scholarly journals Genomic Epidemiology of Shiga Toxin-Producing Escherichia coli Isolated from the Livestock-Food-Human Interface in South America

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1845
Author(s):  
Nicolás Galarce ◽  
Fernando Sánchez ◽  
Beatriz Escobar ◽  
Lisette Lapierre ◽  
Javiera Cornejo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South America ◽  
Shiga Toxin ◽  
Genomic Data ◽  
Genome Sequences ◽  
Multilateral Trade ◽  
Human Interface ◽  
Genomic Epidemiology ◽  
Food Borne ◽  
Sequence Types

Shiga toxin-producing Escherichia coli (STEC) are zoonotic pathogens responsible for causing food-borne diseases in humans. While South America has the highest incidence of human STEC infections, information about the genomic characteristics of the circulating strains is scarce. The aim of this study was to analyze genomic data of STEC strains isolated in South America from cattle, beef, and humans; predicting the antibiotic resistome, serotypes, sequence types (STs), clonal complexes (CCs) and phylogenomic backgrounds. A total of 130 whole genome sequences of STEC strains were analyzed, where 39.2% were isolated from cattle, 36.9% from beef, and 23.8% from humans. The ST11 was the most predicted (20.8%) and included O-:H7 (10.8%) and O157:H7 (10%) serotypes. The successful expansion of non-O157 clones such as ST16/CC29-O111:H8 and ST21/CC29-O26:H11 is highlighted, suggesting multilateral trade and travel. Virulome analyses showed that the predominant stx subtype was stx2a (54.6%); most strains carried ehaA (96.2%), iha (91.5%) and lpfA (77.7%) genes. We present genomic data that can be used to support the surveillance of STEC strains circulating at the livestock-food-human interface in South America, in order to control the spread of critical clones “from farm to table”.

scholarly journals Whole-Genome Sequencing Applied to the Molecular Epidemiology of Shiga Toxin-Producing Escherichia coli O157:H7 in Argentina: TABLE 1 

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Claudia Carolina Carbonari ◽  
Nahuel Fittipaldi ◽  
Sarah Teatero ◽  
Taryn B. T. Athey ◽  
Luis Pianciola ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Epidemiology ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Shiga Toxin ◽  
Whole Genome ◽  
Escherichia Coli O157 ◽  
Genome Sequences ◽  
High Quality ◽  
Human Infections

Shiga toxin-producing Escherichia coli strains are worldwide associated with sporadic human infections and outbreaks. In this work, we report the availability of high-quality draft whole-genome sequences for 19 O157:H7 strains isolated in Argentina.

scholarly journals High-Quality Whole-Genome Sequences for 77 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing

2018 ◽  
Vol 6 (19) ◽  
Author(s):  
Pooja N. Patel ◽  
Rebecca L. Lindsey ◽  
Lisley Garcia-Toledo ◽  
Lori A. Rowe ◽  
Dhwani Batra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Foodborne Pathogen ◽  
Severe Illness ◽  
Whole Genome ◽  
Genome Sequences ◽  
Pacbio Sequencing ◽  
High Quality ◽  
Content Type ◽  
Sequencing Platform

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is an enteric foodborne pathogen that can cause mild to severe illness. Here, we report the availability of high-quality whole-genome sequences for 77 STEC strains generated using the PacBio sequencing platform.

scholarly journals Genome Resource of Two Potato Strains of Ralstonia solanacearum Biovar 2 (Phylotype IIB Sequevar 1) and Biovar 2T (Phylotype IIB Sequevar 25) Isolated from Lowlands in Iran

2020 ◽  
Vol 33 (7) ◽  
pp. 872-875 ◽  
Author(s):  
Nasim Sedighian ◽  
Marjon Krijger ◽  
Tanvi Taparia ◽  
S. Mohsen Taghavi ◽  
Emmanuel Wicker ◽  
...  
Keyword(s):  
South America ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Complete Genome ◽  
Genomic Data ◽  
Brown Rot ◽  
Genome Sequences ◽  
Rot Disease ◽  
Tropical Lowlands ◽  
Major Pathogens

Ralstonia solanacearum, the causal agent of bacterial wilt and brown rot disease, is one of the major pathogens of solanaceous crops, including potato, around the globe. Biovar 2T (phylotype II/sequevar 25) of R. solanacearum is adapted to tropical lowlands and is only reported in South America and Iran. Thus far, no genome resource of the biovar 2T of the pathogen has been available. Here, we present the near-complete genome sequences of the biovar 2T strain CFBP 8697 as well as strain CFBP 8695 belonging to biovar 2 race 3, both isolated from potato in Iran. The genomic data of biovar 2T will extend our understanding of the virulence features of R. solanacearum and pave the way for research on biovar 2T functional and interaction genetics.

scholarly journals Draft Genome Sequences of Five Shiga Toxin-Producing Escherichia coli Isolates Harboring the New and Recently Described Subtilase Cytotoxin Allelic Variant subAB2-3

2017 ◽  
Vol 5 (8) ◽  
Author(s):  
Taurai Tasara ◽  
Lisa Fierz ◽  
Jochen Klumpp ◽  
Herbert Schmidt ◽  
Roger Stephan
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Sequence Data ◽  
Draft Genome ◽  
Allelic Variant ◽  
Genome Sequences ◽  
Content Type ◽  
Subtilase Cytotoxin

ABSTRACT We present here the draft genome sequences of five Shiga toxin-producing Escherichia coli (STEC) strains which tested positive in a primary subAB screening. Assembly and annotation of the draft genomes revealed that all strains harbored the recently described allelic variant subAB 2-3 . Based on the sequence data, primers were designed to identify and differentiate this variant.

scholarly journals Behavior of Different Shiga Toxin-Producing Escherichia coli Serotypes in Various Experimentally Contaminated Raw-Milk Cheeses

2012 ◽  
Vol 79 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Stéphane D. Miszczycha ◽  
Frédérique Perrin ◽  
Sarah Ganet ◽  
Emmanuel Jamet ◽  
Fanny Tenenhaus-Aziza ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Human Health Risk ◽  
Raw Milk ◽  
Public Health Implication ◽  
Content Type ◽  
Growth And Survival ◽  
The Public ◽  
Food Borne ◽  
Food Borne Illness

ABSTRACTShiga toxin-producingEscherichia coli(STEC) is an important cause of food-borne illness. The public health implication of the presence of STEC in dairy products remains unclear. Knowledge of STEC behavior in cheeses would help to evaluate the human health risk. The aim of our study was to observe the growth and survival of experimentally inoculated STEC strains in raw-milk cheeses manufactured and ripened according to five technological schemes: blue-type cheese, uncooked pressed cheese with long ripening and with short ripening steps, cooked cheese, and lactic cheese. Cheeses were contaminated with different STEC serotypes (O157:H7, O26:H11, O103:H2, and O145:H28) at the milk preparation stage. STEC growth and survival were monitored on selective media during the entire manufacturing process. STEC grew (2 to 3 log10CFU · g−1) in blue-type cheese and the two uncooked pressed cheeses during the first 24 h of cheese making. Then, STEC levels progressively decreased in cheeses that were ripened for more than 6 months. In cooked cheese and in lactic cheese with a long acidic coagulation step (pH < 4.5), STEC did not grow. Their levels decreased after the cooking step in the cooked cheese and after the coagulation step in the lactic cheese, but STEC was still detectable at the end of ripening and storage. A serotype effect was found: in all cheeses studied, serotype O157:H7 grew less strongly and was less persistent than the others serotypes. This study improves knowledge of the behavior of different STEC serotypes in various raw-milk cheeses.

scholarly journals Molecular Subtyping and Distribution of the Serine Protease from Shiga Toxin-Producing Escherichia coli among Atypical Enteropathogenic E. coli Strains

2009 ◽  
Vol 75 (7) ◽  
pp. 2246-2249 ◽  
Author(s):  
Adrian L. Cookson ◽  
Jenny Bennett ◽  
Carolyn Nicol ◽  
Fiona Thomson-Carter ◽  
Graeme T. Attwood
Keyword(s):  
Escherichia Coli ◽  
Serine Protease ◽  
Shiga Toxin ◽  
Molecular Subtyping ◽  
E Coli ◽  
Sequence Types

ABSTRACT Atypical enteropathogenic Escherichia coli (aEPEC) and Shiga toxin-producing E. coli (STEC) were examined to determine the prevalence and sequence of espP, which encodes a serine protease. These analyses indicated shared espP sequence types between the two E. coli pathotypes and thus provide further insights into the evolution of aEPEC and STEC.

scholarly journals Complete Annotated Genome Sequences of Two Shiga Toxin-Producing Escherichia coli Strains and One Atypical Enteropathogenic E. coli Strain, Isolated from Naturally Colonized Cattle of German Origin

2017 ◽  
Vol 5 (19) ◽  
Author(s):  
Lutz Geue ◽  
Christian Menge ◽  
Christian Berens ◽  
Stefanie A. Barth
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Coli Strain ◽  
Enteric Pathogens ◽  
Genome Sequences ◽  
Content Type ◽  
E Coli ◽  
German Origin ◽  
Cattle Herds ◽  
German Cattle

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) are important zoonotic enteric pathogens with the main reservoir in cattle. Here, we present the genomes of two STEC strains and one atypical enteropathogenic E. coli strain from cattle origin, obtained during a longitudinal study in German cattle herds.

Draft genome sequences of two fluoroquinolone-resistant CTX-M-15-producing Escherichia coli ST90 (ST23 complex) isolated from a calf and a dairy cow in South America

2017 ◽  
Vol 11 ◽  
pp. 145-147 ◽  
Author(s):  
Luciana Sartori ◽  
Miriam R. Fernandes ◽  
Susan Ienne ◽  
Tiago A. de Souza ◽  
Lilian Gregory ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South America ◽  
Dairy Cow ◽  
Draft Genome ◽  
Genome Sequences
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