scholarly journals Effect of a Black Soldier Fly Ingredient on the Growth Performance and Disease Resistance of Juvenile Pacific White Shrimp (Litopenaeus vannamei)

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1450
Author(s):  
Andrew Richardson ◽  
João Dantas-Lima ◽  
Maxime Lefranc ◽  
Maye Walraven
Keyword(s):  
Disease Resistance ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Control Diet ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Feed Conversion ◽  
Black Soldier Fly ◽  
Health Performance ◽  
Growth Trial

This study was performed as part of developing a functional feed ingredient for juvenile Pacific white shrimp (Litopenaeus vannamei). Here we assess the effects of dietary inclusion of a Black Soldier Fly Ingredient (BSFI) from defatted black soldier fly (Hermetia illucens) larvae meal on growth performance, tolerance to salinity stress, and disease resistance when challenged with Vibrio parahaemolyticus or a strain of white spot syndrome virus (WSSV). A control diet was used for comparison with three test diets including 4.5, 7.5, and 10.5% of BSFI (BSFI4.5, BSFI7.5, and BSFI10.5). After 28 days, all diets with BSFI had improved weight gain, feed conversion ratio (FCR) and specific growth rate (SGR) compared to control. Indeed, SGR was significantly improved from inclusion of 4.5% in the diet, whilst FCR was significantly improved at 7.5% (p < 0.05). During the growth trial, survival was not affected by diet. Shrimp health performance was not significantly affected by the diets across the disease and salinity challenges. Overall, the results indicate that the inclusion of BSFI from H. illucens improves the performance of juvenile L. vannamei.

scholarly journals The digestibility of biofloc meal from African catfish culture medium as a feed raw material for Pacific white shrimp

2019 ◽  
Vol 18 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Julie Ekasari ◽  
Muhammad Agus Suprayudi ◽  
Putri Elas ◽  
Reza Karunia Senja
Keyword(s):  
Litopenaeus Vannamei ◽  
Dry Matter ◽  
Control Diet ◽  
Basal Diet ◽  
Pacific White Shrimp ◽  
Raw Material ◽  
White Shrimp ◽  
African Catfish ◽  
Feed Conversion ◽  
Dry Matter Digestibility

ABSTRACTThis study was conducted to evaluate the digestibility of biofloc meal collected from catfish culture as a feed raw material for Pacific white shrimp Litopenaeus vannamei diet. A basal feed with 43% crude protein content was used as a control diet and mixed with 2% of binders and 0.5 % of Cr2O3 as a marker for digestibility. The experimental diets were made by mixing 67.5% of the basal diet with 30% of biofloc meal, 2% of binders and 0.5 % of Cr2O3. Nine units of glass tanks (90 cm ×40 cm× 35 cm) filled with 100 L seawater were used as the experimental culture units. White shrimp with an average body weight of 5.61 ± 0.09 g was randomly distributed to each experimental tank at a density of 20 shrimp/tank. The feed was offered at a level of 5% shrimp biomass per day at a frequency of four times a day. The results showed that the dry matter digestibility of feed with 30% biofloc meal in shrimp were similar to that of the reference diet. However, protein and fat digestibility of feed containing biofloc meal were considerably higher than those of the reference diet. Feeding shrimp with 30% biofloc meal diet resulted in higher survival and specific growth rate and lower feed conversion ratio than those of the control. The digestibility of bioflocs dry matter, protein and lipid in Pacific white shrimp obtained in this study were 54.9%, 76.3% and 79.3%, respectively.Keywords: biofloc, digestibility, catfish, shrimp  ABSTRAKPenelitian ini dilakukan untuk mengevaluasi kecernaan tepung bioflok yang diambil dari media pemeliharaan ikan lele sebagai bahan pakan udang vaname Litopenaeus vannamei. Pakan yang mengandung kadar protein 43% digunakan sebagai pakan control dan dicampur dengan 2% binder dan 0.5% Cr2O3 sebagai marker untuk kecernaan. Pakan perlakuan dibuat dengan mencampurkan 67.5% pakan control dengan 30% tepung bioflok, 2% binder dan 0.5% Cr2O3. Penelitian menggunakan sembilan unit akuarium (90 cm ×40 cm ×35 cm) yang diisi 100 L air laut. Udang vaname dengan bobot rata-rata 5.61 ± 0.09 g ditebar secara acak pada setiap akuarium perlakuan pada kepadatan 20 ekor/akuarium. Pakan diberikan dengan tingkat pemberian pakan 5% biomassa per hari sebanyak empat kali sehari. Hasil penelitian ini menunjukkan bahwa kecernaan pakan dengan 30% tepung bioflok tidak berbeda nyata dengan pakan acuan. Namun kecernaan protein dan lemak pakan yang mengandung tepung bioflok terlihat lebih tinggi daripada pakan kontrol. Pemberian pakan dengan tepung bioflok sebanyak 30% juga menghasilkan tingkat kelangsungan hidup, laju pertumbuhan spesifik udang dan rasio konversi pakan yang lebih baik daripada udang yang diberi pakan kontrol. Kecernaan bahan, protein dan lemak tepung bioflok pada udang yang didapat dalam penelitian masing-masing adalah 54.9%, 76.3% dan 79.3%.Kata-kata kunci: bioflok, ikan lele, kecernaan, udang 

scholarly journals Evaluation of the Dietary Black Soldier Fly Larvae Meal (Hermetia illucens) on Growth Performance, Intestinal Health, and Disease Resistance to Vibrio parahaemolyticus of the Pacific White Shrimp (Litopenaeus vannamei)

2021 ◽  
Vol 8 ◽  
Author(s):  
Yongkang Chen ◽  
Shuyan Chi ◽  
Shuang Zhang ◽  
Xiaohui Dong ◽  
Qihui Yang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Microbiota ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Mrna Levels ◽  
Black Soldier Fly ◽  
The Pacific ◽  
Black Soldier Fly Larvae

The present study evaluated the effects of the dietary black soldier fly larvae meal (BSF) on growth performance, intestinal health, and susceptibility to Vibrio parahaemolyticus in the Pacific white shrimp Litopenaeus vannamei. The basal diet was formulated to contain 25% fish meal (FM), and then the FM was replaced with BSF for 10, 20, and 30% of the FM protein in the experimental diets, which are referred to as FM, BSF10, BSF20, and BSF30, respectively. Four hundred and eighty healthy and uniform-sized shrimp (~0.88 g) were distributed among four groups of three replicates, each with 40 shrimp in a 300-L tank and they were fed four times daily for 7 weeks. The results showed that the growth performance did not change significantly in shrimp fed with BSF10 and BSF20 diets, but significantly decreased in those fed with BSF30 diet compared to the ones fed with FM diet. After feeding trial and sampling, a V. parahaemolyticus infection challenge trial was conducted on shrimp. The results showed that the survival rate of shrimp fed with BSF10 was significantly higher than those fed with FM. The results of the midgut histology showed that the width and height of intestinal mucosal folds decreased significantly in shrimp fed with BSF20 and BSF30, and the early signs of apoptosis in the intestinal cells were found in shrimp fed with BSF30. The mRNA levels of non-specific immune-related genes dorsal and relish were downregulated in shrimp fed with BSF20 and BSF30 diets. The mRNA levels of antimicrobial peptides–related genes alf (anti-lipopolysaccharide factor) were upregulated in shrimp fed with BSF10 but downregulated in shrimp fed with BSF30. The mRNA level pen3 (penaeidins 3) was upregulated in shrimp fed with BSF10 and BSF20 diets. The intestinal bacterial communities on operational taxonomic unit levels among groups were not significantly differentiated according to the beta diversity analysis. At the genus levels, a decrease in Vibrio, Photobacterium, and Candidatus_Bacilloplasma, as well as the increase in Bacillus and Pseudoalteromonas abundance, indicated the improvement of intestinal microbiota in shrimp fed with dietary BSF. Therefore, the use of BSF in shrimp diet should be controlled at a dosage of 20% of the FM, which can improve the intestinal microbiota without causing any negative effects.

scholarly journals Digestibility of insect meals for Pacific white shrimp (Litopenaeus vannamei) and their performance for growth, feed utilization and immune responses

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260305
Author(s):  
Jaehyeong Shin ◽  
Kyeong-Jun Lee
Keyword(s):  
Fatty Acids ◽  
Immune Responses ◽  
Litopenaeus Vannamei ◽  
Fish Meal ◽  
Control Diet ◽  
Saturated Fatty Acids ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Black Soldier Fly ◽  
Amino Acid Availability

This study was conducted to examine digestibility of insect meals for Pacific white shrimp (Litopenaeus vannamei) and their utilization as fish meal substitutes. The tested insect meals were mealworm, silkworm, black soldier fly, rice grasshopper, two-spotted cricket, dynastid beetle and white-spotted flower chafer. Apparent digestibility coefficients of the tested insect meals were 83–89% for protein, 91–98% for lipid, 84–90% for energy, 77–81% for dry matter, 28–36% for chitin, 76–96% for amino acids and 89–93% for fatty acids. The amino acid availability of insect meals was high in taurine (93–96%), arginine (91–95%) and lysine (90–95%). Availability of fatty acids were 89–93% for saturated fatty acids, 90–93% for monounsaturated fatty acids and 88–93% for polyunsaturated fatty acids. For a feeding trial, a control diet was formulated using 27% tuna byproduct meal as a fish meal source and seven other diets were prepared replacing 10% tuna byproduct meal in the control diet with each insect meal. Triplicate groups of shrimp (initial body weight: 0.17 g) were fed the diets for 65 days. The growth performance was significantly improved when the shrimp were fed black soldier fly or dynastid beetle included diet. Dietary supplementation of insect meals significantly improved non-specific immune responses and antioxidant enzyme activity in the shrimp. These results indicate that the tested insect meals have high potentials to be used as a protein source that could replace fish meal in diets for the shrimp.

Effect of black soldier fly (Hermetia illucens) larvae meal on lipid and glucose metabolism of Pacific white shrimp Litopenaeus vannamei

2021 ◽  
pp. 1-39
Author(s):  
Yongkang Chen ◽  
Shuyan Chi ◽  
Shuang Zhang ◽  
Xiaohui Dong ◽  
Qihui Yang ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Unsaturated Fatty Acids ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Crude Lipid ◽  
Significant Difference

Abstract The present study investigated the effect of black soldier fly (Hermetia illucens) larvae meal (BSF) on hemolymph biochemical indicators, muscle metabolites as well as the lipid and glucose metabolism of Pacific white shrimp Litopenaeus vannamei. Four diets were formulated in which the control diet contained 25% fishmeal (FM) and 10% (BSF10), 20% (BSF20) and 30% (BSF30) of fishmeal protein were replaced with BSF. 480 shrimp (0.88 ± 0.00 g) were distributed to four groups of three replicates and fed for 7 weeks. Results showed that growth performance of shrimp fed BSF30 significantly decreased compared to those fed FM, but there was no significant difference in survival among groups. The whole shrimp crude lipid (CL) content, hemolymph triglyceride and total cholesterol were decreased with the increasing BSF inclusion. The results of metabolomics showed that the metabolite patterns of shrimp fed different diets were altered, with significant changes in metabolites related to lipid metabolism, glucose metabolism as well as TCA cycle. The mRNA expressions of hk, pfk, pk, pepck, ampk, mcd, cpt-1 and scd1 in hepatopancreas were downregulated in shrimp fed BSF30, but mRNA expression of acc1 was upregulated. Unlike BSF30, the mRNA expressions of fas, cpt-1, fbp and 6pgd in hepatopancreas were upregulated in shrimp fed BSF20. This study indicates that BSF20 diet promoted lipid synthesis and lipolysis, while BSF30 diet weakened β-oxidation and glycolysis as well as affected the unsaturated fatty acids synthesis, which may affect the growth performance and body composition of shrimp.

Effects of conjugated linoleic acid and curcumin on growth performance and oxidative stress enzymes in juvenile Pacific white shrimp ( Litopenaeus vannamei ) feed with aflatoxins

2019 ◽  
Vol 51 (3) ◽  
pp. 1051-1060
Author(s):  
Oscar Daniel García‐Pérez ◽  
Mireya Tapia‐Salazar ◽  
Martha G. Nieto‐López ◽  
Julio César Cruz‐Valdez ◽  
Maribel Maldonado-Muñiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Linoleic Acid ◽  
Growth Performance ◽  
Conjugated Linoleic Acid ◽  
Litopenaeus Vannamei ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Oxidative Stress Enzymes ◽  
Stress Enzymes ◽  
And Oxidative Stress
Sign in / Sign up

Export Citation Format

Share Document