scholarly journals Application of Non-Destructive Methods: Biomarker Assays in Blood of White Stork (Ciconia ciconia) Nestlings

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2341
Author(s):  
Dora Bjedov ◽  
Alma Mikuška ◽  
Carina Lackmann ◽  
Lidija Begović ◽  
Tibor Mikuška ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Mitochondrial Fraction ◽  
Quantitative Information ◽  
White Stork ◽  
Glutathione S Transferase ◽  
Ciconia Ciconia ◽  
Surrounding Environment ◽  
Lower Variability ◽  
Non Destructive

White stork (Ciconia ciconia) nestlings can provide quantitative information on the quality of the surrounding environment by indicating the presence of pollutants, as they depend on locally foraged food. This study represents the first comparison of biomarkers in two fractions of white stork nestling blood: plasma and S9 (the post-mitochondrial fraction). The aim of this study was to evaluate acetylcholinesterase (AChE), carboxylesterase (CES), glutathione S-transferase (GST), and glutathione reductase (GR), as well as to establish a novel fluorescence-based method for glutathione (GSH) and reactive oxygen species (ROS) detection in plasma and S9. Considering the enzymatic biomarkers, lower variability in plasma was detected only for AChE, as CES, GST, and GR had lower variability in S9. Enzyme activity was higher in plasma for AChE, CES, and GST, while GR had higher activity in S9. Regarding the fluorescence-based method, lower variability was detected in plasma for GSH and ROS, although higher GSH detection was reported in S9, and higher ROS was detected in plasma. The present study indicated valuable differences by successfully establishing protocols for biomarker measurement in plasma and S9 based on variability, enzyme activity, and fluorescence. For a better understanding of the environmental effects on nestlings’ physiological condition, biomarkers can be measured in plasma and S9.

scholarly journals Insects in the White Stork Ciconia ciconia diet as indicators of its feeding conditions: the first diet study in Slovenia

Acrocephalus ◽  
2009 ◽  
Vol 30 (140) ◽  
pp. 25-29 ◽  
Author(s):  
Al Vrezec
Keyword(s):  
Breeding Success ◽  
Preliminary Test ◽  
White Stork ◽  
Ciconia Ciconia ◽  
Habitat Analysis ◽  
White Storks ◽  
Diet Study ◽  
Carrion Beetles ◽  
Bird Foraging

Insects in the White StorkCiconia ciconiadiet as indicators of its feeding conditions: the first diet study in SloveniaPrey remains found in the pellets of the White StorkCiconia ciconiaare known not to reveal the actual prey intake by the White Storks. The exceptions are the chitinous remains of insects that are quite well preserved in the pellets, and thus potentially good indicators of the actual intake of White Storks. Since insects are selected by White Storks in proportion to their abundance in the environment, they can be used as indicators of the quality of the bird foraging habitat. A preliminary test of this was carried out at three nests in NE Slovenia differing in their breeding success in 1997. In contrast to habitat analysis around the nest the proportions of Orthoptera and Coleoptera in the pellets corresponded well to the breeding success. Orthoptera species were dominant in high success, and Coleoptera (especially Carrion beetles Silphidae) in low success nests. In this paper the value of insects in White Stork pellets as indicators of the quality of the bird foraging microhabitat patches is set out. However, the indicative power of these insects needs to be tested further.

White Stork (Ciconia ciconia)

2020 ◽  
Author(s):  
Andrew Elliott ◽  
Ernest Garcia ◽  
Peter F. D. Boesman
Keyword(s):  
White Stork ◽  
Ciconia Ciconia

scholarly journals Effect of Methyl Jasmonate, Cytokinin, and Lavender Oil on Antioxidant Enzyme System of Apricot Fruit (Prunus armeniaca L.)

2021 ◽  
Vol 13 (15) ◽  
pp. 8565
Author(s):  
Seyda Cavusoglu ◽  
Nurettin Yilmaz ◽  
Firat Islek ◽  
Onur Tekin ◽  
Halil Ibrahim Sagbas ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Methyl Jasmonate ◽  
Prunus Armeniaca ◽  
Lavender Oil ◽  
Defense Systems ◽  
Antioxidant Enzyme System ◽  
Prunus Armeniaca L ◽  
Antioxidant Defense Systems ◽  
And Storage

Various treatments are carried out in order to extend both the shelf life and storage life of fresh fruit and vegetables after harvest and among them non-toxic for humans, environmentally and economically friendly alternative treatments are gained more importance. In the current study, methyl jasmonate (MeJA), cytokinin, and lavender oil which are eco-friendly and safe for human health were applied on apricot fruit. The treated fruit were stored at 0 °C and 90–95% relative humidity for 25 days and catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) enzyme activities and lipid peroxidation of apricots after treatments were studied. According to the findings obtained from the study, it was observed that 5 ppm cytokinin and 1000 ppm lavender oil treatments of apricot fruit gave better APX and CAT enzyme activity, respectively. In addition, better SOD enzyme activity in fruit was obtained with MeJA+lavender oil treatments. As a result, it can be emphasized that the product quality of apricot fruit is preserved as both the eco-friendly application of MeJA, cytokinin, and lavender oil separately from each other and the treatment of combinations between these compounds activate the enzymatic antioxidant defense systems of apricot fruit after harvest.

scholarly journals Porosity Analysis of Additive Manufactured Parts Using CAQ Technology

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1142
Author(s):  
Peter Pokorný ◽  
Štefan Václav ◽  
Jana Petru ◽  
Michaela Kritikos
Keyword(s):  
Manufacturing Process ◽  
Scanning Speed ◽  
Additive Technologies ◽  
Integration Time ◽  
Detector Resolution ◽  
Porosity Analysis ◽  
3D Scans ◽  
Printing Direction ◽  
Non Destructive

Components produced by additive technology are implemented in various spheres of industry, such as automotive or aerospace. This manufacturing process can lead to making highly optimized parts. There is not enough information about the quality of the parts produced by additive technologies, especially those made from metal powder. The research in this article deals with the porosity of components produced by additive technologies. The components used for the research were manufactured by the selective laser melting (SLM) method. The shape of these components is the same as the shape used for the tensile test. The investigated parts were printed with orientation in two directions, Z and XZ with respect to the machine platform. The printing strategy was “stripe”. The material used for printing of the parts was SS 316L-0407. The printing parameters were laser power of 200 W, scanning speed of 650 mm/s, and the thickness of the layer was 50 µm. A non-destructive method was used for the components’ porosity evaluation. The scanning was performed by CT machine METROTOM 1500. The radiation parameters used for getting 3D scans were voltage 180 kV, current 900 µA, detector resolution 1024 × 1024 px, voxel size 119.43 µm, number of projections 1050, and integration time 2000 ms. This entire measurement process responds to the computer aided quality (CAQ) technology. VG studio MAX 3.0 software was used to evaluate the obtained data. The porosity of the parts with Z and XZ orientation was also evaluated for parts’ thicknesses of 1, 2, and 3 mm, respectively. It has been proven by this experimental investigation that the printing direction of the part in the additive manufacturing process under question affects its porosity.

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