scholarly journals Morphological and Histological Features of the Vomeronasal Organ in African Pygmy Hedgehog (Atelerix albiventris)

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1462
Author(s):  
Daisuke Kondoh ◽  
Yusuke Tanaka ◽  
Yusuke K. Kawai ◽  
Takayuki Mineshige ◽  
Kenichi Watanabe ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Periodic Acid ◽  
Vomeronasal Organ ◽  
Sensory Epithelium ◽  
Middle Part ◽  
Periodic Acid Schiff ◽  
Histological Features ◽  
Duct Opening ◽  
Hematoxylin Eosin ◽  
Rostral Part

The vomeronasal organ (VNO) detects specific chemicals such as pheromones and kairomones. Hedgehogs (Eulipotyphla: Erinaceidae) have a well-developed accessory olfactory bulb that receives projections from the VNO, but little is known about the hedgehog VNO. Here, we studied the histological features of the VNO in five individual African pygmy hedgehogs by hematoxylin-eosin, periodic acid-Schiff, and Alcian blue stains. The hedgehog VNO comprises a hyaline cartilage capsule, soft tissue and epithelial lumen, and it branches from the site just before the incisive duct opening into the nasal cavity. The soft tissues contain several small mucous (or mucoserous) glands and a large serous gland, and many venous sinuses all around the lumen. The VNO lumen is round to oval throughout the hedgehog VNO, and the sensory epithelium lines almost the entire rostral part and medial wall of the middle part. These findings indicate that the VNO is functional and plays an important role in the hedgehog. Notably, the VNO apparently has a characteristic flushing mechanism with serous secretions like those of gustatory glands, which the hedgehog might frequently use to recognize the external environment.

scholarly journals Actinomycosis is not Frequent in the Periapex But is a Persistent Lesion

2017 ◽  
Vol 28 (6) ◽  
pp. 688-693 ◽  
Author(s):  
Lucas Senhorinho Esteves ◽  
Águida Cristina Gomes Henriques ◽  
Carolina Ávila Varginha de Moraes e Silva ◽  
Maria Cristina Teixeira Cangussu ◽  
Eduardo Antônio Gonçalves Ramos ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Periodic Acid ◽  
Lesion Size ◽  
Periapical Lesion ◽  
Periodic Acid Schiff ◽  
Bacterial Colony ◽  
Periapical Lesions ◽  
Criteria For Diagnosis ◽  
Hematoxylin Eosin

Abstract Periapical actinomycosis caused by a gram-positive anaerobic pathogen characterizes a typical extra-radicular infection. This study determined the frequency and correlated the content of bacteria colonies with the of periapical actinomycosis size. The study comprised a total of 218 periapical lesions (PL) (cysts, granulomas or abscess). The specimens embedded in paraffin were sliced into 4-µm sections and stained with hematoxylin-eosin, Gram, Periodic Acid-Schiff (PAS) and Grocott’s stain. The presence of bacterial colonies composed of filamentous structures labeled with the histochemical stains were described as Actinomyces, and for each case, the bacterial colonies were counted and measured. The correlation between the number and size of bacterial colonies and the size of PL was tested using Pearson’s adjusted correlation coefficient. From 218 PL, bacterial colonies were identified in 64 biopsies. Seven cases (0.3%) fulfill the criteria for diagnosis of periapical actinomycosis. All of cases were therapy-resistant and did not showed periapical repair after 12 months of follow-up. Periapical surgery or dental extraction was performed. The correlation test indicated no correlation between the number of bacterial colonies and the lesion size (p=0.752, r=-0.148). However, a larger bacterial colony size generally resulted in a larger periapical lesion (P=0.000, r=0.657). The frequency of periapical actinomycosis was low, and this lesion should be included in the differential diagnosis of PL. The size of the Actinomyces colonies seemed to contribute to increase the size of the periapical lesion.

scholarly journals Relationship between the morphologic alterations of vocal cords from adult autopsies and the cause of death

2004 ◽  
Vol 59 (2) ◽  
pp. 63-66 ◽  
Author(s):  
Ana Karina Marques Salge ◽  
Eumenia Costa da Cunha Castro ◽  
Mara Lúcia Fonseca Ferraz ◽  
Marlene Antônia dos Reis ◽  
Vicente de Paula Antunes Teixeira
Keyword(s):  
Vocal Cord ◽  
Cause Of Death ◽  
Respiratory Diseases ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Vocal Cords ◽  
Periodic Acid Schiff ◽  
White Ethnicity ◽  
Staining Methods ◽  
Hematoxylin Eosin

PURPOSE: The purpose of this study was to identify the possible alteration in the thickness of the epithelium basal membrane of the vocal cords and correlate it with the cause of death. METHOD: Larynxes collected from adult autopsies during the period of 1993 to 2001 were utilized. We used the hematoxylin-eosin and periodic acid-Schiff staining methods for the morphological and morphometric analysis. RESULTS: Sixty-six vocal cords were analysed; increased thickness was identified in 14 cases (21.2%), with equal proportions between the genders. Increased vocal-cord thickness was more frequent in patients of the white ethnicity (12 cases, 85.7%). Respiratory alterations were found in 10 (71.4%) of the cases with increased vocal-cord thickness. Of the patients that were maintained with mechanical ventilation before death, 7 (18.4%) had thickening of the basal membrane. Among the smokers, 9 (19.63%) had basal membrane thickening. CONCLUSION: No statistically significant differences were found between the cases in which the cause of death was related to respiratory diseases as compared to non-respiratory diseases and the thickening of the basal membrane of the vocal cords. However, new studies are needed in order to verify the etiopathogenesis of this thickening.

scholarly journals Transcriptomic and microstructural analyses in Liriodendron tulipifera Linn. reveal candidate genes involved in nectary development and nectar secretion

2019 ◽  
Author(s):  
Huanhuan Liu ◽  
Jikai Ma ◽  
Huogen Li
Keyword(s):  
Candidate Genes ◽  
Anthocyanin Biosynthesis ◽  
Expression Profiles ◽  
Periodic Acid ◽  
Middle Part ◽  
Pcr Analysis ◽  
Rna Seq ◽  
Nectar Secretion ◽  
Relict Species ◽  
Periodic Acid Schiff

Abstract Background: Nectar is a major floral attractant and reward for insects that ensures pollination. Liriodendron, a genus of the Magnoliaceae family, includes only two relict species, L. chinense and L. tulipifera, which are considered “basal angiosperms” according to plant evolutionary history. The flowers of Liriodendron plants are insect pollinated and secrete nectar to attract pollinators. To date, the morphology and anatomy of nectaries, the mechanism of nectar secretion and the molecular mechanism of nectary development in Liriodendron remain poorly understood. Methods: In this study, we examined the nectary surface cells and change in starch in L. tulipifera by using scanning electron microscopy and periodic acid-Schiff techniques to select appropriate samples for subsequent research. Transcriptome sequencing was of the top and middle parts of immature nectaries and the middle part of mature and postsecretory nectaries in L. tulipifera was performed. We evaluated the expression profiles of 21 DEGs that are closely related to nectary development and nectar secretion for real-time quantitative PCR analysis. Results: L. tulipifera nectaries are starch-storing nectaries and are located in the top and middle parts of L. tulipifera petals. After analyzing the RNA-seq data, we obtained 115.26 Gb of clean data in 12 libraries and mapped the results to the L. chinense reference genome with 71.02%-79.77% efficiency. In total, 26,955 DEGs were identified by performing six pairwise comparisons. The flavonoid biosynthesis, phenylpropanoid biosynthesis, anthocyanin biosynthesis and starch and sucrose metabolism pathways were enriched and related to nectar secretion and pigment change. We identified 56 transcription factor families, and members of the TCP, Trihelix, C2H2, ERF, and MADS families changed dynamically during nectary development. Moreover, to further verify the accuracy of the RNA-seq results, we validated the expression profiles of 21 candidate genes. Conclusions: We evaluated the nectary development and secretion processes comprehensively and identified many related candidate genes in L. tulipifera. These findings suggest that nectaries play important roles in flavonoid synthesis and petal color presentation.

PATHOLOGY AND RENAL OUTCOME OF THREE UNCOMMON FACES OF CRESCENTRIC GLOMERULONEPHRITIS

2021 ◽  
pp. 7-12
Author(s):  
Keya Basu ◽  
Dipankar Sircar ◽  
Manimoy Bandopadhyay
Keyword(s):  
Iga Nephropathy ◽  
Survival Data ◽  
Periodic Acid ◽  
Rapidly Progressive Glomerulonephritis ◽  
Renal Outcome ◽  
Single Centre ◽  
Periodic Acid Schiff ◽  
Cross Sectional ◽  
Single Centre Study ◽  
Hematoxylin Eosin

AIMS: Rapidly progressive glomerulonephritis (RPGN) presents with rapidly deteriorating renal function (> 50% loss of glomerular ltration rate /GFR within 3 months) associated with nephritic urinary sediments and crescents in biopsy. Crescentric IgA Nephropathy, Anti-GBM (Glomerular basement membrane) disease and combined IgA Nephropathy with Anti-GBM disease are three uncommon reasons of RPGN. We have compared clinicopathological. Immunouorescence (DIF) and renal outcome of three groups. Setting and designs: Prospective, cross-sectional, single centre study. METHODS AND MATERIALS :Ultrasonography guided core biopsies obtained, one stained with hematoxylin-eosin, periodic acid-Schiff, Masson's trichrome, and silver methenamine stain another one with immunouorescence conjugated IgG, IgM, IgA, C3, C1q, kappa and lambda stain. Demographic, clinicopathological and therapeutic parameters with survival data were collected. STATISTICAL ANALYSIS USED: Done using software (GraphPad PRISM 6). RESULTS: We have included 9 cases of crescentric IgA Nephropathies, 6 cases of AntiGBM diseases and 2 cases of combined IgA Nephropathy and AntiGBM diseases. Signicant difference seen in the incidences of hypertension, hemoptysis, serum creatinine, anti GBM antibody, total number of crescents and mesangial hypercellularity, fragmentation of GBM etc. IgG, IgA and kappa positivity in DIF show signicant difference. Survival analysis and mortality versus dialysis dependence and complete and partial remission versus no remission showed no difference between these three groups. CONCLUSIONS: Proper and early clinicopathological diagnosis is important since all are of poor renal outcome. Further renal outcome of the combined disease is same as that of individual ones.

scholarly journals DIFFERENTIAL STAINING OF ULTRATHN SECTIONS OF EPON-EMBEDDED TISSUES FOR LIGHT MICROSCOPY

1965 ◽  
Vol 13 (7) ◽  
pp. 579-582 ◽  
Author(s):  
BERNARD P. LANE ◽  
DOMINIC L. EUROPA
Keyword(s):  
Fine Structure ◽  
Periodic Acid ◽  
Differential Staining ◽  
Tissue Architecture ◽  
Paraffin Sections ◽  
Periodic Acid Schiff ◽  
Thin Sections ◽  
Hematoxylin Staining ◽  
Ultrathin Sections ◽  
Hematoxylin Eosin

A convenient method is described for the removal of Epon 812 from thin sections, utilizing a saturated solutions of sodium hydroxide. The tissue architecture and ultrastructural details are preserved. Hematoxylin-eosin, periodic acid-Schiff and phosphotungstic-hematoxylin staining modifications are suggested which result in differentiation similar to that seen in paraffin sections. The technique is applicable to ultrathin sections suitable for examination with the electron microscope, allowing comparison of staining characteristics and fine structure of adjacent thin sections.

Histoprotective effect of vitamin D against carbon tetrachloride nephrotoxicity in rats

2015 ◽  
Vol 35 (7) ◽  
pp. 713-723 ◽  
Author(s):  
SMEO Elmubarak ◽  
N Özsoy
Keyword(s):  
Vitamin D ◽  
Carbon Tetrachloride ◽  
Periodic Acid ◽  
Kidney Tissue ◽  
Albino Rats ◽  
Periodic Acid Schiff ◽  
Glucose Levels ◽  
Tissue Sections ◽  
Wistar Albino Rats ◽  
Hematoxylin Eosin

This study investigated the protective effect of vitamin D against carbon tetrachloride (CCl4)-induced nephrotoxicity in rats. Adult male Wistar albino rats were divided into four groups ((A) control; (B) 10-week exposure to CCl4; (C) 10-week exposure to CCl4 + vitamin D treatment; and (D) 10-week exposure to CCl4 + 12 weeks of vitamin D treatment). The CCl4 dose (1.5 ml kg−1) was injected subcutaneously twice a week, while the 0.5 mg kg−1 dose of vitamin D was administered intraperitoneally every day, as appropriate for each group. Whole animal and kidney weights as well as serum urea, creatinine, and glucose levels were measured. Kidney tissue sections were stained with hematoxylin–eosin, Masson’s trichrome, and periodic acid–Schiff. Tubular and glomerular degeneration were detected in the kidney tissues of CCl4-treated rats, together with dilatation and vacuolization within the tubules and hemorrhage in the intertubular region. In the kidney glomeruli; congestion, atrophy, and adhesion to parietal layer were observed. Tissue disorganization and aggregation of Bowman’s capsules were noted. Mononuclear cell infiltration was observed between the glomeruli and the tubules. In contrast, the kidney sections and functional parameters of vitamin D-treated rats were similar to the controls, suggesting that vitamin D treatment is able to reduce renal damage.

scholarly journals Morphology of beak and tongue of partrigde Rhynchotus rufescens

2005 ◽  
Vol 35 (5) ◽  
pp. 1098-1102 ◽  
Author(s):  
Juliana Regina Rossi ◽  
Silvana Martinez Baraldi-Artoni ◽  
Daniela Oliveira ◽  
Claudinei da Cruz ◽  
Vanessa Sobue Franzo ◽  
...  
Keyword(s):  
Squamous Epithelium ◽  
Hyaline Cartilage ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Skeletal Musculature ◽  
Stratified Squamous Epithelium ◽  
Filiform Papillae ◽  
Hematoxylin Eosin

Twenty adult partridges Rhynchotus rufescens were used to study the morphology of the beak and the tongue. Lengths of the beak and of the tongue were evaluated, and histologic sections of the tongue were stained routinely with hematoxylin-eosin (HE), periodic acid-Schiff (PAS) and Masson’s trichrome stain, later analyzed and described. The beak of the partridge of both sexes are curved, flat, hard and with a sharp extremity, with mean length of 4.90cm for the females and 4,80 for the males. The tongue is characterized by a triangular format with mean length of 1cm for both sexes, and its extremity is presented as a sharp format. Organization of the tongue tissue presents a stratified squamous epithelium with filiform papillae in all surface, mucous glands with ducts that project to the epithelium surface and a hyaline cartilage in the whole length of the tongue, which lays on the skeletal musculature that is guided in several directions.

scholarly journals Transcriptomic and microstructural analyses in Liriodendron tulipifera Linn. reveal candidate genes involved in nectary development and nectar secretion

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Huanhuan Liu ◽  
Jikai Ma ◽  
Huogen Li
Keyword(s):  
Candidate Genes ◽  
Anthocyanin Biosynthesis ◽  
Expression Profiles ◽  
Periodic Acid ◽  
Middle Part ◽  
Pcr Analysis ◽  
Rna Seq ◽  
Nectar Secretion ◽  
Relict Species ◽  
Periodic Acid Schiff

Abstract Background Nectar is a major floral attractant and reward for insects that ensures pollination. Liriodendron, a genus of the Magnoliaceae family, includes only two relict species, L. chinense and L. tulipifera, which are considered “basal angiosperms” according to plant evolutionary history. The flowers of Liriodendron plants are insect pollinated and secrete nectar to attract pollinators. To date, the morphology and anatomy of nectaries, the mechanism of nectar secretion and the molecular mechanism of nectary development in Liriodendron remain poorly understood. Methods In this study, we examined the nectary surface cells and change in starch in L. tulipifera by using scanning electron microscopy and periodic acid-Schiff techniques to select appropriate samples for subsequent research. Transcriptome sequencing was of the top and middle parts of immature nectaries and the middle part of mature and postsecretory nectaries in L. tulipifera was performed. We evaluated the expression profiles of 21 DEGs that are closely related to nectary development and nectar secretion for real-time quantitative PCR analysis. Results L. tulipifera nectaries are starch-storing nectaries and are located in the top and middle parts of L. tulipifera petals. After analyzing the RNA-seq data, we obtained 115.26 Gb of clean data in 12 libraries and mapped the results to the L. chinense reference genome with 71.02–79.77% efficiency. In total, 26,955 DEGs were identified by performing six pairwise comparisons. The flavonoid biosynthesis, phenylpropanoid biosynthesis, anthocyanin biosynthesis and starch and sucrose metabolism pathways were enriched and related to nectar secretion and pigment change. We identified 56 transcription factor families, and members of the TCP, Trihelix, C2H2, ERF, and MADS families changed dynamically during nectary development. Moreover, to further verify the accuracy of the RNA-seq results, we validated the expression profiles of 21 candidate genes. Conclusions We evaluated the nectary development and secretion processes comprehensively and identified many related candidate genes in L. tulipifera. These findings suggest that nectaries play important roles in flavonoid synthesis and petal color presentation.

scholarly journals 3. MORFOLOGI DAN KANDUNGAN KARBOHIDRAT KELENJAR LINGUALIS DAN SUBLINGUALIS ITIK (Anas plathyrinchos) (Morphology and Carbohydrates Content in Lingual and Sublingual Glands of Duck (Anas plathyrinchos))

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
M Jalaluddin
Keyword(s):  
Secretory Cell ◽  
Periodic Acid ◽  
Secretory Cells ◽  
Sublingual Gland ◽  
Epithelial Layer ◽  
Periodic Acid Schiff ◽  
Tubular Gland ◽  
One Year ◽  
Hematoxylin Eosin ◽  
Carbohydrate Contents

This study was aimed to find out the morphology and carbohydrates content of lingual and sub-lingual glands of duck (Anas plathyrinchos). Sample used in this study was lingual and sublingual glands of a one year old female duck. Anatomy morphology and histology of lingual and sublingual glands were observed after processed into histological preparation and stained with hematoxylin-eosin (HE) then stained further with alcian blue (AB) pH 2.5 and periodic acid Schiff (PAS) to detect carbohydrate contents. The results showed that the lingual gland of duck cannot be observed due to its location below the epithelial layer of the tongue. While the sublingual gland of duck has an elongated shape and not granulated like typically glands, located at ventral tongue (dorsoventral lingual) between os mandibulae and covered with musculus digastricus venter oral. Staining with AB pH 2.5 and PAS showed that cytoplasm of secretory cell and secrete of lingual and sublingual glands contain acidic and neutral carbohydrates which indicated a positive reaction with intensity ranging from weak (+) to strong (+++). This study concluded that the lingual and sublingual gland found in female duck is a complex tubular gland with mucous secretory cells type. ____________________________________________________________________________________________________________________

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