scholarly journals Efficient Expression of Xylanase by Codon Optimization and Its Effects on the Growth Performance and Carcass Characteristics of Broiler

Animals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 65 ◽  
Author(s):  
Hong Hu ◽  
Sifa Dai ◽  
Aiyou Wen ◽  
Xi Bai
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Codon Optimization ◽  
Body Weight Gain ◽  
Carcass Characteristics ◽  
Expression Level ◽  
Control Group ◽  
Muscle Weight ◽  
Final Weight

The aim of the present study was to improve the expression level of Trichoderma reesei xylanase (XynB) in Pichia pastoris through a codon optimization strategy and evaluate its effects on the growth performance and carcass characteristics of broiler. According to the codon bias of Pichia genome, the XynB gene from T. reesei was optimized and synthesized by whole gene assembly to improve its expression level in P. pastoris. Approximately 180 target mutations were successfully introduced into natural XynB. The maximum activity of xylanase (optiXynB) secreted by P. pastoris pPICZaA-optiXynB was 1299 U/mL after 96 h induction. Purified recombinant optiXynB had the molecular weight of 24 kDa. The optiXynB presented highest activity in pH 5.0 and 50 °C. The recombinase was highly specific towards birchwood xylan, beechwood xylan, and oat-spelt xylan. In the broiler experiment, a total of 200 Arbor Acre broilers (one day old) were randomly allocated into four groups fed with basal diets containing 0 (control group), 500, 1000, and 1500 IU/kg optiXynB. Dietary 1000 and 1500 IU/kg optiXynB significantly increased (p < 0.05) final weight and body weight gain; dietary 500, 1000, and 1500 IU/kg optiXynB significantly increased (p < 0.05) pre-evisceration weight, dressed percentage, and eviscerated weight compared with the control group. Inclusion of optiXynB in broiler diets linearly increased final weight, body weight gain, breast muscle weight and leg muscle weight, but linearly decreased feed conversion rate (p < 0.05). Furthermore, inclusion of optiXynB in broiler diets linearly and quadratically increased pre-evisceration weight, dressed percentage, and eviscerated weight (p < 0.05). The recombinant optiXynB from P. pastoris pPICZaA-optiXynB was beneficial in improving growth performance and carcass characteristics of broilers.

scholarly journals Are the energy matrix values of the different feed additives in broiler chicken diets could be summed?

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Abdallah E. Metwally ◽  
Ahmed A. A. Abdel-Wareth ◽  
Ahmed A. Saleh ◽  
Shimaa A. Amer
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Economic Efficiency ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Control Group ◽  
Energy Matrix ◽  
Degrading Enzymes

Abstract Background The aim of this study is to investigate whether the energy matrix values of the nonstarch polysaccharide- (NSP-) degrading enzymes, bioemulsifier (LYSOFORTE®), guanidinoacetic acid (CreAMINO®), or their combinations could be summed. The effects of these additives on the growth performance, carcass traits, and economic value of the broiler chicken diets were evaluated. A total of 525-one-day-old Ross chicks with an initial body weight of 42.96 ± 0.87 g were haphazardly allocated into seven groups with five replicates. The seven experimental treatments are as follows: (1) basal diet with no additives (breeder recommendation), which is the control group, (2) basal diet minus 100 kcal/kg supplemented with 0.02% NSP-degrading enzymes (NSP), (3) basal diet minus 50 kcal/kg supplemented with 0.025% emulsifier (LYSOFORTE®), (4) basal diet minus 50 kcal/kg supplemented with 0.06% guanidinoacetic acid (CreAMINO®), (5) basal diet minus 150 kcal/kg supplemented with a mixture of NSP and LYSOFORTE® (NSPL), (6) basal diet minus 100 kcal/kg supplemented with a mixture of NSP and CreAMINO® (NSPC), and (7) basal diet minus 200 kcal/kg supplemented with a mixture of NSP, LYSO, and CreAMINO® (NSPLC). The experiment lasted for 35 days. Results It was found that the final body weight, body weight gain, and relative growth rate were significantly higher in birds fed diets supplemented with NSPL, NSPC, CreAMINO, and LYSO with the reduced energy matrix value. The overall feed conversion ratio was significantly improved due to the supplementation of NSPC, CreAMINO, NSPL, and LYSO with the reduced energy matrix value compared to the control group. Moreover, no significant effect on the carcass criteria was observed by the different treatments. As a result of the dietary supplementation with NSPL, NSPC, CreAMINO®, and LYSO with the reduced energy matrix value, the net profit, total return, economic efficiency, and performance index were increased and the cost of feed per kg of body weight gain was decreased. Conclusion The energy matrix value of NSPL, NSPC, CreAMINO®, and LYSOFORTE could be established in the diets of broiler chickens to improve the growth performance and economic efficiency.

scholarly journals Effects of Neem and Nishyinda leaves supplementation on growth performance and gut biosis in broiler

2020 ◽  
Vol 6 (2) ◽  
pp. 168-175
Author(s):  
Atikur Rahaman ◽  
Md Bazlar Rashid ◽  
Md Mahmudul Hasan ◽  
Rakibul Islam ◽  
Fahima Binthe Aziz ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Health Hazard ◽  
Feed Consumption ◽  
Control Group ◽  
Broiler Chicks ◽  
Neem Leaves ◽  
Group D

This study was conducted under the Department of Physiology and Pharmacology to determine the effects of Neem and Nishyinda leaves supplementation on growth performance and GUT biosis in broilers. A total of 40 day old broiler chicks were divided into four groups A, B, C and D. Group A was considered as control, fed only with commercial broiler ration. Group B supplemented with formulation of 2 gm grinded Neem leaves, Group C with 2 gm grinded Nishyinda leaves and Group D with 1 gm grinded Neem leaves plus 1 gm grinded Nishyinda leaves per liter of water respectively. Observations were recorded for live body weight, weight gain, feed consumption, feed efficiency, hemato-biochemical parameters and GUT biosis specially the parasite of birds in six weeks. Body weights were increased significantly (p<0.05) in all treated groups in respect to the control and highest was recorded in combine Neem plus Nishyinda supplemented groups (Group D). No significant (p>0.05) differences were observed among the groups for PCV and TEC values. Hb content increased and ESR decreased significantly (p<0.05) with combine Neem plus Nishyinda supplemented group (Group D). The serum SGOT and SGPT levels were also decreased with the same kind of supplementation. In treatment group there was significantly decreased of parasitic eggs, but in case of control group no decrease of parasitic eggs. There was no significant pathological change in any internal organs of the broiler of treated groups. Best result was found in the group D. The present study reveals that combine supplementation of Neem plus Nishyinda gives better result over other groups in respect to body weight gain, feed conversion ratio, hemato-biochemical parameter and profitability without making any health hazard of the broilers. Asian J. Med. Biol. Res. June 2020, 6(2): 168-175

scholarly journals Effects of mushroom waster medium and stalk residues on the growth performance and oxidative status in broilers

2021 ◽  
Vol 34 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Y. C. Hsieh ◽  
W. C. Lin ◽  
W. Y. Chuang ◽  
M. H. Chen ◽  
S. C. Chang ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Feed Additives ◽  
Control Group ◽  
Heme Oxygenase 1 ◽  
Antioxidant Genes ◽  
Significant Difference ◽  
Functional Components

Objective: The study developed mushroom stalk residues as feed additives in the broiler diet for improving the growth performance and immunity of broilers as well as to increase the value of mushroom stalk residues.Methods: In total, 300 ROSS 308 broilers were randomly allocated into fifteen pens with five dietary treatments: i) control, basal diet; ii) CMWM, supplemented with 1% <i>Cordyceps militaris</i> waster medium (CM); iii) CMPE, supplemented with 0.5% CM+0.5% <i>Pleurotus eryngii</i> stalk residue (PE); iv) CMPS, supplemented with 0.5% CM+0.5% <i>Pleurotus sajorcaju</i> stalk residue (PS); v) CMFV, supplemented with 0.5% CM+0.5% <i>Fammulina velutipes</i> stalk residue (FV).Results: The chemical analysis results showed that CM extracts, PE extracts, PS extracts, and FV extracts contain functional components such as polysaccharides and phenols and have both 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate scavenging and Ferrous scavenging capacities. The group CMWM saw increased body weight gain and feed conversion rate and the promotion of jejunum villus growth, but there is no significant difference in the intestinal bacteria phase. Antioxidant genes in the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)- antioxidant responsive element pathway among the groups are significantly higher than that of the control group, especially in group CMWM.Conclusion: The mushroom stalk residues have antioxidant functional components, can improve the intestinal health and body weight gain of chickens, and can activate the antioxidant pathway of Nrf2 to increase the heme oxygenase-1 expression. The treatment with 1% CM was the most promising as a feed additive.

scholarly journals Effect of Lupine and Amaranth on Growth Efficiency, Health, and Carcass Characteristics and Meat Quality of Market Pigs

2006 ◽  
Vol 75 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Z. Zralý ◽  
B. Písaříková ◽  
M. Trčková ◽  
I. Herzig ◽  
M. Jůzl ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Carcass Characteristics ◽  
Growth Efficiency ◽  
Control Group ◽  
Feed Conversion ◽  
Amaranth Grain ◽  
Experimental Group ◽  
Group 1

The purpose of the present study was to ascertain whether it is possible to substitute animal protein in a pig diet with lupine of Sonet cultivar or amaranth grain or dried surface biomass of amaranth when one type feed ration is used during the entire period of fattening, and to investigate its impact on growth efficiency and health of pigs. Its role in feed conversion, carcass characteristics, meat quality and sensory parameters was analyzed. Four groups of pigs (n = 10, 5 males and 5 females) with the body weight of 24 kg were fed semi-ad libitum for 90 days the following diets: control group (C) - diet containing 3% of fish meal, experimental group (1) - diet containing 5% of non-heat-treated amaranth grain and 5% of dried surface biomass of amaranth, experimental group (2) - diet containing 5% of popped (heattreated) amaranth grain and 5% of dried surface amaranth biomass, experimental group (3) - diet containing 10% of lupine seed meal. Animal protein substitution in diets with amaranth (group 1, 2) or lupine (3) did not result in significant differences (p > 0.05) in average daily body weight gain in comparison with the control group (C - 0.83 and 0.82, 0.80 and 0.79 kg in groups 1, 2 and 3, respectively). The differences in feed conversion were non-significant (2.69 to 2.79 kg/kg of body weight gain). The tested diets did not adversely affect animal metabolism, and significantly lower concentrations (p < 0.05, p < 0.01) of total protein, glucose and triacylglycerol in animals of group C ranged within physiological limits. No significant differences between control and experimental animals were found in carcass characteristics, meat and sensory qualities. Lupine supplementation of the diet positively affected the tenderness and taste of meat.

scholarly journals Efficacy of neem leaf, nishyinda leaf and turmeric rhizome supplementation on the growth performance of broiler chicken

2016 ◽  
Vol 3 (2) ◽  
pp. 331-338
Author(s):  
Nahid Nawrin Sultana ◽  
Soheli Jahan Mou ◽  
Mahbub Mostofa ◽  
Md Abdur Rahman
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Control Group ◽  
Final Body Weight ◽  
Live Body Weight ◽  
Significant Difference ◽  
Group A ◽  
Group B

This experiment was conducted to evaluate the efficacy of Neem (Azadirachta indica) leaf, Nishyinda (Vitex nogundo) leaf and Turmeric rhizome (Curcuma longa) powdered supplementation in drinking water as a growth promoter in broiler chickens. A total of 40 day-old Cobb 40 broiler chicks were purchased from local hatchery (Nourish Poultry & Hatchery Ltd.) and after seven days of acclimatization chicks were randomly divided into two groups, A and B. The group A was kept as a control and not treated. The group B was supplemented with Neem, Nishyinda leaves and Turmeric dried powder with feed and water. Weekly observations were recorded for live body weight gain up to 5th weeks and hematological tests were performed at 7th and 35th day’s age of broiler to search for hematological change between control (A) and treatment (B) groups. The initial body weight of groups A and B on 7th day of this experiment were 130±4.35 gm, respectively and after 35th day of experiment final body weight were 150±47.35 gm and 1600±58.56 gm, respectively; the net body weight gain were 1320±43.79 gm and 1470±54.25 gm, respectively and economics of production were analyzed and found that net profit per broiler was Tk. 17.24 and Tk. 30.00, respectively. The treatment group B was recorded statistically significant (at 1% level) increase for live body weight than that of control group A. The hematological difference, while Hb. estimation does not show significant difference from control group. The results suggest that better growth performance could be achieved in broilers supplemented with Neem, Nishyinda leaves and Turmeric rhizome extract.Res. Agric., Livest. Fish.3(2): 331-338, August 2016

scholarly journals Effects of L-carnitine and iron diet supplementations on growth performance, carcass characteristics and blood metabolites in fattening pigs

2008 ◽  
Vol 18 (1) ◽  
pp. 27 ◽  
Author(s):  
A. PIETRUSZKA ◽  
E. JACYNO ◽  
A. KOLODZIEJ
Keyword(s):  
Body Weight ◽  
Blood Serum ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Carcass Characteristics ◽  
Basal Diet ◽  
Growing Pigs ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Fattening Pigs

The aim of this study was to evaluate the effect of dietary L-carnitine supplementation either with or without extra Fe supplementation from Fe-amino acid (Fe-AA) complex on body weight gain, feed conversion, carcass characteristics and blood metabolite concentrations in fattening pigs. The study was carried out with 75 fatteners (30.100 kg body weight), divided into three groups, of 25 pigs each. The control group was given a basal diet that contained 85 mg/kg of Fe from premix. A L-carnitine group was given a basal diet supplemented with 100 mg/kg of L-carnitine, and a L-carnitine+Fe group was given a basal diet supplemented with 100 mg/kg of L-carnitine and 60 mg/kg of Fe from a Fe-AA complex. The supplement of L-carnitine to the diets did not have any effects on the growth performance and carcass traits. The L-carnitine supplement decreased the concentration of triglycerides (p.0.05), cholesterol (p .0.05) and low-density lipoproteins (p .0.01) in the blood serum of pigs, while it increased (p.0.01) the concentration of high-density lipoproteins. The combination of L-carnitine+Fe increased the growth performance of growing pigs (p .0.05) and the lean percentage and fatless ham weight in carcass and also increased (p .0.05) the Fe content in the blood serum and the longissimus dorsi muscle of pigs. The results suggest the more effective outcomes can be reached when L-carnitine+Fe rather than only L-carnitine is used in diets.;

Growth and carcass characteristics of three lamb genotypes finished on the same level of feeding

2000 ◽  
Vol 70 (1) ◽  
pp. 51-61 ◽  
Author(s):  
L. O. W. McClintont ◽  
A. F. Carson
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Fatty Acid ◽  
Weight Gain ◽  
Polyunsaturated Fatty Acids ◽  
Body Weight Gain ◽  
Saturated Fatty Acids ◽  
Carcass Characteristics ◽  
Live Weight ◽  
Ash Content

AbstractThis study investigated the efficiency of growth and the carcass characteristics of 24 Greyface (Border Leicester × Scottish Blackface), 24 Texel (12 purebred and 12 Texel × Texel-Greyface) and 24 Rouge (12 purebred and 12 Rouge × Rouge-Greyface) lambs finished on the same level of feeding. The efficiency of live-weight gain (kg/MJ) was higher in Greyface compared with Texel lambs (P< 0·01). The efficiency of empty body-weight gain (kg/MJ) was higher in Greyface (P< 0·01) and Rouge (P< 0·05) compared with Texel lambs. The efficiency of carcass gains (kg/MJ) tended to be higher in Greyface and Rouge compared with Texel lambs (P= 0·07). The efficiency of non-carcass component gains (kg/MJ) was also higher in Greyface compared with Texel lambs (P0·05). Carcass water, protein, lipid and ash gains did not vary significantly between the genotypes, however carcass energy gain tended to be higher in Greyface and Rouge compared with Texel lambs (P= 0·08). The relative proportions of water, protein, lipid and ash in carcass gains did not vary significantly between the genotypes. At the end of the experiment carcass water content was higher in Texel compared with Greyface lambs (P< 0·05) and carcass ash content was lower in Texel compared with Greyface (P< 0·01) and Rouge (P< 0·05) lambs. The concentration of saturated fatty acids was higher in Greyface compared with Rouge lambs (P< 0·001) and higher in Rouge compared with Texel lambs (P< 0·05). Monounsaturated fatty acid concentrations were higher in Rouge compared with Greyface lambs (P< 0·05) and higher in Texel compared with Rouge lambs (P< 0·001). Polyunsaturated fatty acid concentrations were higher in Rouge and Texel compared with Greyface lambs (P< 0·01). The ratio of n-6:n-3 fatty acids was lower in Rouge compared with Greyface lambs (P< 0·05).The efficiency of empty body gain was higher in male compared with female lambs (P< 0·05). Carcass water (P< 0·01) and protein (P< 0·05) gains were higher in male lambs. At the end of the experiment male carcasses contained a higher content of water (P< 0·05), protein (P< 0·01) and ash (P= 0·07), and a lower lipid (P< 0·05) and energy (P< 0·001) content. Carcass lipids from male lambs contained a higher concentration of polyunsaturated fatty acids (P< 0·001) and tended to contain a lower concentration of saturated fatty acids (P = 0·06).

scholarly journals Effect of dietary supplementation of seaweed (Ulva lactuca) and Azolla on growth performance, haematological and serum biochemical parameters of Aseel chicken

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Vijayalingam Thavasi Alagan ◽  
Rajesh Nakulan Vatsala ◽  
Ilavarasan Sagadevan ◽  
Vairamuthu Subbiah ◽  
Venkataramanan Ragothaman
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Well Being ◽  
Ulva Lactuca ◽  
Feed Conversion ◽  
Serum Biochemical

Abstract Background An effort was made to assess the effectiveness of dietary supplementation of Ulva lactuca (U. lactuca), a seaweed and Azolla individually and in combination on different physiological parameters of Aseel chicken. A total of 40 Aseel chicks of 8 weeks old were allocated into four groups, C1 (control), T1 (Azolla alone), T2 (seaweed alone) and T3 (seaweed plus Azolla). Aseel chicks in C1 were fed with formulated grower feed alone, birds in T1 had control diet with 5% Azolla, birds in T2 had control diet with 3% U. lactuca and birds in T3 had control diet admixtured with 5% of Azolla and 3% of U. lactuca. Parameters were recorded for a continuous period of 2 months in 15 days interval. Results The growth performance was found to be significant (P ≤ 0.05) during 30 days of feed trials and highly significant (P ≤ 0.01) during 45 and 60 days of treatment. Birds in T3 had a higher body weight gain, shank length and feed conversion efficiency followed with T1 and T2 compared to C1. PCV, RBC, WBC and heterophil counts were not influenced (P ≥ 0.05) by dietary treatments. Uric acid, creatinine, AST, glucose, triglycerides and magnesium levels revealed a high significant (P ≤ 0.01) variation, and cholesterol level showed significant (P ≤ 0.05) changes during 30th and 60th day of feed trials between the control and treatment groups. Total protein, globulin, phosphorus and electrolytes like Na, K and Cl levels were not significant (P ≥ 0.05) during 30th day and were highly significant (P ≤ 0.01) during the 60th day of the trial. Conclusions It could be noted that inclusion of U. lactuca and Azolla as feed supplement in grower chicken had a better body weight gain when given in combination (U. lactuca and Azolla) rather than supplemented with the sole entity. Based on the haematological and serum biochemical analysis, the supplementation of U. lactuca and Azolla at the levels included in this trial did not pose any threat to the physiological well-being of Aseel chicken.

Growth performance, nutrient digestibility, carcass and economic characteristics of turkey poults fed millet-based diets as a replacement for maize

2021 ◽  
Vol 41 (1) ◽  
pp. 91-99
Author(s):  
A. A. Malik ◽  
M. J. Ibrahim ◽  
Y. S. Kudu ◽  
O. P. Jibogun ◽  
Y. J. Adeniyi ◽  
...  
Keyword(s):  
Weight Gain ◽  
Growth Performance ◽  
Lower Cost ◽  
Body Weight Gain ◽  
Carcass Characteristics ◽  
Live Weight ◽  
Nutrient Digestibility ◽  
Feeding Trial ◽  
Turkey Poults ◽  
Dietary Treatments

A nine (9) weeks feeding trial was conducted on seventy two (72) day-old Indigenous turkey poults raised on deep litter to determine their growth performance, carcass and economic characteristics when fed three dietary treatments (T1, T2, and T3, containing 0%, 25% and 50% replacement of maize with millet respectively. The birds were divided into three replicates of 8 birds per replicate; making a total of 24 birds per treatment. At the end of the & week, 4 birds per replicate were transferred to specially-designed metabolism cages for a digestibility study involving the collection of faecal droppings; while at the end of the week of the experiments, two birds per replicate were randomly selected and slaughtered to determine their carcass characteristics. Turkey poults fed millet at 25 %replacement showed significantly (p<0.05) higher body weight gain, lower cost of feed per kg live weight gain and higher revenue generated per bird; but there were no significant (p>0.05) differences in the performance of birds on 0% and 50 % replacements for those parameters. Also, there were no significant (p>0.05) differences in feed intake and mortality across the treatments. Similarly, there were no significant (p>0.05) differences in nutrient digestibilities and carcass characteristics of the birds among the various dietary treatments. Therefore, it can be concluded hat millet cam be used to replace maize up to S0 % in the diets of turkey poults at the starter phase with no detrimental effects on their performance

The effects of oil palm leaf meal-based diets on the growth performance and haematological indices of Red Sokoto goats

2021 ◽  
Vol 17 (1) ◽  
pp. 27-34
Author(s):  
A.I. Ukanwoko ◽  
C.T. Alfred
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Feed Intake ◽  
Oil Palm ◽  
Body Weight Gain ◽  
Final Body Weight ◽  
Leaf Meal ◽  
Haematological Indices ◽  
Palm Leaf

An experiment was carried out to determine the growth performance and haematological indices of Red Sokoto goats placed on oil palm leaf meal (OPLM)-wheat offal based diet. Six goats aged 12-18 months, weighing 15 – 20kg were randomly assigned to three treatments, comprising two animals each in a Completely Randomized Design. Each animal received 1kg of the diet daily for 56 days and weighed weekly. Results showed that final body weight was significant (P<0.05) with goats on T3 having the highest (21.50kg). The feed intake, body weight gain and Feed Conversion Ratio (FCR) were significantly different (P<0.05). T3 had the highest feed intake (305.35g/d), highest body weight gain (90.84g/d) and the best FCR (3.36). Significant differences (P <0.05) were observed in the Red Blood Cell (RBC), Packed Cell Volume (PCV) and Haemoglobin (Hb) counts, ranging between 3.12 - 4.20x106ml; 23.00 -30.50% and 7.70 - 10.15g/dl respectively with goats on T3 having the highest.  The inclusion of OPLM enhanced body weight gain, FCR, RBC, PCV, and Hb counts of the goats. Therefore the oil palm leaf meal diets are recommended for better goat production. Key word: Growth performance; Oil Palm Leaf meal., Blood profile., Creatinine., Red Sokoto Goat.

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