scholarly journals Proximal Perineural Femoral Nerve Injection in Pigs Using an Ultrasound-Guided Lateral Subiliac Approach—A Cadaveric Study

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1759
Author(s):  
Robert Trujanovic ◽  
Pablo E. Otero ◽  
Peter Marhofer ◽  
Ulrike Auer ◽  
Silvio Kau
Keyword(s):  
Methylene Blue ◽  
Pain Control ◽  
Femoral Nerve ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Ultrasound Guided ◽  
Technical Feasibility ◽  
Pelvic Limb ◽  
Psoas Major

Desensitizing the femoral nerve (FN) improves pain control in several species undergoing pelvic limb surgeries. Despite its advantages, this method has not yet been described in pigs, although they make an accepted surgical animal model. Based on anatomical dissections, first performed in two pig cadavers, an ultrasound-guided access for localization and perineural infiltration of the FN trunk at the iliopsoas compartment level was specified. The FN was found running between the psoas major and medial portion of iliac muscle. Ultrasonographically, the FN appeared as a hypoechogenic round–oval structure surrounded by a hyperechogenic rim. Technical feasibility and accuracy were assessed in six additional pig cadavers by injecting 0.15 mL kg−1 methylene blue dye bilaterally in direct proximity to the nerve. The needle was inserted caudoventral to the coxal tuber, traversing the ultrasound plane as it progressed towards the FN in dorsomedial direction. Staining of the nerve was evaluated by dissection. The injection was considered effective if the nerve was stained in its entire circumference over a length of 2 cm. FNs of all investigated individuals could be successfully stained. This ultrasound-guided subiliac approach allows feasible and accurate access to the FN and may be useful in producing a successful blockade in vivo.

scholarly journals Successful Localization of Abnormal Parathyroid Gland Using Ultrasound-Guided Methylene Blue Dye Injection in the Reoperative Neck

2014 ◽  
Vol 77 (S3) ◽  
pp. 1094-1097 ◽  
Author(s):  
Mehmet Haciyanli ◽  
Melike Bedel Koruyucu ◽  
Nezahat Karaca Erdoğan ◽  
Ozcan Dere ◽  
Erdem Sarı ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Parathyroid Gland ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Ultrasound Guided ◽  
Abnormal Parathyroid Gland

Ultrasound-Guided Methylene Blue Dye Injection for Parathyroid Localization in the Reoperative Neck

2013 ◽  
Vol 38 (1) ◽  
pp. 88-91 ◽  
Author(s):  
Leah Candell ◽  
Michael J. Campbell ◽  
Wen T. Shen ◽  
Jessica E. Gosnell ◽  
Orlo H. Clark ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Ultrasound Guided ◽  
Parathyroid Localization

scholarly journals Preoperative Axillary Ultrasound-guided Wire Localization and Lymphoscintigraphy for Sentinel Lymph Node Biopsy in Breast Cancer Patients

2021 ◽  
Author(s):  
Fezzeh Elyasinia ◽  
Homa Hemmasi ◽  
Karamollah Toolabi ◽  
Afsaneh Alikhassi ◽  
Mehran Sohrabi Maralani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Methylene Blue ◽  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Cancer Patients ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Breast Cancer Patients ◽  
Wire Localization ◽  
Ultrasound Guided

Background: Breast cancer has the highest incidence and mortality among female malignant tumors. Breast cancer with negative axillary lymph nodes has been diag- nosed mainly at an early stage. Sentinel lymph node biopsy (SLNB) is a standard screening technique for patients with early-stage breast cancer and clinically nega- tive lymph nodes. Lymphoscintigraphy (sentinel lymph node mapping) has been reg- ularly used as the standard method for SLNB. Today, ultrasound-guided wire locali- zation (USGWL) is a well-established technique with superior outcomes. Therefore, we attempted to determine whether preoperative UGWL and lymphoscintigraphy (blue dye and isotope injection) improve SLN detection and false-negative rate in breast cancer patients undergoing SLNB and identify clinical factors that may affect the diagnostic accuracy of axillary ultrasound (AUS). Methods: Between December 2018 and June 2019, 55 patients with clinical T1- 3N0 breast cancer eligible for an SLNB at Imam Khomeini Hospital in Tehran were included in our study. Tumor characteristics and demographic data were collect- ed by reviewing medical records and questionnaires prepared by our surgical team. The day before SLNB, all patients underwent ultrasound-guided wire localization of SLN. Lymphoscintigraphy was performed with an unfiltered 99mTc-labelled sulfur colloid peritumoral injection followed by methylene blue dye injection. The results were analyzed based on the permanent pathology report. Results: Among the 55 patients, 71.8% of SLNs were detected by wire localization, while 57.8% were found by methylene blue mapping and 59.6% by gamma probe detection. Compared with wire localization and isotope injection, the methylene blue dye technique had a low sensitivity (72.2%), while both wire localization and isotope injection reached 77.8%. The sensitivity, specificity, and accuracy of UGWL were 77.8%, 42.1%, and 65.4%, respectively. Otherwise, methylene blue dye and isotope injection accuracy was 47.3% and 50.1%, respectively. Furthermore, there was a significant relationship between BMI, tumor size, laterality, reactive ALN, and the accuracy of preoperative AUS. But there was no significant correlation between age, weight, height, tumor biopsy, tumor location, the time interval between methylene blue dye and isotope injection to surgery, and also the type of surgery to the accuracy of preoperative AUS. Conclusion: Preoperative UGWL can effectively identify SLNs compared to lym- phoscintigraphy (blue dye and isotope injection) in early breast cancer patients un- dergoing SLNB.

Novel ultrasound-guided lateral approach for femoral nerve block in cats: a pilot study

2019 ◽  
Vol 22 (4) ◽  
pp. 339-343
Author(s):  
Diego Rodrigo-Mocholi ◽  
Fernando Martinez-Taboada
Keyword(s):  
Methylene Blue ◽  
Nerve Block ◽  
Femoral Nerve Block ◽  
Femoral Nerve ◽  
Contralateral Side ◽  
Lateral Approach ◽  
The Novel ◽  
Ultrasound Guided ◽  
Pelvic Limb ◽  
Nerve Staining

Objectives The aim of this study was to describe the technique for a novel lateral approach to femoral nerve block in cat cadavers. Methods Five feline cadavers were used in our prospective cadaveric experimental study. Ultrasonography was used to identify the anatomy injection site. Both pelvic limbs were injected with a volume (0.1 ml/kg) of methylene blue in each cat. The cadavers were placed in lateral recumbency, with the limb to be blocked positioned uppermost. The transducer was held perpendicular to the most proximal area of the pelvic limb, ventral to the greater trochanter and slightly cranial to the femur. Methylene blue was injected after the localisation of the femoral nerve. Immediately after the injection, the cat was turned and the technique was repeated on the contralateral side. After performing the ‘block’ in both limbs, the area was dissected bilaterally and the success was evaluated. A positive femoral nerve staining was considered for a coverage of ⩾1 cm. Results A total of 10 ultrasound-guided femoral nerve injections were performed. Ninety percent (n = 9/10) of the nerves were successfully dyed. Conclusions and relevance The novel ultrasound-guided lateral approach may be suitable and reproducible for a successful femoral nerve blockade in cats. Further investigations about the clinical usefulness and safety of this femoral nerve block in live cats are required.

scholarly journals Ultrasound-Guided Inferior Alveolar Nerve Block in the Horse: Assessment of the Extraoral Approach in Cadavers

2019 ◽  
Vol 36 (1) ◽  
pp. 46-51
Author(s):  
Jessica Purefoy Johnson ◽  
Robert Karl Peckham ◽  
Conor Rowan ◽  
Alan Wolfe ◽  
John Mark O’Leary
Keyword(s):  
Methylene Blue ◽  
Nerve Block ◽  
Inferior Alveolar Nerve ◽  
Lingual Nerve ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Inferior Alveolar Nerve Block ◽  
Success Rates ◽  
Ultrasound Guided ◽  
Dye Staining

Blinded techniques to desensitize the inferior alveolar nerve (IAN) include intraoral, angled, and vertical extraoral approaches with reported success rates of 100%, 73%, and 59%, respectively. It has not been determined whether an ultrasound-guided extraoral approach is feasible. Further, the fascicular nature of the inferior alveolar and lingual nerves of the horse has not been described. The objectives of this study were to describe a low-volume ultrasound-guided vertical extraoral inferior alveolar nerve block technique and to describe the fascicular nature of these nerves. An ultrasound-guided approach to the IAN was conducted with a microconvex transducer and an 18-G, 15-cm spinal needle using a solution containing iodinated-contrast and methylene blue dye. Accuracy was assessed by contrast visualized at the mandibular foramen on computed tomography (CT) and methylene blue dye staining of the nerves on gross dissection. Sections of inferior alveolar and lingual nerves were submitted for histological analysis. Assessment by CT and dissection determined success rates of 81.3% and 68.8%, respectively; 68.8% of injections had inadvertent methylene blue dye staining of the lingual nerve. Nerve histology revealed both the inferior alveolar and lingual nerves to be multifascicular in nature. Mean fascicle counts for the inferior alveolar and lingual nerves were 29 and 30.8, respectively. The technique is challenging and no more accurate than previously published blinded techniques. Any extraoral approach to the IAN is likely to also desensitize the lingual nerve.

Using of modified-bentonite as low-cost sorbent for removal of methylene blue dye from aqueous solution

2020 ◽  
Vol 27 (2) ◽  
pp. 45-54
Author(s):  
Saraa Muwafaq Ibrahim ◽  
Ziad T. Abd Ali
Keyword(s):  
Aqueous Solution ◽  
Methylene Blue ◽  
Silanol Group ◽  
Infrared Spectrometry ◽  
Blue Dye ◽  
Ammonium Bromide ◽  
Vibration Band ◽  
Order Kinetic ◽  
Methylene Blue Dye ◽  
Modified Bentonite

Batch experiments have been studied to remove methylene blue dye (MB) from aqueous solution using modified bentonite. The modified bentonite was synthesized by replacing exchangeable calcium cations in natural bentonite with cationic surfactant cetyl trimethyl ammonium bromide (CTAB). The characteristics of modified bentonite were studied using different analysis such as Scanning electronic microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and surface area. Where SEM shows the natural bentonite has a porous structure, a rough and uneven appearance with scattered and different block structure sizes, while the modified bentonite surface morphology was smooth and supplemented by a limited number of holes. On other hand, (FTIR) analysis that proved NH group aliphatic and aromatic group of MB and silanol group are responsible for the sorption of contaminate. The organic matter peaks at 2848 and 2930 cm-1 in the spectra of modified bentonite which are sharper than those of the natural bentonite were assigned to the CH2 scissor vibration band and the symmetrical CH3 stretching absorption band, respectively, also the 2930 cm-1 peak is assigned to CH stretching band. The batch study was provided the maximum removal efficiency (99.99 % MB) with a sorption capacity of 129.87 mg/g at specified conditions (100 mg/L, 25℃, pH 11 and 250rpm). The sorption isotherm data fitted well with the Freundlich isotherm model. The kinetic studies were revealed that the sorption follows a pseudo-second-order kinetic model which indicates chemisorption between sorbent and sorbate molecules.

Adsorption Studies of Methylene Blue Dye using Biosorbents

2018 ◽  
Vol 8 (3) ◽  
pp. 502-513
Author(s):  
Saravanan Narayanan ◽  
Rathika Govindasamy
Keyword(s):  
Methylene Blue ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Adsorption Studies
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