scholarly journals Addition of Different Levels of Humic Substances Extracted from Worm Compost in Broiler Feeds

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3199
Author(s):  
Alejandra Domínguez-Negrete ◽  
Sergio Gómez-Rosales ◽  
María de Lourdes Angeles ◽  
Luis Humberto López-Hernández ◽  
Tercia Cesaria Reis de Souza ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Humic Substances ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Growth Promoter ◽  
Growth Promoters

Different sources and inclusion levels of humic substances (HS) have been tested in broiler rations as an alternative to the addition of growth promoter antibiotics (GPA) with promising results. The current study was carried out to assess the influence of HS extracted from worm compost on broiler production parameters, carcass yield, tibia characteristics, lactic acid bacteria (LAB) counts, excretion of Eimeria oocysts, and antioxidant status of breast meat. A total of 1200 broilers were used, housed in groups of 30 per pen, and assigned to five treatments: 1 = basal diet with GPA (positive control), 2 = basal diet without GPA (negative control), 3–5 = basal diet with 0.15, 0.30, and 0.45% HS, respectively. The data was subjected to a variance analysis and orthogonal contrasts. The FI decreased linearly (p < 0.05) from 1–14, 29–42, and 1–42 days as the inclusion of HS in the feed increased. The FCR had quadratic responses (p < 0.01) from 29–42 and 1–42 days concerning the HS inclusion levels. Lactic acid bacteria was higher (p ˂ 0.05) in ten-day-old chicks with 0.45% HS in the diet. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity antioxidant potential decreased linearly (p < 0.05) concerning increasing HS in the feed. The results indicate that HS can be used as growth promoters in broiler feeds.

scholarly journals The use of lactic acid bacteria isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), as growth promoters in fish fed low protein diets

2017 ◽  
Vol 41 (3) ◽  
pp. 490-497
Author(s):  
Maurilio Lara-Flores ◽  
Miguel A. Olvera-Novoa
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Intestinal Tract ◽  
Control Diet ◽  
Negative Control ◽  
Growth Promoter ◽  
Growth Promoters ◽  
Nile Tilapia Oreochromis Niloticus

In this study, the effect as growth promoter of five lactic acid strains (Enterococcus faecium, E. durans, Leuconostoc sp., Streptococcus sp. I and Streptococcus sp. II), isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), was evaluated. Eight isocaloric diets were formulated: one containing 40% of protein as positive control, and seven with 27% protein. Five diets with 27% protein were supplemented with one of the isolated lactic acid bacteria in a concentration of 2.5x106 cfu g-1 of diet. A commercial probiotic based on S. faecium and Lactobacillus acidophilus was added at the same concentration to one 27% protein diet as a comparative diet, and the last diet was not supplemented with bacteria (negative control). Tilapia fry (280 mg basal weight) stocked in 15 L aquaria at a density of two per liter were fed for 12 weeks with experimental diets. Results showed that fry fed with native bacteria supplemented diets presented significantly higher growth and feeding performance than those fed with control diet. Treatment with Streptococcus sp. I isolated from the intestine of Tilapia produced the best growth and feeding efficiency, suggesting that this bacteria is an appropriate native growth promoter.

scholarly journals Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits

2020 ◽  
Vol 65 (No. 1) ◽  
pp. 23-30 ◽  
Author(s):  
Heping Zhao ◽  
Feike Zhang ◽  
Jun Chai ◽  
Jianping Wang
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Positive Control ◽  
Negative Control ◽  
Listeriolysin O ◽  
Serum Iga ◽  
Probiotic Lactic Acid Bacteria ◽  
Spleen And Lymph Nodes ◽  
Proinflammatory Factors

The present study aimed to investigate the effect of probiotic lactic acid bacteria (LAB) addition on Listeria monocytogenes translocation and its toxin listeriolysin O (LLO), proinflammatory factors, immune organ indexes and serum immunoglobulins in farmed rabbits. Five treatments included negative control (NC), positive control (PC) with L. monocytogenes infection and supplemental LAB at 3.0 × 10<sup>6 </sup>(low-LAB, L-LAB), 3.0 × 10<sup>8</sup> (medium-LAB, M-LAB) and 3.0 × 10<sup>10 </sup>(high-LAB, H-LAB) CFU/kg of diet, respectively. The LAB was a mixture of equal amounts of Lactobacillus acidophilus (ACCC11073), Lactobacillus plantarum (CICC21863) and Enterococcus faecium (CICC20430). A total of 180 weaned rabbits (negative for L. monocytogenes) were randomly assigned to 5 groups with 6 replicates of 6 rabbits each in response to the 5 treatments. L. monocytogenes infection occurred on the first day of feeding trial and dietary LAB supplementation lasted for 14 days. The results showed that on days 7 and 14 post administration, L. monocytogenes in caecum, liver, spleen and lymph nodes was reduced in M-LAB and H-LAB compared to PC (P &lt; 0.05), and linear and quadratic reducing trends were found in liver on day 7 (P ≤ 0.002). On day 14, mucosa LLO mRNA expression and serum TNFα, IL1β and IFNγ were reduced in the three LAB treatments (P &lt; 0.05), and linear and quadratic trends were found on TNFα and IL1β (P ≤ 0.025); indexes of thymus and spleen, serum IgA and IgG were increased in the LAB treatments (P &lt; 0.05). It is concluded that LAB can be used to alleviate L. monocytogenes infection and to improve the immune function of farmed animals.

scholarly journals Growth performance, nutrient digestibility, and selected fecal microbiota are improved by β-glucan supplementation in weaner pigs

2018 ◽  
Vol 18 (3) ◽  
pp. 769-779
Author(s):  
Xin Jian Lei ◽  
Hyeok Min Yun ◽  
Yi Yang ◽  
In Ho Kim
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Growth Performance ◽  
Dry Matter ◽  
Basal Diet ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Coliform Bacteria ◽  
Weaner Pigs ◽  
Blood Profiles

Abstract This study was conducted to evaluate the effects of dietary β-glucan supplementation on growth performance, nutrient digestibility, blood profiles, and fecal characteristics in weaner pigs. A total of 140 weaner pigs [(Yorkshire × Landrace) × Duroc] with an average body weight of 6.37±1.14 kg were allotted to one of the following dietary treatments: 1) negative control (NC, basal diet); 2) positive control (PC, basal diet supplemented with 39 ppm Tiamulin); 3) basal diet supplemented with 0.1% β-glucan (G1); 4) basal diet supplemented with 0.2% β-glucan (G2). During days 22 to 42 and 1 to 42, pigs offered PC and G2 diets grew faster than those offered NC diet (P<0.05). Additionally, during the same periods, pigs fed G2 and PC diets exhibited improved gain:feed ratio compared to pigs receiving NC and G1 diets (P<0.05). Pigs fed G2 diet exhibited higher coefficient of total tract apparent digestibility of dry matter than those fed NC diet (P<0.05). There were no differences on blood profiles, fecal scores, fecal moisture, and fecal pH (P>0.05). Fecal lactic acid bacteria counts in G2 treatment were higher compared with PC and NC treatments (P<0.05). Coliform bacteria concentrations were decreased in pigs fed PC and G2 diets compared with those fed NC diet (P<0.05). In conclusion, the results of the current study indicate that dietary supplementation of 0.2% β-glucan can improve growth performance and dry matter digestibility, increase fecal lactic acid bacteria concentration but decrease fecal coliform bacteria concentration in weaner pigs.

scholarly journals Dietary supplementation of Neem (Azadirachta indica) leaf extracts improved growth performance and reduced production cost in broilers

2020 ◽  
Vol 13 (6) ◽  
pp. 1050-1055
Author(s):  
Torun Kumar Paul ◽  
Md. Mehedi Hasan ◽  
Md. Anowarul Haque ◽  
Sudipta Talukder ◽  
Yousuf Ali Sarker ◽  
...  
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Leaf Extracts ◽  
Neem Leaves

Background and Aim: Global trend to remove the antibiotic growth promoter (AGP) from animals contributes to the exploration of successful measures to sustain production and reduces the intestinal diseases in the post-AGP era. Plant extracts, therefore, have been used to improve performance and intestinal health. Here, we conducted a study to evaluate the effects of neem (Azadirachta indica) leaf extracts (NLE) as alternatives to AGPs in broiler chickens. Materials and Methods: Sixty day-old broiler chicks were assigned to 12-floor pens, each stocked with five birds and divided into three treatment groups of four pens per treatment. The groups were: Negative control, basal diet without additives; positive control, basal diet with antibiotics and vitamins; and NLE treated group, basal diet supplemented with 0.1% aqua extract of neem leaves. Results: Overall feed intake was significantly (p≤0.05) highest in the positive control. Higher body weight, higher dressing percentage, and lower feed conversion ratio were observed in birds treated with NLE compared to the negative control group (p≤0.05) but not the positive control group (p>0.05). There was no significant variation in hematology between different groups. Furthermore, the economic evaluation indicated that the NLE treatment was found cheaper than control and antibiotic treatment in cost-benefit analysis. Conclusion: We suggest NLE might be a cheaper alternative to antibiotics in broiler production as indicated by improved body weight and feed efficiency.

scholarly journals Phytogenic additive as an alternative to growth promoters in broiler chickens

2009 ◽  
Vol 39 (2) ◽  
pp. 522-527 ◽  
Author(s):  
Gerson Neudí Scheuermann ◽  
Anildo Cunha Junior ◽  
Lucas Cypriano ◽  
Alexandre Mossate Gabbi
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Positive Control ◽  
Negative Control ◽  
Feed Additive ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Growth Promoters ◽  
The Cost

This study evaluated a phytogenic feed additive for broiler chickens. A total of 1,632 broiler chicks were distributed into four treatments: negative control (without growth promoter); positive control (avilamycine, 10ppm + colistin, 15ppm); and two alternative treatments with 150ppm of phytogenic additive, one with a reduced Ca and P levels diet (PA-R1) and the other with lower energy, and amino acids, besides Ca and P (PA-R2). The trial was conducted with 12 replicates, each consisted of a pen with 34 birds. The alternative diets showed body weight intermediate to the two controls at 42 days, with no significant (P>0.05) treatment effect on feed conversion ratio. No treatment differences (P>0.05) on carcass yield and composition was observed. There was a tendency of abdominal fat lipids saturation, when the phytogenic additive was used, as possible consequence of a decreased level of soybean oil in the diets. A difference (P<0.001) on ingredient consumption profile was observed between the treatments. All together, this study showed a possibility to reduce the cost of total feed used to produce a broilers or a ton of body weight by the utilization of the tested phytogenic additive.

scholarly journals Effect of the Addition of Humic Substances as Growth Promoter in Broiler Chickens Under Two Feeding Regimens

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1101 ◽  
Author(s):  
Alejandra Domínguez-Negrete ◽  
Sergio Gómez-Rosales ◽  
María de Lourdes Angeles ◽  
Luis Humberto López-Hernández ◽  
Tercia Cesaria Reis-de Souza ◽  
...  
Keyword(s):  
Growth Performance ◽  
Humic Substances ◽  
Meat Quality ◽  
Broiler Chickens ◽  
Nutrient Balance ◽  
Positive Control ◽  
Negative Control ◽  
Growth Promoter ◽  
Oocyst Excretion ◽  
Carcass Yield

Humic substances (HS) from different sources have been evaluated to replace or reduce the use of growth promoter antibiotics (GPA) in the feeds of broiler chickens. The objective was to evaluate the growth performance, tibia measurements, nutrient balance, meat quality, and microbiological status of broiler fed with an HS extract (EHS) under ad libitum (ADLIB) or feed restriction (REST). Individually caged broilers (n = 180, 14–35 day of age) were assigned to a factorial arrangement of three dietary treatments: (1) positive control with bacitracin methylene disalicylate (BMD) and salinomycin; (2) negative control without BMD nor salinomycin, and (3) same as negative control with 0.25% EHS, and two feeding regimens 1) ADLIB or REST for 24 h on d 1, 7, and 14. Results were subjected to ANOVA. Positive control and EHS-fed broilers showed higher carcass yield (p < 0.05) and lower oocyst excretion (p < 0.01) compared to negative control birds. Lactic acid bacteria (LAB) and Clostridium perfringens (C. perfringens) were higher in negative control and EHS-broilers compared to positive control (p < 0.01). In conclusion, higher carcass yield, lower C. perfringens and oocyst excretion were found in positive control and higher carcass yield, higher LAB and lower oocyst excretion were found in EHS-fed broilers. Broilers subjected to REST had reduced growth performance and meat quality. In conclusion, EHS could be used to increase the carcass yield and beneficial LAB in broilers.

scholarly journals Antioxidant activity and short-chain fatty acid production of lactic acid bacteria isolated from Korean individuals and fermented foods

3 Biotech ◽  
2021 ◽  
Vol 11 (5) ◽  
Author(s):  
Chang-Ho Kang ◽  
Jin-Seong Kim ◽  
Hye Min Park ◽  
Seonyoung Kim ◽  
Nam-Soo Paek
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Free Radical ◽  
Lactic Acid Bacteria ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Scavenging Activity

AbstractCompounds of the cell walls of heat-killed lactic acid bacteria show immunomodulatory properties which boost immunological systems, and are used ad postbiotics (paraprobiotics). In this study, we used 17 different heat-killed isolates as postbiotics and evaluated their anti-inflammatory potential on the expression of proinflammatory mediators and cellular signaling pathways of murine macrophage, RAW 264.7 cells. Bifidobacterium bifidum MG731 showed the high 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (90.6%), followed by Bifidobacterium lactis MG741 (59.6%). The Bi. lactis MG741 showed the high ABTS free radical scavenging activity (99.5%), followed by Lactobacillus plantarum MG989 (98.9%), Lactobacillus salivarius MG242 (97.1%), and Bi. bifidum MG731 (96.1%). In addition, Bi. bifidum MG731 showed the lowest nitric oxide production (4.28 µM), followed by B. lactis MG741 (10.80 µM), L. salivarius MG242 (14.60 µM), and L. plantarum MG989 (19.60 µM). The selected strains showed a decreased nitric oxide production via downregulation of inducible nitric oxide synthase and cyclooxygenase 2, which were upregulated via LPS-stimulated RAW 264.7 macrophages. Short-chain fatty acids (SCFA) including acetic, propionic, and butyric acid were produced by four strains. The Bi. bifidum MG731 showed total SCFAs production (4998.6 µg/g), Bi. lactis MG741 (2613.9 µg/g), L. salivarius MG242 (1456.1 µg/g), and L. plantarum MG989 (630.2 µg/g). These results indicated that the various selected strains may possess an anti-inflammatory potential and provide a molecular basis for the development of functional probiotics.

scholarly journals EFEITO DA ADIÇÃO DE GENGIBRE (Zingiber officinale) EM PÓ SOBRE O DESEMPENHO DE FRANGOS DE CORTE NA FASE INICIAL

2017 ◽  
Vol 13 (Especial 2) ◽  
pp. 156-163
Author(s):  
Vilson Matias Pinto ◽  
Paulo Ricardo de Sá da Costa Leite ◽  
Mônica Maria de Almeida Brainer ◽  
Brena Cristine Rosário Silva ◽  
Leidyanne Guimarães Silva
Keyword(s):  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Production Performance ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Performance Variables ◽  
Randomized Design

The objective of this study was to evaluate the production performance of broilers with the addition of ginger (Zingiber officinale) in broiler diets in starter phases. Were used 630 broiler chicks in a completely randomized design with five treatments and seven repetitions with eighteen birds each. The treatments consist of a reference diet with corn and soybean meal and adding different levels of powdered ginger, five treatments, consisting of positive control, negative control and three levels of ginger (0.50%, 0.75%; 1.0%). Were evaluated at the end of each phase, feed intake, weight gain, feed conversion, body weight, pancreatic weight and length and weight of the intestine. The data will be submitted to analysis of variance and means to regression analysis with 5% significance. No effects of the treatments on the performance variables were observed in the initial stage of creation. With this result, at twenty-one days old, we observed the potential for substitution of growth promoters for powdered ginger. The inclusion of up to 1% of powdered ginger did not affect bird performance providing results similar to positive control treatments with growth promoter.

scholarly journals Efficacy of organic acids as an alternative to antibiotic growth promoters in weaned pigs

2018 ◽  
Vol 17 (06) ◽  
pp. 8-14
Author(s):  
Tung M. Che
Keyword(s):  
Organic Acids ◽  
Antibiotic Treatment ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Growth Promoters ◽  
Weaned Pigs ◽  
E Coli ◽  
Diarrhea Incidence

The objective of the experiment was to compare effects of dietary supplementation of organic acids (ProHacid Advance, PRO) and antibiotics on growth performance, diarrhea incidence, frequency of antibiotic treatment, and E. coli shedding in weaned pigs. A total of 224 crossbred weaned pigs [(Yorkshire x Landrace) x Duroc; 29 days old)] were allotted to 1 of 4 treatments with 7 replicate pens of 8 pigs each according to sex, litter origin and weight in an RCBD. The 4 dietary treatments included (1) basal diet + 50 mg/kg neomycin and 10 mg/kg enramycin (positive control, PC), (2) basal diet without antibiotics (negative control, NC), (3) As 2 + 0.25% PRO, and (4) As 2 + 0.5% PRO. Over a 4-week study, the results showed that there were no differences in the average daily gain and average daily feed intake of pigs among the 4 treatments (P = 0.328). However, pigs fed the PC diet (1.642) and 0.25% PRO-supplemented diet (1.641) had a lower feed to gain ratio (P < 0.05) than those fed the NC diet (1.808). The diarrhea incidence of pigs fed the 0.25% PRO-supplemented diet (7.36%) was lower (P < 0.01) than that of pigs fed the PC diet (11.61%), NC diet (16.70%), and 0.5% PRO-supplemented diet (10.08%). The frequency of antibiotic treatment of pigs consuming the 0.25% PRO-supplemented diet (4.67%) was lower (P < 0.01) than that of pigs consuming the PC diet (7.33%), NC diet (9.53%) and 0.5% PRO-supplemented diet (7.65%). No differences were found in the number of fecal E. coli among the 4 treatments. In brief, 0.25% PRO added to a nursery pig diet would be considered a potential alternative to the use of antibiotics in feed, but further research on this aspect is needed

scholarly journals Selected Lactic Acid Bacteria Synthesize Antioxidant Peptides during Sourdough Fermentation of Cereal Flours

2011 ◽  
Vol 78 (4) ◽  
pp. 1087-1096 ◽  
Author(s):  
Rossana Coda ◽  
Carlo Giuseppe Rizzello ◽  
Daniela Pinto ◽  
Marco Gobbetti
Keyword(s):  
Antioxidant Activity ◽  
Lactic Acid ◽  
Liquid Chromatography ◽  
Lactic Acid Bacteria ◽  
Ex Vivo ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Peptides ◽  
Almost All

ABSTRACTA pool of selected lactic acid bacteria was used for the sourdough fermentation of various cereal flours with the aim of synthesizing antioxidant peptides. The radical-scavenging activity of water/salt-soluble extracts (WSE) from sourdoughs was significantly (P< 0.05) higher than that of chemically acidified doughs. The highest activity was found for whole wheat, spelt, rye, and kamut sourdoughs. Almost the same results were found for the inhibition of linoleic acid autoxidation. WSE were subjected to reverse-phase fast protein liquid chromatography. Thirty-seven fractions were collected and assayedin vitro. The most active fractions were resistant to further hydrolysis by digestive enzymes. Twenty-five peptides of 8 to 57 amino acid residues were identified by nano-liquid chromatography-electrospray ionization-tandem mass spectrometry. Almost all of the sequences shared compositional features which are typical of antioxidant peptides. All of the purified fractions showedex vivoantioxidant activity on mouse fibroblasts artificially subjected to oxidative stress. This study demonstrates the capacity of sourdough lactic acid bacteria to release peptides with antioxidant activity through the proteolysis of native cereal proteins.

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