scholarly journals Long-Term Preservation and Storage of Faecal Samples in Whatman® Cards for PCR Detection and Genotyping of Giardia duodenalis and Cryptosporidium hominis

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1369
Author(s):  
Pamela Carolina Köster ◽  
Begoña Bailo ◽  
Alejandro Dashti ◽  
Carolina Hernández-Castro ◽  
Rafael Calero-Bernal ◽  
...  
Keyword(s):  
Giardia Duodenalis ◽  
Cost Effective ◽  
Cold Chain ◽  
Cryptosporidium Hominis ◽  
Long Term Storage ◽  
Faecal Samples ◽  
Effective Option ◽  
Control Procedures ◽  
And Storage

Preservation and conservation of biological specimens, including faecal samples, is a challenge in remote areas or poor-resource settings where the cold chain cannot be maintained. This study aims at evaluating the suitability of filter cards for long-term storage of faecal samples of animal and human origin positive to the diarrhoea-causing protozoan parasites, Giardia duodenalis and Cryptosporidium hominis. Three commercially available Whatman® Filter Cards were comparatively assessed: the FTA® Classic Card, the FTA® Elute Micro Card, and the 903 Protein Saver Card. Human faecal samples positive to G. duodenalis (n = 5) and C. hominis (n = 5) were used to impregnate the selected cards at given storage (1 month, 3 months, and 6 months) periods and temperature (−20 °C, 4 °C, and room temperature) conditions. Parasite DNA was detected by PCR-based methods. Sensitivity assays and quality control procedures to assess suitability for genotyping purposes were conducted. Overall, all three Whatman® cards were proven useful for the detection and molecular characterisation of G. duodenalis and C. hominis under the evaluated conditions. Whatman® cards represent a simple, safe, and cost-effective option for the transportation, preservation, and storage of faecal samples without the need of the cold chain.

scholarly journals The Study of Soybean Chaff Pressing Process Depending on Its Fractional Composition and Humidity

2019 ◽  
Vol 5 (5) ◽  
pp. 160-164 ◽  
Author(s):  
V. Shishkin ◽  
E. Shulzhenko
Keyword(s):  
Fractional Composition ◽  
Complex Mixture ◽  
Cost Effective ◽  
Laboratory Unit ◽  
Long Term Storage ◽  
The Cost ◽  
Far Eastern ◽  
And Storage ◽  
Term Storage

A by-product of soybean production, as a non-grain part of the crop, can be used to meet the needs of livestock in providing complete feeding of livestock. Pressing the floor allows to reduce the volume of plant material in order to reduce the cost of storage and transportation, improve the safety of its nutrients during long-term storage. Therefore, the development of an efficient, cost-effective and less energy-intensive technology and a process line for compacting the soybean floor by pressing is a promising direction. The pile of the floor is a complex mixture of particles of different sizes, as well as voids filled with air. Equipment compaction chaff should provide a stable preparation of pellets under all the fluctuations of the moisture content and fractional composition of chaff. In 2018, research experiments were carried out in the Far Eastern Scientific–Research Institute of Mechanization and Electrification of Agriculture to study the process of pressing the soybean floor, depending on its fractional composition and humidity, on the manufactured laboratory unit for compaction with the production of soybean floor briquettes. It was found that when the humidity of the sample increases from 9 to 21%, the energy consumption decreases by 17.1%. The coefficient of compaction of the floor when the humidity changes almost does not change. By increasing the length of the sex particles from 14 to 87 mm, the compaction coefficient increases by 73.3% and the energy intensity by 6.2 %. For transportation and storage of the soybean floor briquette, it is recommended to pack it tightly with polyethylene film or tie it with twine, this will increase the shelf life and eliminate losses.

MODERN PROBLEMATICS AND ANALYSIS OF EFFICIENCY OF APPLICATION OF DIFFERENT APPROACHES TO DESTRUCTION OF INFORMATION ON MAGNETIC MEDIA

2019 ◽  
Vol 2 (12) ◽  
pp. 154-164
Author(s):  
O. Semenenko ◽  
O. Vodchyts ◽  
V. Koverga ◽  
R. Lukash ◽  
O. Lutsenko
Keyword(s):  
Armed Forces ◽  
Information Storage ◽  
Storage Device ◽  
Magnetic Media ◽  
Long Term Storage ◽  
Physical Impact ◽  
And Storage ◽  
Term Storage

The introduction and active use of information transmission and storage systems in the Ministry of Defense (MoD) of Ukraine form the need to develop ways of guaranteed removal of data from media after their use or long-term storage. Such a task is an essential component of the functioning of any information security system. The article analyzes the problems of guaranteed destruction of information on magnetic media. An overview of approaches to the guaranteed destruction of information on magnetic media of different types is presented, and partial estimates of the effectiveness of their application are given by some generally accepted indicators of performance evaluation. The article also describes the classification of methods of destruction of information depending on the influence on its medium. The results of the analysis revealed the main problems of application of software methods and methods of demagnetization of the information carrier. The issue of guaranteed destruction of information from modern SSD devices, which are actively used in the formation of new systems of information accumulation and processing, became particularly relevant in the article. In today's conditions of development of the Armed Forces of Ukraine, methods of mechanical and thermal destruction are more commonly used today. In the medium term, the vector of the use of information elimination methods will change towards the methods of physical impact by the pulsed magnetic field and the software methods that allow to store the information storage device, but this today requires specialists to develop new ways of protecting information in order to avoid its leakage.

Seeds as Economical Production Platform for Recombinant Proteins

2020 ◽  
Vol 27 (2) ◽  
pp. 89-104 ◽  
Author(s):  
Muhammad Sarwar Khan ◽  
Faiz Ahmad Joyia ◽  
Ghulam Mustafa
Keyword(s):  
Oral Delivery ◽  
High Protein ◽  
Biologically Active ◽  
Cold Chain ◽  
Protein Accumulation ◽  
Long Term Storage ◽  
Production Platform ◽  
Vaccine Antigens ◽  
Term Storage

: The cost-effective production of high-quality and biologically active recombinant molecules especially proteins is extremely desirable. Seed-based recombinant protein production platforms are considered as superior choice owing to lack of human/animal pathogenic organisms, lack of cold chain requirements for transportation and long-term storage, easy scalability and development of edible biopharmaceuticals in plants with objective to be used in purified or partially processed form is desirable. This review article summarizes the exceptional features of seed-based biopharming and highlights the needs of exploiting it for commercial purposes. Plant seeds offer a perfect production platform for high-value molecules of industrial as well as therapeutic nature owing to lower water contents, high protein storage capacity, weak protease activity and long-term storage ability at ambient temperature. Exploiting extraordinarily high protein accumulation potential, vaccine antigens, antibodies and other therapeutic proteins can be stored without effecting their stability and functionality up to years in seeds. Moreover, ability of direct oral consumption and post-harvest stabilizing effect of seeds offer unique feature of oral delivery of pharmaceutical proteins and vaccine antigens for immunization and disease treatment through mucosal as well as oral route.

scholarly journals Rediscovering Zygorhizidium affluens Canter: Molecular Taxonomy, Infectious Cycle, and Cryopreservation of a Chytrid Infecting the Bloom-Forming Diatom Asterionella formosa

2018 ◽  
Vol 84 (23) ◽  
Author(s):  
Cecilia Rad-Menéndez ◽  
Mélanie Gerphagnon ◽  
Andrea Garvetto ◽  
Paola Arce ◽  
Yacine Badis ◽  
...  
Keyword(s):  
Molecular Taxonomy ◽  
Cost Effective ◽  
Trophic Levels ◽  
Content Type ◽  
Long Term Storage ◽  
Asterionella Formosa ◽  
Infectious Cycle ◽  
Cryopreservation Method ◽  
Term Storage

ABSTRACT Parasitic Chytridiomycota (chytrids) are ecologically significant in various aquatic ecosystems, notably through their roles in controlling bloom-forming phytoplankton populations and in facilitating the transfer of nutrients from inedible algae to higher trophic levels. The diversity and study of these obligate parasites, while critical to understand the interactions between pathogens and their hosts in the environment, have been hindered by challenges inherent to their isolation and stable long-term maintenance under laboratory conditions. Here, we isolated an obligate chytrid parasite (CCAP 4086/1) on the freshwater bloom-forming diatom Asterionella formosa and characterized its infectious cycle under controlled conditions. Phylogenetic analyses based on 18S, 5.8S, and 28S ribosomal DNAs (rDNAs) revealed that this strain belongs to the recently described clade SW-I within the Lobulomycetales. All morphological features observed agree with the description of the known Asterionella parasite Zygorhizidium affluens Canter. We thus provide a phylogenetic placement for this chytrid and present a robust and simple assay that assesses both the infection success and the viability of the host. We also validate a cryopreservation method for stable and cost-effective long-term storage and demonstrate its recovery after thawing. All the above-mentioned tools establish a new gold standard for the isolation and long-term preservation of parasitic aquatic chytrids, thus opening new perspectives to investigate the diversity of these organisms and their physiology in a controlled laboratory environment. IMPORTANCE Despite their ecological relevance, parasitic aquatic chytrids are understudied, especially due to the challenges associated with their isolation and maintenance in culture. Here we isolated and established a culture of a chytrid parasite infecting the bloom-forming freshwater diatom Asterionella formosa. The chytrid morphology suggests that it corresponds to the Asterionella parasite known as Zygorhizidium affluens. The phylogenetic reconstruction in the present study supports the hypothesis that our Z. affluens isolate belongs to the order Lobulomycetales and clusters within the novel clade SW-I. We also validate a cryopreservation method for stable and cost-effective long-term storage of parasitic chytrids of phytoplankton. The establishment of a monoclonal pathosystem in culture and its successful cryopreservation opens the way to further investigate this ecologically relevant parasitic interaction.

scholarly journals Chlorogenic acid content in potato tubers with colored flesh as affected by a genotype, location and long-term storage

2019 ◽  
Vol 65 (No. 7) ◽  
pp. 355-360
Author(s):  
Matyáš Orsák ◽  
Karel Hamouz ◽  
Jaromír Lachman ◽  
Pavel Kasal
Keyword(s):  
Chlorogenic Acid ◽  
Acid Content ◽  
Field Experiments ◽  
The Other ◽  
Potato Tubers ◽  
Flesh Color ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

In three-year field experiments, the effect of genotype, flesh color, site conditions and storage on chlorogenic acid content (CAC) in tubers of potato cultivars with purple or red flesh was compared to yellow-fleshed cv. Agria. The results confirmed the significant effect of genotype on CAC. The highest CAC was characteristic on a three-year mean for the purple-fleshed cv. Vitelotte (769.5 mg/kg fresh weight (FW)), i.e. 1.19−2.6 times higher than in the other cultivars. In regard to the effect of flesh color, significantly higher mean CAC levels have been shown for the red-fleshed (2.8 times) and purple-fleshed (3.16 times) cultivars in comparison with cv. Agria (148 mg/kg FW). At the Uhříněves location with a warmer climate and frequent dry periods as compared to the second Valečov location, a higher CAC (1.18 times) was found. Cold storage (4°C, 6 months) resulted in a significant CAC increase varying from 33.2% in the Blaue St. Galler cultivar to 210.6% in the Vitelotte cultivar among all eight evaluated color-fleshed cultivars. On the other hand, the effect of storage on CAC was not evident in the yellow-fleshed Agria cultivar (inconclusive difference against CAC after harvest).

scholarly journals Aboveground storage of hydrocarbon gas hydrates

2018 ◽  
Vol 60 ◽  
pp. 00028
Author(s):  
Mykola Zotsenko ◽  
Larysa Pedchenko ◽  
Andrii Manhura
Keyword(s):  
Gas Hydrate ◽  
Mechanical Characteristics ◽  
Negative Temperature ◽  
Prolonged Storage ◽  
Long Term Storage ◽  
Hydrocarbon Gas ◽  
Mixing Method ◽  
And Storage ◽  
Term Storage

The work objective is to substantiate the technical bases for the production of gas hydrate blocks directly on-site for further transportation and storage in above ground storages, as an alternative to transporting and storing gas in underground gas storages. The theoretical bases have been considered and processes of gas hydrate blocks producing, physical-mechanical characteristics of artificial hydrates with the purpose of determining the conditions of their storage and prolonged storage were tested in laboratory conditions. The construction of above ground gas hydrate storage in the form of a building, partially deepened in a ground, which is separated from the environment by a wall of gravel cement elements made by the mixing method were substantiated. It is proved that such a constructive solution for a long-term storage of gas hydrate blocks is the most economical in terms of energy consumption to maintain internal negative temperature.

scholarly journals Long-Term Storage of Compound Solutions for High-Throughput Screening by Using a Novel 1536-Well Microplate

2009 ◽  
Vol 14 (5) ◽  
pp. 492-498 ◽  
Author(s):  
Martin Joseph Pfeifer ◽  
Guenther Scheel
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Cost Effective ◽  
The Novel ◽  
Long Term Storage ◽  
Plate Design ◽  
Minor Losses ◽  
Storage Times ◽  
Term Storage

This report describes the features and the performance of a new and significantly improved 1536-well microplate design. The design allows for simple, automation-friendly, and cost-effective storage of compound solutions for high-throughput screening. The plate design is based on Society for Biomolecular Sciences standards for microplates and can be molded from polystyrene or cycloolefin copolymer, thus making the plate suitable for use with acoustic dispensing as well as other conventional liquid dispensing in the nanoliter range. For a 9:1 DMSO/water mix as solvent, the novel plate design has shown to perform over 4 months with only minor losses in solvent. Thus, this novel plate design creates the basis for further reductions in compound storage volumes and allows for an increase in the storage times for microliter volumes for up to a year or more. The high protection against solvent evaporation is also visible for aqueous solutions, thus allowing for reduced edge effects during screening campaigns.

Nutrient losses in cereal and pasture hays stored in three types of hay packages in the open in two environments

1985 ◽  
Vol 25 (2) ◽  
pp. 326 ◽  
Author(s):  
MJ Cochrane ◽  
RB Wickes
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Losses ◽  
Storage Medium ◽  
Long Term Storage ◽  
Large Round ◽  
And Storage ◽  
Term Storage ◽  
Lower Medium

Cereal and pasture hays, cut at the same site, were packaged into medium-sized round bales, large round bales and stacks. Halfthe number of each hay type and of each hay package were stored in the open for up to 15 months in a 500 mm rainfall area and half were transported to and stored for up to 15 months in a 1000 mm rainfall area. Densities of the pasture hay packages were within the manufacturers' specified ranges but those of the cereal hay packages were lower. Medium-sized round bales were less dense than large round bales and weathered more than large round bales and stacks after 10 and 15 months storage. Cereal hay packages deteriorated more than pasture hay packages with long-term storage. Medium-sized round bales lost more dry matter than large round bales after storage over the winter and more digestible dry matter over this period and after long-term storage. They also lost more crude protein than large round bales when stored over the first summer and over the following winter. It was economic to store all packages in the open over the first summer. It was uneconomic to store medium-sized round bales in the open over the following winter, while storage of large round bales and stacks was economic. For long-term storage, it was uneconomic to store any of the hay packages in the open, and storage under cover would have to be considered.

Relationship between Acoustic Firmness and Magness Taylor Firmness in Royal Gala and Golden Smoothee Apples

2009 ◽  
Vol 15 (1) ◽  
pp. 31-40 ◽  
Author(s):  
D. Molina-Delgado ◽  
S. Alegre ◽  
J. Puy ◽  
I. Recasens
Keyword(s):  
Quality Parameters ◽  
Acoustic Measurements ◽  
Good Tool ◽  
Malus Domestica Borkh ◽  
Long Term Storage ◽  
Acoustic Methods ◽  
The Relationship ◽  
And Storage ◽  
Term Storage

This study aimed to evaluate apple firmness measured using both the penetrometer and acoustic methods. The methodologies were applied to Royal Gaya and Golden Smoothee apples (Malus domestica. Borkh) harvested from 12 different orchards in Catalonia (Spain), on six different dates, and over three seasons. The relationship between firmness and physicochemical quality parameters was analyzed at harvest and post-harvest. The results obtained showed a noticeable correlation between Magness Taylor firmness and acoustic measurements in Royal Gala, but no correlation was found for Golden Smoothee. Fruit variety and storage had a clear influence on correlations between the two measurements. Measurements of Magnes Taylor and acoustic firmness seemed to correspond to different physical properties. Acoustic measurements seemed to be a good tool for evaluating changes in tissue firmness during long-term storage, especially for Golden Smoothee apples. The maturity of the fruit seemed to play a more important role in the measurement of Magnes Taylor firmness than in the measurement of acoustic firmness for the Royal Gaya variety.

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