scholarly journals Association between Anti-Müllerian Hormone Concentration and Inflammation Markers in Serum during the Peripartum Period in Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1241
Author(s):  
Hiroaki Okawa ◽  
Danielle Monniaux ◽  
Chihiro Mizokami ◽  
Atsushi Fujikura ◽  
Toshihiro Takano ◽  
...  
Keyword(s):  
Vaginal Discharge ◽  
Serum Amyloid A ◽  
Hormone Concentration ◽  
Inflammation Markers ◽  
Somatic Cell Counts ◽  
Amyloid A ◽  
Cell Counts ◽  
Esterified Fatty Acids ◽  
Significant Difference ◽  
Peripartum Period

The relationships between changes in anti-Müllerian hormone (AMH) concentration and various traits, including milk somatic cell counts (SCC), were evaluated. Blood samples were collected from 43 Holstein cows 14 days before (D-14) and 10 (D10) and 28 days after (D28) parturition, and vaginal discharge score (VDS) and polymorphonuclear leukocyte (PMNL) percentages were assessed in endometrial samples at D28. Cows were separated into four quartiles (Q1–Q4) based on changes in AMH concentration during the peripartum period (AMH ratio: D28/D-14). Correlations between AMH ratio and each parameter were evaluated and classified into high-AMH (Q4, 1.83 ± 0.12, n = 11) and low-AMH (Q1, 0.83 ± 0.05, n = 11) groups. The AMH ratio was positively correlated with magnesium and non-esterified fatty acids levels, and the albumin/globulin ratio at D10 and D28, but negatively correlated with serum amyloid A (SAA) at D10. SAA and γ-globulin levels were significantly higher in the low-AMH group at D28. There was no significant difference in VDS, PMNL percentage, and milk SCC between the two groups. The decreasing AMH ratio from the prepartum to the postpartum period corresponds to high inflammation biomarker levels. Whether it subsequently affects the reproductive prognosis of postpartum cows needs further investigations.

scholarly journals Relationship between some variables of protein profile and indicators of lipomobilization in dairy cows after calving

2014 ◽  
Vol 57 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Csilla Tóthová ◽  
Oskar Nagy ◽  
Gabriel Kováč
Keyword(s):  
Dairy Cows ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Protein Profile ◽  
Total Proteins ◽  
Amyloid A ◽  
Esterified Fatty Acids ◽  
Positive Correlations ◽  
Altered Lipid Metabolism ◽  
Altered Lipid

Abstract. The objective of this study was to determine the concentrations of the main indicators of lipomobilization and selected variables of protein profile in dairy cows after calving, including immunoglobulins and acute phase proteins, as well as to evaluate the relationships between the altered lipid metabolism and changes in protein profile. Into the evaluation we included 54 clinically healthy dairy cows of a Slovak spotted breed, low-land black spotted breed and their crossbreeds in the period of 1-2 weeks after parturition. Blood samples were analysed for non-esterified fatty acids (NEFA, mmol/l), β-hydroxybutyrate (BHB, mmol/l), total proteins (TP, g/l), albumin (Alb, g/l), immunoglobulin G (IgG, g/l), haptoglobin (Hp, g/l) and serum amyloid A (SAA, mg/l). In cows with concentrations of NEFA above 0.35 mmol/l (n=20) we found significantly lower mean serum concentrations of total proteins, albumin and IgG than in cows with serum NEFA concentrations below 0.35 mmol/l (n=34) (P<0.001). On the other hand, cows with higher values of NEFA showed significantly higher mean concentrations of BHB, Hp and SAA (P<0.001). The concentrations of NEFA significantly negatively correlated with the values of TP (P<0.001), albumin (P<0.01) and IgG (P<0.001). Significant positive correlations were found between the concentrations of NEFA and BHB, Hp, as well as SAA (P<0.001). Similar correlations were also found between the values of BHB and the variables of protein profile except for albumin. This study indicates strong relationships between NEFA and selected variables of protein profile in cows after parturition.

Acute Phase Proteins (Haptoglobin and Serum Amyloid A) and Pro-Inflammatory Cytokines (IL-12 and IL-10) in Cows Vaccinated with Prototype Killed S. aureus Mastitis Vaccine and Challenged with S. aureus Mastitis Infection.

2020 ◽  
Author(s):  
Idris Umar Hambali ◽  
Faez Firdaus Jesse Bin Abdallah ◽  
Khaleequl rahaman Bhuttu ◽  
Azmi M Lila ◽  
zunita Zakaria ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Serum Amyloid ◽  
Bacterial Challenge ◽  
Amyloid A ◽  
Significant Difference ◽  
Group B ◽  
Pro Inflammatory Cytokines ◽  
Friesian Cows

Abstract Background The economic downturn experienced by farmers and the fear of milk borne infection are of a greater public health concern. Haptoglobin, Serum Amyloid A, IL-12 and IL-10 in lactating Friesian cows vaccinated with prototype killed S. aureus mastitis vaccine and challenged with S. aureus were evaluated. Bacterin concentration at 10 8 cfu /ml of the local isolate of S. aureus was adjuvanted with KAl(SO₄)₂. Six lactating Friesian cows were grouped into A= Negative control, B = Positive control and C = vaccine group. Group C was vaccinated intramuscularly with 2ml of the monovalent vaccine, groups A and B with physiologic normal saline. Groups B and C were later challenged with the live bacterium via intramammary route . Result There was a significant increase in IL-10 concentration in vaccinated group post primary vaccination (PPV), booster phase (PB) and during the bacterial challenge phase. There was also a significantly increased IL-12 concentration in the vaccinated group at 24 hours, weeks 1 and 2 PPV. Haptoglobin at 12 and 24 hours PPV had a significant difference in group C. During the PB at 8 and 12 hours there was a significant difference in group C. During the bacterial challenged phase at 0, 3, 24 hours and day 7 PC there was a significant difference in group B. At 8 hours PC there was a significant difference in group C. For Serum Amyloid A, during PPV at 0, 3, 8, 12, 24 hours and weeks 1 and 2, the concentrations was significantly different in groups C. During PB at 0, 3, 8 and 12 hours PB there was a significant difference in groups C. During the bacterial challenge phase at 3, 8, 12, 24 hours, days 7 and 14 PC there was a significant difference in group B. At 0 hour PC there was a significant increase observed in group C. Conclusion The developed prototype killed S. aureus mastitis vaccine using local isolates was able to stimulate acute phase proteins and pro-inflammatory cytokines. The pattern of responses PC indicated protection, thereby suggesting that vaccination can protect against mastitis infection in dairy cows.

scholarly journals Physiotherapy protocol during initial postoperative period of arthroscopy in horses

2018 ◽  
Vol 38 (12) ◽  
pp. 2201-2206
Author(s):  
Fernanda C. Stievani ◽  
Thais S.L. Machado ◽  
Kaio B. Bezerra ◽  
Marilene M. Silva ◽  
Raquel Y.A. Baccarin ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Physical Examination ◽  
Postoperative Period ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
Control Group ◽  
Physical Analysis ◽  
Amyloid A ◽  
Significant Difference ◽  
Significant Change

ABSTRACT: This study evaluated the effects of a physiotherapy protocol applied in joints with osteochondritis dissecans submitted to arthroscopy. Twelve horses totaling twenty joints were used and divided into two uniform groups, according to articular lesion grade. Treated Group (TG) received the physiotherapy protocol (cryotherapy, passive rage motion and controlled exercise) that initiate just after anesthetic recovery and extended for five days. Control Group (CG) remained resting in stall during the same period. Physical examination and synovial fluid analysis were used to evaluate the treatment. The synovial fluid examination consisted of physical analysis (color, aspect, and viscosity), mucin clot evaluation, Serum Amyloid A, Prostaglandin E2 and urea concentration. Synovial samples were collected by arthrocentesis at the beginning of the surgical procedure (D1), 48 hours (D3) and 96 hours (D5) after surgery. Before arthroscopy and daily during the postoperative period joints were evaluated by physical exam: superficial temperature (°C), range of motion (degrees) and circumference (centimeters). The joint physical examination showed no significant difference between groups and neither along the days for the same group. The parameters of synovial fluid showed difference over the moments in each group but didn’t have difference between groups. Color and aspect had the same patterns across moments, in CG fluid had significant change when compared D1 with D3 (color and aspect: p<0.001) and D5 (color: p<0.001; aspect: p<0.05) becoming mostly bloody and cloudy in D3 and D5. However in TG the difference was significant just between D1 and D3 (color and aspect: p<0.05), showing an improvement of synovial fluid in D5 (color and aspect: p>0.05). Viscosity and mucin clot evaluation showed significant change in CG between D1 and D3 (viscosity: p<0.01; mucin clot: p<0.05) and between D1 and D5 (viscosity: p<0.01;mucin clot: p<0.01). In TG no significant difference of viscosity and mucin clot was observed over the moments, showing an early improvement of synovial fluid quality. The Serum Amyloid A concentration showed an extremely significant increase in CG (p<0.001) when compared D1 (1217.13±664.47μg/mL) and D3 (42423.80±52309.31μg/mL). The comparison between D1 and D5 in CG, and across moments in TG, had no statistical difference. The PGE2 eicosanoid remained statistically unchanged all over the time. Urea showed significant increase in D3 when compared to D1 (p<0.001) in CG, and had no variation in TG. The physiotherapy protocol minimized the inflammatory mediators and provided minor alterations in synovial fluid after arthroscopy.

scholarly journals Can blood serum amyloid A concentrations in horses differentiate synovial sepsis from extrasynovial inflammation and determine response to treatment?

2020 ◽  
Vol 187 (6) ◽  
pp. 235-235
Author(s):  
Matthew Sinovich ◽  
Nicolas F Villarino ◽  
Ellen Singer ◽  
Claire S Robinson ◽  
Luis M Rubio-Martínez
Keyword(s):  
Serum Amyloid A ◽  
Prognostic Indicator ◽  
Diagnostic Value ◽  
Serum Amyloid ◽  
Response To Treatment ◽  
Specific Marker ◽  
Time Curve ◽  
Serial Blood ◽  
Amyloid A ◽  
Significant Difference

BackgroundSerum amyloid A (SAA) concentrations in blood and synovial fluid of horses with synovial sepsis have diagnostic value. Studies suggest serial blood SAA measurements could act as a prognostic indicator. This study evaluated the use of serial blood SAA concentrations for monitoring of horses with synovial sepsis.MethodsA prospective clinical trial was performed of horses referred to a single hospital with synovial sepsis that survived (n=17), synovial sepsis that were euthanised (n=5), non-septic intrasynovial pathologies (n=14) or extensive extrasynovial lacerations (n=5). SAA concentrations were determined on admission and every 24 hours thereafter. The area under the concentration–time curve from 0 to 144 hours of each group was compared by Kruskal-Wallis and post hoc Dunn’s tests (P<0.05).ResultsSignificant difference in mean blood concentration of SAA was found between synovial sepsis that survived and non-septic pathologies in the first 48 hours, as well as between non-septic intrasynovial pathologies and non-responsive sepsis requiring euthanasia. No difference was found between extensive extrasynovial lacerations and any septic group.ConclusionsWhile serial blood SAA is useful for monitoring clinical response of intrasynovial septic pathologies, interpretation should consider other clinical findings since blood SAA is not a specific marker for synovial sepsis.

Abstract 388: Impact of Individual Acute Phase Serum Amyloid A Isoforms on HDL Metabolism in Mice

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Maria C De Beer ◽  
Myung-Hee Kim ◽  
Joanne M Wroblewski ◽  
Richard C Charnigo ◽  
Ailing Ji ◽  
...  
Keyword(s):  
Acute Phase ◽  
Plasma Clearance ◽  
Serum Amyloid A ◽  
Biological Activities ◽  
Acute Phase Reactant ◽  
Serum Amyloid ◽  
Minor Role ◽  
Clearance Rates ◽  
Amyloid A ◽  
Significant Difference

The acute phase reactant serum amyloid A (SAA) is an HDL apolipoprotein that exhibits biological activities as a pro-inflammatory mediator, but its physiological function(s) are poorly understood. Possible functional differences between SAA1.1 and SAA2.1, the two major SAA isoforms, are also unclear. Mice deficient in either SAA1.1 or SAA2.1 were used to investigate SAA isoform plasma clearance rates and effects on HDL structure, composition and apolipoprotein catabolism. The absence of either isoform did not affect the size of the normally enlarged HDL found in acute phase wild type mice, and did not result in significant changes in HDL lipid composition. Plasma clearance rates of normal and acute phase HDL apolipoproteins were determined using native HDL particles. The fractional clearance rates (FCR’s) of apoA-I, apoA-II and SAA were distinct, indicating that neither normal nor acute phase particles are cleared as intact particles. No significant difference was found between the FCR’s of SAA1.1 and SAA2.1 in acute phase mice, suggesting that the selective deposition of SAA1.1 observed in amyloid plaques is not associated with a difference in the rates of plasma clearance of the isoforms. In the absence of the HDL receptor SR-BI, the clearance rate of SAA was reduced by about 30% and remained significantly greater compared to that of apoA-I and apoA-II, indicating a relatively minor role of SR-BI in SAA clearance. These studies contribute to our understanding of the metabolism of SAA and its effects on acute phase HDL composition and catabolism.

Effects of Yerba Mate (Ilex paraguariensis) supplementation on the productive performance of dairy cows during mid-lactation

2010 ◽  
Vol 50 (6) ◽  
pp. 339 ◽  
Author(s):  
Pietro Celi ◽  
Herman W. Raadsma
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Ilex Paraguariensis ◽  
Yerba Mate ◽  
Reactive Oxygen Metabolites ◽  
Blood Samples ◽  
Somatic Cell Counts ◽  
Cell Counts ◽  
Esterified Fatty Acids ◽  
Oxygen Metabolites

Yerba Mate (Ilex paraguariensis), a tea known for its high antioxidant content, was fed (250 g/cow.day) to 8 of 16 Holstein cows for 6 weeks to assess its effect on their performance. Cows were weighed and blood samples were taken on Weeks 0, 3 and 6. Blood samples were centrifuged and plasma was analysed for reactive oxygen metabolites, biological antioxidant potential, advanced oxidation protein products and non-esterified fatty acids. Cows were milked two times daily and milk yields were recorded daily for individual cows. On Weeks 0 and 6, individual milk samples were collected from two consecutive milkings, composited, and analysed for somatic cell counts, fat and true protein concentrations. Plasma concentrations of reactive oxygen metabolites, biological antioxidant potential and non-esterified fatty acids were not affected by Yerba Mate supplementation. Similarly, no effect of Yerba Mate supplementation was noted on milk fat and protein content and on somatic cell counts. This study indicates that supplementation of dairy cows’ diet with Yerba Mate during mid lactation seems to improve milk yield when cows are fed with maize silage; however, even if the effect on milk yield was significant it was quite small and needs to be validated with further studies. Cows’ oxidative status was not affected by Yerba Mate supplementation indicating that the effect of Yerba Mate on their productive performances is not mediated by changes in redox status.

scholarly journals Serum amyloid A and muscle activity biomarkers in horses submitted to equestrian show jumping

2019 ◽  
Vol 39 (8) ◽  
pp. 668-671 ◽  
Author(s):  
Wilson P. Carvalho Filho ◽  
Leandro A. Fonseca ◽  
Fabricia M. Girardi ◽  
Lucas D. Bento ◽  
Pollyanna C. Souto ◽  
...  
Keyword(s):  
Muscle Activity ◽  
Repeated Measures ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Significant Difference ◽  
Aspartate Amino Transferase ◽  
Jumping Exercise ◽  
Level Of Significance ◽  
Show Jumping

ABSTRACT: The aim of this study was to evaluate the serum amyloid A (SAA) and biomarkers of muscle activity of horses submitted to show jumping activity. To do this, the variables SAA, glucose, lactate and the biomarkers creatine kinase (CK) and aspartate amino transferase (AST) were evaluated in 10 horses submitted to the show jumping exercise in a tournament for beginners. The evaluations occurred before exercise (T0), immediately after (T1), 30 minutes (T2), 60 minutes (T3) and 24 hours after the end (T4). Data were evaluated using analysis of variance for repeated measures. The statistical software SAEG 9.1 was used to verify the level of significance between the moments for P<0.05. Glucose presented a difference between the moments T0 (97.7±13.3mg/dL) and T1 (79.7±14.1mg/dL). Lactate presented elevation in T1 (15.3±6.1mmol/L) compared to the others T0 (3.8±0.8mmol/L), T2 (6.5±3.9mmol/L), T3 (5.3±2.2mmol/L) and T4 (5.1±1.6mmol/L). The CK showed a significant difference between T0 (82.8±51.2U/L) and T1 (140.1±58.5U/L) and between T4 (74.4±43.1U/L) with T1 (140.1±58.5U/L). The AST presented no difference between moments. The show jumping activity with one-meter obstacles did not induce changes in the SAA protein between the moments.

scholarly journals 5 Effect of rest stop on welfare indicators of conditioned and non-conditioned ranch-direct or auction-market-sourced beef cattle, transported by road

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 3-3
Author(s):  
Daniela M Meléndez ◽  
Sonia Marti ◽  
Timothy Schwinghamer ◽  
Derek B Haley ◽  
Wiolene M Nordi ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Dry Matter ◽  
Serum Amyloid A ◽  
Road Transport ◽  
Beef Calves ◽  
Behavioral Indicators ◽  
Auction Market ◽  
Amyloid A ◽  
N Source ◽  
Esterified Fatty Acids

Abstract The aim of this study was to assess the effect of an 8h rest stop after 36 h of road transport on physiological and behavioral indicators of welfare in 7–8-mo-old conditioned and non-conditioned beef calves, sourced from either a ranch or an auction market. Three hundred and twenty weaned calves (245 ± 35.7 kg BW) were randomly assigned to a 2 × 2 × 2 factorial design: conditioning, conditioned (C) or non-conditioned (N); source, ranch direct (R) or auction market (A); and rest, 0 (R0) or 8 (R8) h. After resting, animals were transported for an additional 4 h. A subset of 12 animals/treatment were sampled for non-esterified fatty acids (NEFA), serum amyloid-A (SAA) and creatine kinase (CK) concentrations prior to the first loading (L1); after 36 h of transport; prior to and after the additional 4 h of transport, on the day of arrival (0); and 1, 2, 3, 5, 14, and 28 d after the 4h transport. Daily standing % and dry matter intake (DMI) were assessed for 3 d after transport. Data were analyzed using the GLIMMIX procedure of SAS. Fixed effects included conditioning, source, and time (nested in rest), while random effects were animal and pen. At L1, mean concentrations of NEFA, SAA and CK were greater (P &lt; 0.05) for N-R0 than C-R0 and for N-R8 than C-R8. The N and R groups had greater (P &lt; 0.05) standing % than the C and A groups, respectively. On d 0 and 1, the C-R0 and C-R8 groups had greater (P &lt; 0.05) DMI than the N-R0 and N-R8 groups, respectively. Overall, physiological and behavioral indicators of welfare were greater in conditioned than non-conditioned calves, while fewer differences were observed between ranch and auction market calves, as well as rested or unrested calves.

Sign in / Sign up

Export Citation Format

Share Document