scholarly journals The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 936
Author(s):  
Qi Mang ◽  
Jilun Hou ◽  
Tian Han ◽  
Guixing Wang ◽  
Yufen Wang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Paralichthys Olivaceus ◽  
Hormone Secretion ◽  
Japanese Flounder ◽  
Integrated Analysis ◽  
Histological Structure ◽  
Hormone Regulation ◽  
Non Coding Rna ◽  
Signal Transduction Networks ◽  
Liver Structure

The liver can synthesize vitellogenin, the precursor of vitellin, which is needed for oocyte development and maturation. Here, we investigated the effects of infertility on liver structure, hormone regulation, and gene and protein networks in Japanese flounder (Paralichthys olivaceus). Results showed that the liver of infertile fish had fewer vacuoles and significantly lower serum vitellogenin (VTG) level than in liver of fertile fish. Whole transcriptomics analysis between infertile and fertile groups identified 2076 significantly differentially expressed (DE) mRNAs, 431 DE lncRNAs, 265 DE circRNAs, and 53 DE miRNAs. Proteomics analysis identified 838 DE proteins. Integrated analysis of whole transcriptomics and proteomics revealed 60 significantly DE genes and proteins associated with metabolism, immunity, signal transduction, and steroid biosynthesis. Moreover, non-coding RNA (miRNAs, circRNA, and lncRNA) transcripts involved in metabolism, immunity, and signal transduction in infertile liver were identified. In conclusion, this study shows that gonadal infertility is associated with not only changes in histological structure and hormone secretion but also changes in metabolism, immunity, and signal transduction networks in the liver. These results provide valuable information concerning the mechanism underlying infertility in fish.

scholarly journals The integrated analysis of RNA-seq and microRNA-seq depicts miRNA-mRNA networks involved in Japanese flounder (Paralichthys olivaceus) albinism

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0181761 ◽  
Author(s):  
Na Wang ◽  
Ruoqing Wang ◽  
Renkai Wang ◽  
Yongsheng Tian ◽  
Changwei Shao ◽  
...  
Keyword(s):  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Integrated Analysis ◽  
Rna Seq

scholarly journals Micro-Transcriptome Analysis Reveals Immune-Related MicroRNA Regulatory Networks of Paralichthys olivaceus Induced by Vibrio anguillarum Infection

2020 ◽  
Vol 21 (12) ◽  
pp. 4252
Author(s):  
Xianhui Ning ◽  
Li Sun
Keyword(s):  
Immune Response ◽  
Transcriptome Analysis ◽  
Regulatory Networks ◽  
Expression Profiles ◽  
Paralichthys Olivaceus ◽  
Vibrio Anguillarum ◽  
Japanese Flounder ◽  
Integrated Analysis ◽  
Immune Gene ◽  
Hub Genes

MicroRNAs (miRNAs) are non-coding regulatory RNAs that play a vital part in the host immune response to pathogen infection. Japanese flounder (Paralichthys olivaceus) is an important aquaculture fish species that has suffered from bacterial diseases, including that caused by Vibrio anguillarum infection. In a previous study, we examined the messenger RNA (mRNA) expression profiles of flounder during V. anguillarum infection and identified 26 hub genes in the flounder immune response. In this study, we performed the micro-transcriptome analysis of flounder spleen in response to V. anguillarum infection at 3 different time points. Approximately 277 million reads were obtained, from which 1218 miRNAs were identified, including 740 known miRNAs and 478 novel miRNAs. Among the miRNAs, 206 were differentially expressed miRNAs (DEmiRs), and 104 of the 206 DEmiRs are novel miRNAs identified for the first time. Most of the DEmiRs were strongly time-dependent. A total of 1355 putative target genes of the DEmiRs (named DETGs) were identified based on integrated analysis of miRNA-mRNA expressions. The DETGs were enriched in multiple functional categories associated with immunity. Thirteen key DEmiRs and 66 immune DETGs formed an intricate regulatory network constituting 106 pairs of miRNAs and DETGs that span five immune pathways. Furthermore, seven of the previously identified hub genes were found to be targeted by 73 DEmiRs, and together they formed interlinking regulatory networks. These results indicate that V. anguillarum infection induces complicated miRNA response with extensive influences on immune gene expression in Japanese flounder.

scholarly journals Integrated Analysis of mRNA and Non-coding RNA Transcriptome in Pepper (Capsicum chinense) Hybrid at Seedling and Flowering Stages

2021 ◽  
Vol 12 ◽  
Author(s):  
Huang-ying Shu ◽  
He Zhou ◽  
Hai-ling Mu ◽  
Shu-hua Wu ◽  
Yi-li Jiang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Transcription Factor ◽  
Regulatory Networks ◽  
Plant Hormone ◽  
Signal Transduction Pathway ◽  
Integrated Analysis ◽  
Capsicum Chinense ◽  
Phenotypic Data ◽  
Non Coding Rna ◽  
Micro Rnas

Pepper is an important vegetable in the world. In this work, mRNA and ncRNA transcriptome profiles were applied to understand the heterosis effect on the alteration in the gene expression at the seedling and flowering stages between the hybrid and its parents in Capsicum chinense. Our phenotypic data indicated that the hybrid has dominance in leaf area, plant scope, plant height, and fruit-related traits. Kyoto Encyclopedia of Genes and Genomes analysis showed that nine members of the plant hormone signal transduction pathway were upregulated in the seedling and flowering stages of the hybrid, which was supported by weighted gene coexpression network analysis and that BC332_23046 (auxin response factor 8), BC332_18317 (auxin-responsive protein IAA20), BC332_13398 (ethylene-responsive transcription factor), and BC332_27606 (ethylene-responsive transcription factor WIN1) were candidate hub genes, suggesting the important potential role of the plant hormone signal transduction in pepper heterosis. Furthermore, some transcription factor families, including bHLH, MYB, and HSF were greatly over-dominant. We also identified 2,525 long ncRNAs (lncRNAs), 47 micro RNAs (miRNAs), and 71 circle RNAs (circRNAs) in the hybrid. In particular, downregulation of miR156, miR169, and miR369 in the hybrid suggested their relationship with pepper growth vigor. Moreover, we constructed some lncRNA–miRNA–mRNA regulatory networks that showed a multi-dimension to understand the ncRNA relationship with heterosis. These results will provide guidance for a better understanding of the molecular mechanism involved in pepper heterosis.

Integrated analysis of non-coding RNA and mRNA expression profiles of 2 pig breeds differing in muscle traits

2017 ◽  
Vol 95 (3) ◽  
pp. 1092 ◽  
Author(s):  
J. Sun ◽  
M. Xie ◽  
Z. Huang ◽  
H. Li ◽  
T. chen ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Expression Profiles ◽  
Integrated Analysis ◽  
Pig Breeds ◽  
Non Coding Rna

Mapping QTLs for principle components of growth traits using distorted markers in Japanese flounder Paralichthys olivaceus

2017 ◽  
Vol 24 (3) ◽  
pp. 440
Author(s):  
Ning LI ◽  
Liyi ZHANG ◽  
Ting LI ◽  
Yanhong LI ◽  
Haijin LIU ◽  
...  
Keyword(s):  
Growth Traits ◽  
Paralichthys Olivaceus ◽  
Japanese Flounder

The Phosphoinositide Signal Transduction Pathway in the Pathogenesis of Alzheimer’s Disease

2018 ◽  
Vol 15 (4) ◽  
pp. 355-362 ◽  
Author(s):  
Vincenza Rita Lo Vasco
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Signal Transduction ◽  
Membrane Trafficking ◽  
Hormone Secretion ◽  
Signal Transduction Pathway ◽  
Brain Morphology ◽  
Signal Transduction Pathways ◽  
Transduction Pathway ◽  
Cell Functions

Background: During aging and in age-associated disorders, such as Alzheimer's Disease (AD), learning abilities decline. Probably, disturbances in signal transduction in brain cells underlie the cognitive decline. The phosphorylation/dephosphorylation imbalance occurring in degenerating neurons was recently related to abnormal activity of one or more signal transduction pathways. AD is known to be associated with altered neuronal Ca<sup>2+</sup> homeostasis, as Ca<sup>2+</sup> accumulates in affected neurons leading to functional impairment. It is becoming more and more evident the involvement of signal transduction pathways acting upon Ca<sup>2+</sup> metabolism and phosphorylation regulation of proteins. A growing interest raised around the role of signal transduction systems in a number of human diseases including neurodegenerative diseases, with special regard to the systems related to the phosphoinositide (PI) pathway and AD. The PI signal transduction pathway plays a crucial role, being involved in a variety of cell functions, such as hormone secretion, neurotransmitter signal transduction, cell growth, membrane trafficking, ion channel activity, cytoskeleton regulation, cell cycle control, apoptosis, cell and tissue polarity, and contributes to regulate the Ca<sup>2+</sup> levels in the nervous tissue. Conclusion: A number of observations indicated that PI-specific phospholipase C (PLC) enzymes might be involved in the alteration of neurotransmission. To understand the role and the timing of action of the signalling pathways recruited during the brain morphology changes during the AD progression might help to elucidate the aetiopathogenesis of the disease, paving the way to prognosis refinement and/or novel molecular therapeutic strategies.

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