scholarly journals Prevalence of Dichelobacter nodosus and Ovine Footrot in German Sheep Flocks

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1102
Author(s):  
Julia Storms ◽  
Anna Wirth ◽  
Danae Vasiliadis ◽  
Isabelle Brodard ◽  
Antje Hamann-Thölken ◽  
...  
Keyword(s):  
Field Study ◽  
Real Time ◽  
Causative Agent ◽  
Real Time Pcr ◽  
Scoring System ◽  
Visual Inspection ◽  
Dichelobacter Nodosus ◽  
Virulent Strains

The bacterium Dichelobacter nodosus (D. nodosus) is the causative agent of ovine footrot. The aim of this field study was to determine the prevalence of D. nodosus in German sheep flocks. The sheep owners participated voluntarily in the study. More than 9000 sheep from 207 flocks were screened for footrot scores using a Footrot Scoring System from 0 to 5 and sampling each sheep using one interdigital swab for all four feet of the sheep. The detection and discrimination between benign and virulent strains was done employing a real-time PCR. Our results showed a mean prevalence of 42.93% of D. nodosus in German sheep on an animal level. Underrunning of hoof horn on at least one foot (Scores 3-5) was detected in 567 sheep (6.13%). Sheep with four clinically healthy feet were found through visual inspection in 47.85% of all animals included in this study. In total, 1117 swabs from sheep with four clinically healthy feet tested positive for D. nodosus. In 90.35% of the positive swabs, virulent D. nodosus were detected. Benign D. nodosus were detected in 4.74% of the D. nodosus-positive swabs while 4.91% tested positive for both, benign and virulent D. nodosus. In 59 flocks D. nodosus were not detected and in 115 flocks only virulent D. nodosus were found while seven flocks tested positive for benign strains.

Application of real-time PCR for early diagnosis of diseases caused by Aeromonas salmonicida, Vibrio anguillarum, and Tenacibaculum maritimum in turbot: A field study

2017 ◽  
Vol 30 (1) ◽  
pp. 76-89 ◽  
Author(s):  
María-José Chapela ◽  
Martiña Ferreira ◽  
Asela Ruiz-Cruz ◽  
Iris Martin-Varela ◽  
Jacobo Fernández-Casal ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Field Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Diagnosis Of Diseases

Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB)

2008 ◽  
Vol 99 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Sang-Hoon Han ◽  
Do-Bu Lee ◽  
Dong-Woo Lee ◽  
Eul-Hwan Kim ◽  
Byoung-Su Yoon
Keyword(s):  
Real Time ◽  
Causative Agent ◽  
Real Time Pcr ◽  
Paenibacillus Larvae ◽  
American Foulbrood

scholarly journals Pooling of interdigital swab samples for PCR detection of virulent Dichelobacter nodosus

2017 ◽  
Vol 30 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Deborah Greber ◽  
Iwan Locher ◽  
Peter Kuhnert ◽  
Marc-André Butty ◽  
Kerstin Holdener ◽  
...  
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Substantial Reduction ◽  
Control Program ◽  
Foot Rot ◽  
Dichelobacter Nodosus ◽  
Foot Disease ◽  
Development And Validation ◽  
Adequate Method

Virulent ovine foot rot is a contagious foot disease. Given the development and validation of a real-time PCR to detect Dichelobacter nodosus isolates that contain the virulence-associated protease genes aprV2 and aprB2, the diagnosis of foot rot has made considerable progress. We evaluated pooling methods to reduce the number of samples during a foot rot control program. Samples of individual feet were compared to a 4-feet sample of the same sheep. All further analyses based on 4-feet samples (pools-of-5 and pools-of-10 4-feet samples) were compared to samples of individual sheep, and a risk-based herd sampling was evaluated and compared to the whole flock. The sensitivity and specificity of the 4-feet samples for detection of aprV2-positive strains was 93.8% (CI: 87.6–97.5%) and 98.3% (CI: 96.5–99.3%), respectively. The sensitivity and specificity of the pools-of-10 was 86.7% (CI: 78.4–92.7%) and 100.0% (CI: 97.4–100%), respectively. Pools-of-5 were not significantly more sensitive than pools-of-10. The pooling of 4 individual foot samples into one 4-feet sample is an adequate method to reduce the number of samples of individual sheep. The sensitivity of pools-of-5 and pools-of-10 is too imprecise for a control program. Risk-based sampling allowed for a substantial reduction of samples to be tested, had a sensitivity of 95.8% (CI: 78.9–99.9%) and specificity of 100.0% (CI: 88.1–100.0%) when determining the foot rot flock status, and represents an adequate methodology to predict within-flock freedom from infection.

scholarly journals Identification ofNeisseria gonorrhoeaeas the Causative Agent in a Case of Culture‐Negative Dermatitis–Arthritis Syndrome Using Real‐Time PCR

2008 ◽  
Vol 15 (5) ◽  
pp. 369-371 ◽  
Author(s):  
Patrick T. Kimmitt ◽  
Andrew Kirby ◽  
Nelun Perera ◽  
Karl G. Nicholson ◽  
Paul C. Schober ◽  
...  
Keyword(s):  
Real Time ◽  
Causative Agent ◽  
Real Time Pcr ◽  
Culture Negative

scholarly journals Field study on bovine paratuberculosis using real-time PCR and liquid culture for testing environmental and individual fecal samples implemented in dairy cow management

2019 ◽  
Vol 102 (12) ◽  
pp. 11260-11267
Author(s):  
A.K. Schwalm ◽  
C. Metzger-Boddien ◽  
G. Seemann ◽  
J. Mandl ◽  
A. Obiegala ◽  
...  
Keyword(s):  
Field Study ◽  
Real Time ◽  
Dairy Cow ◽  
Real Time Pcr ◽  
Liquid Culture ◽  
Fecal Samples
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