scholarly journals Overview of the Genus Boleodorus and First Reports of Boleodorus thylactus and B. volutus from Southern Alberta, Canada

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1760
Author(s):  
Maria Munawar ◽  
Dmytro P. Yevtushenko ◽  
Pablo Castillo
Keyword(s):  
Phylogenetic Trees ◽  
Agricultural Fields ◽  
Tabular Form ◽  
Ecological Studies ◽  
Morphometric Characters ◽  
Rdna Sequences ◽  
Southern Alberta ◽  
18S Rdna Sequences ◽  
Morphological And Molecular Characterization

The present study provides the morphological and molecular characterization of Boleodorus thylactus and B. volutus populations, recovered from agricultural fields of southern Alberta. Despite a significant abundance of this group of nematodes, none of the Boleodorus species were previously reported in Canada. Therefore, representative adult specimens of each population were photographed and examined morphometrically. Phylogenetic trees were reconstructed using partial D2–D3 expansion segments of the 28S and 18S rDNA sequences to understand the relationships of Boleodorus species with Tylenchidae-related genera. Boleodorus species are relevant to soil ecological studies and therefore we summarized the important morphological and morphometric characters in tabular form for easy and efficient species identification. Moreover, we discuss the associated hosts and the distribution of all described Boleodorus species. This study will serve as a guide and basic framework for species diagnostics in the genus Boleodorus and will aid in filling the gaps in our knowledge of the species present in our cultivated lands.

Evolutionary relationships between 15 Plasmodium species from New and Old World primates (including humans): a 18S rDNA cladistic analysis

Parasitology ◽  
2004 ◽  
Vol 129 (6) ◽  
pp. 677-684 ◽  
Author(s):  
M. C. LECLERC ◽  
J. P. HUGOT ◽  
P. DURAND ◽  
F. RENAUD
Keyword(s):  
18S Rdna ◽  
Phylogenetic Trees ◽  
World Monkey ◽  
Cladistic Analysis ◽  
Plasmodium Species ◽  
African Species ◽  
Old World ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
Group 3

We present a new phylogenetic analysis of 15 primate Plasmodium species based on 18S rDNA sequences including new sequences of Plasmodium coatneyi, P. fieldi, P. gonderi, P. hylobati and P. simium. The results are discussed in the context of the parasite host species and their geographical distribution. Contrary to other phylogenies constructed with this 18S rDNA molecule, we observed that the topology of phylogenetic trees was not affected either by the quality of the nucleotide matrices, or by the species present in the outgroup. This analysis showed the following. (1) The polyphyly of human Plasmodium is confirmed. (2) The monophyly of Plasmodium from Old World monkeys is confirmed by the new added sequences and P. gonderi, an African species, possibly could be at the root of this group. (3) The most parsimonious biogeographical hypothesis is that P. vivax originated in Asia; thus, its related species P. simium appears to be derived through a transfer from the human P. vivax to New World monkey species in South America. (4) Sampling efforts of non-human primate Plasmodium could permit improvement of the knowledge of primate Plasmodium phylogeny and also consideration of the risks of malaria emergence from monkey reservoirs.

scholarly journals Taxonomic characterization of ten Thraustochytrids strains isolated from mangrove Xuan Thuy, Nam Dinh

2016 ◽  
Vol 14 (2) ◽  
pp. 377-384
Author(s):  
Nguyễn Thị Hoài Hà ◽  
Phạm Thị Bích Đào ◽  
Nguyễn Đình Tuấn
Keyword(s):  
Cell Division ◽  
18S Rdna ◽  
Rdna Sequences ◽  
Marine Habitats ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Binary Division ◽  
18S Rdna Sequences ◽  
Amoeboid Cells

Thraustochytrids have become of considerable industrial and scientific interest in the past decade due to their health benefits. Thraustochytrids are found in a wide variety of marine habitats such as the coastal, mangrove and sediments including the deep sea. Thraustochytrids are extremely common on the detritus, macroalgae and decaying leaf, they play an important role as organic matter-degrading microorganisms Thraustochytrids are unicellular, eukaryotic, chemo-organotrophic organisms. Ten thraustochytrids strains PT269, PT270, PT273, PT274, PT279, PT284, PT285, PT287, PT81, PT84 were isolated from four locations in Xuan Thuy mangroves, Nam Dinh. In this report, classification is based on morphology and 18S rDNA sequences. Ten Thraustochytrid strains could be classified into three types of colony and four types of cell morphology. Molecular phylogenetic analysis of 18S rDNA sequences showed homology score to be 99-100% and these strains belonged to four genera in the family Thraustochytriaceae. PT269, PT279, PT284 and PT287 strains belong to Aurantiochytrium genus, they produce amoeboid cells and occur successive binary division. PT273 and PT285 strains belong to Thraustochytrium genus, thallus directly develop and cleave into sporangium. PT274 strain belong to Aplanochytrium genus with two distinct development, amoeboid cells are found, they rapidly round up and become sporangium; and successive binary cell division. PT270, PT81 and PT84 strains belong to genus Schizochytrium, they have successive binary cell division, zoospores release.

scholarly journals Reconstructing the Phylogeny ofCapsosiphon fulvescens(Ulotrichales, Chlorophyta) from Korea Based onrbcL and 18S rDNA Sequences

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Sang-Mi Sun ◽  
Seung Hwan Yang ◽  
Kirill S. Golokhvast ◽  
Bao Le ◽  
Gyuhwa Chung
Keyword(s):  
Phylogenetic Tree ◽  
18S Rdna ◽  
Phylogenetic Trees ◽  
Economic Value ◽  
Phylogenetic Position ◽  
Therapeutic Effects ◽  
Filamentous Green Algae ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
Taxonomic Studies

Capsosiphon fulvescensis a filamentous green algae in the class Ulvophyceae. It has been consumed as food with unique flavor and soft texture to treat stomach disorders and hangovers, and its economic value justifies studying its nutritional and potential therapeutic effects. In contrast to these applications, only a few taxonomic studies have been conducted onC. fulvescens. In particular, classification and phylogenetic relationships of theC. fulvescensbelow the order level are controversial. To determine its phylogenetic position in the class, we usedrbcL and 18S rDNA sequences as molecular markers to construct phylogenetic trees. The amplifiedrbcL and 18S rDNA sequences from 4C. fulvescensisolates (Jindo, Jangheung, Wando, and Koheung, Korea) were used for phylogenetic analysis by employing three different phylogenetic methods: neighbor joining (NJ), maximum parsimony (MP), and maximum likelihood (ML). TherbcL phylogenetic tree showed that all taxa in the order Ulvales were clustered as a monophyletic group and resolved the phylogenetic position ofC. fulvescensin the order Ulotrichales. The significance of our study is that the 18S rDNA phylogenetic tree shows the detailed taxonomic position ofC. fulvescens. In our result,C. fulvescensis inferred as a member of Ulotrichaceae, along withUrosporaandAcrosiphonia.

Rhabditis (Oscheius) colombiana n. sp. (Nematoda: Rhabditidae), a necromenic associate of the subterranean burrower bug Cyrtomenus bergi (Hemiptera: Cydnidae) from the Cauca Valley, Colombia

Nematology ◽  
2005 ◽  
Vol 7 (3) ◽  
pp. 363-373 ◽  
Author(s):  
S. Patricia Stock ◽  
Ana Caicedo ◽  
Paul Calatayud
Keyword(s):  
Life History ◽  
New Species ◽  
Life History Traits ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Total Size ◽  
Morphometric Characters ◽  
Rdna Sequences ◽  
18S Rdna Sequences ◽  
Males And Females

AbstractRhabditis (Oscheius) colombiana n. sp. is described as a necromenic associate of the burrower bug Cyrtomenus bergi (Hemiptera: Cydnidae) collected in the Cauca Valley, Colombia. The new species resembles others of the insectivora-group, viz, Rhabditis (O.) insectivora Körner, 1954 and Rhabditis (O.) lucianii Maupas, 1919, in a number of morphological and life history traits, but can be separated by a combination of morphological and morphometric characters. The most distinctive morphological features that separate R. (O.) colombiana n. sp. from these two Rhabditis species are the total size of both males and females, which are much shorter and slimmer in the new species; the size of the male spicules and the values of ratios b and c. Additionally, molecular data (18S rDNA sequences) and phylogenetic analyses demonstrated the distinctiveness of this species when compared to a number of other Rhabditis (Oscheius) species.

scholarly journals Molecular phylogeny of the kidney-parasitic Sphaerospora renicola from common carp (Cyprinus carpio) and Sphaerospora sp. from goldfish (Carassius auratus auratus)

2004 ◽  
Vol 52 (4) ◽  
pp. 469-478 ◽  
Author(s):  
Edit Eszterbauer ◽  
Cs. Székely
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Carassius Auratus ◽  
Phylogenetic Trees ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Freshwater Species ◽  
Goldfish Carassius Auratus ◽  
Rdna Sequences ◽  
18S Rdna Sequences

The genetic relatedness of two kidney-parasitic Sphaerospora species was studied. Although S. renicola, the causative agent of swimbladder inflammation of common carp fingerlings (Cyprinus carpio), and Sphaerospora sp. originating from goldfish (Carassius auratus auratus) were indistinguishable on the basis of spore morphology, they were found to be genetically different as their 18S rDNA sequences shared only 71.9% identical nucleotides. In the phylogenetic trees, Sphaerospora sp. from goldfish grouped with Myxidium truttae (AJ582061) within the clade of the coelozoic freshwater species. Sphaerospora renicola clustered with S. molnari (AF378345) within the group of myxosporeans histozoic in gills. The topology of the six Sphaerospora species on the phylogenetic trees implied that myxospore morphology does not correlate with the genetic relationships, and the genus seems to be polyphyletic.

scholarly journals Morphological and Molecular Characterization of Globodera Populations from Oregon and Idaho

2011 ◽  
Vol 101 (4) ◽  
pp. 480-491 ◽  
Author(s):  
A. M. Skantar ◽  
Z. A. Handoo ◽  
I. A. Zasada ◽  
R. E. Ingham ◽  
L. K. Carta ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Globodera Pallida ◽  
Second Stage ◽  
Rdna Sequences ◽  
Individual Bands ◽  
Stage Juvenile ◽  
Polymerase Chain ◽  
Stylet Length ◽  
Morphological And Molecular Characterization

An unusual population of cyst nematode was found in soils collected from a Powell Butte, OR field with a cropping history including potato, wheat, other crops, and significant weed presence. These nematodes could not be placed with certainty into any known species and exhibited some unique morphological features in some specimens. Compared with Globodera pallida, the cyst body length was slightly longer and the second-stage juvenile stylet length was slightly shorter. In some individuals, the J2 stylet knob height was greater and the tail annules were more prominent than in G. pallida, and the tail abruptly narrowed, with a slight constriction near the posterior third of the hyaline terminus. Compared with G. rostochiensis, the hyaline tail terminus had a larger number of refractive bodies, and cysts of this population had a smaller Granek's ratio and fewer cuticular ridges between the anus and vulva. In some individuals, the tail termini of second-stage juveniles were more bluntly pointed, and the stylet knobs were more anteriorly directed with greater height. Unlike G. tabacum, the cyst wall often lacked a network-like pattern and, in some individuals, the juvenile tail terminus distinctly narrowed after a constriction. Molecularly, the population was distinct from G. pallida, G. rostochiensis, and G. tabacum. Multiplex polymerase chain reaction of the internal transcribed spacer (ITS) rDNA region gave results similar to G. tabacum; however, ITS restriction fragment length polymorphism patterns were observed to have individual bands in common with G. rostochiensis and G. pallida. Phylogenetic analysis based on ITS1 and -2 rDNA sequences showed greatest similarity to populations from Argentina and Chile; together, they form a moderately supported clade, distinct from G. rostochiensis, G. tabacum, G. “mexicana,” European type G. pallida, and several G. pallida populations from South America.

scholarly journals Morphological and molecular characterization of Orbilia pseudopolybrocha and O. tonghaiensis, two new species of Orbiliaceae from China

2020 ◽  
Vol 70 (4) ◽  
pp. 2664-2676 ◽  
Author(s):  
Ying Zhang ◽  
Min Qiao ◽  
H.O. Baral ◽  
Jianping Xu ◽  
Ke-Qin Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Phylogenetic Relationships ◽  
Morphological Traits ◽  
Its Sequences ◽  
Rdna Sequences ◽  
Two New Species ◽  
Asexual Morphs ◽  
Morphological And Molecular Characterization

This study examined over 20 fungal specimens of the Orbiliaceae (Orbiliomycetes) from different regions in China. Our analyses based on morphological traits and the ITS rDNA sequences revealed two new Orbilia species with drechslerella-like asexual morphs. These new species are able to trap nematodes with constricting rings. In addition, Orbilia cf. orientalis is reported as a new cryptic Chinese variant of European collections of O. orientalis. All three species are described and illustrated in detail in this paper. Their phylogenetic relationships with other orbiliaceous species were identified based on their ITS sequences.

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