2021 ◽  
Vol 4 ◽  
Author(s):  
Sandra Guisela Durango Morales ◽  
Rolando Barahona ◽  
Diana M. Bolívar ◽  
Jacobo Arango ◽  
Louis Verchot ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
N Recovery ◽  
Tier 2 ◽  
N Losses ◽  
Dual Purpose ◽  
Tropical Conditions ◽  
Tier 1 ◽  
Dual Purpose Cattle ◽  
Milk Urea ◽  
Apparent N Recovery

Pasture conditions influence the nutrients use efficiency and nitrogen (N) losses from deposited excreta. Part of the N is lost as nitrous oxide (N2O), a potent greenhouse gas. The objective of this study was to characterize apparent N recovery in milk of dual-purpose cattle and to quantify N2O emissions from the urine they deposit following grazing on Megathyrsus maximus cv. Mombasa. The N content in the grass and the milk produced by the cattle and the milk urea N (MUN) content were quantified in two contrasting regions of Colombia (Casanare and Atlántico). Dry matter intake (DMI) by the cattle was estimated using the Cornell Net Carbohydrate and Protein System. We used a closed static chamber technique to measure N2O emissions from soils in areas with and without urine patches (21 days in Atlántico and 35 Days in Casanare). Estimated DMI values were 11.5 and 11.6 kg DM day−1, milk production was 6.5 and 5.9 L day−1, apparent N recovery in milk was 24 and 23%, and the MUN content was 4.4 and 17.2 mg N dl−1 in Casanare and Atlántico, respectively. N applied to soil in the form of urine corresponded at rates of 20 and 64 g N m−2 and net cumulative N2O emissions were 350 and 20 mg N2O-N m−2 in Casanare and Atlántico, respectively. Despite low digestibility of offered diet, N recovery in milk was above the values reported at dairy cattle in tropical conditions. High urine-N inputs at Atlántico site did not result in high N2O emissions suggesting that the default Tier 1 emission factor (EF) which is based on N inputs would have overestimated urine-based N2O emissions in Atlántico. Comparing previous studies conducted in Colombia, we observed inter-regional differences by urine-based N2O emissions. This observation suggests that to increase certainty in estimating urine-based N2O emissions, Colombia needs to move toward more region-specific Tier 2 EF and reduce its dependence on the default IPCC Tier 1 EF. In addition, the adoption of Tier 2 EF in the cattle sector will facilitate accounting for the effect of animal diets on N2O inventories.

scholarly journals Relationship between Temperament and Stage of Lactation, Productivity and Milk Composition of Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1840
Author(s):  
Ramūnas Antanaitis ◽  
Vida Juozaitienė ◽  
Vesta Jonike ◽  
Vytenis Čukauskas ◽  
Danguolė Urbšienė ◽  
...  
Keyword(s):  
Milk Fat ◽  
Genetic Correlations ◽  
Subclinical Mastitis ◽  
Milk Composition ◽  
Cow Milk ◽  
Phenotypic Correlation ◽  
Protein Ratio ◽  
Stage Of Lactation ◽  
Milk Urea ◽  
Milk Performance

The aim of this study was to assess the relationship between temperament and milk performance in cows at different stages of lactation, describing their productivity, metabolic status and resistance to mastitis. This study showed that with increasing lactation, cows’ temperament indicators decreased (p < 0.001) and they became calmer. The highest temperament score on a five-point scale was found in cows between 45 and 100 days of lactation. In the group of pregnant cows, we found more cows (p = 0.005) with a temperament score of 1–2 compared with non-pregnant cows A normal temperament was usually detected in cows with lactose levels in milk of 4.60% or more and when the somatic cell count (SCC) values in cow milk were <100,000/mL and 100,000–200,000/mL, with a milk fat-to-protein ratio of 1.2. A larger number of more sensitive and highly aggressive cows was detected at a low milk urea level. In contrast to a positive phenotypic correlation (p < 0.05), this study showed a negative genetic correlation between the temperament of cows and milk yield (p < 0.001). Positive genetic correlations between temperament scores and milk somatic cells (p < 0.001) and milk fat-to-protein ratio (p < 0.05) were found to indicate a lower genetic predisposition in cows with a calmer temperament to subclinical mastitis and ketosis. On the other hand, the heritability of temperament (h2 = 0.044–0.100) showed that only a small part of the phenotypic changes in this indicator is associated with genetic factors.

scholarly journals PSI-3 Effects of housing cow-calf pairs in drylots vs pasture during the summer on cow performance

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 228-228
Author(s):  
Keiffer Sexton ◽  
Megan Myerscough ◽  
Wesley Chapple ◽  
William T Meteer ◽  
Keela Trennepohl ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Binary Data ◽  
Pregnancy Rates ◽  
Corn Silage ◽  
Fat Percentage ◽  
Beef Cow ◽  
Dried Distillers Grains ◽  
Milk Urea ◽  
Cow Performance

Abstract The objective was to evaluate the effects of maintaining cows in drylots vs pasture on beef cow performance. Spring-calving, Simmental × Angus cows (n = 108; 84 ± 11 d postpartum) were stratified by age, BW, BCS, calving date, and sex of calves and allotted to 6 groups. Groups were randomly assigned to 1 of 2 treatments for 110 d: drylot (DL) or pasture (PAST). Drylot cows were limit-fed a ration consisting of corn silage, dried distillers grains, hay, corn stalks, corn, and soybean hulls to meet protein and energy requirements. Cows on pasture were rotationally grazed and offered free-choice mineral. On d 0, cows were artificially inseminated (AI). On d 0, 53, and 110 (weaning), cow BW and BCS were measured. On d 54 milk production was estimated using the weigh-suckle-weigh technique. Data were analyzed using the MIXED procedure of SAS. All binary data were analyzed using the GLIMMIX procedure of SAS. On d 0 and 53, cow BW did not differ (P ≥ 0.73) between DL and PAST. On d 110, DL cows had 74 kg greater (P &lt; 0.01) BW than PAST cows. The BCS did not differ (P ≥ 0.66) between treatments. There was no difference in milk production (P = 0.93); however, drylot cows tended (P = 0.10) to have reduced milk fat percentage and had reduced (P &lt; 0.01) milk urea nitrogen. There was no difference (P ≥ 0.34) in AI or overall pregnancy rates between treatments. There was no difference in foot angle or claw set (P ≥ 0.17) of cows at any time point. There was also no difference (P = 0.17) in foot treatments between DL (39%) and PAST (4%). Housing cows in drylots compared to pasture increased BW, but did not affect BCS, milk production, and pregnancy rates.

scholarly journals Vicilin and legumin storage proteins are abundant in water and alkali soluble protein fractions of glandless cottonseed

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhongqi He ◽  
Christopher P. Mattison ◽  
Dunhua Zhang ◽  
Casey C. Grimm
Keyword(s):  
Mass Spectrometry ◽  
Soluble Protein ◽  
Extraction Efficiency ◽  
Storage Proteins ◽  
Protein Composition ◽  
Protein Fractions ◽  
Peptide Fragments ◽  
2S Albumin ◽  
Cottonseed Protein ◽  
Glandless Cottonseed

AbstractIn this work, we sequentially extracted water (CSPw)- and alkali (CSPa)-soluble protein fractions from glandless cottonseed. SDS-Gel electrophoresis separated CSPw and CSPa to 8 and 14 dominant polypeptide bands (110–10 kDa), respectively. Liquid chromatography-electrospray ionization-tandem mass spectrometry identified peptide fragments from 336 proteins. While the majority of peptides were identified as belonging to vicilin and legumin storage proteins, peptides from other functional and uncharacterized proteins were also detected. Based on the types (unique peptide count) and relative abundance (normalized total ion current) of the polypeptides detected by mass spectrometry, we found lower levels (abundance) and types of legumin isoforms, but higher levels and more fragments of vicilin-like antimicrobial peptides in glandless samples, compared to glanded samples. Differences in peptide fragment patterns of 2S albumin and oleosin were also observed between glandless and glanded protein samples. These differences might be due to the higher extraction recovery of proteins from glandless cottonseed as proteins from glanded cottonseed tend to be associated with gossypol, reducing extraction efficiency. This work enriches the fundamental knowledge of glandless cottonseed protein composition. For practical considerations, this peptide information will be helpful to allow better understanding of the functional and physicochemical properties of glandless cottonseed protein, and improving the potential for food or feed applications.

scholarly journals Comparative Analysis of Electrophoretic Profile of Major Proteins of Milk from Alpine and Carpathian Goats

2017 ◽  
Vol 74 (1) ◽  
pp. 20 ◽  
Author(s):  
Alina NASALEAN ◽  
Laurentiu OGNEAN ◽  
Sergiu MUNTEAN ◽  
Stefana BALICI ◽  
Horea MATEI
Keyword(s):  
Protein Profile ◽  
Milk Proteins ◽  
Goat Milk ◽  
Protein Composition ◽  
Raw Milk ◽  
Biologically Active ◽  
Protein Fractions ◽  
Animal Nutrition ◽  
Sds Page ◽  
Percentage Data

The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and biologically active values. The proposal is to comparatively analyse the protein profile of milk. The milk was collected from two different breeds: French Alpine and Romanian Carpathian. During March and April 2016 there were collected samples of raw milk in hygienic and sanitation conditions. There were two lots: first lot has 10 Carpathian goats and the second lot has 10 Alpine goats. The protein composition of goat milk was established with SDS-PAGE, after the evaluation of the total proteins’ concentration with the Bradford method. The quantitative and percentage data obtained with electrophoresis revealed few differences between those 8 identified protein fractions. Between those two lots, regarding the levels of β-CN, k-CN and β-lactoglobulines there were significant differences. The other protein fractions have values almost identical. Statistical analysis of obtained data shaped the differences in the protein profile at those two breeds. Based on those differences it is to note the superior potential of the Alpine breed regarding the content in biologically active milk proteins. Regarding the obtained data, this study brings new contributions for the evaluation and analysis of protein profile as a nutritive and biologically active component of goat milk, confirming its character as a functional aliment.

Occurrence of milk proteins in the urine of cows during an extended milking interval

1967 ◽  
Vol 34 (1) ◽  
pp. 27-30 ◽  
Author(s):  
R. L. J. Lyster ◽  
J. V. Wheelock
Keyword(s):  
Molecular Weight ◽  
Milk Proteins ◽  
Immunological Methods ◽  
Factors Affecting ◽  
Β Lactoglobulin

SummaryImmunological methods have been used to test samples of urine from 5 cows for the presence of milk proteins. None could be detected when the cows were milked twice daily at the usual intervals, but during an extended milking interval α-lactalbumin was found in the urine of all 5 cows and β-lactoglobulin in the urine of 2 cows. The urine of one cow during and after a milking interval of 39 h contained 1·63 g α-lactalbumin, 1·12 g β-lactoglobulin and a small amount of casein. One of the factors affecting the transfer of these milk constituents from the udder to the urine appears to be their molecular weight.

scholarly journals Investigation of Non-Genetics Factors Affecting Milk-Fat Content and Milk-Fat Percentage Curves' Parameters In Azeri and Khuzestani Buffalo Breeds

2019 ◽  
Vol 10 (26) ◽  
pp. 113-121
Author(s):  
Karim Hasanpur ◽  
Seyad Abbas Rafat ◽  
Arash Javanmard ◽  
Davood Kianzad ◽  
◽  
...  
Keyword(s):  
Milk Fat ◽  
Fat Content ◽  
Fat Percentage ◽  
Factors Affecting
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