scholarly journals Sequence Variation in the Bovine Lipin-1 Gene (LPIN1) and Its Association with Milk Fat and Protein Contents in New Zealand Holstein-Friesian × Jersey (HF × J)-cross Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3223
Author(s):  
Xiaohua Du ◽  
Huitong Zhou ◽  
Xia Liu ◽  
Yunhai Li ◽  
Jonathan G. H. Hickford
Keyword(s):  
New Zealand ◽  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Sequence Variation ◽  
Polymorphism Analysis ◽  
Sequence Variants ◽  
Coding Region ◽  
Holstein Friesian ◽  
Lipin 1

Lipin-1 is known to play a regulatory role in tissues that function in lipid metabolism. In dairy cows, the lipin-1 gene (LPIN1) is highly expressed in the mammary gland, but its function in milk production is less understood. In this study, we used PCR-single strand conformation polymorphism analysis to investigate sequence variation in three regions of bovine LPIN1 in New Zealand Holstein-Friesian × Jersey (HF × J)-cross dairy cows, including part of the 5′ non-coding region, the region containing the LPIN1β-spliced exon, and the sixth coding exon that encodes the putative transcriptional activating domain of the protein. No variation was found in the LPIN1β-spliced exon, but two sequence variants containing one single nucleotide polymorphism (SNP) were identified in the 5′ non-coding region and four sequence variants containing four non-synonymous SNPs were identified in the sixth coding exon. Among the three common variants of the sixth coding exon, variant C was found to be associated with an increase in milk fat percentage (presence 4.96 ± 0.034% vs. absence 4.81 ± 0.050%; p = 0.006) and milk protein percentage (presence 4.09 ± 0.017% vs. absence 3.99 ± 0.025%; p = 0.001), but no associations (p > 0.01) were detected for milk yield. These results suggest that variation in LPIN1 affect the synthesis of fat and proteins in milk and has potential as a gene-marker to improve milk production traits.

scholarly journals Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1447
Author(s):  
Ishaku L. Haruna ◽  
Yunhai Li ◽  
Ugonna J. Ekegbu ◽  
Hamed Amirpour-Najafabadi ◽  
Huitong Zhou ◽  
...  
Keyword(s):  
Fatty Acid ◽  
New Zealand ◽  
Nucleotide Sequence ◽  
Milk Fat ◽  
Linear Mixed Effect Model ◽  
Polymorphism Analysis ◽  
Mixed Effect ◽  
Myostatin Gene ◽  
Holstein Friesian ◽  
Nucleotide Sequence Variation

The myostatin gene (MSTN), which encodes the protein myostatin, is pleiotropic, and its expression has been associated with both increased and decreased adipogenesis and increased skeletal muscle mass in animals. In this study, the polymerase chain reaction, coupled with single strand conformation polymorphism analysis, was utilized to reveal nucleotide sequence variation in bovine MSTN in 410 New Zealand (NZ) Holstein-Friesian × Jersey (HF × J)-cross cows. These cows ranged from 3 to 9 years of age and over the time studied, produced an average 22.53 ± 2.18 L of milk per day, with an average milk fat content of 4.94 ± 0.17% and average milk protein content of 4.03 ± 0.10%. Analysis of a 406-bp amplicon from the intron 1 region, revealed five nucleotide sequence variants (A–E) that contained seven nucleotide substitutions. Using general linear mixed-effect model analyses the AD genotype was associated with reduced C10:0, C12:0, and C12:1 levels when compared to levels in cows with the AA genotype. These associations in NZ HF × J cross cows are novel, and they suggest that this variation in bovine MSTN could be explored for increasing the amount of milk unsaturated fatty acid and decreasing the amount of saturated fatty acid.

Replacing wheat with canola meal and maize grain in the diet of lactating dairy cows: Feed intake, milk production and cow condition responses

2017 ◽  
Vol 84 (3) ◽  
pp. 240-247 ◽  
Author(s):  
Ruairi P McDonnell ◽  
Martin vH Staines
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Protein ◽  
Daily Intake ◽  
Canola Meal ◽  
Grass Silage ◽  
Holstein Friesian ◽  
Maize Grain ◽  
The Difference

This research paper describes the effect of partially replacing wheat with maize grain and canola meal on milk production and body condition changes in early lactation Holstein-Friesian dairy cows consuming a grass silage-based diet over an 83-d period. Two groups of 39 cows were stratified for age, parity, historical milk yield and days in milk (DIM), and offered one of two treatment diets. The first treatment (CON) reflected a typical diet used by Western Australian dairy producers in summer and comprised (kg DM/cow per d); 8 kg of annual ryegrass silage, 6 kg of crushed wheat (provided once daily in a mixed ration), 3·6 kg of crushed lupins (provided in the milking parlour in two daily portions) and ad libitum lucerne haylage. The second treatment diet (COMP) was identical except the 6 kg of crushed wheat was replaced by 6 kg of a more complex concentrate mix (27% crushed wheat, 34% maize grain and 37% canola meal). Lucerne haylage was provided independently in the paddock to all cows, and no pasture was available throughout the experiment. The COMP group had a greater mean overall daily intake (22·5vs20·4 kg DM/cow) and a higher energy corrected milk (ECM) yield (29·2vs27·1 kg/cow;P= 0·047) than the CON cows. The difference in overall intake was caused by a higher daily intake of lucerne haylage in COMP cows (4·5vs2·3 kg DM/cow). The CON group had a higher concentration of milk fat (42·1vs39·3 g/kg;P= 0·029) than COMP cows. Milk protein yield was greater in COMP cows (P< 0·021); however, milk fat yield was unaffected by treatment. It is concluded that partially replacing wheat with canola meal and maize grain in a grass silage-based diet increases voluntary DMI of conserved forage and consequently yields of ECM and milk protein.

Effect of timing of protein supplementation on the performance of lactating dairy cows

2005 ◽  
Vol 45 (4) ◽  
pp. 337
Author(s):  
B. C. Granzin
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Protein Supplementation ◽  
Net Energy ◽  
Maize Silage ◽  
Holstein Friesian ◽  
Grazing Dairy Cows ◽  
And Control ◽  
Friesian Cows

Two experiments were undertaken to determine the effect of timing of protein supplementation on performance of grazing, lactating Holstein–Friesian cows fed maize silage and grain-based concentrate. In experiment 1, 36 cows were fed 0.8 kg DM/day of solvent-extracted cottonseed meal (CSM) either as 1 meal at 1200 hours with maize silage (CSM 1200) or at 1530 hours with concentrate (CSM 1530), or in 2 meals at 0600 and 1530 hours with concentrate (CSM 600 + 1530). In experiment 2, 36 cows were either fed no CSM (control) or fed 1.0 kg DM/day as either CSM 1200 or CSM 600 + 1530. In experiment 1, daily yields of 4% fat-corrected milk (FCM) and milk fat for CSM 600 + 1530 were significantly higher than for CSM 1530 with respective means of 22.8 v. 20.7 L and 895 v. 804 g. Daily yields of FCM and milk fat for CSM 1200 were intermediate (21.7 L and 841 g/cow, respectively). A similar trend for daily protein yield per cow was noted (712, 695 and 666 g for CSM 600 + 1530, 1200 and 1530, respectively). In experiment 2, milk yield differed numerically between CSM 600 + 1530 and other treatments, with means (± s.e.d.) of 24.7 ± 0.78, 22.9 ± 0.78 and 22.9 ± 0.78 L/cow.day for CSM 600 + 1530, CSM 1200 and control, respectively. Mean (± s.e.d.) net energy requirements for milk production and liveweight change tended to be lower for the control (68 ± 3.6 MJ/cow.day) as opposed to CSM 600 + 1530 (79 ± 3.6 MJ/cow.day) and CSM 1200 (76 ± 3.6 MJ/cow.day). Cumulative time where rumen degradable nitrogen:rumen degradable dry matter was less than 22 g/kg were 2, 2 and 3 h for CSM 600 + 1530, CSM 1200 and CSM 1530, respectively, in experiment 1, and 6, 4 and 2 h for the control, 1200 and CSM 600 + 1530, respectively, in experiment 2. No differences in rumen ammonia-N concentrations were noted between treatments in experiment 1. In experiment 2, a significantly lower mean (± s.e.d.) rumen ammonia-N concentration was recorded for the control at 1530 hours (62 ± 14.1 mg/dL) in comparison to CSM 600 + 1530 (114 ± 14.1 mg/dL) and CSM 1200 (119 ± 14.1 mg/dL). These experiments show that for grazing dairy cows supplemented with maize silage and grain-based concentrate, feeding a daily aliquot of CSM as 2 meals at 0600 and 1530 hours rather than 1 meal at 1200 or 1530 hours improves milk production.

scholarly journals Short-Term Effect of Daily Herbage Allowance Restriction on Pasture Condition and the Performance of Grazing Dairy Cows during Autumn

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 62 ◽  
Author(s):  
Verónica M. Merino ◽  
Oscar A. Balocchi ◽  
M. Jordana Rivero ◽  
Rubén G. Pulido
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Protein Concentration ◽  
Short Term ◽  
Leaf Production ◽  
Sward Height ◽  
Milk Output ◽  
Holstein Friesian ◽  
Grazing Dairy Cows

The aim of this study was to evaluate the short-term effects of daily herbage allowance (DHA, defined as the product of pre-grazing herbage mass and offered area per animal) on pasture conditions and milk production of Holstein-Friesian dairy cows. Forty-four early lactation dairy cows were randomly assigned to one of four treatments in a 2 × 2 factorial design that tested two levels of DHA (17 and 25 kg DM/cow.day) and two levels of maize silage supplementation (MSS, 4.5 and 9 kg DM/cow.day) over a 77-day period. Low DHA decreased the post-grazing herbage mass from 1546 to 1430 kg DM/ha and the compressed sward height from 5 to 4.4 cm, while the grazing efficiency remained unaffected. Low DHA induced a faster herbage mass reduction, while the sward-height and pasture characteristics did not differ from the high DHA regime. Low DHA decreased the tiller production rates and daily lamina growth, while the leaf-production rate was not affected by the DHA. Daily increases of herbage mass were greater in the high DHA than in the low DHA treatments. Individual milk production and milk protein concentration decreased at a low DHA compared to high DHA, while the milk fat concentration was greater and the milk output per hectare increased by 1510 kg. Neither the MSS level nor the interaction DHA by the MSS level had any effect on the sward characteristics or the productivity of the cows. From these results, it is suggested that, in a high-quality pasture, using 17 kg DM/cow.day was appropriate for improving both herbage utilization and milk production per hectare while maintaining the short-term conditions of a pasture grazed by dairy cows in the autumn.

scholarly journals Metabolic and Productive Response and Grazing Behavior of Lactating Dairy Cows Supplemented with High Moisture Maize or Cracked Wheat Grazing at Two Herbage Allowances in Spring

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 919
Author(s):  
Verónica M. Merino ◽  
Lorena Leichtle ◽  
Oscar A. Balocchi ◽  
Francisco Lanuza ◽  
Julián Parga ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Dry Matter ◽  
Body Condition Score ◽  
Latin Square ◽  
Ruminal Fermentation ◽  
High Moisture ◽  
Plasma Urea ◽  
Grazing Behavior

The aim was to determine the effect of the herbage allowance (HA) and supplement type (ST) on dry matter intake (DMI), milk production and composition, grazing behavior, rumen function, and blood metabolites of grazing dairy cows in the spring season. Experiment I: 64 Holstein Friesian dairy cows were distributed in a factorial design that tested two levels of daily HA (20 and 30 kg of dry matter (DM) per cow) and two ST (high moisture maize (HMM) and cracked wheat (CW)) distributed in two daily rations (3.5 kg DM/cow/day). Experiment II: four mid-lactation rumen cannulated cows, supplemented with either HMM or CW and managed with the two HAs, were distributed in a Latin square design of 4 × 4, for four 14-d periods to assess ruminal fermentation parameters. HA had no effect on milk production (averaging 23.6 kg/day) or milk fat and protein production (823 g/day and 800 g/day, respectively). Cows supplemented with CW had greater protein concentration (+1.2 g/kg). Herbage DMI averaged 14.17 kg DM/cow.day and total DMI averaged 17.67 kg DM/cow.day and did not differ between treatments. Grazing behavior activities (grazing, rumination, and idling times) and body condition score (BCS) were not affected by HA or ST. Milk and plasma urea concentration increased under the high HA (+0.68 mmol/L and +0.90 mmol/L, respectively). Cows supplemented with HMM had lower milk and plasma urea concentrations (0.72 mmol/L and 0.76 mmol/L less, respectively) and tended (p = 0.054) to have higher plasma β-hydroxybutyrate. Ruminal parameters did not differ between treatments.

scholarly journals Identification of Genomic Regions Associated with Concentrations of Milk Fat, Protein, Urea and Efficiency of Crude Protein Utilization in Grazing Dairy Cows

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 456
Author(s):  
Hewa Bahithige Pavithra Chathurangi Ariyarathne ◽  
Martin Correa-Luna ◽  
Hugh Thomas Blair ◽  
Dorian John Garrick ◽  
Nicolas Lopez-Villalobos
Keyword(s):  
Gene Ontology ◽  
New Zealand ◽  
Dairy Cows ◽  
Candidate Genes ◽  
Milk Fat ◽  
Crude Protein ◽  
Single Locus ◽  
Protein Utilization ◽  
Grazing Dairy Cows ◽  
Genomic Regions

The objective of this study was to identify genomic regions associated with milk fat percentage (FP), crude protein percentage (CPP), urea concentration (MU) and efficiency of crude protein utilization (ECPU: ratio between crude protein yield in milk and dietary crude protein intake) using grazing, mixed-breed, dairy cows in New Zealand. Phenotypes from 634 Holstein Friesian, Jersey or crossbred cows were obtained from two herds at Massey University. A subset of 490 of these cows was genotyped using Bovine Illumina 50K SNP-chips. Two genome-wise association approaches were used, a single-locus model fitted to data from 490 cows and a single-step Bayes C model fitted to data from all 634 cows. The single-locus analysis was performed with the Efficient Mixed-Model Association eXpedited model as implemented in the SVS package. Single nucleotide polymorphisms (SNPs) with genome-wide association p-values ≤ 1.11 × 10−6 were considered as putative quantitative trait loci (QTL). The Bayes C analysis was performed with the JWAS package and 1-Mb genomic windows containing SNPs that explained > 0.37% of the genetic variance were considered as putative QTL. Candidate genes within 100 kb from the identified SNPs in single-locus GWAS or the 1-Mb windows were identified using gene ontology, as implemented in the Ensembl Genome Browser. The genes detected in association with FP (MGST1, DGAT1, CEBPD, SLC52A2, GPAT4, and ACOX3) and CPP (DGAT1, CSN1S1, GOSR2, HERC6, and IGF1R) were identified as candidates. Gene ontology revealed six novel candidate genes (GMDS, E2F7, SIAH1, SLC24A4, LGMN, and ASS1) significantly associated with MU whose functions were in protein catabolism, urea cycle, ion transportation and N excretion. One novel candidate gene was identified in association with ECPU (MAP3K1) that is involved in post-transcriptional modification of proteins. The findings should be validated using a larger population of New Zealand grazing dairy cows.

Sequence variants in the FcεRI alpha chain gene

2002 ◽  
Vol 93 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Toshiki Shikanai ◽  
Eric S. Silverman ◽  
Brian W. Morse ◽  
Craig M. Lilly ◽  
Hiroshi Inoue ◽  
...  
Keyword(s):  
Promoter Region ◽  
Population Substructure ◽  
Chain Gene ◽  
Polymorphism Analysis ◽  
Sequence Variants ◽  
Nucleotide Polymorphisms ◽  
Coding Region ◽  
Alpha Chain ◽  
Asthmatic Patients ◽  
Single Stranded Conformational Polymorphism

There is a relationship between IgE levels and expression of high-affinity IgE receptors (FcεRI). Because the alpha chain is the only portion of the receptor that binds directly to IgE, we reasoned that sequence variants in the FcεRI alpha gene may exist that alter these binding events. We screened all of the exons and the promoter region of the FcεRI alpha chain gene with genomic DNA from 389 asthmatic and 341 normal control subjects for mutations by using single-stranded conformational polymorphism analysis. No nonsynonomous single nucleotide polymorphisms (SNPs) were identified in the coding region. Three SNPs were found in the promoter region: an A/C transversion at −770 from the translation start site; a G/A transition at −664; and a T/C transition at −335. No differences in allele frequencies were detected between asthmatic subjects and controls. Homozygosity for the C variant at locus −335 was more common in Caucasian asthmatic patients with IgE levels in the lower quartile than in the upper quartile ( P = 0.032). An analysis of highly polymorphic SNPs indicated that this association is unlikely to be due to population substructure. We conclude that homozygosity for the C allele of FcεRI alpha chain variant is associated with lower IgE levels.

Effects of feeding peas to high-producing dairy cows

1995 ◽  
Vol 75 (4) ◽  
pp. 625-629 ◽  
Author(s):  
R. R. Corbett ◽  
L. A. Goonewardene ◽  
E. K. Okine
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Dairy Herd ◽  
Protein Source ◽  
Canola Meal ◽  
Stage Of Lactation ◽  
Nutrient Analyses ◽  
Protein Milk

The effect of substituting peas for soybean and canola meals as a protein source in a high-producing dairy herd was studied in 66 Holstein cows, divided into two groups based on stage of lactation, parity, level of milk production and days in milk. Two 18.5% crude protein grain concentrate diets were formulated based on the nutrient analyses of the forages available. The control grain mix contained standard protein sources, principally soybean and canola meal (SBM\CM) while the test grain mix was formulated to contain approximately 25% field peas as the major source of protein. Both grain rations were formulated to the same nutrient specifications and balanced for undegradable protein. The duration of the trial was 6 mo during which grain feeding levels were adjusted monthly based on milk yield. For cows in early lactation, 4% fat-corrected milk yield was higher (P < 0.05) for cows fed pea based concentrates (31.3 kg d−1) than for cows fed SBM\CM supplement (29.7 kg d−1). Fat-corrected milk yield was not affected by source of protein in mid- and late-lactation cows. Fat-corrected milk production was not different (P > 0.05) for cows fed SBM\CM compared with cows fed the pea supplement when cows across all stages of lactation were included in the analyses. Milk fat percent was significantly higher (P < 0.05) for early- and mid-lactation cows fed the pea supplement. The results suggest that peas can be substituted for SBM\CM as a protein source for high-producing dairy cows. Key words: Dairy cow, pea, soybean and canola meal supplement, undegradable protein, milk production

The Effect of Concentrate Type and Supplementary Lactic Acid or Soya-Oil on Milk Production in Dairy Cows Offered Restricted or Extensively Fermented Grass Silages

1994 ◽  
Vol 1994 ◽  
pp. 38-38
Author(s):  
J G Doherty ◽  
C S Mayne
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Composition ◽  
Fresh Weight ◽  
Single Strain ◽  
High Fibre ◽  
Soyabean Meal ◽  
High Starch ◽  
Aliphatic Carboxylic Acids

Several studies have shown increased silage dry-matter intake (SDMI) and improved milk fat concentrations in dairy cows offered restricted fermented grass silages compared to more extensively fermented silages. A recent study suggested that differences in silage intakeper serather than an alteration in rumen fermentation may be responsible for the changes in milk composition observed in the previous studies (Doherty and Mayne, 1993). The aim of the present study was to examine the effect of changes in concentrate composition on milk production parameters in dairy cows offered grass silages of contrasting fermentation type.Two direct cut grass silages were prepared using either an inoculant, containing a single strain ofLactobacillus plantarum, (Ecosyl, 3 1/t fresh weight, Zeneca Products Ltd) or a mixture of aliphatic carboxylic acids (Maxgrass, 6 1/t fresh weight, BP Chemicals Ltd). Two concentrates (high starch or high fibre) were formulated containing either: barley, 300; wheat, 355; and soyabean-meal, 270 g/kg (high starch) or unmolassed sugar-beet pulp, 555; citrus pulp, 100; and soyabean-meal, 270 g/kg (high fibre).

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