scholarly journals A Comparison of Oxidative Stress Biomarkers in the Serum of Healthy Polish Dairy Goats with Those Naturally Infected with Small Ruminant Lentivirus in the Course of Lactation

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1945
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko ◽  
Michał Czopowicz ◽  
Jacek Sikora ◽  
Daria M. Urbańska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Small Ruminant ◽  
Natural Infection ◽  
Antioxidant Defenses ◽  
Lactation Period ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Modified Proteins ◽  
Oxidatively Modified ◽  
Oxidatively Modified Proteins

The present study examines the effects of natural infection by small ruminant lentivirus (SRLV) in the two most common goat breeds in Poland, i.e., Polish white improved and polish fawn improved. It focuses on biomarkers of oxidative stress, oxidatively modified proteins and antioxidant defenses, ceruloplasmin level as an acute phase protein, and the activities of antioxidant enzymes in the goat serum. It was conducted on 24 goats divided into two equal groups: one SRLV-seropositive (SRLV-SP) and another SRLV-seronegative (SRLV-SN). Both groups were identical in terms of breed and parity. Despite infection, the SRLV-SP goats demonstrated no symptoms of caprine arthritis-encephalitis. In addition, the SRLV-SP goats did not reveal pronounced dysfunctions in oxidative stress biomarkers in the serum compared to the SRLV-SN animals. However, both groups demonstrated elevated levels of the aldehydic and ketonic derivatives of oxidatively modified proteins during the lactation period. In addition, both groups retained a high total antioxidant capacity in serum despite the decrease of enzyme antioxidant defenses throughout the 200-day lactation period.

Oxidatively Modified Serotransferrin in Refractory Anemia with Ring Sideroblasts

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5502-5502
Author(s):  
Alzbeta Hlavackova ◽  
Jana Stikarova ◽  
Jiri Suttnar ◽  
Roman Kotlin ◽  
Pavel Majek ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Czech Republic ◽  
Low Risk ◽  
Refractory Anemia ◽  
Healthy Controls ◽  
Protein Levels ◽  
Ring Sideroblasts ◽  
Modified Proteins ◽  
Oxidatively Modified ◽  
Oxidatively Modified Proteins

Abstract Introduction Refractory anemia with ring sideroblasts (RARS), as a form of acquired primary sideroblastic anemia, represents about 11% of myelodysplastic syndromes (MDS) and is classified as a low risk MDS. It´s defined by the WHO as a pure dyserythropoietic disorder with presence of >15% ringed sideroblasts in the bone marrow. Abnormal expression of several genes of heme synthesis in this MDS subgroup and excessive accumulation of iron especially in mitochondria, the main place of reactive oxygen species (ROS) formation, contributes to the elevated oxidative stress. Oxidative stress is also known as one of the factors involved in the pathogenesis of MDS. High iron concentrations catalyse a Fenton reaction, where a hydroxyl radical is produced from hydrogen peroxide and causes an increase in ROS which may lead to the oxidation of DNA, lipids, and proteins, thereby causing cell damage. The aim of this study was to find a useful method for detection and identification of oxidatively modified proteins in plasma unique for RARS patients. Methods Carbonylated protein levels were determined spectrophotometrically using dinitrophenylhydrazine (DNPH) derivatization. Oxidatively modified proteins of plasma samples were derivatized with biotin hydrazide. The dialyzed biotin hydrazide labeled samples and negative controls were mixed with monomeric avidin resin. Captured carbonylated proteins were digested by trypsin and then identified by MS/MS mass spectrometry coupled to a nano-LC system. Mascot (Matrix Science, London, UK) was used for database searching (Swiss-Prot). Two unique peptides (with a higher Mascot score than the minimum for identification, P<0.05) were necessary to successfully identify a protein. Serum iron (Fe), serum ferritin, transferrin, total iron binding capacity (TIBC), and iron saturation were estimated in MDS patients in the Central National Biochemical Laboratory at the Institute of Hematology and Blood Transfusion. Results We have compared plasma of RARS patients with healthy controls or with RCMD patients. We have found significant differences in the measured carbonyl levels between all three groups (***P=0.00036). Furthermore, carbonylated protein levels were significantly elevated in RARS patients (n=10; 2.63±0.58 nmol/mg protein) compared to healthy donors (n=20; 1.80±0.42 nmol/mg protein) (***P<0.001) and to RCMD patients (n=10; 1.83±0.58 nmol/mg protein) (**P=0.00298). We have identified a total number of 27 carbonylated proteins unique for RARS patients which were generated by the effect of ROS. Serotransferrin was found as one of the oxidatively modified proteins. We have also found a significant decrease in TIBC in RARS patients compared to RCMD patients (*P=0.03078). TIBC moderately negatively correlated with carbonyl levels (r=-0.56, P=0.04864) in two investigated subgroups of MDS. Conclusions We have shown that there is a clear difference in the effect of oxidative stress between RARS, RCMD patients and healthy controls. Moreover, RARS patients, as a low risk MDS, are characterized by significantly higher protein carbonyl levels in comparison to healthy controls and to RCMD patients. The various sensibility of proteins to oxidation (carbonylation) depends both on their plasma concentration and on their susceptibility to oxidative stress as metal-binding sites or structural characteristics of the proteins. Modification in molecular structure of transferrin could be associated with decreased TIBC. This is in agreement with our data of oxidative modification of serotransferrin in RARS patients and could explain the decreased TIBC levels. Our results suggest that measurement of plasma carbonyl levels and the isolation and identification of carbonylated plasma proteins could serve as a potential diagnostic and prognostic tool in MDS. Acknowledgment This work was supported by the project of the Ministry of Health of the Czech Republic for conceptual development of the research organization 00023736, by Grant from the Academy of Sciences, Czech Republic (P205/12/G118), and by ERDF OPPK CZ.2.16/3.1.00/28007. Disclosures No relevant conflicts of interest to declare.

scholarly journals Habitat-, age-, and sex-related alterations in oxidative stress biomarkers in the blood of mute swans (Cygnus olor) inhabiting pomeranian coastal areas (Northern Poland)

2021 ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko
Keyword(s):  
Coastal Areas ◽  
Mute Swan ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Cygnus Olor ◽  
Northern Poland ◽  
Total Antioxidant ◽  
Oxidatively Modified ◽  
Mute Swans ◽  
Oxidatively Modified Proteins

AbstractThe mute swan (Cygnus olor) can be considered a representative species of birds associated with the aquatic environment and responding very clearly to changes in the environment. Assuming that the condition of the mute swan population well reflects the state of the environment, this species was used in our research as a bioindicative species. Thus, the aim of our study was to elucidate the association between metal contents in soil samples collected from a habitat of mute swans and element contents in their feathers as well as the levels of biomarkers of lipid peroxidation, oxidatively modified proteins, and total antioxidant capacity in the blood of mute swans living in three agglomerations in coastal areas in the southern part of the Baltic Sea (Pomeranian region, northern Poland). We compared the effects of inhabitation, age, and sex on the ecophysiological accumulation of metals in three wintering populations of the mute swan from coastal areas of northern Poland, i.e., Słupsk, Gdynia, and Sopot. In Słupsk, the anthropogenic pressure was related predominantly to the level of Al and, to a lesser extent, to the content of Rh and Ru. We found maximum levels of lipid peroxidation biomarkers in the blood of the mute swans from Gdynia (38.20 ± 6.35 nmol MDA·mL−1). At the same time, maximum levels of aldehydic and ketonic derivatives of oxidatively modified proteins were noted in the blood of swans from Sopot compared to the values obtained in mute swans from Słupsk and Gdynia. This trend suggesting high levels of oxidative stress biomarkers was also confirmed by a decrease in the total antioxidant capacity in these groups.

scholarly journals EXERCISE-INDUCED ALTERATIONS OF THE OXIDATIVE STRESS BIOMARKERS IN ERYTHROCYTESOF PONIES INVOLVED IN RECREATIONAL HORSEBACK RIDING

2020 ◽  
pp. 39-48
Author(s):  
Halyna Tkachenko ◽  
Natalia Kurhaluk ◽  
Iryna Tkachova
Keyword(s):  
Oxidative Stress ◽  
Training Session ◽  
Rest Period ◽  
Antioxidant Defenses ◽  
Exercise Session ◽  
Horseback Riding ◽  
Oxidative Stress Biomarkers ◽  
Jugular Veins ◽  
Erythrocyte Hemolysis ◽  
Stress Biomarkers

The aim of the current study was the analysis of the oxidative stress biomarkers, as well as the osmotic-induced resistance of erythrocytes in mares and stallions of ponies involved in recreational horseback riding in Pomeranian regions. Ten healthy adult Hucul ponies (5 stallions and 5 mares), 5-11 years old, from Pomeranian regions in Poland (Ustka city, Pomeranian Voivodship, Poland) were used in our study. All horses participated in recreational horseback riding and were subjected to the resembling type of management. The training was continued 1 hour and included a ride of cross country by walking (10 min), trotting (15 min), walking (10 min), galloping (15 min), and walking (10 min). Blood was drawn from jugular veins of the animals in the morning, 90 minutes after feeding, and immediately after the exercise session. Marker of lipid peroxidation (2-Thiobarbituric acid reactive substances, TBARS), aldehydic and ketonic derivatives in the erythrocytes, total antioxidant capacity (TAC) level in the erythrocytes of both mares and stallions exhibited non-significant changes immediately after exercise as compared to the resting period. Both at the rest and after the training session, the levels of TBARS, aldehydic and ketonic derivatives in the erythrocyte suspensions of mares were non-significantly higher compared to stallions. In a like manner, differences of aldehydic and ketonic derivatives of oxidatively modified proteins between mares and stallions after training sessions were noted. Both at the rest and after the training session, the TAC level in the erythrocyte suspensions of stallions was non-significantly higher compared to mares. Among both mares and stallions, a non-significantly decreased erythrocyte hemolysis was observed after training sessions compared to the rest period. A comparison of erythrocyte hemolysis in mares and stallions at the rest period showed increased values of hemolysis in the stallions. After the training session, decreased hemolysis was observed in the stallions compared to mares. Efforts should be directed toward a thorough characterization of antioxidant defenses, as well as the correlation links between oxidative stress biomarkers and antioxidant defenses including age- and gender-related differences in the training programs of horses involved in the recreational horseback ridings.

scholarly journals Oxidative Stress Biomarkers in Urine of Metal Carpentry Workers Can Be Diagnostic for Occupational Exposure to Low Level of Welding Fumes from Associated Metals

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3167
Author(s):  
Flavia Buonaurio ◽  
Maria Luisa Astolfi ◽  
Daniela Pigini ◽  
Giovanna Tranfo ◽  
Silvia Canepari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Occupational Exposure ◽  
Oxidation Products ◽  
Control Group ◽  
Welding Fumes ◽  
Oxidative Stress Biomarkers ◽  
Limit Values ◽  
Stress Biomarkers ◽  
Specificity And Sensitivity ◽  
The Impact

Urinary concentrations of 16 different exposure biomarkers to metals were determined at the beginning and at the end of a working shift on a group of workers in the metal carpentry industry. Five different oxidative stress biomarkers were also measured, such as the oxidation products of RNA and DNA metabolized and excreted in the urine. The results of workers exposed to metals were compared to those of a control group. The metal concentrations found in these workers were well below the occupational exposure limit values and exceeded the mean concentrations of the same metals in the urine of the control group by a factor of four at maximum. Barium (Ba), mercury (Hg), lead (Pb) and strontium (Sr) were correlated with the RNA oxidative stress biomarker, 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), which was found able to discriminate exposed workers from controls with a high level of specificity and sensitivity. The power of this early diagnostic technique was assessed by means of the ROC curve. Ba, rubidium (Rb), Sr, tellurium (Te), and vanadium (V) were correlated with the level of the protein oxidation biomarker 3-Nitrotyrosine (3-NO2Tyr), and Ba, beryllium (Be), copper (Cu), and Rb with 5-methylcytidine (5-MeCyt), an epigenetic marker of RNA damage. These effect biomarkers can help in identifying those workers that can be defined as “occupationally exposed” even at low exposure levels, and they can provide information about the impact that such doses have on their health.

scholarly journals Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natalia Kurhaluk ◽  
Halyna Tkachenko
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Muscle Tissue ◽  
Salmo Trutta ◽  
Protein Carbonyl ◽  
Oxidative Modification ◽  
Oxidative Stress Biomarkers ◽  
Development Stages ◽  
Stress Biomarkers ◽  
Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

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