scholarly journals Monensin Alters the Functional and Metabolomic Profile of Rumen Microbiota in Beef Cattle

Animals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 211 ◽  
Author(s):  
Ibukun Ogunade ◽  
Hank Schweickart ◽  
Kenneth Andries ◽  
Jerusha Lay ◽  
James Adeyemi
Keyword(s):  
Linoleic Acid ◽  
Amino Acid ◽  
Beef Cattle ◽  
Relative Abundance ◽  
Rumen Fluid ◽  
Differentially Expressed ◽  
Beef Steers ◽  
Rumen Microbiota ◽  
Rumen Microbiome ◽  
Clostridium Symbiosum

To identify differences in rumen function as a result of feeding monensin to beef cattle, rumen fluid metagenomics and metabolomics analyses were used to evaluate the functional attributes and metabolites of rumen microbiota in beef steers fed no or 200 mg/d of monensin. Eight rumen-fistulated steers were used in the study for a period of 53 days. Rumen fluid samples were collected on the last day of the experiment. Monensin increased the relative abundance of Selenomonas sp. ND2010, Prevotella dentalis, Hallella seregens, Parabacteroides distasonis, Propionispira raffinosivorans, and Prevotella brevis, but reduced the relative abundance of Robinsoniella sp. KNHs210, Butyrivibrio proteoclasticus, Clostridium botulinum, Clostridium symbiosum, Burkholderia sp. LMG29324, and Clostridium butyricum. Monensin increased the relative abundance of functional genes involved in amino acid metabolism and lipid metabolism. A total of 245 metabolites were identified. Thirty-one metabolites were found to be differentially expressed. Pathway analysis of the differentially expressed metabolites revealed upregulated metabolic pathways associated with metabolism of linoleic acid and some amino acids. These findings confirm that monensin affects rumen fermentation of forage-fed beef cattle by modulating the rumen microbiome, and by reducing amino acid degradation and biohydrogenation of linoleic acid in the rumen.

scholarly journals PSIX-5 Effects of live yeast on functional attributes of rumen microbiota in beef cattle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 394-395
Author(s):  
Ibukun M Ogunade ◽  
Jerusha Lay ◽  
Kenneth Andries
Keyword(s):  
Beef Cattle ◽  
Relative Abundance ◽  
Rumen Fluid ◽  
Basal Diet ◽  
Glycoside Hydrolases ◽  
Carbohydrate Binding ◽  
Illumina Hiseq ◽  
Rumen Microbiota ◽  
Carbohydrate Binding Modules ◽  
Live Yeast

Abstract This study applied whole-metagenomics shotgun sequencing to evaluate the effects of live yeast supplementation on functional potential of rumen microbiota in beef cattle. Eight rumen-cannulated Holstein steers were assigned randomly to 1 of 2 treatments in cross-over design with two 25-d experimental periods and a 10-d wash-out between the two periods. The steers were housed in individual pens and were fed 50% concentrate and 50% red clover hay ad libitum. Dietary treatments were (1) control (CON; basal diet) and (2) yeast (YEA; basal diet plus 15 g/d of yeast product; PMI, Arden Hills, MN, USA). Rumen fluid samples were collected at 3, 6 and 9 h after feeding on the last day of each period. Sequencing was done on an Illumina HiSeq 2500. Differences in the relative abundance of taxa at the species level were analyzed using the GLIMMIX procedure of SAS using a model that included the effects of treatment, period, and their interaction. Dietary yeast supplementation increased (P < 0.05) the relative abundance of carbohydrate-fermenting bacteria, such as Ruminococcus albus, R. champanellensis, R. bromii, and R. obeum, and lactate-utilizing bacteria, such as Megasphera elsdenii, Desulfovibrio desulfuricans, and D. vulgaris. A total of 154 differentially abundant microbial genes (DAGs) were obtained (false discovery rate < 0.01). Kyoto Encyclopedia of Genes and Genomes annotation analysis of the DAGs revealed that pathways involving amino sugar and nucleotide sugar metabolism, oxidative phosphorylation, pantothenate and CoA biosynthesis, beta-alanine metabolism, and polyketide sugar unit biosynthesis were enriched in steers fed YEA. Annotation of the DAGs in carbohydrate-active enzymes database revealed that genes coding for enzymes belonging to glycoside hydrolases, glycosyltransferases, and carbohydrate binding modules were enriched in steers fed YEA. These findings confirm the efficacy of live S. cerevisiae product at reducing redox potential and increasing cellulolytic and lactate-utilizing activities in the rumen.

scholarly journals PSVI-22 Rumen microbiome of beef cattle is modulated by backgrounding systems

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 220-220
Author(s):  
Bobwealth O Omontese ◽  
Ashok K Sharma ◽  
Jason Langlie ◽  
Joe Armstrong ◽  
Alfredo DiCostanzo ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Developmental Stages ◽  
Production Systems ◽  
Rumen Fluid ◽  
Illumina Miseq ◽  
High Energy ◽  
Shannon Index ◽  
Bacterial Gene ◽  
Beef Calves ◽  
Rumen Microbiome

Abstract Backgrounding (BKG) segment in beef production systems is characterized by utilization of different forages which affect growth performance and carcass characteristics. However, it is unclear how BKG systems impact rumen microbiome. We investigated rumen microbiome dynamics of beef calves under different BKG systems. At weaning, Angus and Angus x Simmental beef calves (n = 38) were stratified by age, weight, and sex in a completely randomized design into 1 of 3 BKG treatments for 55 d: 1) perennial pasture (PP; quackgrass, orchardgrass; smooth bromegrass, red clover, and alfalfa); 2) summer annual cover crop (CC; cereal oats, purple top turnips, hunter forage brassica, and graza forage radish); and 3) dry lot (DL; haylage, corn, and DDGS). After BKG, all calves were assigned to a high energy ration in a feedlot. Rumen sample was collected via esophageal tubing at weaning, BKG and feedlot. A total of 190 rumen fluid samples were used to sequence the hypervariable V4 region of the 16S rRNA bacterial gene on an Illumina MiSeq platform. The results showed that BKG systems largely influenced rumen bacterial communities. Specifically, microbiome composition and diversity were not different at weaning, diverged significantly during BKG (Shannon index, Bray Curtis distance metrics; P < 0.001) and homogenized during feedlot. During the BKG segment, the bacterial genera Agrobacterium, Coprococcus, and Ruminococcus were dominant in CC whereas Fibrobacteraceae and Mycoplasmataceae was most dominant in DL. Moreover, rumen microbiome patterns of CC and DL calves showed increased plasticity in early stages of development but not during feedlot with PP showing fewer changes over time. These results indicate that BKG systems significantly modulate the rumen microbiome of beef cattle and, underscore the importance of early developmental stages as potential targets for feeding interventions that can impact the animal microbiome to enhance animal performance.

scholarly journals Combined signature of rumen microbiome and metabolome in dairy cows with different feed intake levels

2020 ◽  
Vol 98 (3) ◽  
Author(s):  
Yeqing Q Li ◽  
Yumeng M Xi ◽  
Zedong D Wang ◽  
Hanfang F Zeng ◽  
Zhaoyu Han
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Linolenic Acid ◽  
Relative Abundance ◽  
Feed Intake ◽  
Metabolic Pathways ◽  
Acid Metabolism ◽  
Ruminal Fermentation ◽  
Alpha Linolenic Acid ◽  
Rumen Microbiome

Abstract Feed intake is a major factor in maintaining the balance between ruminal fermentation and the microbial community of dairy cows. To explore the relationship among feed intake, microbial metabolism, and ruminal fermentation, we examined the combined signatures of the microbiome and metabolome in dairy cows with different feed intake levels. Eighteen dairy cows were allocated to high feed intake (HFI), medium feed intake (MFI), and low feed intake (LFI) groups according to their average daily feed intake. 16S rDNA sequencing results revealed that the relative abundance of Firmicutes in the HFI group was significantly higher than that in the MFI and LFI groups (P < 0.05). The ratio of Bacteroidetes to Firmicutes was significantly lower in the HFI group than in the MFI and LFI groups (P < 0.05). The relative abundance of Lachnospiraceae_unclassified, Veillonellaceae_unclassified, and Saccharofermentants was significantly higher in the HFI group than in the LFI and MFI groups (P < 0.05). The relative abundance of Erysipelotrichaceae_unclassified and Butyrivibrio was significantly higher in the HFI group than in the MFI and LFI groups (P < 0.05). Ultra high performance liquid chromatography-mass spectrometry revealed five key pathways, including the linoleic acid metabolism pathway, alpha-linolenic acid metabolism, arginine and proline metabolism, glutathione metabolism, and valine, leucine, and isoleucine biosynthesis, which are closely related to energy and amino acid metabolism. Linoleic acid, glutamate, alpha-linolenic acid, l-methionine, and l-valine levels were significantly lower in the HFI group than in the MFI and LFI groups (q < 0.05), while the relative content of glutamate was significantly lower in the MFI group than in the LFI group (q < 0.05). Stearic acid content was significantly higher in the HFI group than in the LFI group (q < 0.05). Our findings provide insight into the rumen microbiome of dairy cows with different feed intake and the metabolic pathways closely associated with feed intake in early-lactating cows. The candidates involved in these metabolic pathways may be useful for identifying variations in feed intake. The signatures of the rumen microbiome and metabolome in dairy cows may help make decisions regarding feeding.

scholarly journals Metatranscriptomic Analysis of Sub-Acute Ruminal Acidosis in Beef Cattle

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 232 ◽  
Author(s):  
Ibukun Ogunade ◽  
Andres Pech-Cervantes ◽  
Hank Schweickart
Keyword(s):  
Beef Cattle ◽  
Rumen Fluid ◽  
Control Diet ◽  
Bacterial Biofilm ◽  
Ruminal Bacteria ◽  
Subacute Ruminal Acidosis ◽  
Rumen Microbiota ◽  
Ruminal Acidosis ◽  
Rrna Sequencing ◽  
Illumina Hiseq4000

Subacute ruminal acidosis (SARA) is a metabolic disease of ruminants characterized by low pH, with significant impacts on rumen microbial activity, and animal productivity and health. Microbial changes during subacute ruminal acidosis have previously been analyzed using quantitative PCR and 16S rRNA sequencing, which do not reveal the actual activity of the rumen microbial population. Here, we report the functional activity of the rumen microbiota during subacute ruminal acidosis. Eight rumen-cannulated Holstein steers were assigned randomly to acidosis-inducing or control diet. Rumen fluid samples were taken at 0, 3, 6, and 9 h relative to feeding from both treatments on the challenge day. A metatranscriptome library was prepared from RNA extracted from the samples and the sequencing of the metatranscriptome library was performed on Illumina HiSeq4000 following a 2 × 150 bp index run. Cellulolytic ruminal bacteria including Fibrobacter succinogenes, Ruminococcus albus, and R. bicirculans were reduced by an induced acidotic challenge. Up to 68 functional genes were differentially expressed between the two treatments. Genes mapped to carbohydrate, amino acid, energy, vitamin and co-factor metabolism pathways, and bacterial biofilm formation pathways were enriched in beef cattle challenged with sub-acute acidosis. This study reveals transcriptionally active taxa and metabolic pathways of rumen microbiota during induced acidotic challenge.

scholarly journals PSIX-4 Metatranscriptomic analysis of sub-acute ruminal acidosis in beef cattle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 394-394
Author(s):  
Ibukun M Ogunade ◽  
Andres Pech Cervantes
Keyword(s):  
Discriminant Analysis ◽  
Beef Cattle ◽  
Linear Discriminant Analysis ◽  
Rumen Fluid ◽  
Control Diet ◽  
Bacterial Biofilm ◽  
Functional Genes ◽  
Linear Discriminant ◽  
Rumen Microbiota ◽  
Ruminal Acidosis

Abstract This study evaluated the functional activity of the rumen microbiota during subacute ruminal acidosis (SARA) using metatranscriptomics. Eight rumen-cannulated Holstein steers were assigned randomly to control diet (CON) or acidosis-inducing diet (CHA) for 20 d. Sub-acute ruminal acidosis was induced on d 18; feed was restricted to 50% of ad libitum intake for CHA group on day 17. On day 18, ground corn grain, equivalent to 25% of the mean dry matter intake (DMI) of each steer in CHA treatment was administered directly in the rumen prior to feeding. Thereafter, rumen fluid samples were taken at 0, 3, 6, and 9 h relative to feeding from both treatments. Metatranscriptome library was prepared from RNA extracted from the samples and sequencing of metatranscriptome library was done on Illumina HiSeq4000 following a 2 x 150bp index run. Linear discriminant analysis (LDA) effect size comparisons were made between CON and CHA groups. Alpha level of 0.05 was used for both the Kruskal–Wallis and pairwise Wilcoxon tests. Linear discriminant analysis scores greater than 2.0 were used for taxonomy and 1.0 for functional genes. Additionally, functional genes enriched in both treatments were mapped to the KEGG reference metabolism pathway. Cellulolytic rumen bacteria including Fibriobacter succinogenes, Ruminococcus albus, and R. bicirculans were reduced (LDA > 2.0, P < 0.05) during SARA. Up to 68 functional genes were differentially expressed between the two treatments (LDA > 1.0, P < 0.05). Genes mapped to carbohydrate, amino acid, energy, vitamin and co-factor metabolism pathways, and bacterial biofilm formation pathways (Pseudomonas aeruginosa, Escherichia coli and Vibrio cholera) were enriched in acidotic-challenged beef cattle. This study explains the pathogenicity of SARA and enhances our understanding of the response of rumen microbiome to SARA by revealing transcriptionally active taxa and metabolic pathways of rumen microbiota.

Effect of mash or pelleted supplements containing crab meal on intake and weight gains of beef cattle

1996 ◽  
Vol 76 (1) ◽  
pp. 95-103 ◽  
Author(s):  
J. W. G. Nicholson ◽  
R. E. McQueen ◽  
J. G. Allen ◽  
R. S. Bush
Keyword(s):  
Beef Cattle ◽  
Rumen Fluid ◽  
Beef Steers ◽  
Fiber Digestion ◽  
Weight Gains ◽  
Alfalfa Meal ◽  
Sheep Rumen ◽  
Fourth Experiment ◽  
Cattle Performance

Yearling beef steers showed a progressive decrease in rate of gain as crab meal replaced 33, 66 and 100% of supplemented soybean meal. The crab meal was highly resistant to degradation in sacco but was well digested in the whole tract of sheep. Rumen fluid from sheep fed crab meal was as effective for fiber digestion in vitro as that from sheep fed a similar diet without crab meal. It was concluded that the lower feed intake observed with cattle fed crab meal was not due to inhibition of fiber digestion. In a second experiment, there was no improvement in cattle performance when the crab meal was treated with the antioxidant ethoxyquin. Oxidation of crab meal that could lead to rancidity does not appear to be a problem, as adding ethoxyquin did not affect cattle performance. In a third experiment, pelleting a crab meal–alfalfa meal supplement increased intake from 1.22 kg d−1 to 1.64 kg d−1 (P < 0.01), and pelleting a crab meal–barley supplement increased intake from 1.55 kg d−1 to 1.80 kg d−1 (P < 0.05). In a fourth experiment, pelleting the crab meal supplement again largely overcame the depressing effects of crab meal on intake and weight gains. It was concluded that crab meal is a useful supplement for young cattle, provided they can be induced to consume it. Pelleting the crab meal supplement is one way of overcoming the lower intake and weight gains associated with feeding it as a mash. Key words: Crab meal, intake, digestibility, beef cattle, pelleting

scholarly journals Whole-Plant Corn Silage Improves Rumen Fermentation and Growth Performance of Beef Cattle by Altering Rumen Microbiota

2021 ◽  
Author(s):  
Yalei Cui ◽  
Hua Liu ◽  
Zimin Gao ◽  
Junying Xu ◽  
Boshuai Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Beef Cattle ◽  
Growth Performance ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Rumen Fermentation ◽  
Corn Silage ◽  
Corn Straw ◽  
Rumen Microbiota ◽  
Whole Plant

Abstract BackgroundIn recent years, whole-plant corn silage had been widely used in China. Roughage was an important source of nutrition for ruminants and had an important effect on rumen microbiota, which plays an important role in animal growth performance and feed digestion. To better understand the effects of different silages on rumen microbiota, the effects of whole-plant corn silage or corn straw silage on growth performance, rumen fermentation products, and rumen microbiota of Simmental hybrid cattle were studied. Results60 healthy Simmental hybrid cattle were randomly divided into 2 groups with 6 repeats in each group and 5 cattle in each group. They were fed with whole-plant corn silage (WS) diet and corn straw silage (CS) diet respectively. Compared with corn straw silage, whole-plant corn silage significantly increased daily gain and decreased feed-weight ratio of beef cattle. Whole-plant corn silage also decreased the acetic acid in the rumen and the acetate to propionate (A/P) ratio compared with corn straw silage. At the genus level, the relative abundance of Prevotella_1 was significantly increased while the relative abundance of Succinivibrionaceae_UCG-002, Succiniclasticum, norank_f_Bacteroidales_RF16_group, and Ruminococcus_1 was decreased in cattle fed whole-plant corn silage compared with those fed corn straw silage. Prevotella_1 was positively correlated with acetic acid and A/P ratio, Succinivibrionaceae_UCG-002 was positively correlated with propionic acid and butyric acid, and negatively correlated with pH, Succiniclasticum was positively correlated with pH and A/P ratio, and norank_f__F082 and Rikenellaceae_RC9_gut_group were positively correlated with pH, propionic acid and butyric acid. Feeding whole-plant corn silage improved amino acid metabolism, nucleotide metabolism, and metabolism of beef cattle compared with feeding corn straw silage. Correlation analysis between rumen microbiota and metabolic pathways showed that Succinivibrionaceae_UCG-002 was negatively correlated with Carbohydrate Metabolism, Glycan Biosynthesis, and Metabolism, while Prevotellaceae_UCG-003 was positively correlated with Amino Acid Metabolism, and Carbohydrate Metabolism. ConclusionsFeeding whole-plant corn silage can improve the production performance rumen fermentation of beef cattle by altering rumen microbiota, amino acid metabolism, and nucleotide metabolism.

scholarly journals Effect of Methionine Supplementation on Rumen Microbiota, Fermentation, and Amino Acid Metabolism in In Vitro Cultures Containing Nitrate

2021 ◽  
Vol 9 (8) ◽  
pp. 1717
Author(s):  
Faiz-ul Hassan ◽  
Yanxia Guo ◽  
Mengwei Li ◽  
Zhenhua Tang ◽  
Lijuan Peng ◽  
...  
Keyword(s):  
Amino Acid ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Culture Technique ◽  
Rumen Microbiota ◽  
Ruminal Ph ◽  
Ch4 Production ◽  
Low Protein ◽  
The Individual

This study evaluated the effect of methionine on in vitro methane (CH4) production, rumen fermentation, amino acid (AA) metabolism, and rumen microbiota in a low protein diet. We evaluated three levels of methionine (M0, 0%; M1, 0.28%; and M2, 1.12%) of in the presence of sodium nitrate (1%) in a diet containing elephant grass (90%) and concentrate (10%). We used an in vitro batch culture technique by using rumen fluid from cannulated buffaloes. Total gas and CH4 production were measured in each fermentation bottle at 3, 6, 9, 12, 24, 48, 72 h of incubation. Results revealed that M0 decreased (p < 0.001) the total gas and CH4 production, but methionine exhibited no effect on these parameters. M0 decreased (p < 0.05) the individual and total volatile fatty acids (VFAs), while increasing (p < 0.05) the ruminal pH, acetate to propionate ratio, and microbial protein content. Methionine did not affect ruminal AA contents except asparagine, which substantially increased (p = 0.003). M2 increased the protozoa counts, but both M0 and M1 decreased (p < 0.05) the relative abundance of Firmicutes while increasing (p < 0.05) the Campilobacterota and Proteobacteria. However, Prevotella and γ-Proteobacteria were identified as biomarkers in the nitrate group. Our findings indicate that methionine can increase ruminal asparagine content and the population of Compylobactor.

scholarly journals Liver transcriptome profiling of beef steers with divergent growth rate, feed intake, or metabolic body weight phenotypes1

2019 ◽  
Vol 97 (11) ◽  
pp. 4386-4404 ◽  
Author(s):  
Robert Mukiibi ◽  
Michael Vinsky ◽  
Kate Keogh ◽  
Carolyn Fitzsimmons ◽  
Paul Stothard ◽  
...  
Keyword(s):  
Body Weight ◽  
Beef Cattle ◽  
Feed Intake ◽  
Molecular Mechanisms ◽  
Molecular Genetic ◽  
Average Daily Gain ◽  
Differentially Expressed ◽  
Beef Steers ◽  
Biological Functions ◽  
Component Traits

Abstract Average daily gain (ADG) and daily dry matter intake (DMI) are key determinants of beef industry profitability. These traits together with metabolic body weight (MWT) are combined as component traits to calculate residual feed intake (RFI), a common measure of feed efficiency in beef cattle. Recently, there have been significant efforts towards molecular genetic characterization of RFI through transcriptomic studies in different breeds and tissues. However, molecular mechanisms of RFI component traits still remain predominately unexplored. Therefore, in the current study, we investigated the hepatic transcriptomic profiles and their associations with ADG, DMI, and MWT in Angus, Charolais, and Kinsella Composite (KC) populations through global RNAseq analyses. In each population and for each trait, 12 steers with extreme phenotypes (n = 6 low and n = 6 high) were analyzed for differential gene expression. These animals were from 20 beef steers of each Angus, Charolais, and KC breed population that were initially selected for a transcriptome study of RFI. At a false discovery rate <0.05 and fold change >1.5, we identified 123, 102, and 78 differentially expressed (DE) genes between high- and low-ADG animals of Angus, Charolais, and KC populations, respectively. For DMI, 108, 180, and 156 DE genes were identified between high- and low-DMI from Angus, Charolais, and KC populations, respectively, while for MWT, 80, 82, and 84 genes were differentially expressed between high- and low-MWT animals in Angus, Charolais, and KC populations, respectively. The identified DE genes were largely breed specific (81.7% for ADG, 82.7% for DMI, and 83% for MWT), but were largely involved in the same biological functions across the breeds. Among the most enriched biological functions included metabolism of major nutrients (lipids, carbohydrates, amino acids, vitamins, and minerals), small molecule biochemistry, cellular movement, cell morphology, and cell-to-cell signaling and interaction. Notably, we identified multiple DE genes that are involved in cholesterol biosynthesis, and immune response pathways for the 3 studied traits. Thus, our findings present potential molecular genetic mechanisms and candidate genes that influence feed intake, growth, and MWT of beef cattle.

scholarly journals PSXIV-23 Characterization of microbial communities associated with the rumen lining, digesta and rumen fluid from beef cattle consuming a high energy diet using 16S rRNA gene amplicon sequencing

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 446-446
Author(s):  
Arquimides Reyes ◽  
Margaret Weinroth ◽  
Cory Wolfe ◽  
Robert Delmore ◽  
Terry Engle ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Beef Cattle ◽  
Microbial Communities ◽  
Relative Abundance ◽  
Rumen Fluid ◽  
Amplicon Sequencing ◽  
High Energy ◽  
Feedlot Cattle ◽  
Rrna Gene

Abstract The true etiology of liver abscesses is not well known. Therefore, the objective of this study was to characterize the microbial communities in the rumen lining, digesta, and rumen fluid from beef cattle consuming a high energy diet, using 16S rRNA gene amplicon sequencing. Twelve crossbred feedlot steers (450 ±10 kg; ~ 3.0 years of age) fitted with ruminal fistulas, consuming a high energy finishing diet (1.43 NEg, Mcal/kg DM) for 21 d were utilized in this experiment. Microbial DNA from three regions within the rumen [rumen lining (ventral/lateral), digesta (geometric center of the rumen), and rumen fluid] was extracted and the V4 region of the 16S rRNA gene was amplified and sequenced. Across all sample regions, bacterial sequences were classified into 34 phyla, 76 classes, 143 orders, and 254 families. Bacteroidetes and Firmicutes were the predominant phyla present across all samples. The relative abundance of Bacteroidetes detected in rumen fluid was lesser (P &lt; 0.05) when compared to bacteria sampled from the rumen lining and digesta. In contrast, the relative abundance of Firmicutes were greater (P &lt; 0.05) in rumen fluid and the rumen lining when compared to digesta samples. There are very few publications describing the complex community of the rumen microbiome. To our knowledge this is the first publication categorizing microbial populations in three distinct locations within the rumen using next generation sequencing in feedlot cattle.

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