scholarly journals Exploring the Multimodal Role of Yucca schidigera Extract in Protection against Chronic Ammonia Exposure Targeting: Growth, Metabolic, Stress and Inflammatory Responses in Nile Tilapia (Oreochromis niloticus L.)

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2072
Author(s):  
Zizy I. Elbialy ◽  
Abdallah S. Salah ◽  
Ahmed Elsheshtawy ◽  
Merna Rizk ◽  
Muyassar H. Abualreesh ◽  
...  

Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its control, ammonia intoxication remains one of the most critical issues to overcome. In this study, we explored the modulatory effect and potential mechanism by which Yucca schidigera extract (YSE) can ameliorate ammonia intoxication-induced adverse effects on tilapia health and metabolism. A total number of 120 Nile tilapia were evenly assigned into four groups with three replicates each. The first group served as normal control group; the second group was exposed to ammonia alone from the beginning of the experiment and for four weeks. The third group was supplied with YSE in water at a dose of 8 mg/L and exposed to ammonia. The fourth group was supplied with YSE only in water at a dose of 8 mg/L. YSE supplementation succeeded in improving water quality by reducing pH and ammonia levels. Moreover, YSE supplementation markedly alleviated chronic ammonia-induced adverse impacts on fish growth by increasing the final body weight (FBW), specific growth rate (SGR), feed intake and protein efficiency ratio (PER) while reducing the feed conversion ratio (FCR) via improvements in food intake, elevation of hepatic insulin-like growth factor (ILGF-1) and suppression of myostatin (MSTN) expression levels with the restoration of lipid reserves and the activation of lipogenic potential in adipose tissue as demonstrated by changes in the circulating metabolite levels. In addition, the levels of hepato-renal injury biomarkers were restored, hepatic lipid peroxidation was inhibited and the levels of hepatic antioxidant biomarkers were enhanced. Therefore, the current study suggests that YSE supplementation exerted an ameliorative role against chronic ammonia-induced oxidative stress and toxic effects due to its free radical-scavenging potential, potent antioxidant activities and anti-inflammatory effects.

2021 ◽  
pp. 28-38
Author(s):  
Henry Bulama ◽  
Daniel Dahiru ◽  
Joshua Madu

Background: Cataract is a major cause of visual impairment and blindness around the world. This study evaluated the in vitro antioxidant and anti-cataract activities of Cnidoscolus aconitifolius leaves extract and fractions. Antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiozoline-6-sulfonic acid) (ABTS), total reducing power, and hydrogen peroxide scavenging assays. Anti-cataract potential was evaluated in vitro using goat lenses divided into eight groups of different treatments and incubated in artificial aqueous humor at 37 °C for 72 hours. Glucose-induced opacity in the lenses was observed and biochemical indices quantified (catalase, Malondialdehyde (MDA) and total protein in the lens homogenate). Results: The crude extract and its fractions possess substantial antioxidant activities. The aqueous fraction exhibited the best DPPH radical scavenging activity (IC50 value 78.599 µg/ml); while the dichloromethane fraction exhibited the highest ABTS radical scavenging activity with IC50 66.68 µg/ml. The anti-cataract evaluation of crude and fractions at 250 μg/ml showed a significant increase (p<0.05) in the total protein and catalase activity compared to the cataract control group. The malonaldehyde level decreased significantly (p<0.05) in all the treated groups.


2013 ◽  
Vol 42 (1) ◽  
pp. 40-43 ◽  
Author(s):  
S Yesmin ◽  
ME Uddin ◽  
R Chacrabati ◽  
M Al-Mamun

The present study was conducted to evaluate the effect of different levels of methionine supplementation on feed intake, nutrient digestibility and growth performance of growing rabbit. Sixteen weaned crossbred New Zealand White (NZW) growing rabbits (30-35 d) were distributed into four treatment groups having four replications in each group using a Completely Randomized Design (CRD). Basal diet composed of green grass (dhal grass) and concentrate mixture which was offered ad libitum basis for 56 days period. Four levels of methionine such as 0.0% (control), 0.15%, 0.25%, and 0.35% were supplemented randomly to rabbits. Results showed that supplementation of methionine did not affect green grass intake. Cumulative as well as daily concentrate and DM intake were significantly (p<0.05) higher for all methionine groups than control group. Final body weight gain as well as daily, weekly and cumulative body weight gains were improved significantly with increasing level of methionine. It was found that methionine had significant (p<0.01) effect on digestibility of DM, CP, NFE and EE but CF digestibility did not differ significantly. Digestibility was improved with increasing the level of methionine. Feed conversion ratio also decreased significantly with methionine supplementation, and 0.25% methionine group showed the best performance among the four treatments. DOI: http://dx.doi.org/10.3329/bjas.v42i1.15777 Bang. J. Anim. Sci. 2013. 42 (1): 40 43


2017 ◽  
Vol 25 (3) ◽  
pp. 201-207 ◽  
Author(s):  
Zdzisław Zakęś ◽  
Maciej Rożyński ◽  
Elżbieta Ziomek ◽  
Krystyna Demska-Zakęś

Abstract The aim of the study was to determine the impact of intramuscular tagging with passive integrated transponders (PIT tags) on the basic condition and hematological and biochemical indexes of the blood plasma of juvenile European perch (initial body weight of approximately 80 g). Throughout the observation period, i.e., for 42 days following PIT implantation, the procedure was not noted to have had a negative impact on fish growth, condition, or feed conversion ratio. The blood plasma biochemical indicators analyzed did not differ statistically significantly between the fish tagged with PIT and the untagged control group. No significant impact from this procedure was noted in the hematological indexes tested. Only with regard to the mean corpuscular hemoglobin (MCH) parameter was the value significantly lower in the fish tagged with PIT than in the control group (P ≤ 0.05). The difference determined was 1.40 pg (28.78 vs. 30.18). The MCH level in the fish tagged with PIT most probably was within the norm for this species. High tag retention (100%) and the lack of any significant impact on condition or hematological and biochemical indexes (with the exception of MCH) permits recommending this tagging method for use in juvenile perch.


2021 ◽  
Vol 50 (1) ◽  
pp. 64-72
Author(s):  
KMS Islam ◽  
MR Debi ◽  
R Haque ◽  
SN Moury

Feed of commercial broiler fortified by the external sources of vitamin and minerals which is expensive and increase the cost of feed. Blue green algae (Spirulinaplatensis) would be an alternative to those synthetic micronutrients after production in laboratory scale was studied in broiler.Spirulinaplatensiswas cultured in inorganic media contained macronutrients NaHCO3, K2HPO4, NaNO3, K2SO4, NaCl, MgSO4.7H2O, CaCl2, and FeSO4.2H2O and micronutrient solution containing H3BO4, MnCl2.4H2O, ZnSO4.7H2O, CuSO4.5H2O, MoO3, CoCl2.6H2O. Media was autoclaved and Spirulinawas cultured for 12 hours lighting with continuous aeration for 15 days to use in poultry feed. Day old broiler chick (180) was reared offering starter mash diet for 7 days. Birds were divided into 6 groups having 30 birds in each (10 per cage). Birds were offered 1. Control diet, 2. 75% premix+25% Spirulina, 3. 50% premix+50% Spirulina, 4. 25% premix+75% Spirulina, 5. 0% premix+100% Spirulina and 6. 0% premix+0% Spirulina (negative control). Spirulinawas calculated on DM basis added minerals in the media. After 21 days (28 day old) final body weight was 1039, 1070, 1044, 1065, 1117 and 893 g/bird in group 1, 2, 3, 4, 5 and 6 where higher (P<0.5) weight observed in Spirulinagroup (5). Feed intake was observed more or less similar in all groups (1483±24g). Feed conversion ratio was 1.72, 1.65, 1.69, 1.62, 1.61 and 1.99 in group 1, 2, 3, 4, 5 and 6 respectively (P<0.5) where higher in negative control group (6) and control group (1), but was lowest in 75% Spirulinagroup (4) and highest (100%) Spirulina group (5). Ash% is also highest in 100% Spirulina group 5 (55.07) than any other group. Considering growth performance of broiler, complete replacement of vitamin-mineral premix could be possible by cultured Spirulinaalong with media in broiler diet. Bang. J. Anim. Sci. 2021. 50 (1):64-72


2018 ◽  
Vol 2 ◽  
pp. 39
Author(s):  
Shahinur Nahar Moury ◽  
Md. Touhiduzzaman Sarker ◽  
Adhita Sri Prabakusuma ◽  
Md. Imrul Hasan Russel ◽  
Md. Shahidul Islam

The main purpose of this study was to explore the effects of Spirulina (Spirulina platensis) as a feed supplement by replacing Vitamin-Mineral Premix on the performance of broiler. The study explored that final body weight was 1039, 1070, 1044, 1065, 1117 and 893 g/bird in group 1, 2, 3, 4, 5 and 6 where significantly (p<0.5) higher weight observed in Spirulina group (5) but lower in negative control group (6). Feed intake observed more or less similar in all groups (1483±24g; p>0.5). Feed conversion ratio (kg FI/kg LWG) observed significantly different like 1.72, 1.65, 1.69, 1.62, 1.61 and 1.99 in group 1, 2, 3, 4, 5 and 6 respectively (p<0.5) where higher in negative group (6) and control group (1), but is lowest and similar in 75% Spirulina group (4) and highest (100%) Spirulina group (5). %Ash of different dietary groups were 46.35, 46.54, 48.93, 49.93, 55.07 and 46.92 of group 1, 2, 3, 4, 5 and 6 respectively. The %ash of bone was gradually increased among dietary groups and the best result was obtained in 100% Spirulina group 5 (55.07). It also revealed that diets containing no vitamin-mineral premix with 100% Spirulina improve the performance of broiler. The findings suggest that poultry farmer can use spirulina as a feed item for broiler for more yield.


Author(s):  
İsmail Türker ◽  
Mehmet Akif Boz ◽  
Hulusi Ozan Taşkesen ◽  
Serdar Kamanlı

This research was conducted for 42 days, in order to determine the effects of fluctuate lighting program on production and carcass characteristics of broiler chickens. 300 day old Ross 308 chicks were used. Chicks were randomly distributed to 2 lighting groups which were normal lighting (control) and fluctuate lighting, and each group was divided in to 3 replicates containing 50 chicks. For both groups lighting was arranged as 23 hours of lighting versus 1 hour of dimming. Control group was subjected to constant lighting of 20 lux/m2 for the days 0-21, and 5 lux/m2 for the days 22-42; while in the fluctuate lighting group was subjected to lighting of 5 lux/m2 for 45 minutes and additional 20 lux/m2 for 15 minutes every hour. There were no differences of final body weight, weekly body weight gain, cumulative feed intake, cumulative feed conversion ratio, viability, foot and leg problems, hot and cool carcass weight, thigh weight, breast weight, wing weight, back weight, liver or heart weights. However, there were statistically significant differences of gizzard weight and abdominal fat weight between two groups. In conclusion, gizzard and abdominal fat weights of broilers subjected to fluctuate lighting are less than control. This suggests that fluctuate lighting can be used to lower abdominal fat which is an important problem in broilers.


Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 426 ◽  
Author(s):  
Hamada A. M. Elwan ◽  
Dawood Hosni Dawood ◽  
Sally Mohamed Abd El-Aziz El-Shafei ◽  
Atef Abd El-Mohsen Abd El-Rahman ◽  
Shaker A. Abdel-Latif ◽  
...  

The current study examined the influence of Citrus limon (dry lemon) on the hemato-biochemical profiles, and antioxidant indices of growing rabbits. Forty-eight growing New Zealand White rabbits (age, eight weeks; weight, 1543.33 ± 25 g) were allocated into three groups (16 animals each), the first group was (control) fed a basal diet, whereas the second and third groups were supplemented with dried lemon, 1% or 2% DLP, respectively. A GC-MS analysis of more than 27 active constituents was performed. Feed conversion efficiency was (p < 0.05) better with diets containing 1% or 2% dry lemon, compared to the control group. Hematological indexes were increased significantly with the addition of DLP compared to those in the control group. Adding 1% or 2% dry lemon to rabbit diet increased (p < 0.05) enzymatic and non-enzymatic antioxidant activities (TAC, SOD, GSH, GST, and CAT) in serum and liver tissues. Taken together, these data reveal the advantages and antioxidant effects of dry lemon supplementation for growing rabbits once supplemented at a maximum of 2% in their daily diet.


2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S159-S162 ◽  
Author(s):  
I. Nagamine ◽  
M. Fujita ◽  
I. Hongo ◽  
H. T T Nguyen ◽  
M. Miyahara ◽  
...  

Treatment with the water and tropical lemon juice extract powders from acerola fruit purees and leaves (100 mg/kg) significantly ameliorates the hepatic inflammatory responses such as increased serum levels of AST, ALT, and GGT in rats subjected to acute D-galactosamine (GalN) intoxication. The protective effects of their constituents could be related to their antioxidant activities to neutralise free radicals to attenuate hepatic lipid peroxidation and thus can protect liver damage. The effect of the water extract powder from fruit purees (100 mg/kg) was moderately stronger than that of ascorbic acid (10 mg/kg), but weaker than that of cyanidin-3-O-rhamnoglucoside (13.3 mg/kg). The water and lemon juice extract powders from Acerola fruit purees possess the 18.6 and 24.1-fold higher DPPH radical scavenging activities, respectively, than those from leaves, the higher so for those extracted with lemon juice than for those extracted with water. The vitamin C contents were much more higher in the extract powders from fruit purees compared with those from leaves. γ-Tocopherol predominated in the extract powders from fruit purees and α-tocopherol in those from leaves. Polyphenolic compounds were identified and analysed by GC/MS-SIM after acid hydrolysis, extraction and derivatisation to trimethylsilyl ethers.


Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3046
Author(s):  
Yinyin Chen ◽  
Xiaoxiao Gong ◽  
Tianyu Yang ◽  
Maocheng Jiang ◽  
Lin Wang ◽  
...  

Sixty castrated male Haimen white growing goats with an initial age of 100 days old and similar body weight (16 ± 1.5 kg) were selected and randomly allocated into five groups with three replicates in each group with four goats in each pen (5 m × 3.2 m). Goats in the control group (CG) were fed a basal pelleted total mixed ration supplemented with 30% alfalfa hay pellet, while experimental treatments (G6, G12, G18, or G24) were supplemented with four levels (6%, 12%, 18%, or 24%) of GBLR replacing alfalfa hay pellet in the diet, separately. Results showed that (1) the final body weight, average daily gain, and average feed intake of G18 was significantly higher (p < 0.05) than CG; in contrast, the feed conversion ratio of G18 was significantly lower than CG and G12 (p < 0.05); the feed cost per head per day of CG was significantly higher (p < 0.05) than that of G18 and G24, and exhibited obvious linearly decrease (p = 0.04) with increasing GBLR supplementation; and apparent total-tract digestibility of DM and NDF in GBLR treatments were significantly higher (p < 0.05) than CG; (2) alanine transaminase (ALT) concentration in the G18 group was significantly lower (p < 0.05) than those in the control group; aspartate transaminase (AST) concentration in the G24 was significantly lower (p < 0.05) than those in the control group, and an increase in dietary level of GBLR tended to result in a linear decrease (p = 0.09) in the concentration of serum AST; (3) the concentration of malondialdehyde (MDA) demonstrated a tendency to decrease (p = 0.06) linearly with increasing GBLR supplementation; however, glutathione peroxidase (GSH-PX) activity in G12 was significantly higher (p < 0.05) than CG, G6, and G24; in addition, superoxide dismutase (SOD) activity in G18 was significantly higher (p < 0.05) than CG and G6; concentration of immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA) were not affected by GBLR, but increasing dietary GBLR showed a tendency (p = 0.08) to linearly increase the IgG concentration; the content of interleukin 4 (IL4) was significantly higher (p < 0.05) in G12, G18, and G24 than that in CG and G6; (4) There were similar NH3-N, pH, TVFA, and butyrate for goats fed different levels of GBLR supplementation; the C2 (p = 0.07) and acetate: propionate (p = 0.06) demonstrated a tendency to increase linearly with increasing level of GBLR supplementation, separately; however, it was observed that concentration of propionate showed a tendency to decrease (p = 0.08) linearly in response to GBLR supplementation; and (5) Increasing dietary GBLR tended to linearly enhance the lightness (L*) (p = 0.07) and yellowness (b*) (p = 0.09) values of longissimus dorsi muscles; the redness (a*) value in G18 was significantly higher than that in CG (p < 0.05).


Author(s):  
Sana Yagoub Abdallah Tahir ◽  
Abdalla Gadain Alnaiem ◽  
Egbal Osman Ahmed

This study was conducted at Gezira Irrigation canal to evaluate the growth performance and the effect of water tempetrature to the culture ability of Nile tilapia juveniles for 120 days. Monosex Oreochromis niloticus sized 8.17±0.33 g were carried out from Mabroka fish farm and stocked in 400 m³ fixed cages at a density of 50 fish/m³. Fish were fed daily with a commercial pellet diet with 33% protein concentration at a feeding rate of 3% of their body weight per day. Feed was given twice a day (09:00 morning and 16:00 evening). Fish growth parameters, water quality analysis have measured every two weeks. The final body weight of fish was 40.23±1.04 grams and the mean daily gained weight was 0.29 g/day, while the survival rate of fish was 99.7%±6.7 and the Food Conversion Ratio (FCR) was 3.8. The average value of temperature, pH and transparency were 23°C, 7.5, 31.5 cm respectively. The results of the study revealed that there is a possibility to culture tilapia juvenile at stocking density of 50/m³ in fixed cages in Irrigation Canals, for the effect of water temperature on fish growth performance the results obtained that no more effect on fish growth during study period.


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